电针“人中”穴的抗过敏性休克作用及与脑内神经降压肽含量的关系

目的 观察电针“人中”穴的抗过敏性休克作用及与脑内神经降压肽含量的关系，探讨电针“人中”穴的抗过敏性休克作用机制。方法 选用76只成年健康豚鼠，制成过敏性休克模型，以平均动脉压（MAP）为指标，观察同强度（电压3V）不同频率（4～16、30～40、80～100Hz）电针“人中”穴对过敏性休克低血压反应的影响，并用放射免疫法测定各脑区内神经降压肽（neurotensin，NT）的含量。结果 ①不同频率分别电针“人中”穴的升压效应均显著高于各自的非穴位电针对照组（P〈0.05，P〈0.01）；不同频率分别电针“人中”穴的三组之间以及非穴位电针三组之间MAP的变化差异无统计学意义。②正常组与过敏性休克组下丘脑、延髓、腺垂体、神经垂体中NT含量的变化差异均无统计学意义，而过敏性休克加不同频率电针“人中”穴各组的下丘脑、延髓、腺垂体、神经垂体中NT的含量均显著高于单纯休克组（P〈0．05，P〈0.01）。过敏性休克加不同频率电针“人中”穴三组间各脑区内NT含量的变化差异均无统计学意义。结论 ①电针“人中”穴对过敏性休克时的低血压反应确有迅速而良好的改善作用。②NT可能参与电针“人中”穴的抗过敏性休克作用。

针刺“人中”“内关”对脑出血大鼠血肿周围脑组织NGB、Cyt-C表达的影响

目的观察大鼠脑出血(ICH)急性期血肿周围脑组织脑红蛋白(NGB)、细胞色素C(Cyt-C)的表达随时间动态变化及针刺干预对其影响,探讨针刺"人中""内关"对大鼠ICH急性期的神经保护及细胞凋亡的影响。方法健康雄性SD大鼠96只随机分为对照组、模型组、穴位组、非穴组,每组24只。采用自体非肝素抗凝动脉血二次注入大鼠尾状核制作动物模型,符合纳入标准的大鼠再按出血后6、24、48、72 h分为4个时间点亚组,每组6只大鼠。穴位组采取针刺"人中""内关"干预;非穴组针刺非穴点干预;对照组及模型组捆绑束缚,干预后对应时间点取材。采用NSS评分法对术后大鼠神经功能缺损程度进行评分;运用免疫组化法定性测定血肿周围脑组织NGB、Cyt-C蛋白表达情况。结果与模型组相比,穴位组大鼠神经功能缺损体征明显降低,在72 h差异有统计学意义(P<0.05)。与模型组相比,穴位组各时间点NGB表达均增高(P<0.05)、Cyt-C表达均下降(P<0.05);与穴位组相比,非穴组各时间点NGB表达低于穴位组(P<0.05)、Cyt-C表达高于穴位组(P<0.05)。结论针刺"人中""内关"能改善ICH大鼠神经功能缺损体征,还可能通过提高出血后血肿周围脑组织NGB表达、降低Cyt-C表达,从而保护脑组织,抑制神经元凋亡,拮抗出血后继发的神经元损伤。

Clinical Study on Puncturing Renying （ST 9） to Treat Poststroke Dysphagia

Objective： To observe the clinical efficacy of puncturing Renying （ST 9） in the treatment of poststroke dysphagia. Methods： Sixty cases of poststroke dysphagia were randomized into two groups, a control group in which 30 cases were given rehabilitation training, and a treatment group in which 30 cases were treated by puncturing Renying （ST 9） and rehabilitation training, with a course of four weeks. Results： The total effective rate for dysphagia was higher in the treatment group than in the control group （P〈0.05）. Conclusion： Puncturing Renying （ST 9） is quite effective for poststroke dysphagia.

Effect of Sanhuangwuji powder,anti-rheumatic drugs,and ginger-partitioned acupoint stimulation on the treatment of rheumatoid arthritis with peptic ulcer:a randomized controlled study

OBjECTIVE:To observe the efficacy and safety of oral Sanhuangwuji powder,anti-rheumatic drugs(ARDs),and ginger-partitioned acupoint stimulation at Zusanli(ST 36) on the treatment of rheumatoid arthritis(RA) complicated by peptic ulcer.METHODS:This prospective randomized controlled study included 180 eligible inpatients and outpatients randomly assigned to an ARD treatment(n = 60),ginger-partitioned stimulation(n =60),or combination treatment(n = 60).Patients assigned to the ARD group were given oral celecoxib,methotrexate,and esomeprazole.Patients assigned to theginger-partitioned stimulation group were given ginger-partitioned acupoint stimulation at Zusanli(ST 36) in addition to the ARDs.Patients in the combination treatment group were given oral Sanhuangwuji powder,ginger-partitioned acupoint stimulation at Zusanli(ST 36),and ARDs.All patients were followed up for 2 months to evaluate clinical effects and safety.The study was registered in the World Health Organization database at the General Hospital of Chengdu Military Area Command Chinese People's Liberation Army(ChiCTR-TCC12002824).RESULTS:The combination treatment group had significantly greater improvements in RA symptoms,laboratory outcomes,and gastrointestinal symptom scores,compared with the other groups(P < 0.05).The peptic ulcer healing rate in the combination treatment group was significantly greater than that in the ARD treatment group(x^2 = 16.875,P < 0.05) and the ginger-partitioned stimulation group(x^2 = 6.171,P<0.05).CONCLUSION:Combination treatment with ginger-partitioned acupoint stimulation at Zusanli(ST36),oral Sanhuangwuji powder,and ARDs had a better clinical effect for RA with complicated peptic ulcer,compared with ARD treatmentalone or in combination with ginger-partitioned acupoint stimulation.