[Objective] The study was to explore whether antimicrobial activity of the antimicrobial peptides extracted from immunized Tenebrio molitor varied with different pathogens as inducers.[Method]By inducing T.molitor hun...[Objective] The study was to explore whether antimicrobial activity of the antimicrobial peptides extracted from immunized Tenebrio molitor varied with different pathogens as inducers.[Method]By inducing T.molitor hungry larvaes to generate immune response via feeding with bacteria and with fungi or actinomycete post to pricking,the antimicrobial peptides extracts were obtained by grinding and centrifuging the cultures.Its antimicrobial activity against 26 pathogens was measured by bacteriostatic ring,and evaluated by trisection to four types and inhibitory spectrum.[Result]Both the antimicrobial activity and antimicrobial spectrum of the antimicrobial peptides extracts varied remarkably among different pathogens as inducers.[Conclusion]Bio-control used strains have obvious advantage in inducing the insect to express body fluid immunity material-antimicrobial peptides.展开更多
The travel time and amplitude of ground-penetrating radar （GPR） waves are closely related to medium parameters such as water content, porosity, and dielectric permittivity. However, conventional estimation methods, ...The travel time and amplitude of ground-penetrating radar （GPR） waves are closely related to medium parameters such as water content, porosity, and dielectric permittivity. However, conventional estimation methods, which are mostly based on wave velocity, are not suitable for real complex media because of limited resolution. Impedance inversion uses the reflection coefficient of radar waves to directly calculate GPR impedance and other parameters of subsurface media. We construct a 3D multiscale stochastic medium model and use the mixed Gaussian and exponential autocorrelation function to describe the distribution of parameters in real subsurface media. We introduce an elliptical Gaussian function to describe local random anomalies. The tapering function is also introduced to reduce calculation errors caused by the numerical simulation of discrete grids. We derive the impedance inversion workflow and test the calculation precision in complex media. Finally, we use impedance inversion to process GPR field data in a polluted site in Mongolia. The inversion results were constrained using borehole data and validated by resistivity data.展开更多
In order to delay or eliminate the occurrence and expansion of the reflective cracking in the asphalt concrete overlay on old cement concrete pavement, an epoxy asphalt geogrid stress-absorbing layer（ EAGSAL） was de...In order to delay or eliminate the occurrence and expansion of the reflective cracking in the asphalt concrete overlay on old cement concrete pavement, an epoxy asphalt geogrid stress-absorbing layer（ EAGSAL） was designed. The EAGSAL consists of epoxy asphalt and fiberglass geogrid. The pull-out test, skewshearing test, bending beam test and fatigue test were conducted to evaluate the performance of the EAGSAL and a traditional stress-absorbing layer（ TSAL）. The results showthat the adhesive performance, shear performance, bending strength and fatigue performance of the EAGSAL with an optimal spraying volume of epoxy asphalt are better than those of optimally designed TSAL, and the maximum bending strain of the EAGSAL is very close to that of the TSAL. The EAGSAL has superior performance in reflective cracking resistance.Moreover, the EAGSAL with the optimal spraying volume of approximately 2. 0 L m^2 is thinner and lighter than the TSAL,which can decrease the thickness and improve the bearing ability of the whole pavement structure.展开更多
When oleanolic acid (OA) was administered ig before and after sensitization on d 1 to d 5 and d 11 to d 17,it had no apparent effect on Arthus reaction.When it was administered at 48,24 and 1 h before challenge,howeve...When oleanolic acid (OA) was administered ig before and after sensitization on d 1 to d 5 and d 11 to d 17,it had no apparent effect on Arthus reaction.When it was administered at 48,24 and 1 h before challenge,however,Arthus reaction was significantly inhibited.OA showed markedly suppressive effects on reversible passive Arthus reaction and leukocyte migratory response.It could significantly stabilize erythrocyte membrane,inhibit the swelling of the rat's hind paw induced by in- jecting mycostatin,reduce the acid phosphatase content in the inflammatory exudate,suppress the syn- thesis or release of PGE,histamine,LTB4 and kinin,and the phlogistic action of PGE_2,histamine,5- HT and kinin.In addition,it could decrease the content of malonyldialdehyde (MDA) in hepatic tissue of alcohol-intoxicated mice,and increase the activity of catalase (CAT) in hepatic tissue of mice.OA had no apparent effect on the activity of super-oxide dismutase (SOD) in rat serum,on the content of immune complex in serum of rat with Arthus reaction,on the phagocytosis of monocytc-macrophage system,on the clearance of enzyme-containing immune complex by macrophage,or on the activity of total complement.展开更多
基金Supported by Natural Science Foundation of Hebei Province(C200700450)~~
文摘[Objective] The study was to explore whether antimicrobial activity of the antimicrobial peptides extracted from immunized Tenebrio molitor varied with different pathogens as inducers.[Method]By inducing T.molitor hungry larvaes to generate immune response via feeding with bacteria and with fungi or actinomycete post to pricking,the antimicrobial peptides extracts were obtained by grinding and centrifuging the cultures.Its antimicrobial activity against 26 pathogens was measured by bacteriostatic ring,and evaluated by trisection to four types and inhibitory spectrum.[Result]Both the antimicrobial activity and antimicrobial spectrum of the antimicrobial peptides extracts varied remarkably among different pathogens as inducers.[Conclusion]Bio-control used strains have obvious advantage in inducing the insect to express body fluid immunity material-antimicrobial peptides.
基金supported by the Doctoral Fund Project of the Ministry of Education(No.20130061110060 class tutors)the Post-Doctoral Fund Project(No.2015M571366)+1 种基金the National Natural Science Foundation of China(No.41174097)US DoD ARO Project"Advanced Mathematical Algorithm"(No.W911NF-11-2-0046)
文摘The travel time and amplitude of ground-penetrating radar （GPR） waves are closely related to medium parameters such as water content, porosity, and dielectric permittivity. However, conventional estimation methods, which are mostly based on wave velocity, are not suitable for real complex media because of limited resolution. Impedance inversion uses the reflection coefficient of radar waves to directly calculate GPR impedance and other parameters of subsurface media. We construct a 3D multiscale stochastic medium model and use the mixed Gaussian and exponential autocorrelation function to describe the distribution of parameters in real subsurface media. We introduce an elliptical Gaussian function to describe local random anomalies. The tapering function is also introduced to reduce calculation errors caused by the numerical simulation of discrete grids. We derive the impedance inversion workflow and test the calculation precision in complex media. Finally, we use impedance inversion to process GPR field data in a polluted site in Mongolia. The inversion results were constrained using borehole data and validated by resistivity data.
基金The National Natural Science Foundation of China(No.51178114,51378122)
文摘In order to delay or eliminate the occurrence and expansion of the reflective cracking in the asphalt concrete overlay on old cement concrete pavement, an epoxy asphalt geogrid stress-absorbing layer（ EAGSAL） was designed. The EAGSAL consists of epoxy asphalt and fiberglass geogrid. The pull-out test, skewshearing test, bending beam test and fatigue test were conducted to evaluate the performance of the EAGSAL and a traditional stress-absorbing layer（ TSAL）. The results showthat the adhesive performance, shear performance, bending strength and fatigue performance of the EAGSAL with an optimal spraying volume of epoxy asphalt are better than those of optimally designed TSAL, and the maximum bending strain of the EAGSAL is very close to that of the TSAL. The EAGSAL has superior performance in reflective cracking resistance.Moreover, the EAGSAL with the optimal spraying volume of approximately 2. 0 L m^2 is thinner and lighter than the TSAL,which can decrease the thickness and improve the bearing ability of the whole pavement structure.
文摘When oleanolic acid (OA) was administered ig before and after sensitization on d 1 to d 5 and d 11 to d 17,it had no apparent effect on Arthus reaction.When it was administered at 48,24 and 1 h before challenge,however,Arthus reaction was significantly inhibited.OA showed markedly suppressive effects on reversible passive Arthus reaction and leukocyte migratory response.It could significantly stabilize erythrocyte membrane,inhibit the swelling of the rat's hind paw induced by in- jecting mycostatin,reduce the acid phosphatase content in the inflammatory exudate,suppress the syn- thesis or release of PGE,histamine,LTB4 and kinin,and the phlogistic action of PGE_2,histamine,5- HT and kinin.In addition,it could decrease the content of malonyldialdehyde (MDA) in hepatic tissue of alcohol-intoxicated mice,and increase the activity of catalase (CAT) in hepatic tissue of mice.OA had no apparent effect on the activity of super-oxide dismutase (SOD) in rat serum,on the content of immune complex in serum of rat with Arthus reaction,on the phagocytosis of monocytc-macrophage system,on the clearance of enzyme-containing immune complex by macrophage,or on the activity of total complement.
