To investigate the effect of doxorubicin(DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR)Ca 2+ transport proteins and the mechanism of taurine(Tau) protecting cardiac muscle cells, 9 rabbits...To investigate the effect of doxorubicin(DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR)Ca 2+ transport proteins and the mechanism of taurine(Tau) protecting cardiac muscle cells, 9 rabbits were injected with DOX , 8 rabbits with DOX and Tau, and 9 rabbits with normal saline. Cardiac function , concentration of calcium in cardiomyocytes (Myo[Ca 2+ ] \%i\%), activity of SR Ca 2+ ATPase(SERCA2a), level of SERCA2a mRNA and Ca 2+ released channels(RYR2)mRNA were detected. The left ventricle tissues were observed by electron microscopy. The results showed that cardiac index, left ventricular systolic pressure, activity of SR Ca 2+ ATPase and level of SERCA2a mRNA decreased , while Myo[Ca 2+ ] \%i\% increased in DOX treated rabbits. DOX could not affect the level of RYR2 mRNA. Tau intervention could alleviate the increase of left ventricular diastolic pressure, Myo[Ca 2+ ] \%i\% and the decrease of SERCA2a mRNA induced by doxorubicin. The results suggested that downregulation of SERCA2a gene expression was an important mechanism of DOX induced cardiomyopathy and that Tau could partially improve the heart function by reducing calcium overload and alleviating downregulation of SERCA2a mRNA.展开更多
Objective： To observe the clinical efficacy of needling thirteen ghost acupoints for children with autism spectrum disorder. Methods： A total of 90 cases with autism spectrum disorder （ASD） aged between 2 and 6 ye...Objective： To observe the clinical efficacy of needling thirteen ghost acupoints for children with autism spectrum disorder. Methods： A total of 90 cases with autism spectrum disorder （ASD） aged between 2 and 6 years were randomly allocated into 2 groups by random number table. The control group （n=45） received routine rehabilitative training, and the treatment group （n=45） received acupuncture at thirteen ghost acupoints plus routine rehabilitative training. The Beijing Gesell developmental （Gesell） scale and autism behavior checklist （ABC） were used to assess the intellectual, language and behavior development before and 3 months after the treatment. Results： After the treatment, the total effective rate in the treatment group was 82.2%, versus 55.6% in the control group, showing a statistical significance （P〈0.05）. As for the scores of social, emotional and language in Gesell scale, there were significant intra-group differences in the treatment group （all P〈0.05）, and all the five subscales in the Gesell scale in the treatment group were significantly better than those in the control group （all P〈0.05）. As for the scores of ABC, there were significant intra-group differences in the treatment group （P〈0.05）, and the scores in the treatment group were significantly better than those in the control group （all P〈0.05）. Conclusion： Rehabilitation training plus acupuncture at thirteen ghost acupoints can significantly improve the intellectual, language and abnormal behavior in autism spectrum disorder children.展开更多
文摘To investigate the effect of doxorubicin(DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR)Ca 2+ transport proteins and the mechanism of taurine(Tau) protecting cardiac muscle cells, 9 rabbits were injected with DOX , 8 rabbits with DOX and Tau, and 9 rabbits with normal saline. Cardiac function , concentration of calcium in cardiomyocytes (Myo[Ca 2+ ] \%i\%), activity of SR Ca 2+ ATPase(SERCA2a), level of SERCA2a mRNA and Ca 2+ released channels(RYR2)mRNA were detected. The left ventricle tissues were observed by electron microscopy. The results showed that cardiac index, left ventricular systolic pressure, activity of SR Ca 2+ ATPase and level of SERCA2a mRNA decreased , while Myo[Ca 2+ ] \%i\% increased in DOX treated rabbits. DOX could not affect the level of RYR2 mRNA. Tau intervention could alleviate the increase of left ventricular diastolic pressure, Myo[Ca 2+ ] \%i\% and the decrease of SERCA2a mRNA induced by doxorubicin. The results suggested that downregulation of SERCA2a gene expression was an important mechanism of DOX induced cardiomyopathy and that Tau could partially improve the heart function by reducing calcium overload and alleviating downregulation of SERCA2a mRNA.
基金supported by Medical Science and Technology Key Project of Foshan Science and Technology Bureau, No.20151021020043~~
文摘Objective： To observe the clinical efficacy of needling thirteen ghost acupoints for children with autism spectrum disorder. Methods： A total of 90 cases with autism spectrum disorder （ASD） aged between 2 and 6 years were randomly allocated into 2 groups by random number table. The control group （n=45） received routine rehabilitative training, and the treatment group （n=45） received acupuncture at thirteen ghost acupoints plus routine rehabilitative training. The Beijing Gesell developmental （Gesell） scale and autism behavior checklist （ABC） were used to assess the intellectual, language and behavior development before and 3 months after the treatment. Results： After the treatment, the total effective rate in the treatment group was 82.2%, versus 55.6% in the control group, showing a statistical significance （P〈0.05）. As for the scores of social, emotional and language in Gesell scale, there were significant intra-group differences in the treatment group （all P〈0.05）, and all the five subscales in the Gesell scale in the treatment group were significantly better than those in the control group （all P〈0.05）. As for the scores of ABC, there were significant intra-group differences in the treatment group （P〈0.05）, and the scores in the treatment group were significantly better than those in the control group （all P〈0.05）. Conclusion： Rehabilitation training plus acupuncture at thirteen ghost acupoints can significantly improve the intellectual, language and abnormal behavior in autism spectrum disorder children.
