企业创新秘诀：“优术、取势、明道”

在实践过程中，三者往往互为根本，道术合一。

High-yielding Seed Production Techniques and Characteristics of Hybrid Rice Yanliangyou 888

According to seed production of Yanliangyou 888, a two-line hybrid rice variety, the research reviewed seed production characters, as follows： to improve outcrossing rate and spike size provided sufficient spikes of female/male parents. The seed production techniques include scientific arrangement of seeding interval, cultivating strong multi-tiller seedlings, scientific arrangement of row ratio of inter- cropping with adequate basic seedlings, scientific water and fertilizer management, improving outcrossing rate by synthesis measures, disease/insect control, and rein- forcing production management.

Seed Production Techniques of Two-line Indica Hybrid Rice Yanliangyou 2208

Yanliangyou 2208, bred by the Jiangsu Institute of Agricultural Sciences in Coastal Areas, is a two-line indica hybrid rice combination characterized with strong heterosis, moderate maturity, wide adaptability, good stability and disease resistance. It was identified as a new variety by the National Crop Variety Approval Committee in 2013. According to the characteristics of parents of Yanliangyou 2208 and com- bined with many years of seed production experience, high-yielding seed production techniques of Yanliangyou 2208 are summed up in this paper.

Study on Establishment of cpSSR Marker Technology and Optimization of Its Reaction System in Jatropha curcas Linn

[ Objective] The aim of this study was to establish the optimum cpSSR-PCR system for Jatropha curcas Linn. [ Method] cpSSR-PCR amplification system for Jatropha curcas Linn influenced by five factors including Taq DNA polymerase, Mg^2＋ , DNA template, dNTP and primer were optimized from several levels. [ Result] The optimum concentration of 20 μl reaction system was 10 × Buffer, 2.00 mmol/L Mg^2＋ , 2 U/μl Taq DNA polymerase, 0.2 mmol/L dNTP, 0.2 μmol/L primer and 35 ng/μl DNA template. [ Conclusion] The optimum annealing temperature for cpSSR-PCR reaction system is 52 ℃, and the cpSSR reaction system is steady and reproducible.

SCDI Flash Memory Device Ⅱ:Experiments and Characteristics

Step channel direct injection(SCDI) flash memory device is successfully achieved by 1 2μm CMOS technology,moreover good performance is obtained.At the bias condition of V g=6V, V d=5V,the programming speed of SCDI device is 42μs.Under the condition of V g=-8V, V s=8V,the erasing speed is 24ms.Compared with the same size of conventional flash memory device whose corresponding parameters are 500μs and 24ms,respectively,the performance of SCDI device is remarkably improved.During manufacturing of SCDI device,the key technologies are to make the shallow step with appropriate depth and angle,along with eliminating the etch damage during the process of Si 3N 4 spacer.

Variation Dynamics of Total Nitrogen and Nicotine of Burley Tobacco at Various Growing Stages under Different Nitrogen Nutrition Levels

[Objective] This study aimed to investigate the variation dynamics of total nitrogen and nicotine of burtey tobacco at various growing stages under different ni- trogen nutrition levels to provide reference for optimizing fertilization technologies for burley tobacco. [Method] Fresh burley tobacco leaves were collected at various growing stages and dried to measure the content of total nitrogen, protein and nico- tine by using INTEGRAL automated chemical analyzer and analyze the correlation with nitrogen application level. [Result] Regardless of the nitrogen application level, the content of total nitrogen and protein showed a downward trend since root ex- tending stage and reached the minimum at mature stage; the content of nicotine showed an upward trend since early vigorous growing stage and increased to the maximum at mature stage; the content of total nitrogen, protein and nicotine all in- creased after air curing; the content of total nitrogen, protein and nicotine showed positive correlation with nitrogen application level, while total nitrogen/nicotine was negatively correlated. The results indicate that nitrogen level is closely related to the total nitrogen and nicotine of burley tobacco at growing period and post-air curing, rational application of nitrogen is an important measure to regulate the content of total nitrogen and nicotine of tobacco. [Conclusion] This study provides scientific ba- sis for rational fertilization of burley tobacco.

Performance and Cultivation Techniques of a Promising Super Hybrid Rice Combination Chaoyou 1000 in High-latitude Area

The performance and cultivation techniques of Chaoyou 1000 in Junan County, Shandong Province were summed up.

Application of EBSD technique to ultrafine grained and nanostructured materials processed by severe plastic deformation:Sample preparation, parameters optimization and analysis

With the help of FESEM, high resolution electron backscatter diffraction can investigate the grains/subgrains as small as a few tens of nanometers with a good angular resolution （~0.5°）. Fast development of EBSD speed （up to 1100 patterns per second） contributes that the number of published articles related to EBSD has been increasing sharply year by year. This paper reviews the sample preparation, parameters optimization and analysis of EBSD technique, emphasizing on the investigation of ultrafine grained and nanostructured materials processed by severe plastic deformation （SPD）. Detailed and practical parameters of the electropolishing, silica polishing and ion milling have been summarized. It is shown that ion milling is a real universal and promising polishing method for EBSD preparation of almost all materials. There exists a maximum value of indexed points as a function of step size. The optimum step size depends on the magnification and the board resolution/electronic step size. Grains/subgrains and texture, and grain boundary structure are readily obtained by EBSD. Strain and stored energy may be analyzed by EBSD.

Intrahepatic biliary cystadenoma:Is there really an almost exclusively female predominance?

Biliary cystic tumors,such as cystadenomas and cystadenocarcinomas,are rare cystic tumors of the liver,accounting for less than 5% of all intrahepatic cysts of biliary origin.Biliary cystadenomas have been known to occur predominantly in women (> 85%),and 38%-44% of biliary cystadenocarcinomas have occurred in males.We wrote this letter to comment on a brief article (World J Gasteroenterol 2011 January 21;17(3):361-365) regarding a case of intrahepatic biliary cystic neoplasm treated with surgery.The adenoma-carcinoma sequence is the possible mechanism of carcinogenesis.If the carcinogenesis of biliary cystadenocarcinoma occurs in the adenoma-carcinoma sequence,we believe that the male-to-female ratio of cystadenoma should be higher than the incidence rate that has been reported to date.

Simulation and Optimization in the Process of Toluene Liquid-phase Catalytic Oxidation

Liquid-phase oxidation of toluene with air has become the main technology for producing benzoic acid in a reactor at present. Based on the kinetic model of the toluene oxidation process obtained from laboratory and mass balance of key component, a novel model is established to simulate the industrial toluene oxidation process, in which the effects of benzaldehyde and benzyl alcohol are considered and the kinetic parameters are revised by industrial data. The simulation results show that the error of benzoic acid yield is within 3.5%. Based on the simulation model, to maximize the benzoic acid yield, an optimization model is proposed to optimize the operating parameters, including toluene feed-in mass flux and temperature. The optimization result indicates that on the allowable operating conditions the maximum benzoic acid yield obtained with the reaction temperature at 167.2 C an the mass flux at 104.1 t·h^- 1 is greater than the current one, which can be used to guide industrial reactor s operation.

Bearing Capacity and Technical Advantages of Composite Bucket Foundation of Offshore Wind Turbines

Based on mechanical characteristics such as large vertical load, large horizontal load, large bending moment and complex geological conditions, a large scale composite bucket foundation （CBF） is put forward. Both the theoretical analysis and numerical simulation are employed to study the bearing capacity of CBF and the relationship between loads and ground deformation. Furthermore, monopile, high-rise pile cap, tripod and CBF designs are compared to analyze the bearing capacity and ground deformation, with a 3-MW wind generator as an example. The resuits indicate that CBF can effectively bear horizontal load and large bending moment resulting from upper structures and environmental load.

Technical research on optimization design of contacts of electrical connector

Pin and socket contacts are the key parts of electrical connector as aerospace electronic components. The contacts are also the direct carriers for signal transmitting of electronic equipments, passing the signal from the input end to the output end of electrical connector. The reliability of pin and socket contacts directly influences signal transmission. The goal of this study is to enhance the contact reliability of aerospace electrical connector. Computer simulation analysis was made on contacts performance data by utilizing the simulation system developed by PCL Language of MSC software. Furthermore, the results were experi- mentally validated so as to realize the objective of optimizing contacts design.

Quality of Service Routing Strategy Using Supervised Genetic Algorithm

A supervised genetic algorithm (SGA) is proposed to solve the quality of service (QoS) routing problems in computer networks. The supervised rules of intelligent concept are introduced into genetic algorithms (GAs) to solve the constraint optimization problem. One of the main characteristics of SGA is its searching space can be limited in feasible regions rather than infeasible regions. The superiority of SGA to other GAs lies in that some supervised search rules in which the information comes from the problems are incorporated into SGA. The simulation results show that SGA improves the ability of searching an optimum solution and accelerates the convergent process up to 20 times.

Optimized Technology for Residuum Processing in the ARGG Unit

The influence of feedstock property on operation in the FCC unit was studied to identify the cause leading to deteriorated products distribution related with increasingly heavier feedstock for the ARGG unit. In order to maximize the economic benefits of the ARGG unit a string of measures, including the modification of catalyst formulation, retention of high catalyst activity, application of mixed termina-tion agents to control the reaction temperature and once-through operation, and optimization of catalyst regeneration technique, were adopted to adapt the ARGG unit to processing of the heavy feedstock with its carbon residue equating to 7 % on an average. The heavy oil processing technology has brought about apparent economic benefits.

Parentage Analysis of Dongfang No.2, a Hybrid of a Female Gametophyte Clone of Laminaria japonica (Laminariales, Phaeophyta) and a Male Clone of L. longissima

The cultivation of the first filial generation of monoploid gametophyte clones of different Laminaria species (hybrid Laminaria) is an effective way of utilizing heterozygous vigor (heterosis). A female gametophyte clone of L. japonica and a male gametophyte clone of L. longissima were hybridized, generating Dongfang No.2 hybrid Laminaria. The parentage of this hybrid Laminaria was determined using AFLP of total DNA, SNP of the ITS region of ribosomal RNA transcription unit and microsatellite DNA variation at two loci. In addition to 167 AFLP bands shared by Dongfang No.2, L. japonica and L. longissima, Dongfang No.2 hybrid Laminaria shared another 70 and 55 bands with L. japonica and L. longissima, respectively, which were obviously more than 11 bands shared by L. japonica and L. longissima. Dongfang No.2 held both ‘T’ and ‘C’ at position 847 of the ITS region, while ‘T’ at this position was specific for L. japonica and ‘C’ for L. longissima, respectively. Dongfang No.2 also held the microsatellite DNA alleles of two parents together at two microsatellite DNA marker loci. These observations clearly proved that Dongfang No.2 is a true hybrid of L. japonica and L. longissiuma. Unfortunately, the origin of the chloroplast of Dongfang No.2 was not determined based on the variation of RuBisCo spacer. More sequence variants of both ITS region and RuBisCo spacer were identified in Dongfang No.2 and most of them did not exist in either L. japonica or L. longissima. The unexpected variants may be due to the mutation of ga- metophyte clones occurring during their vegetative amplification.

Outage Probability Minimization for Low-Altitude UAV-Enabled Full-Duplex Mobile Relaying Systems

In this paper, a novel unmanned aerial vehicle(UAV)-enabled full duplex decode-and-forward(DF) technique is used in mobile relaying system. Compared with conventional static relaying, mobile relaying provides more degree of freedom for experiencing better channel conditions and further improving the system reliable performance. The source and relay transmit power as well as relay trajectory are jointly optimized for sum outage probability minimization in mobile relaying system. Due to the non-convex nature of the original problem, block coordinate decent optimization techniques are employed to decompose it into two sub-problems, which leads to an efficient iterative algorithm. Specifically, for the pre-determined relay trajectory, the optimal transmit power of source and relay are obtained by solving Karush-Kuhn-Tucker(KKT) conditions. For the given source/relay power allocation, the optimal UAV trajectory is obtained by solving dual problem. Based on the two steps, an iterative algorithm is proposed to jointly optimize source/relay power allocation and UAV trajectory alternately. Numerical results show the performance gain of our proposed scheme.

Application of LINGO to the Solution of the Water Supply System′s Optimal Operation Model

In this research, LINGO is used successfully to solve the water supply system′s optimal operation model. Firstly, the language of LINGO and the using method were studied intensively, on the basis of which the model was transformed to LINGO form and solved successfully. Secondly, the research on the interface between LINGO and the popular office software was made. The optimization software was developed, which had Excel as the workspace and LINGO as the core of computation. Through practice, this software was found stable, easy to use and suitable for the application to the water supply corporations.

Waveforms analysis and optimization of new electro-hydraulic excitation technology

A new electro-hydraulic exciter that consists of rotary valve and micro-displacement double-functioned hydraulic cylinder was proposed to realize different kinds of waveforms.Calculated fluid dynamics(CFD) simulation of rotary valve orifice reveals that orifice exists the two-throttle phenomenon.According to the finding,the revised flow area model was established.Vibration waveforms analysis was carried out by means of mathematic model and the related experiments were validated.Furthermore,as a new analysis indicator,saturation percentage was introduced first.The experimental results indicate that the revised flow area model is more accurate compared to the original one,and vibration waveforms can be optimized through suitable spool parameters and the revised cylinder structure.

Process Control and Optimization for Heterologous Protein Production by Methylotrophic Pichia pastoris

The methylotrophic yeast Pichia pastoris is a highly successful system for production of a variety of heterologous proteins due to its unique features/abilities for effective protein expression, and tremendous efforts have been made to increase heterologous protein productivity by P. pastoris in recent years. When new engineered yeast strains are constructed and are ready to use tot industrial protein production, process control and optimization techniques should be applied to improve the fermentation performance in the following aspects： （1） increase recombinant cell concentrations in fermentor to high density during growth phase; （2） effectively induce heterologous proteins by enhancing/stabilizing titers or concentrations of the proteins during induction phase; （3） decrease operation costs by relieving the working loads of heat-exchange and oxygen supply. This article reviews and discusses the key and commonly used techniques in heterologous protein production by P. pastoris, with the focus on optimizations of fermentation media and basic operation conditions, development of optimal glycerol feeding strategies for achieving high density cultivation of P. pastoris and effective heterologous protein induction methods by regulating specific growth rate, methanol concentration, temperatures, mixture ratio of multi-carbon substrates, etc. Metabolic analysis for recombinant protein production by P. pastoris is also introduced to interpret the mechanism of sub-optimal heterologous protein production and to explore further optimal expression methods.

Improving Image Copy-Move Forgery Detection with Particle Swarm Optimization Techniques

Copy-Move Forgery(CMF) is one of the simple and effective operations to create forged digital images.Recently,techniques based on Scale Invariant Features Transform(SIFT) are widely used to detect CMF.Various approaches under the SIFT-based framework are the most acceptable ways to CMF detection due to their robust performance.However,for some CMF images,these approaches cannot produce satisfactory detection results.For instance,the number of the matched keypoints may be too less to prove an image to be a CMF image or to generate an accurate result.Sometimes these approaches may even produce error results.According to our observations,one of the reasons is that detection results produced by the SIFT-based framework depend highly on parameters whose values are often determined with experiences.These values are only applicable to a few images,which limits their application.To solve the problem,a novel approach named as CMF Detection with Particle Swarm Optimization(CMFDPSO) is proposed in this paper.CMFD-PSO integrates the Particle Swarm Optimization(PSO) algorithm into the SIFT-based framework.It utilizes the PSO algorithm to generate customized parameter values for images,which are used for CMF detection under the SIFT-based framework.Experimental results show that CMFD-PSO has good performance.