The low-molecular-weight glutenin subunits（LMW-GS）account for 40% of gross glutenins of wheat,usually used as a major indicator for wheat quality improvement.Here,I comprehensively reviewed the advances in LMW-GS fr...The low-molecular-weight glutenin subunits（LMW-GS）account for 40% of gross glutenins of wheat,usually used as a major indicator for wheat quality improvement.Here,I comprehensively reviewed the advances in LMW-GS from their classification,coding gene loci on chromosomes and structural features,polypeptide chains composition,gene sequences accessed in Genbank,molecular markers saturation level,and their impacts on wheat process quality,in order to provide a theoretical basis for the utilization of LMW-GS in genetic improvement of wheat quality.展开更多
Rice with low glutelin content is suitable as functional food for patients affected with diabetes and kidney failure. The fine mapping of the gene(s) responsible for low glutelin content will provide information regar...Rice with low glutelin content is suitable as functional food for patients affected with diabetes and kidney failure. The fine mapping of the gene(s) responsible for low glutelin content will provide information regarding the distribution of glutelin related genes in rice genome and will generate markers for the selection of low glutelin rice varieties. Following an SDS-PAGE screen of rice germplasm from Taihu Valley of China, Japonica selection W3660 is identified to be a novel mutant characterized with low glutelin content. For fine mapping the mutant gene for low glutelin content, F2 and F3 populations were derived from a cross between W3660 and Jingrennuo. SDS-PAGE analysis of the total endosperm protein showed that the low glutelin content trait was controlled by a single dominant nuclear gene. Genetic mapping, using SSRs, located this gene to chromosome 2, in the region between SSR2-001/SSR2-004 and RM1358. The dis- tances of the two markers to the target gene were 1.1 cM and 3.8 cM respectively. By semi-quantitative RT-PCR analysis, the transcripts of GluB4/GluB5 genes located within the region do not change. However, GluB5 gene located proximal to SSR2-001/SSR2-004 was specifically reduced. SSR profiles of seven Japonica varieties were compared with that of W3660 for loci in the relevant genetic region. The markers SSR2-004 and RM1358 were used for marker- assisted selection. The selection efficiencies of SSR2-004 and RM1358 were 96.8% and 92.7% respectively. This provides a standard starting point for the breeding of low glutelin content rice varieties in China.展开更多
Fragile X syndrome （FXS） is one of the most prevalent mental retardations. It is mainly caused by the loss of fragile X mental retardation protein （FMRP）. FMRP is an RNA binding protein and can regulate the transl...Fragile X syndrome （FXS） is one of the most prevalent mental retardations. It is mainly caused by the loss of fragile X mental retardation protein （FMRP）. FMRP is an RNA binding protein and can regulate the translation of its binding RNA, thus regulate several signaling pathways. Many FXS patients show high susceptibility to epilepsy. Epilepsy is a chronic neurological disorder which is characterized by the recurrent appearance of spontaneous seizures due to neuronal hyperactivity in the brain. Both the abnormal activation of several signaling pathway and morphological abnormality that are caused by the loss of FMRP can lead to a high susceptibility to epilepsy. Combining with the research progresses on both FXS and epilepsy, we outlined the possible mechanisms of high susceptibility to epilepsy in FXS and tried to give a prospect on the future research on the mechanism of epilepsy that happened in other mental retardations.展开更多
Where are the zones more enriched in sand deposits in the down slope and deep depression of the low swelling slope belt? Are there any screening conditions for oil and gas there? These are the chief geological problem...Where are the zones more enriched in sand deposits in the down slope and deep depression of the low swelling slope belt? Are there any screening conditions for oil and gas there? These are the chief geological problems to be solved during exploration of a region. Taking the Paleogene system developed along the east slope belt of Chengdao as an example the concepts of sequence stratigraphy and sedimentary sequenc are applied. A new research method likened to a way ''to get a melon by following the vine'' is proposed to determine the direction for exploring within un-drilled or less-drilled areas. This is the process: ''the characteristics of the sequence boundary ? the forming mechanism of the stratigraphic sequence ? the conditions of oil and gas accumulation ? the distribution zones of oil and gas''. The relationship between the dynamic mechanism of stratigraphic sequence and the forming conditions for oil and gas accumulation establishes that the tectonic disturbance of the slope belt has significant responses as denudation and deposition. Above the stratigraphic sequence boundary there are large scale sand bodies of the low stand system tract (LST) that have developed in the low swelling slope belt and its deep depression. Below the sequence boundary there are the remaining sand bodies of the high stand system tract (HST). On the slope there is a convergence of mudstone layers of the extended system tract (EST) with the mudstone of the underlying strata, which constitutes the screening conditions for the reservoir of the down slope and deep depression. The distribution regularities in preferred sand bodies on the surface of the sequence boundary, and in the system tract, indicate the ordering of oil-gas deposits. From the higher stand down to the depth of the slope there are, in order, areas where exploration was unfavorable, major areas of stratigraphic overlap of oil-gas reservoirs, unconformity screened oil-gas reservoirs, and, finally, sandstone lens oil-gas reservoirs. The low swelling slope belt of Chengdao was tectonically active, which is typical for a continental rift basin. The methodology and results of the present paper are significant for the theory and practice of predicting subtle reservoir and selecting strategic areas for exploration.展开更多
The acronym C6 means ＂Climatic Changes and Carbon Cycle in Canyons and Caves＂. It is a monitoring project, for the evaluation of climate change signals, based on measuring sites located inside canyons and caves; it ...The acronym C6 means ＂Climatic Changes and Carbon Cycle in Canyons and Caves＂. It is a monitoring project, for the evaluation of climate change signals, based on measuring sites located inside canyons and caves; it merged in the year 2005, under the scientific supervision of the Palermo Branch of the Italian National Institute for Geophysics and Volcanology （INGV）, two different monitoring programs active since 1999. The choice of these environments is based on their morphological structure： being them more or less segregated respect the outer atmosphere, they act as low-pass filters respect the variations of the monitored parameters, which are rainfall and dropping water amounts and rates, air temperatures and relative humidity and carbon dioxide concentrations in the atmosphere. On the basis of the preliminary data, reported and discussed in the paper, the C6 network seems to be capable to give useful information on the local effects of global changes, even if at the moment the monitored parameters concern only the abiotic components of the studied ecosystems.展开更多
Cadmium(Cd) is an element that is nonessential and extremely toxic to both plants and human beings. Soil contaminated with Cd has adverse impacts on crop yields and threatens human health via the food chain. Cultivati...Cadmium(Cd) is an element that is nonessential and extremely toxic to both plants and human beings. Soil contaminated with Cd has adverse impacts on crop yields and threatens human health via the food chain. Cultivation of low-Cd cultivars has been of particular interest and is one of the most cost-effective and promising approaches to minimize human dietary intake of Cd. Low-Cd crop cultivars should meet particular criteria, including acceptable yield and quality, and their edible parts should have Cd concentrations below maximum permissible concentrations for safe consumption, even when grown in Cd-contaminated soil. Several low-Cd cereal cultivars and genotypes have been developed worldwide through cultivar screening and conventional breeding. Molecular markers are powerful in facilitating the selection of low-Cd cereal cultivars. Modern molecular breeding technologies may have great potential in breeding programs for the development of low-Cd cultivars, especially when coupled with conventional breeding. In this review, we provide a synthesis of low-Cd cereal breeding.展开更多
Based on multiple types of data, the topographical features of the Okinawa Trough(OT) have been characterized and a computation method has been proposed to determine the break point of continental shelf(BOS), foot poi...Based on multiple types of data, the topographical features of the Okinawa Trough(OT) have been characterized and a computation method has been proposed to determine the break point of continental shelf(BOS), foot point of the continental slope(FOS), the central axial point, and the maximum depth point. A total of 48 topographical profiles that crosscut the continental slope have been used to determine the trends of the BOS and FOS(the BOS and FOS lines) in the East China Sea(ECS). The trend of central axial points in the OT has been similarly determined by analyzing 39 topographical profiles across the axis of the trough. The BOS line forms the boundary between the continental shelf and slope. In the ECS, the BOS line roughly follows the 200 m isobath, continuously in the northern and middle parts of the OT, but jumping about somewhat in the south. The FOS line is the boundary between the continental slope and the bottom of the trough. The depth of the FOS increases gradually from north to south in the OT. Intense incisions by canyons into the slope in the southern part of the trough have led to the complex distribution of FOS. Topographical profiles crosscutting the northern, middle, and southern parts of the OT exhibit features that include: a single W-shape, a composite W-shape, and a U-shape, respectively, which suggests that in the middle and northern parts of the trough the central axial points are always located on seamount peaks or ridges associated with linear seamounts, whereas in the south they are found in the center of en echelon depressions. The line formed by the central axial points is the east-west dividing line of the OT, which indicates that the trough is a natural gap that prevents the extension of ECS continental shelf to the east. The distributions of the BOS and FOS lines are influenced by fluctuation of sea levels and submarine canyons, whereas the distribution of axis lines is controlled by tectonics and deposition.展开更多
文摘The low-molecular-weight glutenin subunits（LMW-GS）account for 40% of gross glutenins of wheat,usually used as a major indicator for wheat quality improvement.Here,I comprehensively reviewed the advances in LMW-GS from their classification,coding gene loci on chromosomes and structural features,polypeptide chains composition,gene sequences accessed in Genbank,molecular markers saturation level,and their impacts on wheat process quality,in order to provide a theoretical basis for the utilization of LMW-GS in genetic improvement of wheat quality.
基金supported by the grants from Hi-Tech Research and Development Program of China("863"Program,No.2003AA222131,2003AA207020)the National Natural Science Foundation of China(No.30170570)Special Program for gene-transfering(No.JY03-B-07,JY03-A-07-02)
文摘Rice with low glutelin content is suitable as functional food for patients affected with diabetes and kidney failure. The fine mapping of the gene(s) responsible for low glutelin content will provide information regarding the distribution of glutelin related genes in rice genome and will generate markers for the selection of low glutelin rice varieties. Following an SDS-PAGE screen of rice germplasm from Taihu Valley of China, Japonica selection W3660 is identified to be a novel mutant characterized with low glutelin content. For fine mapping the mutant gene for low glutelin content, F2 and F3 populations were derived from a cross between W3660 and Jingrennuo. SDS-PAGE analysis of the total endosperm protein showed that the low glutelin content trait was controlled by a single dominant nuclear gene. Genetic mapping, using SSRs, located this gene to chromosome 2, in the region between SSR2-001/SSR2-004 and RM1358. The dis- tances of the two markers to the target gene were 1.1 cM and 3.8 cM respectively. By semi-quantitative RT-PCR analysis, the transcripts of GluB4/GluB5 genes located within the region do not change. However, GluB5 gene located proximal to SSR2-001/SSR2-004 was specifically reduced. SSR profiles of seven Japonica varieties were compared with that of W3660 for loci in the relevant genetic region. The markers SSR2-004 and RM1358 were used for marker- assisted selection. The selection efficiencies of SSR2-004 and RM1358 were 96.8% and 92.7% respectively. This provides a standard starting point for the breeding of low glutelin content rice varieties in China.
文摘Fragile X syndrome （FXS） is one of the most prevalent mental retardations. It is mainly caused by the loss of fragile X mental retardation protein （FMRP）. FMRP is an RNA binding protein and can regulate the translation of its binding RNA, thus regulate several signaling pathways. Many FXS patients show high susceptibility to epilepsy. Epilepsy is a chronic neurological disorder which is characterized by the recurrent appearance of spontaneous seizures due to neuronal hyperactivity in the brain. Both the abnormal activation of several signaling pathway and morphological abnormality that are caused by the loss of FMRP can lead to a high susceptibility to epilepsy. Combining with the research progresses on both FXS and epilepsy, we outlined the possible mechanisms of high susceptibility to epilepsy in FXS and tried to give a prospect on the future research on the mechanism of epilepsy that happened in other mental retardations.
基金The present paper is a part of the research work of the post-doc program: ''The Research on the Genesis of Paleogene Sand Bodies in the Chengdao East Slope and Its Controlling Factors'' (No.YKB 0804)
文摘Where are the zones more enriched in sand deposits in the down slope and deep depression of the low swelling slope belt? Are there any screening conditions for oil and gas there? These are the chief geological problems to be solved during exploration of a region. Taking the Paleogene system developed along the east slope belt of Chengdao as an example the concepts of sequence stratigraphy and sedimentary sequenc are applied. A new research method likened to a way ''to get a melon by following the vine'' is proposed to determine the direction for exploring within un-drilled or less-drilled areas. This is the process: ''the characteristics of the sequence boundary ? the forming mechanism of the stratigraphic sequence ? the conditions of oil and gas accumulation ? the distribution zones of oil and gas''. The relationship between the dynamic mechanism of stratigraphic sequence and the forming conditions for oil and gas accumulation establishes that the tectonic disturbance of the slope belt has significant responses as denudation and deposition. Above the stratigraphic sequence boundary there are large scale sand bodies of the low stand system tract (LST) that have developed in the low swelling slope belt and its deep depression. Below the sequence boundary there are the remaining sand bodies of the high stand system tract (HST). On the slope there is a convergence of mudstone layers of the extended system tract (EST) with the mudstone of the underlying strata, which constitutes the screening conditions for the reservoir of the down slope and deep depression. The distribution regularities in preferred sand bodies on the surface of the sequence boundary, and in the system tract, indicate the ordering of oil-gas deposits. From the higher stand down to the depth of the slope there are, in order, areas where exploration was unfavorable, major areas of stratigraphic overlap of oil-gas reservoirs, unconformity screened oil-gas reservoirs, and, finally, sandstone lens oil-gas reservoirs. The low swelling slope belt of Chengdao was tectonically active, which is typical for a continental rift basin. The methodology and results of the present paper are significant for the theory and practice of predicting subtle reservoir and selecting strategic areas for exploration.
文摘The acronym C6 means ＂Climatic Changes and Carbon Cycle in Canyons and Caves＂. It is a monitoring project, for the evaluation of climate change signals, based on measuring sites located inside canyons and caves; it merged in the year 2005, under the scientific supervision of the Palermo Branch of the Italian National Institute for Geophysics and Volcanology （INGV）, two different monitoring programs active since 1999. The choice of these environments is based on their morphological structure： being them more or less segregated respect the outer atmosphere, they act as low-pass filters respect the variations of the monitored parameters, which are rainfall and dropping water amounts and rates, air temperatures and relative humidity and carbon dioxide concentrations in the atmosphere. On the basis of the preliminary data, reported and discussed in the paper, the C6 network seems to be capable to give useful information on the local effects of global changes, even if at the moment the monitored parameters concern only the abiotic components of the studied ecosystems.
基金Project supported by the Key Research Foundation of Science and Technology Department of Zhejiang Province of China(No.2016C02050-9-7)。
文摘Cadmium(Cd) is an element that is nonessential and extremely toxic to both plants and human beings. Soil contaminated with Cd has adverse impacts on crop yields and threatens human health via the food chain. Cultivation of low-Cd cultivars has been of particular interest and is one of the most cost-effective and promising approaches to minimize human dietary intake of Cd. Low-Cd crop cultivars should meet particular criteria, including acceptable yield and quality, and their edible parts should have Cd concentrations below maximum permissible concentrations for safe consumption, even when grown in Cd-contaminated soil. Several low-Cd cereal cultivars and genotypes have been developed worldwide through cultivar screening and conventional breeding. Molecular markers are powerful in facilitating the selection of low-Cd cereal cultivars. Modern molecular breeding technologies may have great potential in breeding programs for the development of low-Cd cultivars, especially when coupled with conventional breeding. In this review, we provide a synthesis of low-Cd cereal breeding.
基金supported by Public Science and Technology Research Funds Project of Ocean(Grant No.201105001)Fundamental Project of Science and Technology(Grant No.2013FY112900)National Natural Science Foundation of China(Grant Nos.40506017,41206046)
文摘Based on multiple types of data, the topographical features of the Okinawa Trough(OT) have been characterized and a computation method has been proposed to determine the break point of continental shelf(BOS), foot point of the continental slope(FOS), the central axial point, and the maximum depth point. A total of 48 topographical profiles that crosscut the continental slope have been used to determine the trends of the BOS and FOS(the BOS and FOS lines) in the East China Sea(ECS). The trend of central axial points in the OT has been similarly determined by analyzing 39 topographical profiles across the axis of the trough. The BOS line forms the boundary between the continental shelf and slope. In the ECS, the BOS line roughly follows the 200 m isobath, continuously in the northern and middle parts of the OT, but jumping about somewhat in the south. The FOS line is the boundary between the continental slope and the bottom of the trough. The depth of the FOS increases gradually from north to south in the OT. Intense incisions by canyons into the slope in the southern part of the trough have led to the complex distribution of FOS. Topographical profiles crosscutting the northern, middle, and southern parts of the OT exhibit features that include: a single W-shape, a composite W-shape, and a U-shape, respectively, which suggests that in the middle and northern parts of the trough the central axial points are always located on seamount peaks or ridges associated with linear seamounts, whereas in the south they are found in the center of en echelon depressions. The line formed by the central axial points is the east-west dividing line of the OT, which indicates that the trough is a natural gap that prevents the extension of ECS continental shelf to the east. The distributions of the BOS and FOS lines are influenced by fluctuation of sea levels and submarine canyons, whereas the distribution of axis lines is controlled by tectonics and deposition.
