The tunnel subjected to strike-slip fault dislocation exhibits severe and catastrophic damage.The existing analysis models frequently assume uniform fault displacement and fixed fault plane position.In contrast,post-e...The tunnel subjected to strike-slip fault dislocation exhibits severe and catastrophic damage.The existing analysis models frequently assume uniform fault displacement and fixed fault plane position.In contrast,post-earthquake observations indicate that the displacement near the fault zone is typically nonuniform,and the fault plane position is uncertain.In this study,we first established a series of improved governing equations to analyze the mechanical response of tunnels under strike-slip fault dislocation.The proposed methodology incorporated key factors such as nonuniform fault displacement and uncertain fault plane position into the governing equations,thereby significantly enhancing the applicability range and accuracy of the model.In contrast to previous analytical models,the maximum computational error has decreased from 57.1%to 1.1%.Subsequently,we conducted a rigorous validation of the proposed methodology by undertaking a comparative analysis with a 3D finite element numerical model,and the results from both approaches exhibited a high degree of qualitative and quantitative agreement with a maximum error of 9.9%.Finally,the proposed methodology was utilized to perform a parametric analysis to explore the effects of various parameters,such as fault displacement,fault zone width,fault zone strength,the ratio of maximum fault displacement of the hanging wall to the footwall,and fault plane position,on the response of tunnels subjected to strike-slip fault dislocation.The findings indicate a progressive increase in the peak internal forces of the tunnel with the rise in fault displacement and fault zone strength.Conversely,an augmentation in fault zone width is found to contribute to a decrease in the peak internal forces.For example,for a fault zone width of 10 m,the peak values of bending moment,shear force,and axial force are approximately 46.9%,102.4%,and 28.7% higher,respectively,compared to those observed for a fault zone width of 50 m.Furthermore,the position of the peak internal forces is influenced by variations in the ratio of maximum fault displacement of the hanging wall to footwall and the fault plane location,while the peak values of shear force and axial force always align with the fault plane.The maximum peak internal forces are observed when the footwall exclusively bears the entirety of the fault displacement,corresponding to a ratio of 0:1.The peak values of bending moment,shear force,and axial force for the ratio of 0:1 amount to approximately 123.8%,148.6%,and 111.1% of those for the ratio of 0.5:0.5,respectively.展开更多
Aimed at the problem of stochastic routings for reprocessing operations and highly variable processing times,an open queuing network is utilized to model a typical reprocessing system.In the model,each server is subje...Aimed at the problem of stochastic routings for reprocessing operations and highly variable processing times,an open queuing network is utilized to model a typical reprocessing system.In the model,each server is subject to breakdown and has a finite buffer capacity,while repair times,breakdown times and service time follow an exponential distribution.Based on the decomposition principle and the expansion methodology,an approximation analytical algorithm is proposed to calculate the mean reprocessing time,the throughput of each server and other parameters of the processing system.Then an approach to determining the quality of disassembled parts is suggested,on the basis of which the effect of parts quality on the performance of the reprocessing system is investigated.Numerical examples show that there is a negative correlation between quality of parts and their mean reprocessing time.Furthermore,marginal reprocessing time of the parts decrease with the drop in their quality.展开更多
Taking variability and uncertainty involved in performance prediction into account, in order to make the prediction reliable and meaningful, a distribution-based method is developed to predict future PSI. This method,...Taking variability and uncertainty involved in performance prediction into account, in order to make the prediction reliable and meaningful, a distribution-based method is developed to predict future PSI. This method, which is based on the AASHTO pavement performance model, treats predictor variables as random variables with certain probability distributions and obtains the distribution of future PSI through the method of Monte-Carlo simulation. A computer program PERFORM using Monte Carlo simulation is developed to implement the numerical computation. Simulation results based on pavement and traffic parameters show that traffic, surface layer material property, and initial pavement performance are the most significant factors affecting pavement performance. Once the distribution of future PSI is determined, statistics such as the mean and the variance of future PSI are readily available.展开更多
Pingxiang-dominant genic male sterile rice (PDGMSR) was the first dominant genic male sterile mutant identified in rice (Oryza sativa L.), and the corresponding dominant genic male sterile gene was designated as M...Pingxiang-dominant genic male sterile rice (PDGMSR) was the first dominant genic male sterile mutant identified in rice (Oryza sativa L.), and the corresponding dominant genic male sterile gene was designated as Ms-p. The fertility of PDGMSR can be restored by introduction of a dominant epistatic fertility restoring gene in some rice varieties. In the present study, E823, an indica inbred rice variety, restored the fertility of PDGMSR, and the genetic pattern was found to be consistent with a dominant epistatic model, therefore, the dominant epistatic fertility restorer gene was designated as Rfe. The F2 population from the cross of PDGMSR/E823 was developed to map gene Rfe. The F2 plants with the genotypes Ms-pMs-pRferfe or Ms-pms-pRferfe were used to construct a fertile pool, and the corresponding sterile plants with genotypes Ms-pMs-prferfe or Ms-pms-prferfe were used to con- struct a sterile pool. The fertility restoring gene Rfe was mapped to one side of the microsatellite markers RM311 and RM3152 on rice chromosome 10, with genetic distances of 7.9 cM and 3.6 cM, respectively. The microsatellite markers around the location of the Ms-p gene were used to finely map the Ms-p gene. The findings of this study indicated that the microsatellite markers RM171 and RM6745 flanked the Ms-p gene, and the distances were 0.3 cM and 3.0 cM, respectively. On the basis of the sequence of rice chromosome 10, the physical distance between the two markers is approximately 730 kb. These findings facilitates molecular marker-assisted selection (MAS) of genes Ms-p and Rfe in rice breeding programs, and cloning them in the future.展开更多
To reduce the high computational cost of the uncertainty analysis, a procedure is proposed for the aerodynamic optimization under uncertainties, in which the surrogate model is used to simplify the computation of the ...To reduce the high computational cost of the uncertainty analysis, a procedure is proposed for the aerodynamic optimization under uncertainties, in which the surrogate model is used to simplify the computation of the uncertainty analysis. The surrogate model is constructed by using the Latin Hypercube design and the Kriging model. The random parameters are used to account for the small manufacturing errors and the variations of operating conditions. Based on the surrogate model, an uncertainty analysis approach, called the Monte Carlo simulation, is used to compute the mean value and the variance of the predicated performance. The robust optimization for aerodynamic design is formulated, and solved by the genetic algorithm. And then, an airfoil optimization problem is used to test the proposed procedure. Results show that the optimal solutions obtained from the uncertainty-based optimization formulation are less sensitive to uncertainties. And the design constraints are still satisfied under the uncertainties.展开更多
To take into account the influence of uncetainties on the dynamic response of the vibro-acousitc structure, a hybrid modeling technique combining the finite element method(FE)and the statistic energy analysis(SEA)...To take into account the influence of uncetainties on the dynamic response of the vibro-acousitc structure, a hybrid modeling technique combining the finite element method(FE)and the statistic energy analysis(SEA) is proposed to analyze vibro-acoustics responses with uncertainties at middle frequencies. The mid-frequency dynamic response of the framework-plate structure with uncertainties is studied based on the hybrid FE-SEA method and the Monte Carlo(MC)simulation is performed so as to provide a benchmark comparison with the hybrid method. The energy response of the framework-plate structure matches well with the MC simulation results, which validates the effectiveness of the hybrid FE-SEA method considering both the complexity of the vibro-acoustic structure and the uncertainties in mid-frequency vibro-acousitc analysis. Based on the hybrid method, a vibroacoustic model of a construction machinery cab with random properties is established, and the excitations of the model are measured by experiments. The responses of the sound pressure level of the cab and the vibration power spectrum density of the front windscreen are calculated and compared with those of the experiment. At middle frequencies, the results have a good consistency with the tests and the prediction error is less than 3. 5dB.展开更多
Coastal sediment type map has been widely used in marine economic and engineering activities, but the traditional mapping methods had some limitations due to their intrinsic assumption or subjectivity. In this paper, ...Coastal sediment type map has been widely used in marine economic and engineering activities, but the traditional mapping methods had some limitations due to their intrinsic assumption or subjectivity. In this paper, a non-parametric indicator Kriging method has been proposed for generating coastal sediment map. The method can effectively avoid mapping subjectivity, has no special requirements for the sample data to meet second-order stationary or normal distribution, and can also provide useful information on the quantitative evaluation of mapping uncertainty. The application of the method in the southern sea area of Lianyungang showed that much more convincing mapping results could be obtained compared with the traditional methods such as IDW, Kriging and Voronoi diagram under the same condition, so the proposed method was applicable with great utilization value.展开更多
Aimed at the remanufacturing system, the effect of the uncertainty of returns' quality on bottleneck shifting is investigated. A novel definition of bottleneck station is presented and the probability of a station be...Aimed at the remanufacturing system, the effect of the uncertainty of returns' quality on bottleneck shifting is investigated. A novel definition of bottleneck station is presented and the probability of a station becoming a bottleneck is also given. By calculating the effective output, the effective operation time (EOT) and the ratio of EOT of each station, the system's current bottleneck of effective output time is determined. By calculating the probability coefficient of variation and index of bottleneck shifting, the quantitative performance of bottleneck shifting is obtained. Discrete event simulation and the experiment design method are adopted to simulate the system, in which the proportion of quality grading, repair rates and process routes are considered. The case study shows that the uncertainty of returns' quality greatly increases the probability of bottleneck shifting, and with the increase of the discrete degree of the returns' repair rate, the bottleneck shifting phenomenon is more obvious. Furthermore, bottleneck shifting is closely related to the process route of the dominating returns' quality grade.展开更多
Objective: To study the features of microsatellite alterations on chromosome 8 and their asso- ciation with clinicopathological characteristics of hepatocellular carcinoma (HCC). Methods: Ten highly- ...Objective: To study the features of microsatellite alterations on chromosome 8 and their asso- ciation with clinicopathological characteristics of hepatocellular carcinoma (HCC). Methods: Ten highly- polymorphic microsatellite markers on chromosome 8 were selected to be detected for loss of heterozygosity (LOH), microsatellite instability (MSI) and allelic imbalance (AI) in 56 HCC using MegaBACE 500 auto- matic DNA analysis system. Results: LOH was found in 37 of 56 HCC (66.1%) on at least 1 locus, and the top three loci were D8S261(53.5%), D8S1721(52.5%) and D8S1771(52.5%). LOH frequency on D8S277 was signi?cantly higher in cases positive for serum HBsAg than in those negative for HBsAg (P <0.01). Similarly, LOH on D8S261, D8S298 and D8S1733 occurred more frequently in patients with negative HB- sAg than those with positive HBsAg (P <0.01). LOH on D8S298 and D8S1771 was more frequent in those tumors larger than 3 cm in size (P <0.05 or P <0.01). LOH frequencies of D8S1721 were signi?cantly higher in the patients with absent or not intact tumor capsule than in those with intact tumor capsule (P <0.05). LOH on D8S298 and D8S1771 was more frequently detected in tumors with intrahepatic metastasis than in those without intrahepatic metastasis (P <0.01). MSI was found in 12.5% (7/56) cases, and AI was found in 19.6% (11/56), no correlation was found between MSI and AI and clinicopathological character- istics of HCC. Conclusion: Frequent microsatellite alterations on chromosome 8 existed in HCC. LOH, which represents tumor suppressor gene pathway, plays a more important role in hepatocarcinogenesis; MSI representing mismatch repair gene pathway ranks next. LOH at a particula locus is associated with the clinicopathological parameters of human HCC.展开更多
In acoustic logging-while-drilling (ALWD) finite difference in time domain (FDTD) simulations, large drill collar occupies, most of the fluid-filled borehole and divides the borehole fluid into two thin fluid colu...In acoustic logging-while-drilling (ALWD) finite difference in time domain (FDTD) simulations, large drill collar occupies, most of the fluid-filled borehole and divides the borehole fluid into two thin fluid columns (radius -27 mm). Fine grids and large computational models are required to model the thin fluid region between the tool and the formation. As a result, small time step and more iterations are needed, which increases the cumulative numerical error. Furthermore, due to high impedance contrast between the drill collar and fluid in the borehole (the difference is 〉30 times), the stability and efficiency of the perfectly matched layer (PML) scheme is critical to simulate complicated wave modes accurately. In this paper, we compared four different PML implementations in a staggered grid finite difference in time domain (FDTD) in the ALWD simulation, including field-splitting PML (SPML), multiaxial PML(M- PML), non-splitting PML (NPML), and complex frequency-shifted PML (CFS-PML). The comparison indicated that NPML and CFS-PML can absorb the guided wave reflection from the computational boundaries more efficiently than SPML and M-PML. For large simulation time, SPML, M-PML, and NPML are numerically unstable. However, the stability of M-PML can be improved further to some extent. Based on the analysis, we proposed that the CFS-PML method is used in FDTD to eliminate the numerical instability and to improve the efficiency of absorption in the PML layers for LWD modeling. The optimal values of CFS-PML parameters in the LWD simulation were investigated based on thousands of 3D simulations. For typical LWD cases, the best maximum value of the quadratic damping profile was obtained using one do. The optimal parameter space for the maximum value of the linear frequency-shifted factor (a0) and the scaling factor (β0) depended on the thickness of the PML layer. For typical formations, if the PML thickness is 10 grid points, the global error can be reduced to 〈1% using the optimal PML parameters, and the error will decrease as the PML thickness increases.展开更多
In cultivated rice ( Oryza sativa L.), F-1 pollen sterility is controlled by at least 6 loci of the F, pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-b(j)/S-b(j) and its nea...In cultivated rice ( Oryza sativa L.), F-1 pollen sterility is controlled by at least 6 loci of the F, pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-b(j)/S-b(j) and its near-isogenic line TIST2 carrying S-b(i)/S-b(i) were used to develop the mapping population. One hundred and fifty-eight microsatellite markers were selected to survey T65 and TISL2. RM13 on chromosome 5 was found to be polymorphic between them. Cosegregation indicated that RM13 was closely linked with locus S-b. Eleven RFLP markers were selected on the corresponding region from the genetic map of Rice Genome Research Program (RGP) of Japan to convert into sequence-tagged site (STS) markers. Amplicon length polymorphism (ALP) was carried out, but none of them was found to be polymorphic between T65 and TISL2. Then PCR-based RFLP (PBR) was done using six 4-nucleotide recognizing restriction endonucleases. Polymorphism was detected when PCR products of R830STS and R2213SSTS were digested with Taq I. Genetic analysis indicated that the distance between locus S-b and markers, R830STS, RM13 and R2213SSTS were 3.3 cM (centi-Morgan), 5.2 cM and 5.5 cM, respectively. These PCR-based markers could be directly used in marker-assisted selection. The technical system combining genetic mapping and PCR-based marker-assisted selection will facilitate the development of molecular breeding.展开更多
Aim The solvability condition for robust stabilization problem associated with a plant family P(s,δ) having parameter uncertainty δ was considered. Methods Using Youla parameterization of the stabilizers this pro...Aim The solvability condition for robust stabilization problem associated with a plant family P(s,δ) having parameter uncertainty δ was considered. Methods Using Youla parameterization of the stabilizers this problem was transformed into a strong stabilization problem associated with a related plant family G (s, δ). Results A necessary solvability condition was established in terms of the parity interlacing property of each element in G(s,δ). Another apparently necessary solvability condition is that every element in P(s,δ) must be stabilizable. Conclusion The two conditions will be compared with each other and it will be shown that every element in G(s,δ) possesses parity interlacing property if P(s,δ) is stabilizable.展开更多
[ Objective] The study was to investigate the agronomic traits and breeding characteristics of genic male sterile material Mian 7AB-4- 2 in Brassica napus. [ Method] The differences in agronomic traits and polymorphis...[ Objective] The study was to investigate the agronomic traits and breeding characteristics of genic male sterile material Mian 7AB-4- 2 in Brassica napus. [ Method] The differences in agronomic traits and polymorphisms in SSR markers, between the genic male sterile material Mian 7AB-4-2 in Brassica napus and its sisterly line Mian 7AB-4-1 were investigated by hybridization and molecular identification; and the percentage of sterile individuals of Mian 7AB-4-2 and of the hybrids with its sisterly line Mian 7AB-4-1 from test cross and back cross were also studled. [ Result] Mian 7AB-4-2 was not significantly different in agronomic traits from its sisterly line Mian 7AB-4-1 at 0.05 probability level. The per- centages of sterile individuals in the pollinated fertile Mian 7AB-4-2 plants were over 60%, and that in its sisterly line Mian 7AB-4-1 was about 25%. In test crosses with other nine sterile lines, Mian 7AB-4-1 kept the percentage of sterile individuals of sterile lines over 90%, and the percentage of sterile individuals from back cross over 80%. With regard to molecular markers, Mian 7AB-4-2 and its sisterly line Mian 7AB-4-1 were different in the band number from SSR primers a2 and E10. [ Conclusion] The results indicate that Mian 7AB-4-2 is helpful for rapeseed breeding, quickening the application of new materials in field breeding.展开更多
We propose a new method for robust adaptive backstepping control of nonlinear systems with parametric uncertainties and disturbances in the strict feedback form. The method is called dynamic surface control. Traditio...We propose a new method for robust adaptive backstepping control of nonlinear systems with parametric uncertainties and disturbances in the strict feedback form. The method is called dynamic surface control. Traditional backstepping algorithms require repeated differentiations of the modelled nonlinearities. The addition of n first order low pass filters allows the algorithm to be implemented without differentiating any model nonlinearities, thus ending the complexity arising due to the 'explosion of terms' that makes other methods difficult to implement in practice. The combined robust adaptive backstepping/first order filter system is proved to be semiglobally asymptotically stable for sufficiently fast filters by a singular perturbation approach. The simulation results demonstrate the feasibility and effectiveness of the controller designed by the method.展开更多
[Objective] This study was conducted to identify the thermo-sensitivity of the male sterile line (NYB) of Ningxia hot pepper. [Method] The pol en abortion rates of pepper male sterile line under artificial y control...[Objective] This study was conducted to identify the thermo-sensitivity of the male sterile line (NYB) of Ningxia hot pepper. [Method] The pol en abortion rates of pepper male sterile line under artificial y control ed temperature condition and nature temperature condition were measured and compared. [Result] The pol en abortion was the sign of NYB male sterility. The pol en abortion rate changed little under different temperature conditions, while pol en amount greatly reduced at low temperature, indicating that NYB was genetical y stable in field. [Conclusion] The re-sults can provide some theoretical references for the application of NYB pepper male sterile line in breeding work.展开更多
In order to study the stability of desert climate, the instability of climate in Minqin desert in response to global warming during 1961-2013 was analyzed by sliding standard deviation and a linear trend line. The res...In order to study the stability of desert climate, the instability of climate in Minqin desert in response to global warming during 1961-2013 was analyzed by sliding standard deviation and a linear trend line. The results show that the instabili- ty of average temperature in January and April increased, and the isothermal date in February was 10.36 d earlier from 1961 to 2013. The instability of extreme maxi- mum temperature in December and January improved. The instability of extreme minimum temperature in July instability also increased. The coefficient of variation of extreme minimum temperature in May was up to 287.3%. It is concluded that the instability of desert climate of Minqin County increased with global warming. The stability of climate is more worthy of attention.展开更多
The experiments of primary and secondary instabilities with controlled excitation are carried out on a swept flat plate to study the process leading to the final breakdown of laminar flow. Two types of high frequency ...The experiments of primary and secondary instabilities with controlled excitation are carried out on a swept flat plate to study the process leading to the final breakdown of laminar flow. Two types of high frequency secondary instabilities are identified. The most amplified mode is centered about the inflection point of the crosswise profile of the boundary layer and is interpreted as inflectional instability, the other occurs in the one third of the boundary layer from the wall. The high frequency disturbances are highly amplified but they also saturate similarly to the primary and nonlinearly generated disturbances. Their main effect in the final breakdown seems interact with the disturbances is developed and thus widens the frequency spectrum to turbulent state.展开更多
The robust stabilizating control problem for a class of uncertain nonlinear large-scale systems is discussed. Based on the theory of both input/output (I/O) linearization and decentralized variable structure control (...The robust stabilizating control problem for a class of uncertain nonlinear large-scale systems is discussed. Based on the theory of both input/output (I/O) linearization and decentralized variable structure control (VSC),two-level and decentralized variable structure control laws for this kind of systems are presented respectively,which achieve asymptotically stabilization despite the uncertainties and disturbances. At last,sirnulation of the disturbed two-pendulum system is given to illustrate the feasibility of proposed technique.展开更多
基金Projects(52378411,52208404)supported by the National Natural Science Foundation of China。
文摘The tunnel subjected to strike-slip fault dislocation exhibits severe and catastrophic damage.The existing analysis models frequently assume uniform fault displacement and fixed fault plane position.In contrast,post-earthquake observations indicate that the displacement near the fault zone is typically nonuniform,and the fault plane position is uncertain.In this study,we first established a series of improved governing equations to analyze the mechanical response of tunnels under strike-slip fault dislocation.The proposed methodology incorporated key factors such as nonuniform fault displacement and uncertain fault plane position into the governing equations,thereby significantly enhancing the applicability range and accuracy of the model.In contrast to previous analytical models,the maximum computational error has decreased from 57.1%to 1.1%.Subsequently,we conducted a rigorous validation of the proposed methodology by undertaking a comparative analysis with a 3D finite element numerical model,and the results from both approaches exhibited a high degree of qualitative and quantitative agreement with a maximum error of 9.9%.Finally,the proposed methodology was utilized to perform a parametric analysis to explore the effects of various parameters,such as fault displacement,fault zone width,fault zone strength,the ratio of maximum fault displacement of the hanging wall to the footwall,and fault plane position,on the response of tunnels subjected to strike-slip fault dislocation.The findings indicate a progressive increase in the peak internal forces of the tunnel with the rise in fault displacement and fault zone strength.Conversely,an augmentation in fault zone width is found to contribute to a decrease in the peak internal forces.For example,for a fault zone width of 10 m,the peak values of bending moment,shear force,and axial force are approximately 46.9%,102.4%,and 28.7% higher,respectively,compared to those observed for a fault zone width of 50 m.Furthermore,the position of the peak internal forces is influenced by variations in the ratio of maximum fault displacement of the hanging wall to footwall and the fault plane location,while the peak values of shear force and axial force always align with the fault plane.The maximum peak internal forces are observed when the footwall exclusively bears the entirety of the fault displacement,corresponding to a ratio of 0:1.The peak values of bending moment,shear force,and axial force for the ratio of 0:1 amount to approximately 123.8%,148.6%,and 111.1% of those for the ratio of 0.5:0.5,respectively.
文摘Aimed at the problem of stochastic routings for reprocessing operations and highly variable processing times,an open queuing network is utilized to model a typical reprocessing system.In the model,each server is subject to breakdown and has a finite buffer capacity,while repair times,breakdown times and service time follow an exponential distribution.Based on the decomposition principle and the expansion methodology,an approximation analytical algorithm is proposed to calculate the mean reprocessing time,the throughput of each server and other parameters of the processing system.Then an approach to determining the quality of disassembled parts is suggested,on the basis of which the effect of parts quality on the performance of the reprocessing system is investigated.Numerical examples show that there is a negative correlation between quality of parts and their mean reprocessing time.Furthermore,marginal reprocessing time of the parts decrease with the drop in their quality.
基金The US National Science Foundation (No. CMMI-0408390,CMMI-0644552)the American Chemical Society Petroleum Research Foundation(No. PRF-44468-G9 )+2 种基金Chang Jiang Scholars Program,the Fok Ying-Tong Education Foundation (No. 114024 )the Natural Science Foundation of Jiangsu Province(No. SBK200910046 )the Postdoctoral Science Foundation of Jiangsu Province (No.0901005C)
文摘Taking variability and uncertainty involved in performance prediction into account, in order to make the prediction reliable and meaningful, a distribution-based method is developed to predict future PSI. This method, which is based on the AASHTO pavement performance model, treats predictor variables as random variables with certain probability distributions and obtains the distribution of future PSI through the method of Monte-Carlo simulation. A computer program PERFORM using Monte Carlo simulation is developed to implement the numerical computation. Simulation results based on pavement and traffic parameters show that traffic, surface layer material property, and initial pavement performance are the most significant factors affecting pavement performance. Once the distribution of future PSI is determined, statistics such as the mean and the variance of future PSI are readily available.
基金Innovation Group Development Project of the Ministry of Education of China (No.IRT0435)Superexcellence Doctorial Dissertation Fund from Ministry of Education of China (No.200054).
文摘Pingxiang-dominant genic male sterile rice (PDGMSR) was the first dominant genic male sterile mutant identified in rice (Oryza sativa L.), and the corresponding dominant genic male sterile gene was designated as Ms-p. The fertility of PDGMSR can be restored by introduction of a dominant epistatic fertility restoring gene in some rice varieties. In the present study, E823, an indica inbred rice variety, restored the fertility of PDGMSR, and the genetic pattern was found to be consistent with a dominant epistatic model, therefore, the dominant epistatic fertility restorer gene was designated as Rfe. The F2 population from the cross of PDGMSR/E823 was developed to map gene Rfe. The F2 plants with the genotypes Ms-pMs-pRferfe or Ms-pms-pRferfe were used to construct a fertile pool, and the corresponding sterile plants with genotypes Ms-pMs-prferfe or Ms-pms-prferfe were used to con- struct a sterile pool. The fertility restoring gene Rfe was mapped to one side of the microsatellite markers RM311 and RM3152 on rice chromosome 10, with genetic distances of 7.9 cM and 3.6 cM, respectively. The microsatellite markers around the location of the Ms-p gene were used to finely map the Ms-p gene. The findings of this study indicated that the microsatellite markers RM171 and RM6745 flanked the Ms-p gene, and the distances were 0.3 cM and 3.0 cM, respectively. On the basis of the sequence of rice chromosome 10, the physical distance between the two markers is approximately 730 kb. These findings facilitates molecular marker-assisted selection (MAS) of genes Ms-p and Rfe in rice breeding programs, and cloning them in the future.
文摘To reduce the high computational cost of the uncertainty analysis, a procedure is proposed for the aerodynamic optimization under uncertainties, in which the surrogate model is used to simplify the computation of the uncertainty analysis. The surrogate model is constructed by using the Latin Hypercube design and the Kriging model. The random parameters are used to account for the small manufacturing errors and the variations of operating conditions. Based on the surrogate model, an uncertainty analysis approach, called the Monte Carlo simulation, is used to compute the mean value and the variance of the predicated performance. The robust optimization for aerodynamic design is formulated, and solved by the genetic algorithm. And then, an airfoil optimization problem is used to test the proposed procedure. Results show that the optimal solutions obtained from the uncertainty-based optimization formulation are less sensitive to uncertainties. And the design constraints are still satisfied under the uncertainties.
基金Science and Technology Support Planning of Jiangsu Province(No.BE2014133)the Open Foundation of Key Laboratory of Underw ater Acoustic Signal Processing(No.UASP1301)the Prospective Joint Research Project of Jiangsu province(No.BY2014127-01)
文摘To take into account the influence of uncetainties on the dynamic response of the vibro-acousitc structure, a hybrid modeling technique combining the finite element method(FE)and the statistic energy analysis(SEA) is proposed to analyze vibro-acoustics responses with uncertainties at middle frequencies. The mid-frequency dynamic response of the framework-plate structure with uncertainties is studied based on the hybrid FE-SEA method and the Monte Carlo(MC)simulation is performed so as to provide a benchmark comparison with the hybrid method. The energy response of the framework-plate structure matches well with the MC simulation results, which validates the effectiveness of the hybrid FE-SEA method considering both the complexity of the vibro-acoustic structure and the uncertainties in mid-frequency vibro-acousitc analysis. Based on the hybrid method, a vibroacoustic model of a construction machinery cab with random properties is established, and the excitations of the model are measured by experiments. The responses of the sound pressure level of the cab and the vibration power spectrum density of the front windscreen are calculated and compared with those of the experiment. At middle frequencies, the results have a good consistency with the tests and the prediction error is less than 3. 5dB.
基金supported by Natural Science Fund for colleges and universities in Jiangsu Province(No. 07KJD170012)Natural Science Fund of Huaihai Institute of Technology (No. Z2008009)
文摘Coastal sediment type map has been widely used in marine economic and engineering activities, but the traditional mapping methods had some limitations due to their intrinsic assumption or subjectivity. In this paper, a non-parametric indicator Kriging method has been proposed for generating coastal sediment map. The method can effectively avoid mapping subjectivity, has no special requirements for the sample data to meet second-order stationary or normal distribution, and can also provide useful information on the quantitative evaluation of mapping uncertainty. The application of the method in the southern sea area of Lianyungang showed that much more convincing mapping results could be obtained compared with the traditional methods such as IDW, Kriging and Voronoi diagram under the same condition, so the proposed method was applicable with great utilization value.
基金The Program for Special Talent in Six Fields of Jiangsu Province(No.2013ZBZZ-046)the Program of Lanzhou Technology Development(No.2014-1-175)
文摘Aimed at the remanufacturing system, the effect of the uncertainty of returns' quality on bottleneck shifting is investigated. A novel definition of bottleneck station is presented and the probability of a station becoming a bottleneck is also given. By calculating the effective output, the effective operation time (EOT) and the ratio of EOT of each station, the system's current bottleneck of effective output time is determined. By calculating the probability coefficient of variation and index of bottleneck shifting, the quantitative performance of bottleneck shifting is obtained. Discrete event simulation and the experiment design method are adopted to simulate the system, in which the proportion of quality grading, repair rates and process routes are considered. The case study shows that the uncertainty of returns' quality greatly increases the probability of bottleneck shifting, and with the increase of the discrete degree of the returns' repair rate, the bottleneck shifting phenomenon is more obvious. Furthermore, bottleneck shifting is closely related to the process route of the dominating returns' quality grade.
基金This project was supported by "the Hundred Leading Scientists Program of the Public Health Sector of Shanghai " (No. 98BR007), and "the National Science Foundation of China" (No. 30370645).
文摘Objective: To study the features of microsatellite alterations on chromosome 8 and their asso- ciation with clinicopathological characteristics of hepatocellular carcinoma (HCC). Methods: Ten highly- polymorphic microsatellite markers on chromosome 8 were selected to be detected for loss of heterozygosity (LOH), microsatellite instability (MSI) and allelic imbalance (AI) in 56 HCC using MegaBACE 500 auto- matic DNA analysis system. Results: LOH was found in 37 of 56 HCC (66.1%) on at least 1 locus, and the top three loci were D8S261(53.5%), D8S1721(52.5%) and D8S1771(52.5%). LOH frequency on D8S277 was signi?cantly higher in cases positive for serum HBsAg than in those negative for HBsAg (P <0.01). Similarly, LOH on D8S261, D8S298 and D8S1733 occurred more frequently in patients with negative HB- sAg than those with positive HBsAg (P <0.01). LOH on D8S298 and D8S1771 was more frequent in those tumors larger than 3 cm in size (P <0.05 or P <0.01). LOH frequencies of D8S1721 were signi?cantly higher in the patients with absent or not intact tumor capsule than in those with intact tumor capsule (P <0.05). LOH on D8S298 and D8S1771 was more frequently detected in tumors with intrahepatic metastasis than in those without intrahepatic metastasis (P <0.01). MSI was found in 12.5% (7/56) cases, and AI was found in 19.6% (11/56), no correlation was found between MSI and AI and clinicopathological character- istics of HCC. Conclusion: Frequent microsatellite alterations on chromosome 8 existed in HCC. LOH, which represents tumor suppressor gene pathway, plays a more important role in hepatocarcinogenesis; MSI representing mismatch repair gene pathway ranks next. LOH at a particula locus is associated with the clinicopathological parameters of human HCC.
基金supported by NSFC(No.41174118)one of the major state S&T special projects(No.2008ZX05020-004)+1 种基金a Postdoctoral Fellowship of China(No.2013M530106)China Scholarship Council(No.2010644006)
文摘In acoustic logging-while-drilling (ALWD) finite difference in time domain (FDTD) simulations, large drill collar occupies, most of the fluid-filled borehole and divides the borehole fluid into two thin fluid columns (radius -27 mm). Fine grids and large computational models are required to model the thin fluid region between the tool and the formation. As a result, small time step and more iterations are needed, which increases the cumulative numerical error. Furthermore, due to high impedance contrast between the drill collar and fluid in the borehole (the difference is 〉30 times), the stability and efficiency of the perfectly matched layer (PML) scheme is critical to simulate complicated wave modes accurately. In this paper, we compared four different PML implementations in a staggered grid finite difference in time domain (FDTD) in the ALWD simulation, including field-splitting PML (SPML), multiaxial PML(M- PML), non-splitting PML (NPML), and complex frequency-shifted PML (CFS-PML). The comparison indicated that NPML and CFS-PML can absorb the guided wave reflection from the computational boundaries more efficiently than SPML and M-PML. For large simulation time, SPML, M-PML, and NPML are numerically unstable. However, the stability of M-PML can be improved further to some extent. Based on the analysis, we proposed that the CFS-PML method is used in FDTD to eliminate the numerical instability and to improve the efficiency of absorption in the PML layers for LWD modeling. The optimal values of CFS-PML parameters in the LWD simulation were investigated based on thousands of 3D simulations. For typical LWD cases, the best maximum value of the quadratic damping profile was obtained using one do. The optimal parameter space for the maximum value of the linear frequency-shifted factor (a0) and the scaling factor (β0) depended on the thickness of the PML layer. For typical formations, if the PML thickness is 10 grid points, the global error can be reduced to 〈1% using the optimal PML parameters, and the error will decrease as the PML thickness increases.
文摘In cultivated rice ( Oryza sativa L.), F-1 pollen sterility is controlled by at least 6 loci of the F, pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-b(j)/S-b(j) and its near-isogenic line TIST2 carrying S-b(i)/S-b(i) were used to develop the mapping population. One hundred and fifty-eight microsatellite markers were selected to survey T65 and TISL2. RM13 on chromosome 5 was found to be polymorphic between them. Cosegregation indicated that RM13 was closely linked with locus S-b. Eleven RFLP markers were selected on the corresponding region from the genetic map of Rice Genome Research Program (RGP) of Japan to convert into sequence-tagged site (STS) markers. Amplicon length polymorphism (ALP) was carried out, but none of them was found to be polymorphic between T65 and TISL2. Then PCR-based RFLP (PBR) was done using six 4-nucleotide recognizing restriction endonucleases. Polymorphism was detected when PCR products of R830STS and R2213SSTS were digested with Taq I. Genetic analysis indicated that the distance between locus S-b and markers, R830STS, RM13 and R2213SSTS were 3.3 cM (centi-Morgan), 5.2 cM and 5.5 cM, respectively. These PCR-based markers could be directly used in marker-assisted selection. The technical system combining genetic mapping and PCR-based marker-assisted selection will facilitate the development of molecular breeding.
文摘Aim The solvability condition for robust stabilization problem associated with a plant family P(s,δ) having parameter uncertainty δ was considered. Methods Using Youla parameterization of the stabilizers this problem was transformed into a strong stabilization problem associated with a related plant family G (s, δ). Results A necessary solvability condition was established in terms of the parity interlacing property of each element in G(s,δ). Another apparently necessary solvability condition is that every element in P(s,δ) must be stabilizable. Conclusion The two conditions will be compared with each other and it will be shown that every element in G(s,δ) possesses parity interlacing property if P(s,δ) is stabilizable.
基金Supported by National High-tech R&D Program of China(2001AA241104, 2004AA241104)Key Breeding Project of Sichuan Province (200107001-1-6-1, 2006YZGG-5)Key Quality Project of Sichuan Province(2006YZGG-23)~~
文摘[ Objective] The study was to investigate the agronomic traits and breeding characteristics of genic male sterile material Mian 7AB-4- 2 in Brassica napus. [ Method] The differences in agronomic traits and polymorphisms in SSR markers, between the genic male sterile material Mian 7AB-4-2 in Brassica napus and its sisterly line Mian 7AB-4-1 were investigated by hybridization and molecular identification; and the percentage of sterile individuals of Mian 7AB-4-2 and of the hybrids with its sisterly line Mian 7AB-4-1 from test cross and back cross were also studled. [ Result] Mian 7AB-4-2 was not significantly different in agronomic traits from its sisterly line Mian 7AB-4-1 at 0.05 probability level. The per- centages of sterile individuals in the pollinated fertile Mian 7AB-4-2 plants were over 60%, and that in its sisterly line Mian 7AB-4-1 was about 25%. In test crosses with other nine sterile lines, Mian 7AB-4-1 kept the percentage of sterile individuals of sterile lines over 90%, and the percentage of sterile individuals from back cross over 80%. With regard to molecular markers, Mian 7AB-4-2 and its sisterly line Mian 7AB-4-1 were different in the band number from SSR primers a2 and E10. [ Conclusion] The results indicate that Mian 7AB-4-2 is helpful for rapeseed breeding, quickening the application of new materials in field breeding.
文摘We propose a new method for robust adaptive backstepping control of nonlinear systems with parametric uncertainties and disturbances in the strict feedback form. The method is called dynamic surface control. Traditional backstepping algorithms require repeated differentiations of the modelled nonlinearities. The addition of n first order low pass filters allows the algorithm to be implemented without differentiating any model nonlinearities, thus ending the complexity arising due to the 'explosion of terms' that makes other methods difficult to implement in practice. The combined robust adaptive backstepping/first order filter system is proved to be semiglobally asymptotically stable for sufficiently fast filters by a singular perturbation approach. The simulation results demonstrate the feasibility and effectiveness of the controller designed by the method.
基金Supported by the Fund of Ningxia Academy of Agriculture and Forestry Sciences(NKYJ-13-24)~~
文摘[Objective] This study was conducted to identify the thermo-sensitivity of the male sterile line (NYB) of Ningxia hot pepper. [Method] The pol en abortion rates of pepper male sterile line under artificial y control ed temperature condition and nature temperature condition were measured and compared. [Result] The pol en abortion was the sign of NYB male sterility. The pol en abortion rate changed little under different temperature conditions, while pol en amount greatly reduced at low temperature, indicating that NYB was genetical y stable in field. [Conclusion] The re-sults can provide some theoretical references for the application of NYB pepper male sterile line in breeding work.
基金Supported by the Major State Basic Research Development Program(973 Program)(2014CB460611)~~
文摘In order to study the stability of desert climate, the instability of climate in Minqin desert in response to global warming during 1961-2013 was analyzed by sliding standard deviation and a linear trend line. The results show that the instabili- ty of average temperature in January and April increased, and the isothermal date in February was 10.36 d earlier from 1961 to 2013. The instability of extreme maxi- mum temperature in December and January improved. The instability of extreme minimum temperature in July instability also increased. The coefficient of variation of extreme minimum temperature in May was up to 287.3%. It is concluded that the instability of desert climate of Minqin County increased with global warming. The stability of climate is more worthy of attention.
文摘The experiments of primary and secondary instabilities with controlled excitation are carried out on a swept flat plate to study the process leading to the final breakdown of laminar flow. Two types of high frequency secondary instabilities are identified. The most amplified mode is centered about the inflection point of the crosswise profile of the boundary layer and is interpreted as inflectional instability, the other occurs in the one third of the boundary layer from the wall. The high frequency disturbances are highly amplified but they also saturate similarly to the primary and nonlinearly generated disturbances. Their main effect in the final breakdown seems interact with the disturbances is developed and thus widens the frequency spectrum to turbulent state.
文摘The robust stabilizating control problem for a class of uncertain nonlinear large-scale systems is discussed. Based on the theory of both input/output (I/O) linearization and decentralized variable structure control (VSC),two-level and decentralized variable structure control laws for this kind of systems are presented respectively,which achieve asymptotically stabilization despite the uncertainties and disturbances. At last,sirnulation of the disturbed two-pendulum system is given to illustrate the feasibility of proposed technique.