Dichlorodiphenyltrichloroethane (DDT),tributyltin (TBT),and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are persistent in the environment and cause continuous toxic effects in humans and aquatic life.Tetrahymena thermo...Dichlorodiphenyltrichloroethane (DDT),tributyltin (TBT),and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are persistent in the environment and cause continuous toxic effects in humans and aquatic life.Tetrahymena thermophila has the potential for use as a model for research regarding toxicants.In this study,this organism was used to analyze a genome-wide microarray generated from cells exposed to DDT,TBT and TCDD.To accomplish this,genes differentially expressed when treated with each toxicant were identified,after which their functions were categorized using GO enrichment analysis.The results suggested that the responses of T.thermophila were similar to those of multicellular organisms.Additionally,the context likelihood of relatedness method (CLR) was applied to construct a TCDD-relevant network.The T-shaped network obtained could be functionally divided into two subnetworks.The general functions of both subnetworks were related to the epigenetic mechanism of TCDD.Based on analysis of the networks,a model of the TCDD effect on T.thermophila was inferred.Thus,Tetrahymena has the potential to be a good unicellular eukaryotic model for toxic mechanism research at the genome level.展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos. 30870356 and 31071993) to Miao Weithe Knowledge Innovation Program of Chinese Academy of Sciences (Grant No. KSCX2-YW-G-072) to Miao Wei
文摘Dichlorodiphenyltrichloroethane (DDT),tributyltin (TBT),and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are persistent in the environment and cause continuous toxic effects in humans and aquatic life.Tetrahymena thermophila has the potential for use as a model for research regarding toxicants.In this study,this organism was used to analyze a genome-wide microarray generated from cells exposed to DDT,TBT and TCDD.To accomplish this,genes differentially expressed when treated with each toxicant were identified,after which their functions were categorized using GO enrichment analysis.The results suggested that the responses of T.thermophila were similar to those of multicellular organisms.Additionally,the context likelihood of relatedness method (CLR) was applied to construct a TCDD-relevant network.The T-shaped network obtained could be functionally divided into two subnetworks.The general functions of both subnetworks were related to the epigenetic mechanism of TCDD.Based on analysis of the networks,a model of the TCDD effect on T.thermophila was inferred.Thus,Tetrahymena has the potential to be a good unicellular eukaryotic model for toxic mechanism research at the genome level.
