食物不耐受特异性IgG抗体与肠易激综合征关系的临床研究

目的探讨食物不耐受特异性Ig G抗体与肠易激综合征(IBS)的关系。方法对128例IBS患者和80例健康体检者,应用酶联免疫吸附法半定量检测血清中14种食物不耐受特异性lg G抗体水平并分为0、+1、+2和+3级;根据检测结果剔除相应食物治疗8周,采用调查表评分系统对IBS患者的症状进行评分以评价疗效。结果 128例IBS患者中,至少有1种食物不耐受特异性Ig G抗体阳性112例,阳性率为87.5%,明显高于对照组的9.3%(P<0.05)。IBS患者食物不耐受特异性Ig G阳性率以蟹(84.4%)和虾(75.0%)较高。128例IBS患者中Ig G抗体阳性+2以上的患者82例,经剔除相应食物治疗8周后,临床症状明显改善56例(68.3%),症状有所改善16例(19.5%),无效10例(12.2%),总有效率为87.8%(72/82)。结论根据IBS患者血清中食物不耐受特异性Ig G水平,剔除相应食物,能有效地改善IBS的临床症状。

治疗性沟通对老年脑卒中病人自我感受负担的影响

[目的]探讨治疗性沟通对老年脑卒中病人自我感受负担(SPB)的影响。[方法]采用随机数字表法将60例存在SPB的老年脑卒中病人分夫妇为为两组各30例,对照组给予常规护理及一般性沟通,干预组在此基础上给予治疗性沟通,采用自我感受负担量表(SPBS)评价干预效果。[结果]干预后两组病人SPB总分、照护负担维度、情感负担维度得分以及SPB程度比较差异有统计学意义(P<0.01或P<0.05)。[结论]治疗性沟通能有效减轻老年脑卒中病人的自我感受负担。

试说《儒林外史》为“儒林”、“写实”小说——兼及鲁迅“讽刺之书”说的思考

《儒林外史》在题材上是一部写明代以及清代的"儒林小说",它源于史家"实录"精神的高度"写实"的文学成就,虽使其有"足称讽刺之书"之誉,但"讽刺"仅其"写实"效果的一面,又系读者"受性"自得,而非作者有意为之。《儒林外史》的总体艺术风格是"写实",而非"讽刺"。《儒林外史》是我国古代最优秀、最纯粹的"儒林"题材"写实"风格的长篇小说。

Experimental and numerical study on flexural behaviors of steel reinforced engineered cementitious composite beams

To investigate the flexural behaviors of steel reinforced engineered cementitious composite （ECC） beams, the behaviors of the steel reinforced ECC beam and the conventional steel reinforced concrete beam subjected to flexural load are experimentally compared. The experimental results show that the flexural strength and ductility of the steel reinforced ECC beam are 24.8% and 187.67% times larger than those of the steel reinforced concrete beam, and the substitution of concrete with ECC can significantly delay the propagation of cracks. Additionally, a simplified constitutive model of the ECC material is used to simulate the flexural behaviors of beams by the finite element analysis （FEA）. The results show a good agreement between the simulation and test results. The crack width of the steel reinforced ECC beam can be limited to 0.4 mm under the service load conditions. The application of ductile ECC can significantly increase the flexural performance in terms of flexural strength, deformation capacity and ductility of the beams.

Experimental study on the effects of climatic characteristics on people's adaptability to thermal environment

In order to find out how the climatic characteristics affect people＇s adaptability to thermal environments,experimental studies in a climate chamber are conducted on the effects of transition seasons（from spring to summer）and the occupants＇ native areas on indoor thermal sensations.Results reveal that people＇s tolerances to cool and warm indoor environments are different in the transition season.When the outdoor temperature is higher,the occupants have a weaker tolerance to a cool indoor environment,but a stronger tolerance to a warm indoor environment.Besides,it is found that the occupants＇ thermal sensations depend on both the climatic characteristics of the season and their native areas.The people from southern China present a greater tolerance to both warm and cool indoor environments than those from northern China.The reason can be explained according to the occupants＇ adaptability to the climatic characteristics and the indoor thermal environments of their native areas in different climate zones.

Isolation of Ca^(2+) Tolerant Cardiomyocytes from Aadult Rats for Patch Clamp Studies

Objective: To investigate the factors affecting the viability and Ca 2+ tolerance of isolated rats' cardiac myocytes for patch clamp research. Methods: Hearts were firstly perfused by the Langendorff perfusion apparatus with normal Tyrode's solution, then with Ca 2+-free Tyrode's solution and subsequently with low Ca 2+ enzyme solution containing collagenase 0.1-0.2 g/L. All the solutions were saturated with oxygen and the perfusion temperature was kept at 37 ℃. Finally hearts were washed by Ca 2+-free Tyrode's solution, after which the ventricles were minced into small pieces in KB solution, dispersed and filtered. The isolated myocytes were stored in KB solution at room temperature for 1 h and recovered to normal calcium concentration before patch clamp experiments.Results: When all the factors such as water, enzyme, Ca 2+,pH, and oxygen were well controlled, the well constructed and rod-like cardiac myocytes with a yielding rate of 30%-50% came out.Conclusion: All the factors should be well controlled, which ensured the isolated cells Ca 2+ tolerant and appropriate for patch clamp experiments.

Mechanical properties of monodirectional Gutou mortise-tenon joints of the traditional timber buildings in the Yangtze River region

The mechanical properties of the monodirectional Gutou mortise-tenon joints of the ancient Chinese traditional timber buildings in the Yangtze River region were studied using the experimental method. Three monodirectional Gutou mortise-tenon joints were designed according to the actual configurations. The failure modes,the hysteretic curves,the skeleton curves, the rotation rigidities, and the energy dissipation capacity of this type of mortise-tenon joints under the low cyclic reversed loading were obtained. The results show that the hysteretic curves of the monodirectional Gutou mortise-tenon joints appear to be Z shape and have obvious pinch effects. During the process of the test,these mortisetenon joints pass orderly through the elastic stage,the yield stage and the failure stage. The energy dissipation capacity of these mortise-tenon joints generally decreases with the increase in the rotation angle. The equivalent viscous damping coefficients of the monodirectional Gutou mortise-tenon joints are between 0. 161 and 0. 193. The results can provide the theoretical base for the computing analysis and repair design of Chinese traditional timber buildings in the Yangtze River region.

Flexural behaviors of FRP strengthened corroded RC beams

The flexural behavior of eight FRP （ fiber reinforced polymer） strengthened RC （reinforced concrete） beams with different steel corrosion rates are numerically studied by Ansys finite element software. The influences of the corrosion rate on crack pattern, failure mechanism, ultimate strength, ductility and deformation capacity are also analyzed. Modeling results show that the beams with low corrosion rates fail by the crushing of the concrete in the compression zone. For the beams with medium corrosion rates, the bond slip between the concrete and the longitudinal reinforcement occurs after steel yielding, and the beams finally fail by the debonding of the FRP plates. For the beams with high corrosion rates, the bond slip occurs before steel yielding, and the beams finally fail by the crushing of the concrete in the compression zone. The higher the corrosion rates of the longitudinal reinforcement, the more the carrying capacity of FRP strengthened RC beams reduces. The carrying capacity of RCB-1 （the corrosion rate is 0） is 115 kN, and the carrying capacity of RCB-7 （the corrosion rate is 20% ） is 42 kN. The deformation capacity of FRP strengthened corroded RC beams is higher than that of FRP strengthened uucorroded RC beams. The ultimate deflection of RCB-1 and RCB-7 are 20 mm and 35 nun, respectively, and the ultimate deflection of RCB-5 （the corrosion rate is 10% ） reaches 60 ilUn.

Safety and Tolerance of Adefovir Dipivoxil in Chinese Healthy Volunteers: A PhaseⅠRandomized and Open-Label Trial

Aim To assess the safety and tolerance of adefovir dipivoxil （ADV） in Chinese healthy volunteers. Methods A total of 52 healthy volunteers, 26 males and 26 females, aged from 19 to 26 were enrolled in the study. Forty-two subjects were randomized into 5, 10, 20, 40, and 60 mg dose groups （6 - 10 subjects in each） matched by sex and weight for single-dose trial. Ten subjects were orally given 10 mg of ADV tablets once daily for 7 d for multiple-dose trial. Physical examination, vital signs examination, electrocardiography, type-B ultrasonography, chest fluoroscopy, routine blood test, routine urine test, coagulation tests, and blood biochemical test were conducted on schedule and statistically evaluated. Results Asthenia frequently occurred in multiple-dose trial, nausea, abdominal pain, and diarrhea occurred in both single- and multiple-dose trials. ALT, bilirubin, CK, and LDH were slightly elevated. All adverse reactions and laboratory abnormalities were mild, and the frequency and severity were not related to doses. Conclusion ADV is safe and well tolerated in Chinese healthy volunteers at dose of 5 - 60 mg oncedaily or 10 nag once daily for 7 d. The recommended oral dosage regimen is 10 mg once daily. Attention should be paid to renal and liver functions, CK, AMY and LDH, if we take ADV for a long period of time.

Effects of Salinity on Growth of Tilapia nilotica and Tilapia mossambica

Tilapia has wide-range tolerance to salinity, and most Tilapia species can survive in both fresh water and seawater. In this study, the survival rates, absolute growth rates and instantaneous growth rates of Tilapia nilotica and Tilapia mossambica in waters with four different salinities（0‰, 10‰, 20‰, 30‰） were investigated.The results showed that the growth of T. mossambica was trended to be increased with the increased salinity, and it is suitable to be farmed in seawater; however, the growth of T. nilotica was trended to be decreased with the increased salinity, and it is suitable to be farmed in fresh water.

Is the Potential Interconnection between Antibiotic Susceptibility and Disinfectant Resistance a Threat to Food Safety?

The prevalence of antibiotic susceptibility and disinfectant resistance in bacterial pathogens causes a serious problem not only to food safety but also to public health, which directly or indirectly leads to treatment and disinfection failures. In this review, multidrug resistance, the mechanism of disinfectant resistance, the methods for detecting disinfectant resistance and the cross-resistance between an- tibiotics and disinfectants are summarized. More efforts should be devoted to explor- ing the professional guidance of using antibiotics and disinfectants, and to develop- ing the comprehensive detection with genetic and molecular methods is highly ex- pected.

Comparison on the Tolerance of Cruciferous Crops and Leguminous Crops to Microcystin

[Objective] To study the differences in the tolerance of leguminous crops and cruciferous crops to microcystin （MC）. [Methed] The cruciferous typical crops oilseed rapes, pakchois, cabbages and leguminous typical crops soybeans, peas and broad beans were selected as the materials to test the effects of MC of differ- ent concentrations on the germination, growth and development of leguminous crops and cruciferous crops. The measurement indicators included germination rate, plant height, chlorophyll, etc. [Result] The MC had great effects on the oilseed rape and pakchois of cruciferous crops, and smaller effects on cabbage, while the leguminous crops were generally not affected. [Conclusion] Leguminous crops are more tolerant to MC than cruciferous crops and more preferential in MC polluted regions.

Experimental Study on Force Behavior of Steel Rein forced Concrete Transfer Beam Structure with Basement of Large Space

Based on comparative test of two transfer story models, in one of which the transfer beam and basement column is constructed of steel reinforced concrete, and the other is constructed of ordinary reinforced concrete, its force behavior, ductility and failure mechanism under vertical and horizontal loads are studied. The results show that loading bearing and seismic behavior of transfer story structure with steel reinforced concrete beam and basement column is good. The relative design suggestion is put forward.

RESEARCH ON T SHAPED HIGH STRENGTH CONCRETE MEMBERS SUBJECTED TO AXIAL COMPRESSION AND BIAXIAL BENDING

Based on experiments, a computer program is developed. The calculated results agree well with the experimental results. The flexural behavior of T shaped high strength concrete members subjected to axial compression and biaxial bending is studied. The main factors affecting the flexural behavior of T shaped high strength concrete members are loading angle, axial compression ratio and reinforcement ratio.

Analysis on Polymorphisms of GHR Gene in Improved Hybrid Yellow Cattle Groups from Liupan Mountain Area in Ningxia

[Objective] The paper aimed to analyze the polymorphism of the growth hormone receptor （GHR） in improved hybrid yellow cattle group from Liupan Mountain area in Ningxia Autonomous Region,so as to provide technological basis for hybrid improvement. [Method] Polymerase chain reaction-restriction fragment length polymorphisms （PCR-RFLP） technology was carried out to examine polymorphisms of GHR gene of 70 individuals. [Result] The target fragment of 338 bp was amplified. The PCR product digested by restriction enzyme Alu I showed polymorphisms. The frequencies of the two genotypes （AA,BB） were 75.71% （53 individuals） and 24.29% （17 individuals）,respectively. [Conclusion] Two genotypes of GHR gene were detected in improved hybrid yellow cattle groups from Liupan Mountain area in Ningxia.

Study on the Developmental Changes of Muscular GHR mRNA Expression in Sheep

[ Objective] The study aimed to explore the expression of muscular growth hormone receptor gene （GHR） in sheep at the early stage of growth and development. [Method] The GHR mRNA expression levels in longissimus dorsal muscles of male Kazak sheep and Xinjiang fine wool sheep with different ages were quantitatively analyzed by real time PCR. [ Result] Sheep GHR mRNA expression level in longissimus dorsal muscle increased firstly followed by decline, and then kept steady until the end of the experiment, with the expression peak appearing on postnatal day 30. The GHR mRNA expression level of Kazak sheep was extremely lower than that of Xingjiang fine wool sheep from 2 to 90 days old （ P 〈0.01 ）. E Conclusionl Both age and breed had great effects on the expression of muscular GHR gene in sheep.

Design and Realization of Cold Disaster Early Warning System for Subtropics Aquaculture

By analyzing the subtropics aquaculture present situation,the necessity of the construction of cold disaster early warning system for subtropics aquaculture,the research goal and the duty were expounded. The system structure and the frame were introduced in detail. Several key questions and their solutions of the cold disaster early warning system for subtropics aquaculture were put forward.

Experiment and simulation of creep performance of basalt fibre asphalt mortar under uniaxial compressive loadings

The creep performance of basalt fibre（BF）reinforced in asphalt mortar under uniaxial compressive loadings is investigated. The samples of basalt fibre asphalt mortar（BFAM） with different BF mass fractions（0. 1%,0. 2%, and 0. 5%） and without BF in asphalt mixture are prepared, and then submitted for the compressive strength test and corresponding creep test at a high in-service temperature.Besides, numerical simulations in finite element ABAQUS software were conducted to model the compressive creep test of mortar materials, where the internal structure of the fibre mortar was assumed to be a two-component composite material model such as fibre and mortar matrix. Finally, the influence factors of rheological behaviors of BFAM are further analyzed. Results indicate that compared to the control sample, the compressive strength of BFAM samples has a significant increase, and the creep and residual deformation are decreased. However, it also shows that the excessive fibre, i.e. with the BF content of 0. 5%, is unfavorable to the high-temperature stability of the mortar. Based on the analysis results, the prediction equations of parameters of the Burgers constitutive model for BFAM are proposed by considering the fibre factors.

Strength of circular concrete columns under concentric compression

An experimental study, in which six columns were loaded concentrically toinvestigate the behavior of reinforced normal strength and high strength circular columns underconcentric compression, is described. The concrete strengths of the columns were 30 MPa and 60 MPa.The primary variables considered were the concrete strength and the amount of transversereinforcement. Test results indicate that smaller hoop spacing provides higher column capacity andgreater strength enhancement in a confined concrete core of columns. For the same lateralconfinement, high strength concrete columns develop lower strength enhancement than normal strengthconcrete columns. Both the strength enhancement ratio (f'_(cc) /f'_(co)) and the column capacityratio (P_(test)/P_o) were observed to show linear increase variations with rho_s f_(yt)/f'_c incircular columns.

Reliability analysis of piles constructed on slopes under laterally loading

Response surface method is used to study the reliability analysis of laterally loaded piles in sloping ground. A development load-displacement （p-y） curve for laterally loaded pile response in sloping ground is used to model the pile-soil system, both the pile head displacement and the maximum bending moment of the piles are used as the performance criteria in this study. The reliability analysis method of the laterally loaded pile in sloping ground under the pile head displacement and the maximum bending moment failure modes is proposed, which is in good agreement with the Monte Carlo method. The influences on the probability index of failure by a number of parameters are discussed. It is shown that the variability of pile head displacement increases with the increase in the coefficients of variation of ultimate bearing capacity factor （Npu）, secant elastic modulus at 50%（E50） and level load （H）. A negative correlation between Npu and non-dimensional factor （λ） leads to less spread out probability density function （PDF） of the pile head displacement;in contrast, a positive correlation between Npu andλgives a great variation in the PDF of pile head displacement. As for bearing capacity factor on ground surface （Npo） and λ, both negative and positive correlations between them give a great variation in the PDF of pile head displacement, and a negative correlation will obviously increase the variability of the response.