The surface and adhesion forces between chitosan- coated mica surfaces in an acetic acid buffer solution were measured using a surface force apparatus （SFA）. The force- distance profiles were obtained under differen...The surface and adhesion forces between chitosan- coated mica surfaces in an acetic acid buffer solution were measured using a surface force apparatus （SFA）. The force- distance profiles were obtained under different pressure conditions. It was found that the chitosan was adsorbed on the mica surface and formed a stable nanofilm under acid conditions. The adsorbed chitosan nanofilms induced a short- range monotonically steric force when two such surfaces came close in the acid buffer. The adhesion forces between the two chitosan-coated mica surfaces varied with the loads. Strong adhesion between the two chitosan-coated mica surfaces was observed at high pressure. Such pressure-dependent adhesion properties are most likely related to the molecular configurations and hydrogen bonds reordering under high confinement.展开更多
Objective To investigate the effect of Coriolus versicolor polysaccharide-B （CVPs-B） on the biological characteristics of human esophageal carcinoma cell line Ecal09 in vitro. Methods The cells of experimental group...Objective To investigate the effect of Coriolus versicolor polysaccharide-B （CVPs-B） on the biological characteristics of human esophageal carcinoma cell line Ecal09 in vitro. Methods The cells of experimental group （EG） were cultured in DMEM with 10% FCS and 150μg/mL CVPs-B, the cells of control group （CG） were cultured in DMEM with 10% FCS without CVPs-B. MTT reduction assay was performed to detect the effect of CVPs-B on the proliferation of Ecal09 cells after the compound was administrated in varying concentrations. The living conditions of the Ecal09 cells were determined using trypan blue exclusion. Then, cell growth curves were drawn. Flow cytometry was performed to detect the effect of CVPs-B on the apoptosis and cell cycle of Ecal09. Results In comparison with the CG, a marked decrease in the proliferation of Eca09 cells was observed in the EG, after incubation with CVPs-B. The survival rate of Eca09 cells decreased as the time of CVPs-B incubation prolonged. Comparing the cell cycles and apoptotic rates between the two groups, the proportions of cells in the G0/G1, S, and G2/M phases in the EG were found to be （68.4±3.7）%, （13.9±2.1）%, and （17.7±1.4）%, respectively, after 24 h incubation with CVPs-B. The cells had an apoptotic rate of （9.7±0.7）%. On the other hand, the proportions of the G0/G1, S, and G2/M cells of the CG were found to be （53.9±3.6）%, （26.6±2.8）%, and （19.5±2.3）%, respectively, with an apoptotic rate of （5.7±1.4）%. In comparison with the CG cells, significant cell growth in the G0/G1 phase was observed in the EG （P〈0.05）. Furthermore, a significant decrease in the number of cells in the S phase was observed （P〈0.05） in the EG. Conclusions CVPs-B can inhibit proliferation and enhance apoptosis of Ecal09 cells and may be useful in the treatment of esophageal carcinoma.展开更多
基金The National Basic Research Program of China(973Program)(No.2011CB707605)the National Natural Science Foundation of China(No.50925519)
文摘The surface and adhesion forces between chitosan- coated mica surfaces in an acetic acid buffer solution were measured using a surface force apparatus （SFA）. The force- distance profiles were obtained under different pressure conditions. It was found that the chitosan was adsorbed on the mica surface and formed a stable nanofilm under acid conditions. The adsorbed chitosan nanofilms induced a short- range monotonically steric force when two such surfaces came close in the acid buffer. The adhesion forces between the two chitosan-coated mica surfaces varied with the loads. Strong adhesion between the two chitosan-coated mica surfaces was observed at high pressure. Such pressure-dependent adhesion properties are most likely related to the molecular configurations and hydrogen bonds reordering under high confinement.
基金supported by the Guangdong Provincial Sci-Tech Planning(No.2010B030700051)
文摘Objective To investigate the effect of Coriolus versicolor polysaccharide-B （CVPs-B） on the biological characteristics of human esophageal carcinoma cell line Ecal09 in vitro. Methods The cells of experimental group （EG） were cultured in DMEM with 10% FCS and 150μg/mL CVPs-B, the cells of control group （CG） were cultured in DMEM with 10% FCS without CVPs-B. MTT reduction assay was performed to detect the effect of CVPs-B on the proliferation of Ecal09 cells after the compound was administrated in varying concentrations. The living conditions of the Ecal09 cells were determined using trypan blue exclusion. Then, cell growth curves were drawn. Flow cytometry was performed to detect the effect of CVPs-B on the apoptosis and cell cycle of Ecal09. Results In comparison with the CG, a marked decrease in the proliferation of Eca09 cells was observed in the EG, after incubation with CVPs-B. The survival rate of Eca09 cells decreased as the time of CVPs-B incubation prolonged. Comparing the cell cycles and apoptotic rates between the two groups, the proportions of cells in the G0/G1, S, and G2/M phases in the EG were found to be （68.4±3.7）%, （13.9±2.1）%, and （17.7±1.4）%, respectively, after 24 h incubation with CVPs-B. The cells had an apoptotic rate of （9.7±0.7）%. On the other hand, the proportions of the G0/G1, S, and G2/M cells of the CG were found to be （53.9±3.6）%, （26.6±2.8）%, and （19.5±2.3）%, respectively, with an apoptotic rate of （5.7±1.4）%. In comparison with the CG cells, significant cell growth in the G0/G1 phase was observed in the EG （P〈0.05）. Furthermore, a significant decrease in the number of cells in the S phase was observed （P〈0.05） in the EG. Conclusions CVPs-B can inhibit proliferation and enhance apoptosis of Ecal09 cells and may be useful in the treatment of esophageal carcinoma.
