电针“三阴穴”对慢性非细菌性前列腺炎大鼠免疫功能的影响

目的探讨电针"三阴穴"对慢性非细菌性前列腺炎大鼠模型的免疫调节机制。方法将48只雄性SD大鼠随机分为空白组、模型组、药物组、电针组,皮内注射同种异体雄性SD大鼠前列腺蛋白提纯液,辅以双重免疫佐剂复制自身免疫性慢性非细菌性前列腺炎大鼠模型。造模后电针组、药物组分别给予电针"三阴穴"、舍尼通灌胃治疗15 d。观察各组动物前列腺组织湿重及系数、形态结构;放免法检测前列腺组织中抗体Ig G、局部组织细胞因子白介素(IL)-2、IL-8的变化。结果造模后模型组前列腺重量及系数与空白组相比显著增高(P<0.05),病理观察显示,模型组动物前列腺组织形态及结构明显受损。与空白组比较,模型组大鼠前列腺组织匀浆中Ig G含量明显增高(P<0.05),IL-2、IL-8高表达(P<0.05,P<0.01)。治疗后,电针组动物腺体湿重及系数均明显下降(P<0.05,P<0.01),病理变化明显减轻;局部前列腺组织Ig G含量明显降低(P<0.05),IL-2、IL-8表达显著减轻(P<0.05,P<0.01)。结论电针"三阴穴"能够减少局部抗体Ig G的分泌,调节局部细胞因子IL-2、IL-8的表达,减轻组织局部免疫亢进,抑制前列腺组织形态结构的损伤,从而起到治疗慢性非细菌性前列腺炎的作用。

电针“三阴穴”对慢性非细菌性前列腺炎大鼠免疫功能的影响

目的观察电针"三阴穴"对慢性非细菌性前列腺炎大鼠模型免疫功能的影响,并探讨其作用机制。方法将48只雄性SD大鼠随机分为空白组、模型组、药物组、电针组。皮内注射同种异体雄性SD大鼠前列腺蛋白提纯液,辅以双重免疫佐剂复制自身免疫性慢性非细菌性前列腺炎大鼠模型。造模后电针组、药物组分别给予电针"三阴穴"、舍尼通灌胃治疗15 d。观察各组动物前列腺组织湿重及指数、形态结构,用放免法检测前列腺组织中抗体IgG含量,局部组织细胞因子白细胞介素(IL)-2、IL-8的变化。结果造模后模型组前列腺组织湿重及指数与空白组比较显著增高(P<0.05),病理观察显示,模型组动物前列腺组织形态及结构明显受损;与空白组比较,模型组大鼠前列腺组织匀浆中IgG含量明显增高(P<0.05),局部细胞因子IL-2、IL-8高表达(P<0.05,P<0.01)。治疗后,电针组前列腺湿重及指数均明显下降(P<0.05,P<0.01),病理变化明显减轻;局部前列腺组织IgG含量明显降低(P<0.05),局部细胞因子IL-2、IL-8表达显著下降(P<0.05,P<0.01)。结论电针"三阴穴"能够减少非细菌性前列腺炎大鼠局部抗体IgG的分泌,调节局部细胞因子IL-2、IL-8的表达,减轻组织局部免疫亢进,抑制前列腺组织形态结构的损伤,从而起到治疗慢性非细菌性前列腺炎的作用。

Effect of moxibustion at Sānyīnjiāo (三阴交 SP 6) on uterine contraction pain in labor: a randomized controlled trial

Objective To investigate the effect of moxibustion at Sanyinjiao （三阴交 SP 6） for uterine contraction pain in labor, and evaluate the safety of the parturient and newborn. Methods One hundred and seventy-four cases of singleton pregnancy and cephalic presentation primipara were single blinded and randomly divided into three groups： observation group （59 cases）, placebo treated group （57 cases） and blank group （58 cases）.The observation group was treated with moxibustion at Sanyinjiao （三阴交 SP 6） for 30 min when the uterus cervix openning at 3 cm,the placebo treated group was treated with moxibustion at non-aeupoint for 30 min and the blank group was treated with routine labor nursing, the uterine contraction pain and the safety of the mother and infant were compared among three groups. Results （1） The uterine contraction pain was tested by Visual Analogue Scale （VAS）： the scores of VAS in the observation group were obviously decreased after 15 min and 30 min of moxibustion （both P〈0.05）, there were no obvious changes of the VAS scores in placebo treated group and the blank group, the scores of VAS in observation group decreased much more obviously than those in the other two groups （all P〈0.05）; （2）Midwife rating of the uterine contraction pain： after 30 min of moxibustion, the effective rate of labor analgesia was 69.5%（41/59） in observation group, which was higher than that of 45.6% （26/57） in placebo treated group and 43.1% （25/58） in blank group, with significant differences between them （both P〈0.05）; @The postpartum hemorrhage amount of the observation group was obviously lower than those of placebo treated group and blank group （both P〈0.05）; （4）The Apgar score of newborn was higher in observation group and placebo treated group than that of blank group （both P〈0.05）. Conclusion Moxibustion at Sanyinjiao （三阴交 SP 6） can relieve the uterine contraction pain, and has no side effect to mother and infant, it is one of the safe, effective and simple non-drug analgesia methods.

Application of compatibility of Sānyīnjiāo (三阴交 SP 6)

SanyTnjiao （三阴交 SP 6） is not only a shu-point of the Spleen Meridian of Foot- Taiyin, but also a crossing point of three ）/in meridians of foot, which is widely selected in clinic to eliminate evil and strengthen the origin. Its clinical efficacy is obvious with appropriate prescription. With three cases of dysuria, bulbar paralysis and dysmenorrhea, action characteristic that SP 6 can regulate qi-blood of three meridians and three zang organs as well as treating disease from the primary cause and inspiring qi-blood of three meridians is explained.

Acupoints selection pattern in acupuncture-moxibustion treatment of perimenopausal syndrome from 2007 to 2016

Objective： To discuss the acupoints selection pattern in acupuncture-moxibustion treatment of perimenopausal syndrome（PMS） from 2007 to 2016. Methods： Clinical literatures related to PMS treated with acupuncture-moxibustion published from 2007 to 2016 were collected from Chinese Biomedical Literature Database（CBM）, Chongqing VIP Database（CQVIP）, China National Knowledge Infrastructure（CNKI）, and Wanfang Academic Journal Full-text Database（Wanfang）. The retrieved data underwent descriptive analysis, cluster analysis and association pattern analysis to determine the acupoints selection principle in acupuncture-moxibustion treatment of PMS. Results： The top five acupoints used in acupuncture-moxibustion treatment of PMS were Sanyinjiao（SP 6）, Shenshu（BL 23）, Guanyuan（CV 4）, Baihui（GV 20）, and Shenmen（HT 7）. The leading 4 meridians were Bladder Meridian, Conception Vessel, Spleen Meridian, and Governor Vessel. The clustering analysis showed that the 5 core acupoint groups were：（1） Sanyinjiao（SP 6）;（2） Shenshu（BL 23） and Guanyuan（CV 4）;（3） Baihui（GV 20）, Shenmen（HT 7）, Zusanli（ST 36）, Ganshu（BL 18） and Taichong（LR 3）;（4） Taixi（KI 3）, Pishu（BL 20）, Xinshu（BL 15）, Qihai（CV 6） and Neiguan（PC 6）;（5） Sishencong（EX-HN 1）, Zhongwan（CV 12）, Hegu（LI 4）, Yintang（GV 29）, Fengchi（GB 20）, Zhongji（CV 3） and Feishu（BL 13）. The three most significant acupoints were Sanyinjiao（SP 6）, Shenshu（BL 23） and Guanyuan（CV 4）. Acupoint groups based on syndrome differentiation included：（1） Hegu（LI 4）, Zhongwan（CV 12） and Sishencong（EX-HN 1）;（2） Feishu（BL 13）, Zhongji（CV 3）, Fengchi（GB 20） and Yintang（GV 29）;（3） Xinshu（BL 15）, Pishu（BL 20）, Qihai（CV 6）, Neiguan（PC 6） and Taixi（KI 3）;（4） Ganshu（BL 18）, Zusanli（ST 36）, Shenmen（HT 7）, Taichong（LR 3） and Baihui（GV 20）. The analysis of association pattern elaborated that Shenshu（BL 23） and Sanyinjiao（SP 6） won the highest support rate in the paired groups; Ganshu（BL 18）, Shenshu（BL 23） and Sanyinjiao（SP 6） had the highest support rate among the acupoint groups. Conclusion： The data mining results of acupuncture-moxibustion treatment of PMS substantially conform to the general principle in traditional acupuncture-moxibustion theories, able to reflect the acupoints selection and grouping pattern and provide references for acupuncture-moxibustion treatment of PMS.

Clinical Observation on Treating Menopause Syndrome with Acupuncture Therapy

Objective： To study the clinical effect and the endocnne mechanism ot acupuncture therapy on menopause syndrome. Method： There were 47 cases with menopause syndrome, which were divided into two groups, 32 cases in the acupuncture group, treated by acupuncture with main acupoins of Shenshu （BL 23）, Zusanli （ST 36） and Sanyinjiao （SP 6）, and 15 cases in the control group, treated by orally taking the Oryzanol. The Kupperman index of the two groups was observed before and after treatment for evaluating the therapeutic effect. The changes of blood-serum E2, T, FSH, LH of the 25 cases in the acupuncture group were tested by radioimmunoassay before and after the treatment. Results： The acupuncture therapy can relieve the clinical symptoms of menopause syndrome, and its therapeutic effect was better than Oryzanol. After being treated by acupuncture therapy, the content of blood-serum E2 obviously increased, while the contents of LH and T declined, and the FSH also had a tendency to lower, but it was not statistically significant. Conclusion： The therapeutic effect of acupuncture therapy for menopause is confirmed. The acupuncture therapy can improve the function of hypophysial-gonadal axis of the patient with menopause syndrome, which may be the main mechanism of the acupuncture therapy for treating menopause syndrome.

Treatment of 90 Cases of Simple Obesity with Intermuscular Needling Method

Summary： The patient takes a supine position, 10-20 needles were inserted thickly with intermuscular needling method in the two rounds, whose centers were both Tianshu （ST 25） and radii were 2 cun. Then, the electric acupuncture apparatus was connected. Meanwhile, bilateral Yanglingquan （GB 34）, Sanyinjiao （SP＇6）, Taichong （LR 3）, Ouchi （LI 11） and Zhigou （TE 6） were needled. Total 60 cases of obesity were treated with these methods, and 30 cases with conservative acupuncture as a control. The treatment results showed that the effective rates were 91.7% and 70.0%, respectively in the two groups.

Treatment of 30 Cases of Migraine by Mind-regulating Acupuncture

Objective： To observe the clinical effects of needling acupoints Shenting（GV 24）, Neiguan（PC 6） and Sanyinjiao（SP 6） in the treatment of migraine. Methods： Thirty cases of Germen patients with migraine were treated mainly by Shenting（GV 24）, bilateral Neiguan（PC 6） and Sanyinjiao（SP 6）, as well as bilateral Hegu（LI 4） and Taichong（LR 3）, Touwei（ST 8）, Yangbai（GB 14）, Cuanzhu（BL 2）, Sizhukong（TE 23）, Shuaigu（GB 8） and Sibai（ST 2） in the affected side of head, auricular Sympathetic （MA-AH 7）, Er Shenmen （MA-TF 1）, Heart （MA- IC） and Endocrine （MA-IC 3）; another 28 cases were treated by routine acupuncture. The clinical results and immediate pain relieving effects were observed. Results. The total effective rate was 90.0% in treatment group and 75.0% in control group, X^2= 4.57, P 〈 0.05; the 24 hour pain-relieving effects were obviously better in treatment group than that in control group, X^2= 10.11, P〈 0.01. Conclusion： Needling Shenting（GV 24）, Neiguan （PC 6） and San yinjiao（SP 6） has better effects than routine acupuncture in the treatment of migraine.

Tuina Treatment for 37 Cases of Postoperative Urinary Retention

Objective： To observe the effect of postoperative urinary retention treated with tuina therapy. Methods： Tuina therapy was applied on 37 cases of postoperative urinary retention; Qihai （CV 6）, Guanyuan （CV 4）, Zhongji （CV 3） and bilateral Sanyinjiao （SP 6） were selected. Results： Thirty-six cases were cured and 1 case was not surveyed after 1 to 3 treatments. Conclusion： Point tuina is an effective method in treating postoperative urinary retention.

Study on the Treatment of Acute Cerebral Infarction by Xuanzhong(GB 39)Toward Sanyinjiao(SP 6)Acupuncture as Main Therapy

Objective： To investigate the effects of acupuncture therapy on acute cerebral infarction. Methods： Eighty patients with acute cerebral infarction were randomly divided into a treatment group of 40 cases and a control group of 40 cases. Xuanzhong（GB 39）-throughSanyinjiao（SP 6） acupuncture was performed as a main treatment. The curative effects were compared between the two groups and the sizes of cerebral infarct, between pretreatment and posttreatment after one course of treatment. Results. The total recovery rate was 88.5% in the treatment group and 57.5% in the control group after one course of treatment. There was a significant difference between the two（P〈 0.05）. The rate of change in the infarct for the better was significantly higher in the treatment group than in the control group. There was also a significant difference（P〈 0.05）, Conclusion： This treatment is an effective method for lowering the rate of apoplectic disability and raising the cure rate,

Effect of Anrou-pressing and kneading Hegu(LI 4)and Sanyinjiao(SP 6)on uterine inertia during painless parturition

Objective:To observe the effect of Anrou-pressing and kneading Hegu(LI 4)and Sanyinjiao(SP 6)on uterine inertia during painless parturition.Methods:A total of 100 cases of patients with uterine inertia during painless parturition were randomized into an acupoint group and a medicine group by the SPSS programming,with 50 cases in each group.Patients in the acupoint group received the treatment of Anrou-pressing and kneading bilateral Hegu(LI 4)and Sanyinjiao(SP 6),while patients in the medicine group received intravenous oxytocin.The labor time and maternal-child safety were evaluated,and the labor stage was compared.Results:The latent and active phases in the 1st labor stage and the 2nd labor stage in the acupoint group were substantially shorter than those in the medicine group,and the between-group comparisons showed statistical significance(all P<0.05).The between-group comparison of the 3rd labor stage showed no statistical significance(P>0.05).The postpartum hemorrhage amount in both groups was within the safe range,and the between-group comparison showed no statistical significance(P>0.05).The newborn 1 min Apgar score was ranged 8-10 points,and the between-group comparison showed no statistical significance(P>0.05).The between-group comparison of the labor stage efficacy showed statistical significance(P<0.01),with a better progression in the acupoint group.Conclusion:Anrou-pressing and kneading Hegu(LI 4)and Sanyinjiao(SP 6)is both effective and safe for uterine inertia during painless parturition.

Clinical studies on different acupuncture time for primary dysmenorrhea

Objective： To seek the optimal acupuncture time for primary dysmenorrhea and provide clinical basis for optimal acupuncture treatment protocol. Methods： A total of 90 eligible cases were randomly allocated into three groups, 30 cases in each group. Points Guanyuan（CV 4）, bilateral Zusanli（ST 36） and Sanyinjiao（SP 6） were selected for patients in all three groups, with a different treatment duration： 15 min in group A, 30 min in group B and 45 min in group C. Then the clinical efficacy in each group was evaluated by pain symptom scoring. Results： As for the pain symptom scores, there were statistically significant intra-group differences between before and after treatment in three groups（all P〈0.05）; coupled with statistically significant inter-group differences between group B and the other two groups（both P〈0.05）. As for clinical efficacy, there were statistical differences between group B and the other two groups（both P〈0.05）, indicating that 30 min of acupuncture is the optimal duration in the treatment of dysmenorrhea. Conclusion： With the same needling manipulation, 30 min of acupuncture treatment achieves a better efficacy for primary dysmenorrhea.

Clinical Observation on Carpal Joint Sprain by Puncturing Juliao （GB 29）

Objective： To observe the effect of puncturing Juliao （GB 29） in treating carpal joint sprain. Methods： 99 cases were randomly divided into 3 groups, treatment, control 1 and control 2 groups, 33 cases for each group. Cases in treatment group were treated by acupuncture on Juliao （GB 29）; patients in control 1 group were treated by acupuncture at Sanyinjiao （SP 6）, and patients in control 2 group were treated by local application. The clinical effect was compared among three groups after the treatment. Results： The effective rate in the treatment group is 96.9%, which is over and above 84.8% in control 1 group and 75.8% in control 2 group （both P〈0.05）. Conclusion： Acupuncture at Juliao （GB 29） has a precise curative effect in treating sprain of carpal joint. It is more effective than acupuncture at Sanyinjiao （SP 6） and local application.

Treatment of 68 Cases of Epigastric Pain with Acupucnture

Acute and chronic gastritis,gastric and duodenalglobular ulcer,and antral gastritis are commonlyencountered diseases of digestive system,andepigastric pain is their main symptom.The authortreated 68 cases of epigastric pain with acupuncture,now it is reported as follows.