[Objective] The aim was to explore the effects of seven kinds of Chinese herbal medicine on Escherichia coil and Staphylococcus aureus in terms of unilater- al bacteriostatic effect, such as root and leaf of Ophiopogo...[Objective] The aim was to explore the effects of seven kinds of Chinese herbal medicine on Escherichia coil and Staphylococcus aureus in terms of unilater- al bacteriostatic effect, such as root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, and Allium macrostemon. [Method] Herbal active ingredients were extracted by water boiling method and a bacteriostatic experiment was per- formed by oxford cup diffusion method, to select Chinese herbs with antibacterial effects. MIC values of seven kinds of Chinese herbal medicine were determined by in vitro half-fold dilution method. [Results] Root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, Allium macrostemon, Juncus effusus and Xanthi- um sibiricum had bacteriostatic effects on Escherichia coil and Staphylococcus au- reus in varying degrees. Xanthium and leaf of Ophiopogon japonicus performed bet- ter on Escherichia coil, with MIC values at 7.81 and 15.63 mg/ml, respectively. Panax pseudoginseng and Xanthium performed better on Staphylococcus aureus with MIC values at 15.63 and 31.25 mg/ml. And the drug＇s effect was positively correlated with the concentration. [Conclusion] Xanthium sibiricum is the best bacte- riostatic effect, followed by leaf of Ophiopogon japonicus, and Juncus effusus per- forms the poorest. Panax pseudoginseng showed the best bacteriostatic effect, fol- lowed by Xanthium sibiricum and Juncus effusus performs the poorest. These pro- vide references for clinic safety.展开更多
[Objective] The aim was to research effects of P fertilizer and lime on growth of Trrifolium repens, Chamaecrista rotundifolia and Macroptilium atropur- pureum, to provide references for cultivation of the three plant...[Objective] The aim was to research effects of P fertilizer and lime on growth of Trrifolium repens, Chamaecrista rotundifolia and Macroptilium atropur- pureum, to provide references for cultivation of the three plants. [Method] Pot experiments were conducted with Trrifolium repens, Charnaecrista rotundifolia and Macroptilium atropurpureum in 2010 in order to research effects of lime and P fer-tilizer mixture on growth of the plants in southern hilly acidic red soils. [Result] With lime amount fixed, application of P fertilizer would enhance plant height, total tiller number and dry matter. When P fertilizer was not applied, however, plant height of the three plants achieved the peak by lime at 1.4 g/kg which proved best for improvement of acidity of red soils. With P fertilizer at 200 mg/kg was applied, biomass of Trifolium repens and Macroptilium atropurpureum achieved the highest by lime at 2.1 g/kg, but total biomass of Chamaecrista rotundifolia was the highest by lime at 1.4 g/kg. [Conclusion] The research provides references for planting and production of Trifolium repens, Chamaecrista rotundifolia and Macroptilium atropur-pureum in southern hilly regions.展开更多
In vertebrates, non-lens βγ-crystallins are widely expressed in various tissues and their functions are not well known. The molecular mechanisms of trefoil factors （TFFs）, which involved in mucosal healing and tum...In vertebrates, non-lens βγ-crystallins are widely expressed in various tissues and their functions are not well known. The molecular mechanisms of trefoil factors （TFFs）, which involved in mucosal healing and tumorigenesis, have remained elusive. βγ-CAT is a novel multifunctional protein complex of non-lens βγ-crystallin and trefoil factor from frog skin secretions. Here we report that βγ-CAT could induce sustained contraction of isolated rabbit aortic rings in dosage （2-35nmol/L） and endothelium dependent manners （P〈0.01 ）. In addition, in situ immunofluorescence indicated that positive TNF-α signals were mainly detected at the endothelial cell layer of βγ-CAT （25nmol/L） treated rings. Furthermore, βγ-CAT induced primary cultured rabbit thoracic aortic endothelial cells （RAECs） rapidly to release TNF-α. After βγ-CAT （25nmol/L） treated for 10 and 30min, the levels of the endothelial cells released TNF-ct were 34.17±5.10 pg/mL and 98.01±4.67 pg/mL （P〈0.01）, respectively. In conclusion, βγ-CAT could induce sustained contraction of isolated aortic rings, and the contractile effect might be partially explained by the release of TNF-α. These findings will give new insight into understanding the functions and physiological roles of non-lens βγ-crystallins and trefoil factors.展开更多
[Objective] The aim was to study response of chlorophyll fluorescence parameters in Trifolium repens L.leaves to water stress from perspective of physiology.[Method] With T.repens cultivar ＂Haifa＂ as tested material...[Objective] The aim was to study response of chlorophyll fluorescence parameters in Trifolium repens L.leaves to water stress from perspective of physiology.[Method] With T.repens cultivar ＂Haifa＂ as tested material,three soil water content levels were set to culture plants,including 75% （no stress,CK）,50% （moderate stress,LD） and 25% （severe stress,HD）,the effects of water stress on chlorophyll fluorescence parameters were determined.[Result] The results showed that the chlorophyll fluorescence parameters changed a little when the relative soil moisture content was 75% and 50%,while the chlorophyll fluorescence parameters such as conversion efficiency of primary light energy （Fv/Fm） of PS Ⅱ,the photochemical quenching coefficient （qP） and the quantum yield of PS Ⅱ electron transport （φPS Ⅱ） decreased respectively when the relative soil moisture content was 25%.[Conclusion] When T.repens in severe drought conditions,the physiological functions were damaged,showing symptoms of drought injury.展开更多
Two new triterpenoid saponins, monepaloside K (1) and monepaloside L (2), together with a known saponin, mazusaponin I (3), were isolated from the water-soluble part of the whole plant of Marina nepalensis var. alba H...Two new triterpenoid saponins, monepaloside K (1) and monepaloside L (2), together with a known saponin, mazusaponin I (3), were isolated from the water-soluble part of the whole plant of Marina nepalensis var. alba Hand.-Mazz. The structures of monepalosides K and L were determined to be 3-O-α-L-arabinopyranosyl-(1-3)-α-D-xylopyranosyl siaresinolic acid (1) and 3-O-β-D-glucopyranosyl-( 1-3)-β-L-arabinopyranosy1 siaresinolic acid 28-O-β-D-glucopyranosyl-(1-6)-β-D-glucopyranoside (2) respectively, on the basis of chemical and spectroscopic evidence.展开更多
[Objective]The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province.[ Method] The root tip of A. macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, disso...[Objective]The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province.[ Method] The root tip of A. macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, dissociated and stained for preparing the glass slide to reveal the chromosome number via the microscopic examination; the sparse cells with good chromosome morphology were photographed under microscope. [ Result ]Allium macrostemon Bunge in Laizhou City introduced in this study was tdploid; its somatic chromosome number was 24 and karyotype formula was K（2n） =3x=24m（2SAT） ＋ 1Bs, thus the karyotype belongs to 1A type. One of the chromosome No. 3 contained satellite, and chromosome deletion may be existed in one of the chromosome No. 5. In addition, B chromosome was observed in some cells.[ Conclusion] This introduction of triploid A. macrostemon found in China was the first time.展开更多
[Objective] The study aimed to explore the degradation law and trend of artificial grassland. [Method] Taking the ryegrass （Lolium perenne） - white clover （ Trifolium repens） artificial grassland in Maiping Townsh...[Objective] The study aimed to explore the degradation law and trend of artificial grassland. [Method] Taking the ryegrass （Lolium perenne） - white clover （ Trifolium repens） artificial grassland in Maiping Township, Guizhou Province as the research object, the grassland vegetation of 40 quadrate from different areas （area around the sheep shed, hilltop, hillside, flatland at the foot of the hill） were analyzed by comparing the dominance and richness index. [ Result] Degradation of different degrees appeared in various areas of this artificial grassland. To be specific, the degradation of grassland around the sheep shed was not serious for less consumption by the sheep; however, the areas on the hilltop and hillside degenerated to the grassland types of dallis grass （ Paspalum dilatatum Poir. ） -Indian lovegrass （ Eragrostis pilosa） and dallis grass - cogongrass [ Imperata cylindrica （ Linn. ） Beauv. ], respectively, and the area at the foot of the hill degenerated to the grassland type dominated by garland chrysanthetnum （ Chrysanthemum coronarium L. ） and knotgrass （ Paspalum distichum L. ）. [ Conclusion ] This study provided a basis for grassland improvement as well as the efficient and sustainable utilization of grazing-type artificial grassland in South China.展开更多
[Objective] The aim was to select and identify inorganic phosphorus-dissolving bacteria in rhizosphere of Trifolium repens,and to research related effects of growth promotion.[Method] Phosphate solubilizing bacteria i...[Objective] The aim was to select and identify inorganic phosphorus-dissolving bacteria in rhizosphere of Trifolium repens,and to research related effects of growth promotion.[Method] Phosphate solubilizing bacteria in rhizosphere of Trifolium repens in Guizhou Province were separated and selected to determine its ability of phosphate solubilizing and IAA secretion,and growth promotion effect.[Result] Phosphate solubilizing bacteria were multiple and varied in rhizosphere of Trifolium repens;solubilizing quantities of phosphorus from calcium phosphate by RW2,RW6 and RW18 were 337.39,447.45 and 462.03 μg/ml;solubilizing quantity was none of linear relation with organic acid;RW2 proved the one secreted most IAA at 12.69 μg/ml,followed by RW18 at 12.34 μg/ml;the three strains were all of Leclercia sp.,but were of none pathogenicity for seedlings of Trifolium repens,instead,a kind of growth promotion was found.Detailedly,RW18 was most effective in growth promotion for Trifolium repens and plant height,root length and weight,and aboveground biomass increased by 110.98%,9.17%,46.17% and 2% respectively.Hence,RW18 is of great potential.[Conclusion] The research provides scientific references for developing phosphate solubilizing bacteria and compound inoculation and develops a novel way to relieve barren soils,save phosphorus resource,reduce environment pollution,and guarantee sustainable development of agriculture and animal husbandry.展开更多
Chouioia cunea Yang is a natural enemy for many kinds of crop and forest pests, particularly for Hyphantria cunea Drary, which is an international quarantine pest. The experiment of rearing Chouioia cunea with Tussah ...Chouioia cunea Yang is a natural enemy for many kinds of crop and forest pests, particularly for Hyphantria cunea Drary, which is an international quarantine pest. The experiment of rearing Chouioia cunea with Tussah (Silkworm) pupa were carried out by 搕hree-cut method?and inoculating method. The results showed that three-cut method is effective way for breed-ing Chouioia cunea, with a parasitical rate of 95%. The biological characteristics and the life cycle of Chouioia cunea were ob-served and described and more hosts of Chouioia cunea were found. 揟hree-cut method?as a new technique of rearing Chouioia cunea has been put into practice.展开更多
A number of disorders have been described to cause protein losing enteropathy (PLE) in children. Primary intestinal lymphangiectasia (PIL) is one mechanism leading to PLE. Few syndromes are associated with PIL; Hennek...A number of disorders have been described to cause protein losing enteropathy (PLE) in children. Primary intestinal lymphangiectasia (PIL) is one mechanism leading to PLE. Few syndromes are associated with PIL; Hennekam syndrome (HS) is one of them. The principal treatment for PIL is a high protein, low fat diet with medium chain triglycerides supplementation. Supportive therapy includes albumin infusion. Few publications have supported the use of octreotide to diminish protein loss and minimize hypoalbuminemia seen in PIL. There are no publications on the treatment of PIL with octreotide in patients with HS. We report two children with HS and PLE in which we used octreotide to decrease intestinal protein loss. In one patient, octreotide increased serum albumin to an acceptable level without further need for albumin infusions. The other patient responded more dramatically with near normal serum albumin levels and cessation of albumin infusions. In achieving a good response to octreotide in both patients, we add to the publications supporting the use of octreotide in PIL and suggest that octreotide should be tried in patients with PIL secondary to HS. To the best of our knowledge, this is the first case report on the use of octreotide in HS-associated PIL.展开更多
Heterotrimeric G proteins are known to function as messengers in numerous signal transduction pathways.The nullmutation of RGA(rice heterotrimeric G protein α subunit),which encodes the α subunit of heterotrimeric G...Heterotrimeric G proteins are known to function as messengers in numerous signal transduction pathways.The nullmutation of RGA(rice heterotrimeric G protein α subunit),which encodes the α subunit of heterotrimeric G proteinin rice,causes severe dwarfism and reduced responsiveness to gibberellic acid in rice.However,less is known aboutheterotrimeric G protein in brassinosteroid(BR)signaling,one of the well-understood phytohormone pathways.In thepresent study,we used root elongation inhibition assay,lamina inclination assay and coleoptile elongation analysis todemonstrated reduced sensitivity of dl mutant plants(caused by the null mutation of RGA)to 24-epibrassinolide(24-epiBL),which belongs to brassinosteroids and plays a wide variety of roles in plant growth and development.Moreover,RGA transcript level was decreased in 24-epiBL-treated seedlings in a dose-dependent manner.Our results show thatRGA is involved in rice brassinosteroid response,which may be beneficial to elucidate the molecular mechanisms of Gprotein signaling and provide a novel perspective to understand BR signaling in higher plants.展开更多
AIM： To investigate the expression and activity of leukotriene C4 （LTC4） synthesis enzymes and their underlying relationship with cysteinyl leukotriene （cys-LT） generation in a rat fulminant hepatic failure （FHF...AIM： To investigate the expression and activity of leukotriene C4 （LTC4） synthesis enzymes and their underlying relationship with cysteinyl leukotriene （cys-LT） generation in a rat fulminant hepatic failure （FHF） model induced by D-galactosamine/lipopolysaccharide （D-GaIN/ LPS）. METHODS： Rats were treated with D-GaIN （300 mg/kg） plus LPS （0.1 mg/kg） for 1, 3, 6, and 12 h. Enzyme immunoassay was used to determine the hepatic cys-LT content. Reverse transcription-polymerase chain reaction （RT-PCR）, Western blot or immunohistochemical assay were employed to assess the expression or location of LTC4 synthesis enzymes, which belong to membrane associated proteins in eicosanoid and glutathione （MAPEG） metabolism superfamily. Activity of LTC4 synthesis enzymes was evaluated by determination of the products of LTA4 after incubation with liver microsomes using high performance liquid chromatography （HPLC）. RESULTS： Livers were injured after treatment with D-GaIN/LPS, accompanied by cys-LT accumulation at the prophase of liver injury. Both LTC4 synthase （LTC4S） and microsomal glutathione-S-transferase （mGST） 2 were expressed in the rat liver, while the latter was specifically located in hepatocytes. Their mRNA and protein expressions were up-regulated at an earlier phase after treatment with D-GaIN/LPS. Meantime, a higher activity of LTC4 synthesis enzymes was detected, although theactivity of LTC4S played the main role in this case. CONCLUSION： The expression and activity of both LTC4S and mGST2 are up regulated in a rat FHF model, which are, at least, partly responsible for cys-LT hepatic accumulation.展开更多
文摘[Objective] The aim was to explore the effects of seven kinds of Chinese herbal medicine on Escherichia coil and Staphylococcus aureus in terms of unilater- al bacteriostatic effect, such as root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, and Allium macrostemon. [Method] Herbal active ingredients were extracted by water boiling method and a bacteriostatic experiment was per- formed by oxford cup diffusion method, to select Chinese herbs with antibacterial effects. MIC values of seven kinds of Chinese herbal medicine were determined by in vitro half-fold dilution method. [Results] Root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, Allium macrostemon, Juncus effusus and Xanthi- um sibiricum had bacteriostatic effects on Escherichia coil and Staphylococcus au- reus in varying degrees. Xanthium and leaf of Ophiopogon japonicus performed bet- ter on Escherichia coil, with MIC values at 7.81 and 15.63 mg/ml, respectively. Panax pseudoginseng and Xanthium performed better on Staphylococcus aureus with MIC values at 15.63 and 31.25 mg/ml. And the drug＇s effect was positively correlated with the concentration. [Conclusion] Xanthium sibiricum is the best bacte- riostatic effect, followed by leaf of Ophiopogon japonicus, and Juncus effusus per- forms the poorest. Panax pseudoginseng showed the best bacteriostatic effect, fol- lowed by Xanthium sibiricum and Juncus effusus performs the poorest. These pro- vide references for clinic safety.
基金Supported by National Science & Technology Pillar Program(2012BAD05B05)Special Fund for Agro-scientific Research in the Public Interest(201303139)+1 种基金国家科技支撑计划"中低产田改良科技工程"项目(2012BAD05B05)农业部公益性行业科研专项经费项目(201303139)资助
文摘[Objective] The aim was to research effects of P fertilizer and lime on growth of Trrifolium repens, Chamaecrista rotundifolia and Macroptilium atropur- pureum, to provide references for cultivation of the three plants. [Method] Pot experiments were conducted with Trrifolium repens, Charnaecrista rotundifolia and Macroptilium atropurpureum in 2010 in order to research effects of lime and P fer-tilizer mixture on growth of the plants in southern hilly acidic red soils. [Result] With lime amount fixed, application of P fertilizer would enhance plant height, total tiller number and dry matter. When P fertilizer was not applied, however, plant height of the three plants achieved the peak by lime at 1.4 g/kg which proved best for improvement of acidity of red soils. With P fertilizer at 200 mg/kg was applied, biomass of Trifolium repens and Macroptilium atropurpureum achieved the highest by lime at 2.1 g/kg, but total biomass of Chamaecrista rotundifolia was the highest by lime at 1.4 g/kg. [Conclusion] The research provides references for planting and production of Trifolium repens, Chamaecrista rotundifolia and Macroptilium atropur-pureum in southern hilly regions.
基金National Natural Science Foundation (30630014, 30570359)The grant of "Key Research Direction-KSCX2-YW-R-088" from Chinese Academy of Sciences~~
文摘In vertebrates, non-lens βγ-crystallins are widely expressed in various tissues and their functions are not well known. The molecular mechanisms of trefoil factors （TFFs）, which involved in mucosal healing and tumorigenesis, have remained elusive. βγ-CAT is a novel multifunctional protein complex of non-lens βγ-crystallin and trefoil factor from frog skin secretions. Here we report that βγ-CAT could induce sustained contraction of isolated rabbit aortic rings in dosage （2-35nmol/L） and endothelium dependent manners （P〈0.01 ）. In addition, in situ immunofluorescence indicated that positive TNF-α signals were mainly detected at the endothelial cell layer of βγ-CAT （25nmol/L） treated rings. Furthermore, βγ-CAT induced primary cultured rabbit thoracic aortic endothelial cells （RAECs） rapidly to release TNF-α. After βγ-CAT （25nmol/L） treated for 10 and 30min, the levels of the endothelial cells released TNF-ct were 34.17±5.10 pg/mL and 98.01±4.67 pg/mL （P〈0.01）, respectively. In conclusion, βγ-CAT could induce sustained contraction of isolated aortic rings, and the contractile effect might be partially explained by the release of TNF-α. These findings will give new insight into understanding the functions and physiological roles of non-lens βγ-crystallins and trefoil factors.
基金Supported by Doctor Foundation of Shanghai Institute of Technology~~
文摘[Objective] The aim was to study response of chlorophyll fluorescence parameters in Trifolium repens L.leaves to water stress from perspective of physiology.[Method] With T.repens cultivar ＂Haifa＂ as tested material,three soil water content levels were set to culture plants,including 75% （no stress,CK）,50% （moderate stress,LD） and 25% （severe stress,HD）,the effects of water stress on chlorophyll fluorescence parameters were determined.[Result] The results showed that the chlorophyll fluorescence parameters changed a little when the relative soil moisture content was 75% and 50%,while the chlorophyll fluorescence parameters such as conversion efficiency of primary light energy （Fv/Fm） of PS Ⅱ,the photochemical quenching coefficient （qP） and the quantum yield of PS Ⅱ electron transport （φPS Ⅱ） decreased respectively when the relative soil moisture content was 25%.[Conclusion] When T.repens in severe drought conditions,the physiological functions were damaged,showing symptoms of drought injury.
文摘Two new triterpenoid saponins, monepaloside K (1) and monepaloside L (2), together with a known saponin, mazusaponin I (3), were isolated from the water-soluble part of the whole plant of Marina nepalensis var. alba Hand.-Mazz. The structures of monepalosides K and L were determined to be 3-O-α-L-arabinopyranosyl-(1-3)-α-D-xylopyranosyl siaresinolic acid (1) and 3-O-β-D-glucopyranosyl-( 1-3)-β-L-arabinopyranosy1 siaresinolic acid 28-O-β-D-glucopyranosyl-(1-6)-β-D-glucopyranoside (2) respectively, on the basis of chemical and spectroscopic evidence.
基金Supported by the Program from Ludong University(043312)~~
文摘[Objective]The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province.[ Method] The root tip of A. macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, dissociated and stained for preparing the glass slide to reveal the chromosome number via the microscopic examination; the sparse cells with good chromosome morphology were photographed under microscope. [ Result ]Allium macrostemon Bunge in Laizhou City introduced in this study was tdploid; its somatic chromosome number was 24 and karyotype formula was K（2n） =3x=24m（2SAT） ＋ 1Bs, thus the karyotype belongs to 1A type. One of the chromosome No. 3 contained satellite, and chromosome deletion may be existed in one of the chromosome No. 5. In addition, B chromosome was observed in some cells.[ Conclusion] This introduction of triploid A. macrostemon found in China was the first time.
基金Supported by National Key Technology R & D Program(2006BAD16B07) Fund of Science and Technology in Guizhou Province ([2008]2074)~~
文摘[Objective] The study aimed to explore the degradation law and trend of artificial grassland. [Method] Taking the ryegrass （Lolium perenne） - white clover （ Trifolium repens） artificial grassland in Maiping Township, Guizhou Province as the research object, the grassland vegetation of 40 quadrate from different areas （area around the sheep shed, hilltop, hillside, flatland at the foot of the hill） were analyzed by comparing the dominance and richness index. [ Result] Degradation of different degrees appeared in various areas of this artificial grassland. To be specific, the degradation of grassland around the sheep shed was not serious for less consumption by the sheep; however, the areas on the hilltop and hillside degenerated to the grassland types of dallis grass （ Paspalum dilatatum Poir. ） -Indian lovegrass （ Eragrostis pilosa） and dallis grass - cogongrass [ Imperata cylindrica （ Linn. ） Beauv. ], respectively, and the area at the foot of the hill degenerated to the grassland type dominated by garland chrysanthetnum （ Chrysanthemum coronarium L. ） and knotgrass （ Paspalum distichum L. ）. [ Conclusion ] This study provided a basis for grassland improvement as well as the efficient and sustainable utilization of grazing-type artificial grassland in South China.
基金Supported by Guizhou Program for Agricultural Development([2009]3067)Guizhou Science and Technology Foundation([2012]2199)~~
文摘[Objective] The aim was to select and identify inorganic phosphorus-dissolving bacteria in rhizosphere of Trifolium repens,and to research related effects of growth promotion.[Method] Phosphate solubilizing bacteria in rhizosphere of Trifolium repens in Guizhou Province were separated and selected to determine its ability of phosphate solubilizing and IAA secretion,and growth promotion effect.[Result] Phosphate solubilizing bacteria were multiple and varied in rhizosphere of Trifolium repens;solubilizing quantities of phosphorus from calcium phosphate by RW2,RW6 and RW18 were 337.39,447.45 and 462.03 μg/ml;solubilizing quantity was none of linear relation with organic acid;RW2 proved the one secreted most IAA at 12.69 μg/ml,followed by RW18 at 12.34 μg/ml;the three strains were all of Leclercia sp.,but were of none pathogenicity for seedlings of Trifolium repens,instead,a kind of growth promotion was found.Detailedly,RW18 was most effective in growth promotion for Trifolium repens and plant height,root length and weight,and aboveground biomass increased by 110.98%,9.17%,46.17% and 2% respectively.Hence,RW18 is of great potential.[Conclusion] The research provides scientific references for developing phosphate solubilizing bacteria and compound inoculation and develops a novel way to relieve barren soils,save phosphorus resource,reduce environment pollution,and guarantee sustainable development of agriculture and animal husbandry.
文摘Chouioia cunea Yang is a natural enemy for many kinds of crop and forest pests, particularly for Hyphantria cunea Drary, which is an international quarantine pest. The experiment of rearing Chouioia cunea with Tussah (Silkworm) pupa were carried out by 搕hree-cut method?and inoculating method. The results showed that three-cut method is effective way for breed-ing Chouioia cunea, with a parasitical rate of 95%. The biological characteristics and the life cycle of Chouioia cunea were ob-served and described and more hosts of Chouioia cunea were found. 揟hree-cut method?as a new technique of rearing Chouioia cunea has been put into practice.
文摘A number of disorders have been described to cause protein losing enteropathy (PLE) in children. Primary intestinal lymphangiectasia (PIL) is one mechanism leading to PLE. Few syndromes are associated with PIL; Hennekam syndrome (HS) is one of them. The principal treatment for PIL is a high protein, low fat diet with medium chain triglycerides supplementation. Supportive therapy includes albumin infusion. Few publications have supported the use of octreotide to diminish protein loss and minimize hypoalbuminemia seen in PIL. There are no publications on the treatment of PIL with octreotide in patients with HS. We report two children with HS and PLE in which we used octreotide to decrease intestinal protein loss. In one patient, octreotide increased serum albumin to an acceptable level without further need for albumin infusions. The other patient responded more dramatically with near normal serum albumin levels and cessation of albumin infusions. In achieving a good response to octreotide in both patients, we add to the publications supporting the use of octreotide in PIL and suggest that octreotide should be tried in patients with PIL secondary to HS. To the best of our knowledge, this is the first case report on the use of octreotide in HS-associated PIL.
基金This project was supported by the Major State Basic Research Program of China （2005CB 120806）, National Natural Science Foundation of China for Distinguished Young Scholars （30525026） and the State Transgenic Plant Project （JY04-A-01）
文摘Heterotrimeric G proteins are known to function as messengers in numerous signal transduction pathways.The nullmutation of RGA(rice heterotrimeric G protein α subunit),which encodes the α subunit of heterotrimeric G proteinin rice,causes severe dwarfism and reduced responsiveness to gibberellic acid in rice.However,less is known aboutheterotrimeric G protein in brassinosteroid(BR)signaling,one of the well-understood phytohormone pathways.In thepresent study,we used root elongation inhibition assay,lamina inclination assay and coleoptile elongation analysis todemonstrated reduced sensitivity of dl mutant plants(caused by the null mutation of RGA)to 24-epibrassinolide(24-epiBL),which belongs to brassinosteroids and plays a wide variety of roles in plant growth and development.Moreover,RGA transcript level was decreased in 24-epiBL-treated seedlings in a dose-dependent manner.Our results show thatRGA is involved in rice brassinosteroid response,which may be beneficial to elucidate the molecular mechanisms of Gprotein signaling and provide a novel perspective to understand BR signaling in higher plants.
基金Supported by The National Natural Science Foundation of China, No. 30672564, No. 30472112 and No. 30070904
文摘AIM： To investigate the expression and activity of leukotriene C4 （LTC4） synthesis enzymes and their underlying relationship with cysteinyl leukotriene （cys-LT） generation in a rat fulminant hepatic failure （FHF） model induced by D-galactosamine/lipopolysaccharide （D-GaIN/ LPS）. METHODS： Rats were treated with D-GaIN （300 mg/kg） plus LPS （0.1 mg/kg） for 1, 3, 6, and 12 h. Enzyme immunoassay was used to determine the hepatic cys-LT content. Reverse transcription-polymerase chain reaction （RT-PCR）, Western blot or immunohistochemical assay were employed to assess the expression or location of LTC4 synthesis enzymes, which belong to membrane associated proteins in eicosanoid and glutathione （MAPEG） metabolism superfamily. Activity of LTC4 synthesis enzymes was evaluated by determination of the products of LTA4 after incubation with liver microsomes using high performance liquid chromatography （HPLC）. RESULTS： Livers were injured after treatment with D-GaIN/LPS, accompanied by cys-LT accumulation at the prophase of liver injury. Both LTC4 synthase （LTC4S） and microsomal glutathione-S-transferase （mGST） 2 were expressed in the rat liver, while the latter was specifically located in hepatocytes. Their mRNA and protein expressions were up-regulated at an earlier phase after treatment with D-GaIN/LPS. Meantime, a higher activity of LTC4 synthesis enzymes was detected, although theactivity of LTC4S played the main role in this case. CONCLUSION： The expression and activity of both LTC4S and mGST2 are up regulated in a rat FHF model, which are, at least, partly responsible for cys-LT hepatic accumulation.
