[Objective] This study aimed to establish Balb/c mice model for food allergy caused by Chinese lobsters through using intraperitoneal injection for sensitization and explore methods for in vitro identification and eva...[Objective] This study aimed to establish Balb/c mice model for food allergy caused by Chinese lobsters through using intraperitoneal injection for sensitization and explore methods for in vitro identification and evaluation of food allergy caused by Chinese lobsters. [Method] The 40 male Bal/c mice were divided into ovalbumin(OVA) positive control group, Coca's solution negative control group, blank control group and model group. Balb/c mice model was established by intraperitoneally injection of immunized Balb/c mice with OVA or Chinese lobster crude protein with aluminum hydroxide adjuvant. IgE and histamine levels in serum after the second challenge were determined by ELISA method, and the specific IgE antibody titer was determined by passive cutaneous anaphylaxis test(PCA); additionally, spleen index and histological changes in the small intestine, as well as food allergy symptoms after challenge were also calculated or observed. [Results] After the last challenge, IgE content was(236.75 ±73.39) μg/L in the Chinese lobster crude protein group, revealing no difference with that in the OVA group, but significantly higher than that in either the Coca's solution group or the blank control group(P 0.01);histamine content in serum in the Chinese lobster crude protein group was(406.55±232.79), significantly higher than that in the blank control group(P0.01). In the passive cutaneous anaphylaxis test, IgE antibody titer reached 1/16 after the last challenge in the Chinese lobster crude protein group. Spleen index in both Chinese lobster crude protein group and OVA group was significantly greater than that in either the Coca's solution group or the blank control group(P0.01). What's more, infiltration of inflammatory cells like lymphocytes and eosnophils at the lamina propria of intestinal mucosa was also observed both Chinese lobster crude protein group and OVA group. [Conclusion] This study established Balb/c mice model for food allergy caused by Chinese lobsters; serum IgE and ELISA assay and specific IgE antibody titer in PCA test can be used for the in vitro identification and evaluation of food allergies caused by Chinese lobsters.展开更多
The Longchi area with the city of Dujiangyan, in the Sichuan province of China, is composed of Permian stone and diorites and Triassic sandstones and mudstones intercalated with slates. An abundance of loose co-seismi...The Longchi area with the city of Dujiangyan, in the Sichuan province of China, is composed of Permian stone and diorites and Triassic sandstones and mudstones intercalated with slates. An abundance of loose co-seismic materials were present on the slopes after the May 12, 2008 Wenchuan earthquake, which in later years served as source material for rainfall-induced debris flows or shallow landslides. A total of 48 debris flows, all triggered by heavy rainfall on 13th August 20l0, are described in this paper. Field investigation, supported by remote sensing image interpretation, was conducted to interpret the co-seismic landslides in the debris flow gullies. Specific characteristics of the study area such as slope, aspect, elevation, channel gradient, lithology, and gully density were selected for the evaluation of debris flow susceptibility. A score was given to all the debris flow gullies based on the probability of debris flow occurrence for the selected factors. In order to get the contribution of the different factors, principal component analyses were applied. A comprehensive score was obtained for the 48 debris flow gullies which enabled us to make a susceptibility map for debris flows with three classes. Twenty-two gullies have a high susceptibility, twenty gullies show a moderate susceptibility and six gullies have a low susceptibility for debris flows.展开更多
Lower Silurian Longmaxi Shale(SLS) in southeastern Sichuan Basin, China, was analyzed for major and selected trace elements, and their provenance, intensity of palaeoweathering of the source rocks were analyzed based ...Lower Silurian Longmaxi Shale(SLS) in southeastern Sichuan Basin, China, was analyzed for major and selected trace elements, and their provenance, intensity of palaeoweathering of the source rocks were analyzed based on these elements. The results show that SiO_2, Al_2O_3 and Fe_2O_3, are dominant major elements with average contents of 60.59%, 15.91% and 5.87% in Upper Silurian Longmaxi Shale(USLS), and 65.14%, 13.24% and 4.68% in Lower Silurian Longmaxi Shale(LSLS). The TiO_2-Zr plot, Hf(ppm) versus La/Th discriminant diagram, and abundance of Cr and Ni suggest a dominantly felsic source for the Longmaxi sediments. Average chemical index of alteration(CIA), plagioclase index of alteration(PIA) values(64.05% and 72.86%, respectively) imply low-degree chemical weathering of the source material in early Longmaxi time, and average CIA, PIA values(68.44% and 80.35%, respectively) imply moderate chemical weathering of the source material in late Longmaxi time.展开更多
Phytoplankton blooms occurring in the Jiangdong Reservoir of Jiulong River,Fujian Province,South China,are a potential source of contamination of the drinking water of Xiamen(Amoy)City.To understand the main factors g...Phytoplankton blooms occurring in the Jiangdong Reservoir of Jiulong River,Fujian Province,South China,are a potential source of contamination of the drinking water of Xiamen(Amoy)City.To understand the main factors governing phytoplankton composition and succession,we sampled phytoplankton and measured environmental parameters in the reservoir,weekly or biweekly from Jan.2010 to Feb.2012.We identifi ed 123 species of phytoplankton from 7 phyla and 74 genera.The major phyla were Chlorophyta,Bacillariophyta,Cryptophyta,Cyanophyta,and Dinophyta.The main trend in the succession of phytoplankton was from prevalence of Cryptophyta-Bacillariophyta communities to those of Chlorophyta-Cyanophyta.High cell concentrations of Cryptophyta,predominantly Komma caudate,Cryptomonas marssonii,and Cryptomonas erosa,were present in winter,associated with low river discharge and cold water.Bacillariophyta,primarily Cyclotella meneghiniana,Aulacoseira granulata,and Aulacoseira granulata var.angustissima,dominated in early spring,coinciding with high turbulence and low irradiance.During early summer and autumn,Chlorophyta,comprising Scenedesmus quadricauda,Dictyosphaerium ehrenbergianum,and Pandorina sp.were prevalent during conditions of warmer water temperatures and low turbulence.Cyanophyta,with dominance of Pseudanabaena mucicola,Merismopedia tenuissima and Raphidiopsis sp.increased throughout the summer,coinciding with higher water temperatures and lower nutrient concentrations.Dinophyta content was occasionally high during winter and summer.Peridiniopsis penardii(Dinophyta)bloomed during winter 2009,with a persistently high biomass recorded into early spring.Canonical correspondence analysis indicated that phytoplankton communities were infl uenced by river discharge,irradiance,water temperature,and nutrient concentrations.展开更多
Water samples were collected at 20 sites on 4 occasions in 2009 (twice in May, and once in both August and November) along the Jiulong River, South China to examine how nutrient inputs from the Jiulong River could a...Water samples were collected at 20 sites on 4 occasions in 2009 (twice in May, and once in both August and November) along the Jiulong River, South China to examine how nutrient inputs from the Jiulong River could affect the nutrient status of the Xiamen Water. Samples were analyzed for nitrite (NO2-N), nitrate (NO3-N), ammonium (NH4-N), phosphate (PO4-P), silicate (SiO3-Si), salinity, and temperature, to determine the nutrient and trophic status of the river. The results indicate that nutrients are derived mainly from river runoff. NO3-N was the main form of DIN in most parts of the river. In addition, NO3-N, DIN, and SiO3-Si behave conservatively. There is a surplus of DIN and SiO3-Si in the river, and PO4-P is a limitation on phytoplankton growth. The concentration of DIN is typically above 0.60 mg/dm3, and higher than 1.00 mg/dm3 in most parts of the river. The concentration of PO4-P is typically above 0.02 mg/dm3, while the concentration of SiO3-Si is higher than 1.00 rag/din3. Between 2003 and 2008, samples were collected 3 times per year (May, August and November) at 27 sites in the Xiamen Water and analyzed for NO2-N, NO3-N, NH4-N, PO4-P, salinity, and temperature. We discovered that the Jiulong River was the key source of DIN into the Xiamen Water, but not PO4-P, indicating the reason of the N/P molar ratio imbalance in the Xiamen Water. In the future, the effects of high DIN concentrations on the phytoplankton communities and marine ecosystems of the Xiamen Water shall be studied.展开更多
AIM: To investigate the relationship between mutations of rearranged during transfection (RET) proto-oncogene and Chinese patients with Hirschsprung's disease (HD), and to elucidate the genetic mechanism of famili...AIM: To investigate the relationship between mutations of rearranged during transfection (RET) proto-oncogene and Chinese patients with Hirschsprung's disease (HD), and to elucidate the genetic mechanism of familial HD patient at the molecular level.METHODS: Genomic DNA was extracted from venous blood of probands and their relatives in two genealogies.Polymerase chain reaction (PCR) products, which were amplified using specific primers (RET, exons 11, 13, 15and 17), were electrophoresed to analyze the single-strand conformational polymorphism (SSCP) patterns. The positive amplified products were sequenced. Forty-eight sporadic HD patients and 30 normal children were screened for mutations of RET proto-oncogene simultaneously.RESULTS: Three cases with HD in one family were found to have a G heterozygous insertion at nucleotide 18 974 in exon 13 of RET cDNA (18 974insG), which resulted in a frameshift mutation. In another family, a heterozygosity for T to G transition at nucleotide 18 888 in the same exon which resulted in a synonymous mutation of Leu at codon 745 was detected in the proband and his father. Eight RET mutations were confirmed in 48 sporadic HD patients.CONCLUSION: Mutations of RET proto-oncogene may play an important role in the pathogenesis of Chinese patients with HD. Detection of mutated RET proto-oncogene carriers may be used for genetic counseling of potential risk for HD in the affected families.展开更多
In 1998, the Chinese Government implemented the NFPP (Natural Forest Protection Program), which included logging restrictions, protected areas, replanting, and a range of other policies aimed at safeguarding the sta...In 1998, the Chinese Government implemented the NFPP (Natural Forest Protection Program), which included logging restrictions, protected areas, replanting, and a range of other policies aimed at safeguarding the state of the country's forests and reducing the risk of erosion and flooding. A second phase of this program is currently being discussed. In this paper, contingent valuation is used to estimate the WTP (willingness to pay) for maintaining the program among the inhabitants in Heilongjiang Province in northern China. The results show that, even with fairly conservative assumptions, the aggregated WTP for maintaining the program for another five years is some 3.24 billion yuan per year. This can be compared with the current cost of the Program in the province, which is some 1.57 billion yuan per year.展开更多
Four main dinosaur-bearing sites have been investigated in latest Cretaceous deposits from the Amur/Heilongjiang Region : Jiayin and Wulaga in China ( Yuliangze Formation), Blagoveschensk and Kundur in Russia (Udu...Four main dinosaur-bearing sites have been investigated in latest Cretaceous deposits from the Amur/Heilongjiang Region : Jiayin and Wulaga in China ( Yuliangze Formation), Blagoveschensk and Kundur in Russia (Udurchukan Formation). More than 90% of the bones discovered in these localities belong to hollow-crested lambeosaurine hadrosaurids: Charonosaurus fiayinensis at Jiayin, Amurosaurus riabinini at Blagoveschensk, Olorotitan arharensis at Kundur, and Sahaliyania elunchunorum at Wulaga. Flat-headed hadrosaurine hadrosaurids are much less numerous, but appear well diversified as well: Kerberosaurus manakini at Blagoveschensk, Wulagasaurus dongi at Wulaga, and a new genus at Kundur. Theropods are represented by shed teeth and isolated bones; isolated scutes and teeth discovered at Kundur are tentatively attributed to nodosaurids. Palynological studies suggest that these sites are probably synchronous with the Lancian' vertebrate localities of western North America, which represent the youngest dinosaur faunas in this area. However, the latest Cretaceous dinosaur assemblages are completely different in the Amur/Heilongjiang region (lambeosaurines abundant, ceratopsids absent) and in western North America (ceratopsids abundant, lainbeosaurines extremely rare or absent). This probably reflects some kind of geographical barrier between both areas by Maastrichtian time rather than strong differences in palaeoecological conditions.展开更多
The palynological assemblage from Wulaga dinosaur site in Jiayin from southeastern Zeya-Bureya Basin was studied. The high content of pteridophytes spores is characteristic for this assemblage. The further characters ...The palynological assemblage from Wulaga dinosaur site in Jiayin from southeastern Zeya-Bureya Basin was studied. The high content of pteridophytes spores is characteristic for this assemblage. The further characters are in decrease of abundance-tricolpate pollen, Ginkgocycadophytus, pollen of Taxodiaceae, Cupressaceae and Taxaceae (TCT), bisaccate pollen of Pinaceae, and triporate pollen. The bone-bearing burials of Wulaga dinosaur site formed under conditions of vast swampy river valley covered by ferns and taxodialeans. Tricolpate pollen, produced by plants, close to those of Platanaceae, Fagaceae, Betulaceae, and Juglandaceae, counts in favor of valley river open canopy forests.展开更多
The Cheng-Lan railway links Chengdu, a central city in Southwestern China, and Lanzhou, a central city in Northwestern China. The railway passes through the Longmenshan fault zone (Wenchuan earthquake happened there o...The Cheng-Lan railway links Chengdu, a central city in Southwestern China, and Lanzhou, a central city in Northwestern China. The railway passes through the Longmenshan fault zone (Wenchuan earthquake happened there on May 12, 2008), Minjiang fault zone, and Dongkunlun fault zone, which are all active. It runs over the Yangtze River and the Yellow River, and crosses high mountains and deep valleys. There exists, along the railway's alignment, different kinds of strata of hard granite and soft, weak metamorphic rocks such as carbonaceous slate, schist, and phyllite. It is, therefore, a key issue for such an infrastructure construction to assess the engineering geological conditions and risks, so as to mitigate or avoid possible georisks and to offer optional designs. Geological survey and georisk assessment along the railway corridor are carried out. Special attention is given to active faults, earthquakes and seismic zones. Based on these, discussions about geological aspects for route selection of the railway are conducted and countermeasures for georisk control are proposed accordingly. Main conclusions are achieved as follows: (1) Geohazards such as landslides, rockfalls and debries flows dominate both the route selection of the railway and the engineering structures (e.g., tunnels or bridges) adopted; (2) Tunnel has been proved to be an excellent structure for linear engineering in geologically active area; and (3) In the case where avoiding is impractical, necessary protection measures should be taken to engineering slopes in high earthquake intensity areas, especially the area with earthquake of Ms. 8 or greater.展开更多
The types, composition and physico-chemical conditions of primary fluid inclusions were researched. The results show that the primary fluid inclusions contain vapor and liquid phase type (Type I), daughter mineral-b...The types, composition and physico-chemical conditions of primary fluid inclusions were researched. The results show that the primary fluid inclusions contain vapor and liquid phase type (Type I), daughter mineral-bearing type (Type II) and pure liquid phase type (Type III). The compositions of vapor are mainly H20 and CO2 with a tiny amounts of CH4 and H2; the liquid phase mainly contains Mg2+, Ca2+, Na+, K+, CI- and SO]-, and w(Na+)/w(K+)〉l; the homogenization temperatures of the primary fluid inclusions can be divided into 190-250 ℃, 250-340 ℃ and 360-420 ℃, corresponding to the salinities of 4%-9%, 9%-14%, and 14%-20.43% (NaC1 equivalent mass fraction), respectively. The mineralization process can be divided into three episodes: the silicatization stage, the quartz-sulfide stage, and the carbonatization stage, and all of them are associated with the ore-forming hydrothermal fluid activity. The origin of the hydrothermal fluid is from magrnatic water mainly, and later it mixes with the groundwater and meteoric water, which lead to the decrease of temperature and salinity. The decrease of salinity, temperature and pressure are the main causes of the metallogenic elements unloading and enriching in the favorable position.展开更多
The aquatic ecosystem maybe significantly affected by polycyclic aromatic hydrocarbons(PAHs) released from fresh water sediments. In order to protect biodiversity, the spatial distribution and sources of PAHs in the s...The aquatic ecosystem maybe significantly affected by polycyclic aromatic hydrocarbons(PAHs) released from fresh water sediments. In order to protect biodiversity, the spatial distribution and sources of PAHs in the sediment of Wolong Lake in Northeast China were studied. A total of 17 surface sediment samples were collected and 12 PAHs were analyzed. The results were as follows. The concentration of total PAHs(TPAHs) ranged between 1412.9 μg/kg and 3948.3 μg/kg(dry weight). Indeno [1, 2, 3-c, d] pyrene was the dominant contaminant which accounted for 87%–98% of TPAHs. Diagnostic ratios of PAHs and principal component analysis showed that biomass combustion and vehicle emissions were likely to be the dominant sources of PAHs in the sediment. PAHs can be considered safe in the context of environmental and human health protection, based on the overall toxicity. Individual PAHs were positively correlated with total organic carbons. These results will be helpful to control PAHs and protect the aquatic ecosystem in the lake.展开更多
The ability to manage and restore plant communities in the face of human-induced landscape change may rely on our ability to predict how species respond to environmental variables.Understanding this response requires ...The ability to manage and restore plant communities in the face of human-induced landscape change may rely on our ability to predict how species respond to environmental variables.Understanding this response requires examining factors or their interactions that have influence on plant and resource availability.Our objective was to analyze the relationships between changes in plant abundance and the interaction among environmental habitat factors including soil, geological(rock type), and other environmental variables in the Longhushan karst mountains ecosystem.Species density and dominance were examined using ANOVA, ANCOVA,and Generalized Linear Models to establish the single or combined effects of these groups of factors.The results showed that trends in abundance were mainly affected by rock type(related to the percentage content of dolomite and calcite), soil characteristics in association with topography.Both plant indices were higher in dolomite dominated areas and varied positively with moisture, and elevation, but negatively with organic matter, while density also increased with slope degree.The results demonstrate that significant variations in species abundance was produced with the combination of variables from soil, geological, andenvironmental factors, suggesting their interaction influence on plants.We postulate that spatial variations in plant abundance in karst ecosystem depends on the carbonate rock type in addition to water and nutrient availability which are mainly controlled by topography and other factors such as soil texture and temperature.The study suggests that in karst areas carbonate rock type, in addition to local environmental variables, should be taken into account when analyzing the factors that have impact on plant communities.展开更多
As one of the top ten Chinese teas, Longjing Green Tea is famous for its green tea leaves, sweet taste, pleasant aroma and beautiful shape. Besides of using as drinks, Longjing tea leaves can be applied as spice in Ch...As one of the top ten Chinese teas, Longjing Green Tea is famous for its green tea leaves, sweet taste, pleasant aroma and beautiful shape. Besides of using as drinks, Longjing tea leaves can be applied as spice in Chinese cuisine for its particular taste and function. Under the background of pursuing healthy cuisine, the development of organic Longjing tea and advocate of Chinese tea culture have great economic and culture significance.展开更多
基金Supported by the Science and Technology Project of Department of Education of Jiangxi Province(GJJ08399)~~
文摘[Objective] This study aimed to establish Balb/c mice model for food allergy caused by Chinese lobsters through using intraperitoneal injection for sensitization and explore methods for in vitro identification and evaluation of food allergy caused by Chinese lobsters. [Method] The 40 male Bal/c mice were divided into ovalbumin(OVA) positive control group, Coca's solution negative control group, blank control group and model group. Balb/c mice model was established by intraperitoneally injection of immunized Balb/c mice with OVA or Chinese lobster crude protein with aluminum hydroxide adjuvant. IgE and histamine levels in serum after the second challenge were determined by ELISA method, and the specific IgE antibody titer was determined by passive cutaneous anaphylaxis test(PCA); additionally, spleen index and histological changes in the small intestine, as well as food allergy symptoms after challenge were also calculated or observed. [Results] After the last challenge, IgE content was(236.75 ±73.39) μg/L in the Chinese lobster crude protein group, revealing no difference with that in the OVA group, but significantly higher than that in either the Coca's solution group or the blank control group(P 0.01);histamine content in serum in the Chinese lobster crude protein group was(406.55±232.79), significantly higher than that in the blank control group(P0.01). In the passive cutaneous anaphylaxis test, IgE antibody titer reached 1/16 after the last challenge in the Chinese lobster crude protein group. Spleen index in both Chinese lobster crude protein group and OVA group was significantly greater than that in either the Coca's solution group or the blank control group(P0.01). What's more, infiltration of inflammatory cells like lymphocytes and eosnophils at the lamina propria of intestinal mucosa was also observed both Chinese lobster crude protein group and OVA group. [Conclusion] This study established Balb/c mice model for food allergy caused by Chinese lobsters; serum IgE and ELISA assay and specific IgE antibody titer in PCA test can be used for the in vitro identification and evaluation of food allergies caused by Chinese lobsters.
基金supported by the Key Projects in the National Science & Technology Pillar Program (Grant No. 2011BAK12B01)Basic Scientific Project of Ministry of Sciences and Technology of China (Grant No. 2011FY110100-3)
文摘The Longchi area with the city of Dujiangyan, in the Sichuan province of China, is composed of Permian stone and diorites and Triassic sandstones and mudstones intercalated with slates. An abundance of loose co-seismic materials were present on the slopes after the May 12, 2008 Wenchuan earthquake, which in later years served as source material for rainfall-induced debris flows or shallow landslides. A total of 48 debris flows, all triggered by heavy rainfall on 13th August 20l0, are described in this paper. Field investigation, supported by remote sensing image interpretation, was conducted to interpret the co-seismic landslides in the debris flow gullies. Specific characteristics of the study area such as slope, aspect, elevation, channel gradient, lithology, and gully density were selected for the evaluation of debris flow susceptibility. A score was given to all the debris flow gullies based on the probability of debris flow occurrence for the selected factors. In order to get the contribution of the different factors, principal component analyses were applied. A comprehensive score was obtained for the 48 debris flow gullies which enabled us to make a susceptibility map for debris flows with three classes. Twenty-two gullies have a high susceptibility, twenty gullies show a moderate susceptibility and six gullies have a low susceptibility for debris flows.
基金Project(41302076)supported by the National Natural Science Foundation of ChinaProject(BJ14266)supported by MOST Special Fund from the State Key Laboratory of Continental Dynamics,Northwest University,ChinaProject(2014JQ5191)supported by Natural Science Basic Research Plan of Shaanxi Province,China
文摘Lower Silurian Longmaxi Shale(SLS) in southeastern Sichuan Basin, China, was analyzed for major and selected trace elements, and their provenance, intensity of palaeoweathering of the source rocks were analyzed based on these elements. The results show that SiO_2, Al_2O_3 and Fe_2O_3, are dominant major elements with average contents of 60.59%, 15.91% and 5.87% in Upper Silurian Longmaxi Shale(USLS), and 65.14%, 13.24% and 4.68% in Lower Silurian Longmaxi Shale(LSLS). The TiO_2-Zr plot, Hf(ppm) versus La/Th discriminant diagram, and abundance of Cr and Ni suggest a dominantly felsic source for the Longmaxi sediments. Average chemical index of alteration(CIA), plagioclase index of alteration(PIA) values(64.05% and 72.86%, respectively) imply low-degree chemical weathering of the source material in early Longmaxi time, and average CIA, PIA values(68.44% and 80.35%, respectively) imply moderate chemical weathering of the source material in late Longmaxi time.
基金Supported by the Bureau of Science and Technology of Xiamen Municipal Government,China(No.3502Z20091005)the National Natural Science Foundation of China(No.40925018)the State Oceanic Administration(SOA)Program(Nos.201105021,201005015)
文摘Phytoplankton blooms occurring in the Jiangdong Reservoir of Jiulong River,Fujian Province,South China,are a potential source of contamination of the drinking water of Xiamen(Amoy)City.To understand the main factors governing phytoplankton composition and succession,we sampled phytoplankton and measured environmental parameters in the reservoir,weekly or biweekly from Jan.2010 to Feb.2012.We identifi ed 123 species of phytoplankton from 7 phyla and 74 genera.The major phyla were Chlorophyta,Bacillariophyta,Cryptophyta,Cyanophyta,and Dinophyta.The main trend in the succession of phytoplankton was from prevalence of Cryptophyta-Bacillariophyta communities to those of Chlorophyta-Cyanophyta.High cell concentrations of Cryptophyta,predominantly Komma caudate,Cryptomonas marssonii,and Cryptomonas erosa,were present in winter,associated with low river discharge and cold water.Bacillariophyta,primarily Cyclotella meneghiniana,Aulacoseira granulata,and Aulacoseira granulata var.angustissima,dominated in early spring,coinciding with high turbulence and low irradiance.During early summer and autumn,Chlorophyta,comprising Scenedesmus quadricauda,Dictyosphaerium ehrenbergianum,and Pandorina sp.were prevalent during conditions of warmer water temperatures and low turbulence.Cyanophyta,with dominance of Pseudanabaena mucicola,Merismopedia tenuissima and Raphidiopsis sp.increased throughout the summer,coinciding with higher water temperatures and lower nutrient concentrations.Dinophyta content was occasionally high during winter and summer.Peridiniopsis penardii(Dinophyta)bloomed during winter 2009,with a persistently high biomass recorded into early spring.Canonical correspondence analysis indicated that phytoplankton communities were infl uenced by river discharge,irradiance,water temperature,and nutrient concentrations.
基金Supported by the Scientific Research Foundation of Third Institute of Oceanography,SOA(Nos.TIO2007009,TIO2009007)the River Basin-Estuary Ecological Security Assessment and Management Strategy(No.200805064)+3 种基金the Natural Science Foundation of Fujian Province(Nos.2006J0362,2010J01260,2012Y0048)the National Department Public Benefit Research Foundation:Protection Technique of Beach and Demonstration Project in China(No.200905008)a Sub-task of the National Commonweal Marine Research Project:Typical Semi-enclosed Bay Eutrophication Immediate Report and Ecological Effect Assessment(No.201105014-6)the Special Social Commonweal Foundation for Research Institutes:Primary Red Tide Warning Index System of Physical and Chemical Research for Enclosed Bay(No.2004DIB3J084)
文摘Water samples were collected at 20 sites on 4 occasions in 2009 (twice in May, and once in both August and November) along the Jiulong River, South China to examine how nutrient inputs from the Jiulong River could affect the nutrient status of the Xiamen Water. Samples were analyzed for nitrite (NO2-N), nitrate (NO3-N), ammonium (NH4-N), phosphate (PO4-P), silicate (SiO3-Si), salinity, and temperature, to determine the nutrient and trophic status of the river. The results indicate that nutrients are derived mainly from river runoff. NO3-N was the main form of DIN in most parts of the river. In addition, NO3-N, DIN, and SiO3-Si behave conservatively. There is a surplus of DIN and SiO3-Si in the river, and PO4-P is a limitation on phytoplankton growth. The concentration of DIN is typically above 0.60 mg/dm3, and higher than 1.00 mg/dm3 in most parts of the river. The concentration of PO4-P is typically above 0.02 mg/dm3, while the concentration of SiO3-Si is higher than 1.00 rag/din3. Between 2003 and 2008, samples were collected 3 times per year (May, August and November) at 27 sites in the Xiamen Water and analyzed for NO2-N, NO3-N, NH4-N, PO4-P, salinity, and temperature. We discovered that the Jiulong River was the key source of DIN into the Xiamen Water, but not PO4-P, indicating the reason of the N/P molar ratio imbalance in the Xiamen Water. In the future, the effects of high DIN concentrations on the phytoplankton communities and marine ecosystems of the Xiamen Water shall be studied.
基金Supported by the Fund for Excellent Young Talented Persons by Public Health Ministry of China, and Analysis and Testing Foundation of Zhejiang Province, No. 99075
文摘AIM: To investigate the relationship between mutations of rearranged during transfection (RET) proto-oncogene and Chinese patients with Hirschsprung's disease (HD), and to elucidate the genetic mechanism of familial HD patient at the molecular level.METHODS: Genomic DNA was extracted from venous blood of probands and their relatives in two genealogies.Polymerase chain reaction (PCR) products, which were amplified using specific primers (RET, exons 11, 13, 15and 17), were electrophoresed to analyze the single-strand conformational polymorphism (SSCP) patterns. The positive amplified products were sequenced. Forty-eight sporadic HD patients and 30 normal children were screened for mutations of RET proto-oncogene simultaneously.RESULTS: Three cases with HD in one family were found to have a G heterozygous insertion at nucleotide 18 974 in exon 13 of RET cDNA (18 974insG), which resulted in a frameshift mutation. In another family, a heterozygosity for T to G transition at nucleotide 18 888 in the same exon which resulted in a synonymous mutation of Leu at codon 745 was detected in the proband and his father. Eight RET mutations were confirmed in 48 sporadic HD patients.CONCLUSION: Mutations of RET proto-oncogene may play an important role in the pathogenesis of Chinese patients with HD. Detection of mutated RET proto-oncogene carriers may be used for genetic counseling of potential risk for HD in the affected families.
文摘In 1998, the Chinese Government implemented the NFPP (Natural Forest Protection Program), which included logging restrictions, protected areas, replanting, and a range of other policies aimed at safeguarding the state of the country's forests and reducing the risk of erosion and flooding. A second phase of this program is currently being discussed. In this paper, contingent valuation is used to estimate the WTP (willingness to pay) for maintaining the program among the inhabitants in Heilongjiang Province in northern China. The results show that, even with fairly conservative assumptions, the aggregated WTP for maintaining the program for another five years is some 3.24 billion yuan per year. This can be compared with the current cost of the Program in the province, which is some 1.57 billion yuan per year.
基金the Belgian State,Federal Scientific Policy,S&T bilateral co-operation project BL/36/C22-R12National Geographic Society project 6970-01+4 种基金Jurassic FoundationFWOFNRSNSFC Project 30220130698supported by the Fonds pour la Formationàla Recherche dans l'Industrie et dans l'Agriculture
文摘Four main dinosaur-bearing sites have been investigated in latest Cretaceous deposits from the Amur/Heilongjiang Region : Jiayin and Wulaga in China ( Yuliangze Formation), Blagoveschensk and Kundur in Russia (Udurchukan Formation). More than 90% of the bones discovered in these localities belong to hollow-crested lambeosaurine hadrosaurids: Charonosaurus fiayinensis at Jiayin, Amurosaurus riabinini at Blagoveschensk, Olorotitan arharensis at Kundur, and Sahaliyania elunchunorum at Wulaga. Flat-headed hadrosaurine hadrosaurids are much less numerous, but appear well diversified as well: Kerberosaurus manakini at Blagoveschensk, Wulagasaurus dongi at Wulaga, and a new genus at Kundur. Theropods are represented by shed teeth and isolated bones; isolated scutes and teeth discovered at Kundur are tentatively attributed to nodosaurids. Palynological studies suggest that these sites are probably synchronous with the Lancian' vertebrate localities of western North America, which represent the youngest dinosaur faunas in this area. However, the latest Cretaceous dinosaur assemblages are completely different in the Amur/Heilongjiang region (lambeosaurines abundant, ceratopsids absent) and in western North America (ceratopsids abundant, lainbeosaurines extremely rare or absent). This probably reflects some kind of geographical barrier between both areas by Maastrichtian time rather than strong differences in palaeoecological conditions.
基金Supported by project"111" of China (No.B06008)supported by Far East Branch of Russian Academy of Sciences(grants 06-Ⅲ-A-06-141, 09-1-P15-02) Russian Foundation for basic researches (grant 07-05-00168)
文摘The palynological assemblage from Wulaga dinosaur site in Jiayin from southeastern Zeya-Bureya Basin was studied. The high content of pteridophytes spores is characteristic for this assemblage. The further characters are in decrease of abundance-tricolpate pollen, Ginkgocycadophytus, pollen of Taxodiaceae, Cupressaceae and Taxaceae (TCT), bisaccate pollen of Pinaceae, and triporate pollen. The bone-bearing burials of Wulaga dinosaur site formed under conditions of vast swampy river valley covered by ferns and taxodialeans. Tricolpate pollen, produced by plants, close to those of Platanaceae, Fagaceae, Betulaceae, and Juglandaceae, counts in favor of valley river open canopy forests.
基金supported by a grant from the Major State Basic Research Development Program of China(973Program)(Grant No.2013CB733202)the team research fund of the State Key Laboratory of Geohazards Prevention and Geoenvironment Protection(Grant No.SKLGP)
文摘The Cheng-Lan railway links Chengdu, a central city in Southwestern China, and Lanzhou, a central city in Northwestern China. The railway passes through the Longmenshan fault zone (Wenchuan earthquake happened there on May 12, 2008), Minjiang fault zone, and Dongkunlun fault zone, which are all active. It runs over the Yangtze River and the Yellow River, and crosses high mountains and deep valleys. There exists, along the railway's alignment, different kinds of strata of hard granite and soft, weak metamorphic rocks such as carbonaceous slate, schist, and phyllite. It is, therefore, a key issue for such an infrastructure construction to assess the engineering geological conditions and risks, so as to mitigate or avoid possible georisks and to offer optional designs. Geological survey and georisk assessment along the railway corridor are carried out. Special attention is given to active faults, earthquakes and seismic zones. Based on these, discussions about geological aspects for route selection of the railway are conducted and countermeasures for georisk control are proposed accordingly. Main conclusions are achieved as follows: (1) Geohazards such as landslides, rockfalls and debries flows dominate both the route selection of the railway and the engineering structures (e.g., tunnels or bridges) adopted; (2) Tunnel has been proved to be an excellent structure for linear engineering in geologically active area; and (3) In the case where avoiding is impractical, necessary protection measures should be taken to engineering slopes in high earthquake intensity areas, especially the area with earthquake of Ms. 8 or greater.
基金Project(200911007-04) supported by the Special Funds for Scientific Research of Land and Natural Resources, ChinaProject (2007CB411405) supported by the National Basic Research Program of ChinaProject(20109901) supported by the National Crisis Office of China
文摘The types, composition and physico-chemical conditions of primary fluid inclusions were researched. The results show that the primary fluid inclusions contain vapor and liquid phase type (Type I), daughter mineral-bearing type (Type II) and pure liquid phase type (Type III). The compositions of vapor are mainly H20 and CO2 with a tiny amounts of CH4 and H2; the liquid phase mainly contains Mg2+, Ca2+, Na+, K+, CI- and SO]-, and w(Na+)/w(K+)〉l; the homogenization temperatures of the primary fluid inclusions can be divided into 190-250 ℃, 250-340 ℃ and 360-420 ℃, corresponding to the salinities of 4%-9%, 9%-14%, and 14%-20.43% (NaC1 equivalent mass fraction), respectively. The mineralization process can be divided into three episodes: the silicatization stage, the quartz-sulfide stage, and the carbonatization stage, and all of them are associated with the ore-forming hydrothermal fluid activity. The origin of the hydrothermal fluid is from magrnatic water mainly, and later it mixes with the groundwater and meteoric water, which lead to the decrease of temperature and salinity. The decrease of salinity, temperature and pressure are the main causes of the metallogenic elements unloading and enriching in the favorable position.
基金Under the auspices of National Natural Science Foundation of China(No.41101295)Shenyang Science and Technology Projects(No.JJ2011-13)
文摘The aquatic ecosystem maybe significantly affected by polycyclic aromatic hydrocarbons(PAHs) released from fresh water sediments. In order to protect biodiversity, the spatial distribution and sources of PAHs in the sediment of Wolong Lake in Northeast China were studied. A total of 17 surface sediment samples were collected and 12 PAHs were analyzed. The results were as follows. The concentration of total PAHs(TPAHs) ranged between 1412.9 μg/kg and 3948.3 μg/kg(dry weight). Indeno [1, 2, 3-c, d] pyrene was the dominant contaminant which accounted for 87%–98% of TPAHs. Diagnostic ratios of PAHs and principal component analysis showed that biomass combustion and vehicle emissions were likely to be the dominant sources of PAHs in the sediment. PAHs can be considered safe in the context of environmental and human health protection, based on the overall toxicity. Individual PAHs were positively correlated with total organic carbons. These results will be helpful to control PAHs and protect the aquatic ecosystem in the lake.
基金founded by the National Natural Scientific Foundation of China(Grant No.40972218)the Fundamental Research Founds for National University,China University of Geosciences(Wuhan)(Grant Nos.G1323521125,G1323521225,G1323521325)
文摘The ability to manage and restore plant communities in the face of human-induced landscape change may rely on our ability to predict how species respond to environmental variables.Understanding this response requires examining factors or their interactions that have influence on plant and resource availability.Our objective was to analyze the relationships between changes in plant abundance and the interaction among environmental habitat factors including soil, geological(rock type), and other environmental variables in the Longhushan karst mountains ecosystem.Species density and dominance were examined using ANOVA, ANCOVA,and Generalized Linear Models to establish the single or combined effects of these groups of factors.The results showed that trends in abundance were mainly affected by rock type(related to the percentage content of dolomite and calcite), soil characteristics in association with topography.Both plant indices were higher in dolomite dominated areas and varied positively with moisture, and elevation, but negatively with organic matter, while density also increased with slope degree.The results demonstrate that significant variations in species abundance was produced with the combination of variables from soil, geological, andenvironmental factors, suggesting their interaction influence on plants.We postulate that spatial variations in plant abundance in karst ecosystem depends on the carbonate rock type in addition to water and nutrient availability which are mainly controlled by topography and other factors such as soil texture and temperature.The study suggests that in karst areas carbonate rock type, in addition to local environmental variables, should be taken into account when analyzing the factors that have impact on plant communities.
文摘As one of the top ten Chinese teas, Longjing Green Tea is famous for its green tea leaves, sweet taste, pleasant aroma and beautiful shape. Besides of using as drinks, Longjing tea leaves can be applied as spice in Chinese cuisine for its particular taste and function. Under the background of pursuing healthy cuisine, the development of organic Longjing tea and advocate of Chinese tea culture have great economic and culture significance.