15-day old seedlings of wheat and rape were grown in a series of solutions with different concentrations of KNO3 for a definite period of time. The changes in NO3- concentration of the solutions were determined by the...15-day old seedlings of wheat and rape were grown in a series of solutions with different concentrations of KNO3 for a definite period of time. The changes in NO3- concentration of the solutions were determined by the double ion-selective electrode method, and then the amount of NO3- taken up by the plants was estimated and values of Km and Imax of the Michealis-Menten equation were calculated. Results show that both the method and conditions of determination affected the values of Km and Imax. For example, the Km value was appreciably reduced when the volume of culture solution was increased or when the duration of nutrient uptake was shortened; the Km value obtained with short-term depletion method was higher than that obtained with long-term one. Similar Variations were found for the values of Imax. There was a considerable difference in the characteristics of uptake kinetics between wheat and rape when determined under the same conditions of determination. The isotherm of NO3- uptake by wheat could be separated into saturated and unsaturated parts, and when the concentration of NO3- exceeded 180 uuuuuuuuuuuuM, the relationship between the rate of NO3- uptake and NO3- concentration tended to be linear. However, the isotherm of NO3- uptake by rape was found to fit the Michealis-Menten equation and no linear relationship could be found.展开更多
The adsorption kinetics of Pb^2+ on different soils and minerals with variable charges was studied by the two ion-selective electrode technique at different pH and concentrations.The results showed that more than 95% ...The adsorption kinetics of Pb^2+ on different soils and minerals with variable charges was studied by the two ion-selective electrode technique at different pH and concentrations.The results showed that more than 95% of adsorption on all the samples occurred during the first 5 minutes.All adsorption time-dependent data could fit the surface second-order equation very well.The values of Xm were goethite>kaolinite,and latosol>red soil at the same initial reaction concentration.The values of k were kaolinite>>goethite,and latosol>red soil at the same reaction pH and initial concentration.The higher the suspension pH,the faster the adsorption occurred.The meaning of Xm got by the two ion-selective electrode technique(one kind of batch technique) was different from that by the miscible displacement technique in that the former was only the equilibrium adsorption amount at a definite concentration and pH,but the latter was almost equal to the adsorption capacity at a definite pH with much less influence of concentration.One Pb^2+ was supposed to occupy two adsorption sites as the adsorption mechanism is concerned.展开更多
In the present study, the effect of one-generation divergent selection on the growth and survival of the bay scallop (Argopecten irradians concentricus) was examined to evaluate the efficacy of a selection program c...In the present study, the effect of one-generation divergent selection on the growth and survival of the bay scallop (Argopecten irradians concentricus) was examined to evaluate the efficacy of a selection program currently being carried out in Beibu Bay in the South China Sea. A total of 146 adult scallops were randomly selected from the same cultured population ofA. i. concentricus, and divided into two groups in shell length (anterior-posterior measurement): large (4.91-6.02 cm, n=74) and small (3.31-4.18 cm, n=72). At the same time, a control group was also randomly sampled (4.21-4.88 cm, n=80). Mass-spawned F1 progenies from the three size groups were obtained and reared under identical conditions at all growth phases. The effects of two-way (or upward-downward) selection on fertilization rate, hatching rate, survival rate, daily growth in shell length and body weight were assessed in the three size groups. Results show that significant differences (P〈0.01) were found in hatching rate, survival rate and daily growth of F~ progenies, but not in fertilization rate (P〉0.05), among the three groups. The hatching rate, survival rate and daily growth of the progeny of large-sized parents were greater than those of the control group (P〈0.05), which in turn were larger than those of small-sized group (P〈0.05). Responses to selection by shell length and body weight were 0.32±0.04 cm and 2.18±0.05 g, respectively, for the upward selection, and -0.14±0.03 cm and -2.77±0.06 g, respectively, for the downward selection. The realized heritability estimates of shell length and body weight were 0.38±0.06 cm and 0.22±0.07 g for the upward selection, and 0.24±0.06 cm and 0.37±0.09 g for the downward selection, respectively. The change in growth by bidirectional selection suggests that high genetic variation may be present in the cultured bay scallop population in China.展开更多
Abstract In this study, the method of divergent selection was employed to test the larval and juvenile growth performance within a full-sib family of Manila clam Ruditapes philippinarum. The 10% largest and 10% smalle...Abstract In this study, the method of divergent selection was employed to test the larval and juvenile growth performance within a full-sib family of Manila clam Ruditapes philippinarum. The 10% largest and 10% smallest clam individuals (on the basis of shell length) of a full-sib family were selected as parents for the fast and slow growing lines, respectively. The difference in shell length was significant among the three lines (fast, control, and slow) tested. The sequence of shell length were fast line 〉 control line 〉 slow line. The responses to selection, realized heritability, and genetic gain were 0.06%-0.81%, 0.04%-0.47% and 0.58%-18.89% in the fast direction, respectively; and were 0.14%-1.27%, 0.08%-0.73%, and 0.31%-49.03% in the slow direction, respectively. The re- suits suggested that there was a large portion of additive genetic variance affecting the growth in the full-sib family. Selection in the fast direction within the full-sib family would greatly improve the growth ofR. philippinarum.展开更多
文摘15-day old seedlings of wheat and rape were grown in a series of solutions with different concentrations of KNO3 for a definite period of time. The changes in NO3- concentration of the solutions were determined by the double ion-selective electrode method, and then the amount of NO3- taken up by the plants was estimated and values of Km and Imax of the Michealis-Menten equation were calculated. Results show that both the method and conditions of determination affected the values of Km and Imax. For example, the Km value was appreciably reduced when the volume of culture solution was increased or when the duration of nutrient uptake was shortened; the Km value obtained with short-term depletion method was higher than that obtained with long-term one. Similar Variations were found for the values of Imax. There was a considerable difference in the characteristics of uptake kinetics between wheat and rape when determined under the same conditions of determination. The isotherm of NO3- uptake by wheat could be separated into saturated and unsaturated parts, and when the concentration of NO3- exceeded 180 uuuuuuuuuuuuM, the relationship between the rate of NO3- uptake and NO3- concentration tended to be linear. However, the isotherm of NO3- uptake by rape was found to fit the Michealis-Menten equation and no linear relationship could be found.
基金Project supported by the National Natural Science Foundation of China.
文摘The adsorption kinetics of Pb^2+ on different soils and minerals with variable charges was studied by the two ion-selective electrode technique at different pH and concentrations.The results showed that more than 95% of adsorption on all the samples occurred during the first 5 minutes.All adsorption time-dependent data could fit the surface second-order equation very well.The values of Xm were goethite>kaolinite,and latosol>red soil at the same initial reaction concentration.The values of k were kaolinite>>goethite,and latosol>red soil at the same reaction pH and initial concentration.The higher the suspension pH,the faster the adsorption occurred.The meaning of Xm got by the two ion-selective electrode technique(one kind of batch technique) was different from that by the miscible displacement technique in that the former was only the equilibrium adsorption amount at a definite concentration and pH,but the latter was almost equal to the adsorption capacity at a definite pH with much less influence of concentration.One Pb^2+ was supposed to occupy two adsorption sites as the adsorption mechanism is concerned.
基金Supported by the National Science and Technology Ministry(No.2007GB2E000183)the Agricultural Ministry of China(No.nyhyzx07-047)
文摘In the present study, the effect of one-generation divergent selection on the growth and survival of the bay scallop (Argopecten irradians concentricus) was examined to evaluate the efficacy of a selection program currently being carried out in Beibu Bay in the South China Sea. A total of 146 adult scallops were randomly selected from the same cultured population ofA. i. concentricus, and divided into two groups in shell length (anterior-posterior measurement): large (4.91-6.02 cm, n=74) and small (3.31-4.18 cm, n=72). At the same time, a control group was also randomly sampled (4.21-4.88 cm, n=80). Mass-spawned F1 progenies from the three size groups were obtained and reared under identical conditions at all growth phases. The effects of two-way (or upward-downward) selection on fertilization rate, hatching rate, survival rate, daily growth in shell length and body weight were assessed in the three size groups. Results show that significant differences (P〈0.01) were found in hatching rate, survival rate and daily growth of F~ progenies, but not in fertilization rate (P〉0.05), among the three groups. The hatching rate, survival rate and daily growth of the progeny of large-sized parents were greater than those of the control group (P〈0.05), which in turn were larger than those of small-sized group (P〈0.05). Responses to selection by shell length and body weight were 0.32±0.04 cm and 2.18±0.05 g, respectively, for the upward selection, and -0.14±0.03 cm and -2.77±0.06 g, respectively, for the downward selection. The realized heritability estimates of shell length and body weight were 0.38±0.06 cm and 0.22±0.07 g for the upward selection, and 0.24±0.06 cm and 0.37±0.09 g for the downward selection, respectively. The change in growth by bidirectional selection suggests that high genetic variation may be present in the cultured bay scallop population in China.
基金supported by the grant from the Natural National Science Foundation of China (No.31502163)the fund earmarked for Modern Agro-industry Technology Research System (CARS-48)the Natural Science Foundation Grant of Tianjin (No.15JCYBJC30400)
文摘Abstract In this study, the method of divergent selection was employed to test the larval and juvenile growth performance within a full-sib family of Manila clam Ruditapes philippinarum. The 10% largest and 10% smallest clam individuals (on the basis of shell length) of a full-sib family were selected as parents for the fast and slow growing lines, respectively. The difference in shell length was significant among the three lines (fast, control, and slow) tested. The sequence of shell length were fast line 〉 control line 〉 slow line. The responses to selection, realized heritability, and genetic gain were 0.06%-0.81%, 0.04%-0.47% and 0.58%-18.89% in the fast direction, respectively; and were 0.14%-1.27%, 0.08%-0.73%, and 0.31%-49.03% in the slow direction, respectively. The re- suits suggested that there was a large portion of additive genetic variance affecting the growth in the full-sib family. Selection in the fast direction within the full-sib family would greatly improve the growth ofR. philippinarum.