Aim To investigate the synergistic effect of the combination of pinellia total alkaloid （PTA） and uncaria total alkaloid （UTA）, and explore the mechanism of anticonvulsant action. Methods Anticonvulsant and toxic ...Aim To investigate the synergistic effect of the combination of pinellia total alkaloid （PTA） and uncaria total alkaloid （UTA）, and explore the mechanism of anticonvulsant action. Methods Anticonvulsant and toxic effect profiles of combinations of PTA with UTA, alone and at three fixed ratios of 1：4, 1 ：1, 4：1, were evaluated in maximal electroshock （MES）-induced seizures and acute toxicity test in mice. Respective ED50 and LD50 were calculated with Bliss＇s method. Their synergistic effect were evaluated by isobolographic analysis and allowed the determination of benefit indices （BI） for respective combinations. The model of convulsive rats kindled by penicillin topically injected into cortex was used to investigated the content of Glu, Asp, Gly and GABA in hippocampus using high performance liquid chromatography （HPLC）. Results Combinations of PTA and UTA at the ratio of 4：1 were synergistic in MES test and antagonistic in acute toxicity test, showing the best profile for combinations of PTA with UTA. In contrast, the ratios of 1 ：4 and 1 ： 1, despite synergistic in MES test, were additive in acute toxicity test. The 4：1 combination and two drugs alone significantly decreased Glu level and increased GABA level in the hippocampus, but the GABA level in the 4：1 combination group was higher than that in the two drugs alone groups. They did not have significant influence on the levels of ASp and Gly. Conclusion Combinations of PTA and UTA at 4：1 ratio demonstrated synergistic effect in anticonvulsant action and antagonistic effect in toxicity. The anticonvulsant mechanism might be related to decreasing the excitability of Glutamatergic neurons and increasing the inhibition of GABAergic neurons.展开更多
[Objective] The aim was to study the effect of different soak treatments on breaking seed dormancy in soil seed bank from different degraded grasslands. [Method] Different concentrations of H2SO4,GA3 and KNO3 were use...[Objective] The aim was to study the effect of different soak treatments on breaking seed dormancy in soil seed bank from different degraded grasslands. [Method] Different concentrations of H2SO4,GA3 and KNO3 were used for soaking the seeds in the soil seed bank from different degraded grasslands,and the germination number of seeds was detected. [Result] When the seeds from soil seed bank were soaked with 60%,70%,80% and 90% H2SO4,the germination number of seeds was 0,indicating that the germination of seeds was inhibited; when the seeds were soaked with GA3,the germination number of seeds increased with the concentration of GA3 increasing. When the concentration of GA3 increased to 0.10%,the germination of seeds was inhibited; when the seeds were soaked with 0.2% KNO3,the germination number of seeds was greater than the blank control. [Conclusion] The number of remaining seeds was more in the soil seed banks collected from moderately degraded grassland and heavily degraded grassland; while the number of remaining seeds was small in the soil seed banks collected from lightly degraded grassland and extremely degraded grassland.展开更多
Peltophorum dubium seeds provided by Anhembi, SP were scarified in 98% H2SO4 for 15 min to overcome mechani-cal dormancy. Seeds were primed in solutions of 0.2% Captan at 10 C and 27C, PEG 6000 -1.0 MPa at 10C and 27...Peltophorum dubium seeds provided by Anhembi, SP were scarified in 98% H2SO4 for 15 min to overcome mechani-cal dormancy. Seeds were primed in solutions of 0.2% Captan at 10 C and 27C, PEG 6000 -1.0 MPa at 10C and 27C, 0.5 mol KNO3, 0.75 Mol KNO3, 1.0 Mol KNO3. Eight treatments including the primed seeds and nonprimed seeds, five replicates with 100 seeds for each treatment, were set to 15-cm-Petri dish with double filter paper moistened with testing solution PEG in refrigerator at 27C. For the experiments of all the groups, osmotic potential were 0.0, -0.2, -0.4, -0.6, -0.8, -1.0, -1.2, and -1.4 MPa. P. dubium seeds were also set to water stress experiment in rolled paper with PEG solutions from 0.0 to -1.0 M Pa. Germination percentage decreased with the increase of PEG concentration. Control group had a better germination percentage than other groups. Germination hardly occurred in PEG -1.4 MPa.展开更多
[ Objective] The cold shock method was used to induce diploid gynogenesis of Silurus astus. [ Mthod ] The Silurus astus sperms were irradiated by ultraviolet, then conducted for fertilization. The different cold shock...[ Objective] The cold shock method was used to induce diploid gynogenesis of Silurus astus. [ Mthod ] The Silurus astus sperms were irradiated by ultraviolet, then conducted for fertilization. The different cold shock starting time and duration were set to observe diploid gynogenesis of Silurus astus. [ Result] Under such inducing condition that ultraviolet irradiated 15 min,cold shock started 5 rain after fertilization, and cold shock duartion was 40 rain,the survival rate of diploid Silurus astus reached the highest（8.5% ）. [ Conclusion] The experiment laid foundation for culturing new excellent Silurus astus variety and accumulated original files tor further study of gynogenesis development mechanism.展开更多
基金Natural Science Foundation of Shanxi Province(No 20041109).
文摘Aim To investigate the synergistic effect of the combination of pinellia total alkaloid （PTA） and uncaria total alkaloid （UTA）, and explore the mechanism of anticonvulsant action. Methods Anticonvulsant and toxic effect profiles of combinations of PTA with UTA, alone and at three fixed ratios of 1：4, 1 ：1, 4：1, were evaluated in maximal electroshock （MES）-induced seizures and acute toxicity test in mice. Respective ED50 and LD50 were calculated with Bliss＇s method. Their synergistic effect were evaluated by isobolographic analysis and allowed the determination of benefit indices （BI） for respective combinations. The model of convulsive rats kindled by penicillin topically injected into cortex was used to investigated the content of Glu, Asp, Gly and GABA in hippocampus using high performance liquid chromatography （HPLC）. Results Combinations of PTA and UTA at the ratio of 4：1 were synergistic in MES test and antagonistic in acute toxicity test, showing the best profile for combinations of PTA with UTA. In contrast, the ratios of 1 ：4 and 1 ： 1, despite synergistic in MES test, were additive in acute toxicity test. The 4：1 combination and two drugs alone significantly decreased Glu level and increased GABA level in the hippocampus, but the GABA level in the 4：1 combination group was higher than that in the two drugs alone groups. They did not have significant influence on the levels of ASp and Gly. Conclusion Combinations of PTA and UTA at 4：1 ratio demonstrated synergistic effect in anticonvulsant action and antagonistic effect in toxicity. The anticonvulsant mechanism might be related to decreasing the excitability of Glutamatergic neurons and increasing the inhibition of GABAergic neurons.
基金Supported by National Natural Science Foundation of China (30860061)National Science and Technology Support Program(2007BAC06B01)~~
文摘[Objective] The aim was to study the effect of different soak treatments on breaking seed dormancy in soil seed bank from different degraded grasslands. [Method] Different concentrations of H2SO4,GA3 and KNO3 were used for soaking the seeds in the soil seed bank from different degraded grasslands,and the germination number of seeds was detected. [Result] When the seeds from soil seed bank were soaked with 60%,70%,80% and 90% H2SO4,the germination number of seeds was 0,indicating that the germination of seeds was inhibited; when the seeds were soaked with GA3,the germination number of seeds increased with the concentration of GA3 increasing. When the concentration of GA3 increased to 0.10%,the germination of seeds was inhibited; when the seeds were soaked with 0.2% KNO3,the germination number of seeds was greater than the blank control. [Conclusion] The number of remaining seeds was more in the soil seed banks collected from moderately degraded grassland and heavily degraded grassland; while the number of remaining seeds was small in the soil seed banks collected from lightly degraded grassland and extremely degraded grassland.
基金This work is Supported by CAPES Brazil. Open research laboratory of forest plant ecology NEFU. The State's tenth five-year "211 Project"-supported key academic discipline program of ECNU。
文摘Peltophorum dubium seeds provided by Anhembi, SP were scarified in 98% H2SO4 for 15 min to overcome mechani-cal dormancy. Seeds were primed in solutions of 0.2% Captan at 10 C and 27C, PEG 6000 -1.0 MPa at 10C and 27C, 0.5 mol KNO3, 0.75 Mol KNO3, 1.0 Mol KNO3. Eight treatments including the primed seeds and nonprimed seeds, five replicates with 100 seeds for each treatment, were set to 15-cm-Petri dish with double filter paper moistened with testing solution PEG in refrigerator at 27C. For the experiments of all the groups, osmotic potential were 0.0, -0.2, -0.4, -0.6, -0.8, -1.0, -1.2, and -1.4 MPa. P. dubium seeds were also set to water stress experiment in rolled paper with PEG solutions from 0.0 to -1.0 M Pa. Germination percentage decreased with the increase of PEG concentration. Control group had a better germination percentage than other groups. Germination hardly occurred in PEG -1.4 MPa.
基金the Key Technology Research (0324030026)Development Program of Henan Province and Key Programs of Zoology of Henan Province~~
文摘[ Objective] The cold shock method was used to induce diploid gynogenesis of Silurus astus. [ Mthod ] The Silurus astus sperms were irradiated by ultraviolet, then conducted for fertilization. The different cold shock starting time and duration were set to observe diploid gynogenesis of Silurus astus. [ Result] Under such inducing condition that ultraviolet irradiated 15 min,cold shock started 5 rain after fertilization, and cold shock duartion was 40 rain,the survival rate of diploid Silurus astus reached the highest（8.5% ）. [ Conclusion] The experiment laid foundation for culturing new excellent Silurus astus variety and accumulated original files tor further study of gynogenesis development mechanism.
