In the present study, we, for the first time, developed an UPLC-IT-TOF/MS method to compare the chemical constituents of the flower buds of Lonicera japonica Thunb. （Jinyinhua, JYH, in Chinese） and its tetraploid cu...In the present study, we, for the first time, developed an UPLC-IT-TOF/MS method to compare the chemical constituents of the flower buds of Lonicera japonica Thunb. （Jinyinhua, JYH, in Chinese） and its tetraploid cultivar Jiufengyihao （JFYH）. The samples were separated on a C18 ultra-column （100 mm×2.1 ram, 1.8 μm） eluted with a mixture of acetonitrile and 0.1% formic acid using a gradient program. Based on the accurate masses, fragmental MS2 ions and fragmentation pathway, we unambiguously affirmed or tentatively identified 30 compounds, including 11 phenolic acids, 10 iridoid glycosides, 8 fiavonoids and 1 saponin, from JYH and JFYH. Among them, all compounds were detected in Lonicerajaponica, but only 21 compounds were detected in JFYH. In addition, the relative contents of the 21 common chemical constituents were also different in JYH and JFYH, suggesting an obvious chemical difference between JYH and JFYH.展开更多
文摘In the present study, we, for the first time, developed an UPLC-IT-TOF/MS method to compare the chemical constituents of the flower buds of Lonicera japonica Thunb. （Jinyinhua, JYH, in Chinese） and its tetraploid cultivar Jiufengyihao （JFYH）. The samples were separated on a C18 ultra-column （100 mm×2.1 ram, 1.8 μm） eluted with a mixture of acetonitrile and 0.1% formic acid using a gradient program. Based on the accurate masses, fragmental MS2 ions and fragmentation pathway, we unambiguously affirmed or tentatively identified 30 compounds, including 11 phenolic acids, 10 iridoid glycosides, 8 fiavonoids and 1 saponin, from JYH and JFYH. Among them, all compounds were detected in Lonicerajaponica, but only 21 compounds were detected in JFYH. In addition, the relative contents of the 21 common chemical constituents were also different in JYH and JFYH, suggesting an obvious chemical difference between JYH and JFYH.
