[Objective] Mononychellus tanajoa is a mite speices recently invaded into China in 2008. Temperature is one of the most important ecological factors affecting the growth and reproduction of M. tanajoa. The objective o...[Objective] Mononychellus tanajoa is a mite speices recently invaded into China in 2008. Temperature is one of the most important ecological factors affecting the growth and reproduction of M. tanajoa. The objective of the current study was to reveal the effects of high temperature incubation on the activities of some protective enzymes in M. tanajoa at different growth stages. The results would contribute to the understanding of the adaptable distribution of M. tanajoa after its invasion into China, the mechanisms in its invasion, diffusion and ecological adaptation, and the monitor- ing, early warning and effective prevention of its damage. [Method] Six protective enzymes, Le. polyphenol oxidase (PPO), peroxidase (POD), ascorbate oxidase (AsA- POD), catalase (CAT), superoxide dismutase (SOD) and esterase (EST), were cho- sen to study their activities after the mites at different growth stages were incubated at a extremely high temperature of 42 ~C for a certain period of time up to 24 h. The activities were measured by spectrophotometric endpoint assay method. [Results] Enzyme activities in M. tanajoa were affected by the high temperature incubation. However, differences in enzyme activity changes were found among different protec- tive enzymes and among different growth stages of M. tanajoa. Activities of PPO, POD, AsA-POD and CAT were significantly increased in the larval mites and female adult mites of M. tanajoa. CAT activity was significantly decreased in protonymph and deutonymph of M. tanajoa. Activities of PPO, POD and AsA-POD in protonymph and deutonymph showed no obvious difference from the control. [Conclusion] The activity changes of some protective enzymes in M. tanajoa following high-temperature treatment are part of its anti-stress reaction mechanism. In mite protonymph and deutonymph, activities of PPO, POD and AsA-POD are similar to the untreated con- trol which may be associated with the thermostability of M. tanajoa. It is concluded that, the long-time stress of extreme temperature may result in the increase of the thermostability of mite individuals, the enhancement of the population thermal stability and subsequently lead to rapid expansion of the population.展开更多
AIM:To investigate the effect of age on severity of acute pancreatitis(AP) using biochemical markers,histology and expression of the protective pancreatitisassociated proteins(PAPs).METHODS:AP was induced via intraduc...AIM:To investigate the effect of age on severity of acute pancreatitis(AP) using biochemical markers,histology and expression of the protective pancreatitisassociated proteins(PAPs).METHODS:AP was induced via intraductal injection of 4% sodium taurocholate in young and old rats.Sera and pancreata were assayed at 24 h for the parameters listed above;we also employed a novel molecular technique to assess bacterial infiltration using polymerase chain reaction to measure bacterial genomic ribosomal RNA.RESULTS:At 24 h after induction of AP,the pancreata of older animals had less edema(mean ± SE histologic score of young vs old:3.11 ± 0.16 vs 2.50 ±-0.11,P < 0.05),decreased local inflammatory response(histologic score of stromal infiltrate:3.11 ± 0.27 vs 2.00 ± 0.17,P < 0.05) and increased bacterial infiltration(174% ± 52% increase from sham vs 377% ± 4%,P < 0.05).A decreased expression of PAP1 and PAP2 was demonstrated by Western blotting analysis and immunohistochemical staining.There were no differences in serum amylase and lipase activity,or tissue myeloperoxidase or monocyte chemotactic protein-1 levels.However,in the most-aged group,serum C-reactive protein levels were higher(young vs old:0.249 ± 0.04 mg/dL vs 2.45 ± 0.68 mg/dL,P < 0.05).CONCLUSION:In older animals,there is depressed PAP expression related to a blunted inflammatory response in AP which is associated with worsened bacterial infiltration and higher C-reactive protein level;this may explain the more aggressive clinical course.展开更多
SPSS13.0 was used to process the data of the 1-6 batch of key cultural relic conservation units promulgated by the State Administration of Cultural Heritage of China;and multi-element evaluation method was used to cal...SPSS13.0 was used to process the data of the 1-6 batch of key cultural relic conservation units promulgated by the State Administration of Cultural Heritage of China;and multi-element evaluation method was used to calculate the composite distribution index.The spatial distribution of cultural relics of provinces in China can be divided into four grades,namely:very rich areas,rich areas,poor areas and very poor areas;the article analyzes the characteristics of cultural resources in spatial and temporal distribution.The paper holds that(1)the distribution of China's cultural relic tourism resources is relatively concentrated,different sharply from region to region,with more Han nation's heritage,less relics of the ethnic minorities;(2) Henan,Hebei,Shaanxi,Shanxi Province are the main regions with heavy distribution of cultural resources;(3) relics appeared in specific time periods in China's history.Ming and Qing Dynasties,Sui,Tang and Five Dynasties,Song,Liao and Jin,the Western Han,Shang and Zhou dynasties,the New Stone Age are the major periods producing more cultural relics.This paper also analyzes relationships of the emergence of cultural relics with production technology,specific historical process,specific geographical location,political system,religion,national culture,customs,architectural styles and techniques of the traditional cultural educational system.Finally,the paper discusses the development and protection of heritage.展开更多
AIM:To investigate the effect of administration of agmatine(AGM) on gastric protection against ischemia reperfusion(I/R) injury.METHODS:Three groups of rats(6/group);sham,gastric I/R injury,and gastric I/R + AGM(100 m...AIM:To investigate the effect of administration of agmatine(AGM) on gastric protection against ischemia reperfusion(I/R) injury.METHODS:Three groups of rats(6/group);sham,gastric I/R injury,and gastric I/R + AGM(100 mg/kg,i.p.given 15 min prior to gastric ischemia) were recruited.Gastric injury was conducted by ligating celiac artery for 30 min and reperfusion for another 30 min.Gastric tissues were histologically studied and immunostained with angiopoietin 1(Ang-1) and Ang-2.Vascular endothelial growth factor(VEGF) and monocyte chemoattractant protein-1(MCP-1) were measured in gastric tissue homogenate.To assess whether AKt/phosphatidyl inositol-3-kinase(PI3K) mediated the effect of AGM,an additional group was pretreated with Wortmannin(WM)(inhibitor of Akt/PI3K,15 μg/kg,i.p.),prior to ischemic injury and AGM treatment,and examined histologically and immunostained.Another set of experiments was run to study vascular permeability of the stomach using Evan's blue dye.RESULTS:AGM markedly reduced Evan's blue dye extravasation(3.58 ± 0.975 μg/stomach vs 1.175 ± 0.374 μg/stomach,P < 0.05),VEGF(36.87 ± 2.71 pg/100 mg protein vs 48.4 ± 6.53 pg/100 mg protein,P < 0.05) and MCP-1 tissue level(29.5 ± 7 pg/100 mg protein vs 41.17 ± 10.4 pg/100 mg protein,P < 0.01).It preserved gastric histology and reduced congestion.Ang-1 and Ang-2 immunostaining were reduced in stomach sections of AGM-treated animals.The administration of WM abolished the protective effects of AGM and extensive hemorrhage and ulcerations were seen.CONCLUSION:AGM protects the stomach against I/R injury by reducing vascular permeability and inflammation.This protection is possibly mediated by Akt/PI3K.展开更多
Research on the evolution of cultural policy is the key in the field of public policy study,and it is also an important means to solve the problems that have emerged in cultural heritage management.The National Cultur...Research on the evolution of cultural policy is the key in the field of public policy study,and it is also an important means to solve the problems that have emerged in cultural heritage management.The National Cultural Park is an innovative cultural heritage protection system proposed by Chinese leaders within the context of national rejuvenation and building a culturally-strong country campaign.From the perspective of system evolution theory,this paper systematically sorts out the ideas behind international heritage management and the evolutionary process of the Cultural Relics Protection Unit system,in an effort to explore the origin,innovation and vision of the National Cultural Park System.A review of the international heritage documents revealed that the principle of heritage protection has developed from“authenticity”to“integrity”,and the focus of the protection object has also changed from“monism”to“diversity”,which provided a theoretical background for the burgeoning National Cultural Park.The Cultural Relics Protection Unit system has been promoted as the most crucial cultural heritage management system in China.Therefore,this study sheds light on the evolution and limitations of the system that lays a practical foundation for the National Cultural Park System.There have been three stages in the history of China’s National Cultural Relics Protection Unit,namely,the creation of the system(1956–1965),the survival crisis of the system(1966–1977),and the rebirth and development of the system(1978 to the present),in which the main driving concepts of China's cultural heritage management have been sequentially elucidated as simple protectionism,tolerant conservatism and comprehensive developmentalism,respectively.Since the establishment of China’s Cultural Relics Protection Unit system,tremendous progress has been made not only in the enlightenment of the public’s mindset and but also in the development of system and culture.However,the changes in the institutional environment gradually exposed its limitations regarding cultural relic management and value expression.The National Cultural Park System is the fruit created by the internal contradictions of the cultural heritage system itself and the evolution of heritage theory.Its biggest innovation lies in the two core connotations of“integrated protection”and“integrated development”.In the future,as the National Cultural Park management system matures,a Chinese cultural heritage management system will take shape in which the National Cultural Parks become dominant and various cultural resources are utilized.Finally,the National Cultural Park will become a symbol of Chinese culture and will be internalized into the common belief system of the Chinese nation.展开更多
Physical activity can enhance cognitive function and increase resistance against deleterious effects of stress on mental health. Enhanced cognitive function and stress resistance produced by exercise are conserved amo...Physical activity can enhance cognitive function and increase resistance against deleterious effects of stress on mental health. Enhanced cognitive function and stress resistance produced by exercise are conserved among vertebrates, suggesting that ubiquitous mechanisms may underlie beneficial ef- fects of exercise. In the current review, we summarize the beneficial effects of exercise on cognitive function and stress resistance and discuss central and peripheral signaling factors that may be critical for conferring the effects of physical activity to brain circuits involved in cognitive function and stress. Additionally, it is suggested that norepinephrine and serotonin, highly conserved monoamines that are sensitive to exercise and able to modulate behavior in multiple species, could represent a conver- gence between peripheral and central exercise signals that mediate the beneficial effects of exercise. Finally, we offer the novel hypothesis that thermoregulation during exercise could contribute to the emotional effects of exercise by activating a subset of temperature-sensitive serotonergic neurons in the dorsal raphe nucleus that convey anxiolytic and stress-protective signals to forebrain regions. Throughout the review, we discuss limitations to current approaches and offer strategies for future re- search in exercise neuroscience.展开更多
The randomized response (RR) technique is an effective survey method when collecting sensitive information. This paper proposes a new non-randomized response model for survey sampling with polychotomous sensitive qu...The randomized response (RR) technique is an effective survey method when collecting sensitive information. This paper proposes a new non-randomized response model for survey sampling with polychotomous sensitive questions: Two-valued response technique. The proportion of the sensitive attribute and its estimator's variance are estimated under the simple random sampling without replacement and with replacement designs. The relation between the efficiency and the protection of privacy is discussed. The result indicates that the efficiency is in conflict with protection of privacy in the new proposed model, which is the same as that in the RR technique, but the new technique has better characteristics in sociological practice aspects than the RR technique, because it is of low cost, saving time and being operated easily.展开更多
基金Supported by Special Fund for Cassava Technology System Fund (CARS-12-hncq)the Central-level Public Welfare Research Institutes for Basic R & D Operations (No.2011h-zs1J014,No.2009hzs1J013)+1 种基金Agricultural Public Welfare Industry-specific (200903034-5)Science and Technology Program Project of Hainan Province (ZDXM20100022,ZDXM20110032)~~
文摘[Objective] Mononychellus tanajoa is a mite speices recently invaded into China in 2008. Temperature is one of the most important ecological factors affecting the growth and reproduction of M. tanajoa. The objective of the current study was to reveal the effects of high temperature incubation on the activities of some protective enzymes in M. tanajoa at different growth stages. The results would contribute to the understanding of the adaptable distribution of M. tanajoa after its invasion into China, the mechanisms in its invasion, diffusion and ecological adaptation, and the monitor- ing, early warning and effective prevention of its damage. [Method] Six protective enzymes, Le. polyphenol oxidase (PPO), peroxidase (POD), ascorbate oxidase (AsA- POD), catalase (CAT), superoxide dismutase (SOD) and esterase (EST), were cho- sen to study their activities after the mites at different growth stages were incubated at a extremely high temperature of 42 ~C for a certain period of time up to 24 h. The activities were measured by spectrophotometric endpoint assay method. [Results] Enzyme activities in M. tanajoa were affected by the high temperature incubation. However, differences in enzyme activity changes were found among different protec- tive enzymes and among different growth stages of M. tanajoa. Activities of PPO, POD, AsA-POD and CAT were significantly increased in the larval mites and female adult mites of M. tanajoa. CAT activity was significantly decreased in protonymph and deutonymph of M. tanajoa. Activities of PPO, POD and AsA-POD in protonymph and deutonymph showed no obvious difference from the control. [Conclusion] The activity changes of some protective enzymes in M. tanajoa following high-temperature treatment are part of its anti-stress reaction mechanism. In mite protonymph and deutonymph, activities of PPO, POD and AsA-POD are similar to the untreated con- trol which may be associated with the thermostability of M. tanajoa. It is concluded that, the long-time stress of extreme temperature may result in the increase of the thermostability of mite individuals, the enhancement of the population thermal stability and subsequently lead to rapid expansion of the population.
文摘AIM:To investigate the effect of age on severity of acute pancreatitis(AP) using biochemical markers,histology and expression of the protective pancreatitisassociated proteins(PAPs).METHODS:AP was induced via intraductal injection of 4% sodium taurocholate in young and old rats.Sera and pancreata were assayed at 24 h for the parameters listed above;we also employed a novel molecular technique to assess bacterial infiltration using polymerase chain reaction to measure bacterial genomic ribosomal RNA.RESULTS:At 24 h after induction of AP,the pancreata of older animals had less edema(mean ± SE histologic score of young vs old:3.11 ± 0.16 vs 2.50 ±-0.11,P < 0.05),decreased local inflammatory response(histologic score of stromal infiltrate:3.11 ± 0.27 vs 2.00 ± 0.17,P < 0.05) and increased bacterial infiltration(174% ± 52% increase from sham vs 377% ± 4%,P < 0.05).A decreased expression of PAP1 and PAP2 was demonstrated by Western blotting analysis and immunohistochemical staining.There were no differences in serum amylase and lipase activity,or tissue myeloperoxidase or monocyte chemotactic protein-1 levels.However,in the most-aged group,serum C-reactive protein levels were higher(young vs old:0.249 ± 0.04 mg/dL vs 2.45 ± 0.68 mg/dL,P < 0.05).CONCLUSION:In older animals,there is depressed PAP expression related to a blunted inflammatory response in AP which is associated with worsened bacterial infiltration and higher C-reactive protein level;this may explain the more aggressive clinical course.
基金supported by Science Research Program of Hainan Provincial Education Bureau (Grant No Hj2008-79)funded by the National Natural Science Foundation of China (Grant Nos 40963001, 40061003, 40661013)Key Course Program of Natural Geography of Hainan Normal University and First-degree Course Program of Geographical Science of Hainan Province
文摘SPSS13.0 was used to process the data of the 1-6 batch of key cultural relic conservation units promulgated by the State Administration of Cultural Heritage of China;and multi-element evaluation method was used to calculate the composite distribution index.The spatial distribution of cultural relics of provinces in China can be divided into four grades,namely:very rich areas,rich areas,poor areas and very poor areas;the article analyzes the characteristics of cultural resources in spatial and temporal distribution.The paper holds that(1)the distribution of China's cultural relic tourism resources is relatively concentrated,different sharply from region to region,with more Han nation's heritage,less relics of the ethnic minorities;(2) Henan,Hebei,Shaanxi,Shanxi Province are the main regions with heavy distribution of cultural resources;(3) relics appeared in specific time periods in China's history.Ming and Qing Dynasties,Sui,Tang and Five Dynasties,Song,Liao and Jin,the Western Han,Shang and Zhou dynasties,the New Stone Age are the major periods producing more cultural relics.This paper also analyzes relationships of the emergence of cultural relics with production technology,specific historical process,specific geographical location,political system,religion,national culture,customs,architectural styles and techniques of the traditional cultural educational system.Finally,the paper discusses the development and protection of heritage.
基金Supported by The Deanship of Scientific Research at King Saud University,through research group project,No.RGPVPP-016,entitled "Cardiovascular research Group"
文摘AIM:To investigate the effect of administration of agmatine(AGM) on gastric protection against ischemia reperfusion(I/R) injury.METHODS:Three groups of rats(6/group);sham,gastric I/R injury,and gastric I/R + AGM(100 mg/kg,i.p.given 15 min prior to gastric ischemia) were recruited.Gastric injury was conducted by ligating celiac artery for 30 min and reperfusion for another 30 min.Gastric tissues were histologically studied and immunostained with angiopoietin 1(Ang-1) and Ang-2.Vascular endothelial growth factor(VEGF) and monocyte chemoattractant protein-1(MCP-1) were measured in gastric tissue homogenate.To assess whether AKt/phosphatidyl inositol-3-kinase(PI3K) mediated the effect of AGM,an additional group was pretreated with Wortmannin(WM)(inhibitor of Akt/PI3K,15 μg/kg,i.p.),prior to ischemic injury and AGM treatment,and examined histologically and immunostained.Another set of experiments was run to study vascular permeability of the stomach using Evan's blue dye.RESULTS:AGM markedly reduced Evan's blue dye extravasation(3.58 ± 0.975 μg/stomach vs 1.175 ± 0.374 μg/stomach,P < 0.05),VEGF(36.87 ± 2.71 pg/100 mg protein vs 48.4 ± 6.53 pg/100 mg protein,P < 0.05) and MCP-1 tissue level(29.5 ± 7 pg/100 mg protein vs 41.17 ± 10.4 pg/100 mg protein,P < 0.01).It preserved gastric histology and reduced congestion.Ang-1 and Ang-2 immunostaining were reduced in stomach sections of AGM-treated animals.The administration of WM abolished the protective effects of AGM and extensive hemorrhage and ulcerations were seen.CONCLUSION:AGM protects the stomach against I/R injury by reducing vascular permeability and inflammation.This protection is possibly mediated by Akt/PI3K.
基金The China National Social Science Fund of Art Sciences(20ZD02).
文摘Research on the evolution of cultural policy is the key in the field of public policy study,and it is also an important means to solve the problems that have emerged in cultural heritage management.The National Cultural Park is an innovative cultural heritage protection system proposed by Chinese leaders within the context of national rejuvenation and building a culturally-strong country campaign.From the perspective of system evolution theory,this paper systematically sorts out the ideas behind international heritage management and the evolutionary process of the Cultural Relics Protection Unit system,in an effort to explore the origin,innovation and vision of the National Cultural Park System.A review of the international heritage documents revealed that the principle of heritage protection has developed from“authenticity”to“integrity”,and the focus of the protection object has also changed from“monism”to“diversity”,which provided a theoretical background for the burgeoning National Cultural Park.The Cultural Relics Protection Unit system has been promoted as the most crucial cultural heritage management system in China.Therefore,this study sheds light on the evolution and limitations of the system that lays a practical foundation for the National Cultural Park System.There have been three stages in the history of China’s National Cultural Relics Protection Unit,namely,the creation of the system(1956–1965),the survival crisis of the system(1966–1977),and the rebirth and development of the system(1978 to the present),in which the main driving concepts of China's cultural heritage management have been sequentially elucidated as simple protectionism,tolerant conservatism and comprehensive developmentalism,respectively.Since the establishment of China’s Cultural Relics Protection Unit system,tremendous progress has been made not only in the enlightenment of the public’s mindset and but also in the development of system and culture.However,the changes in the institutional environment gradually exposed its limitations regarding cultural relic management and value expression.The National Cultural Park System is the fruit created by the internal contradictions of the cultural heritage system itself and the evolution of heritage theory.Its biggest innovation lies in the two core connotations of“integrated protection”and“integrated development”.In the future,as the National Cultural Park management system matures,a Chinese cultural heritage management system will take shape in which the National Cultural Parks become dominant and various cultural resources are utilized.Finally,the National Cultural Park will become a symbol of Chinese culture and will be internalized into the common belief system of the Chinese nation.
文摘Physical activity can enhance cognitive function and increase resistance against deleterious effects of stress on mental health. Enhanced cognitive function and stress resistance produced by exercise are conserved among vertebrates, suggesting that ubiquitous mechanisms may underlie beneficial ef- fects of exercise. In the current review, we summarize the beneficial effects of exercise on cognitive function and stress resistance and discuss central and peripheral signaling factors that may be critical for conferring the effects of physical activity to brain circuits involved in cognitive function and stress. Additionally, it is suggested that norepinephrine and serotonin, highly conserved monoamines that are sensitive to exercise and able to modulate behavior in multiple species, could represent a conver- gence between peripheral and central exercise signals that mediate the beneficial effects of exercise. Finally, we offer the novel hypothesis that thermoregulation during exercise could contribute to the emotional effects of exercise by activating a subset of temperature-sensitive serotonergic neurons in the dorsal raphe nucleus that convey anxiolytic and stress-protective signals to forebrain regions. Throughout the review, we discuss limitations to current approaches and offer strategies for future re- search in exercise neuroscience.
基金This research is supported by the National Natural Science Foundation of China under Grant No. 10761004, the Natural Science Foundation of Inner Mongolia under Grant Nos. 2009MS0107 and 2010MS01116, the Talent Development Foundation of Inner Mongolia (2007), and the Natural Science Foundation of Inner Mongolia University of Technology under Grant No. X200934.
文摘The randomized response (RR) technique is an effective survey method when collecting sensitive information. This paper proposes a new non-randomized response model for survey sampling with polychotomous sensitive questions: Two-valued response technique. The proportion of the sensitive attribute and its estimator's variance are estimated under the simple random sampling without replacement and with replacement designs. The relation between the efficiency and the protection of privacy is discussed. The result indicates that the efficiency is in conflict with protection of privacy in the new proposed model, which is the same as that in the RR technique, but the new technique has better characteristics in sociological practice aspects than the RR technique, because it is of low cost, saving time and being operated easily.