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住宅建筑设计的“可变性”
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作者 王兆勋 金凤 《数码设计》 2020年第13期104-105,共2页
住宅建筑在建设部制定的《商品住宅装修一次到位实施导则》中要求在交付时,所有功能空间的固定面全部铺装或粉刷完成,厨房和卫生间的基本设备全部安装完成;这样使得根据使用需求改造难度增加,文本提出“可变住宅设计”的理念,结合平面... 住宅建筑在建设部制定的《商品住宅装修一次到位实施导则》中要求在交付时,所有功能空间的固定面全部铺装或粉刷完成,厨房和卫生间的基本设备全部安装完成;这样使得根据使用需求改造难度增加,文本提出“可变住宅设计”的理念,结合平面功能布局和构造方式,实现使用功能及方式的转换住宅空间形成“可变空间”。 展开更多
关键词 “可变性” 住宅设计 功能布局
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Graft-induced Inheritable Variation in Mungbean and Its Application in Mungbean Breeding 被引量:22
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作者 张丹华 孟昭璜 +2 位作者 肖卫民 王学臣 苏都莫日根 《Acta Botanica Sinica》 CSCD 2002年第7期832-837,共6页
Graft can induce inheritable variations in the progenies of the scion plants. Seedling of mungbean ( Vigna radiata (L.) Wilczek) was grafted onto the stem of sweet potato ( Ipomoea batatas (L.) Lam.). The growth... Graft can induce inheritable variations in the progenies of the scion plants. Seedling of mungbean ( Vigna radiata (L.) Wilczek) was grafted onto the stem of sweet potato ( Ipomoea batatas (L.) Lam.). The growth of the scion was maintained until the scion produced selfed seeds. We sowed the seeds for several generations under normal conditions. Distinct genetic variations appeared in the progenies. Similar variations did not appear in the generations of the scion sowed normally without graft. The variations seemed to be induced by the graft and they inherited steadily. For understanding the possible mechanism of the phenomenon (graft_induced inheritable variation), we analyzed the cytoplasmic and genomic DNA of the variations. The results showed that there was no restriction fragment length polymorphism (RFLP) in the cytoplasmic DNA between the original scion and the variation. However, significant difference between the scion and variation was recognized by random amplified polymorphic DNA (RAPD) analysis. In addition, there was no evidence that indicated the gene transformation from stock to scion. Our results suggest that the non_specific grafting has a pragmatic potential for plant breeding and crop improvement and, the genetic variation seems not to be caused simply by DNA transformation but most likely the stress induced mutation. 展开更多
关键词 GRAFT genetic variation MUNGBEAN sweet potato RAPD analysis crop improvement
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Multiple attribute decision making method based on trapezoid fuzzy linguistic variables 被引量:4
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作者 梁雪春 陈森发 《Journal of Southeast University(English Edition)》 EI CAS 2008年第4期478-481,共4页
The problem of multiple attribute decision making under fuzzy linguistic environments, in which decision makers can only provide their preferences (attribute values)in the form of trapezoid fuzzy linguistic variable... The problem of multiple attribute decision making under fuzzy linguistic environments, in which decision makers can only provide their preferences (attribute values)in the form of trapezoid fuzzy linguistic variables(TFLV), is studied. The formula of the degree of possibility between two TFLVs is defined, and some of its characteristics are studied. Based on the degree of possibility of fuzzy linguistic variables, an approach to ranking the decision alternatives in multiple attribute decision making with TFLV is developed. The trapezoid fuzzy linguistic weighted averaging (TFLWA) operator method is utilized to aggregate the decision information, and then all the alternatives are ranked by comparing the degree of possibility of TFLV. The method can carry out linguistic computation processes easily without loss of linguistic information, and thus makes the decision results reasonable and effective. Finally, the implementation process of the proposed method is illustrated and analyzed by a practical example. 展开更多
关键词 trapezoid fuzzy linguistic variables degree of possibility multiple attribute decision making
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Development of Turbocharger for Improving Diesel Engine Matching Performance 被引量:4
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作者 张虹 马朝臣 《Journal of Beijing Institute of Technology》 EI CAS 2002年第2期146-149,共4页
The two development ways of turbocharger technology to solve the problem of matching performance with diesel were presented. The ways of waste valve gate turbocharger and variable geometry turbocharger can solve the p... The two development ways of turbocharger technology to solve the problem of matching performance with diesel were presented. The ways of waste valve gate turbocharger and variable geometry turbocharger can solve the problem of engine’s low speed torque and achieve lower smoke level. Especially for variable geometry turbocharger, it covers all conditions of engine. It can not only improve the low engine’s speed torque and keep the power performance at high engine speed, but also cover wide engine speed performance that keeps lower fuel consumption and exhaust gas temperature in full load and part load matching. The results of theory analysis and experiment research showed that it’s the ideal solution to solve the matching problem of diesel engines. 展开更多
关键词 TURBOCHARGER match performance variable geometry turbocharger
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Single-Stage Wide-Range CMOS VGA with Temperature Compensation and Linear-in-dB Gain Control 被引量:1
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作者 恽廷华 尹莉 +1 位作者 吴建辉 时龙兴 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2007年第4期518-525,共8页
A novel wide-range CMOS variable gain amplifier (VGA) topology is presented. The proposed VGA is composed of a variable transconductor and a novel variable output resistor and can offer a high gain variation range o... A novel wide-range CMOS variable gain amplifier (VGA) topology is presented. The proposed VGA is composed of a variable transconductor and a novel variable output resistor and can offer a high gain variation range of 80dB while using a single variable-gain stage. Temperature-compensation and decibel-linear gain characteristic are achieved by using a control circuit that provides a gain error lower than ±1.5dB over the full temperature and gain ranges. Realized in 0.25μm CMOS technology, a prototype of the proposed VGA provides a total gain range of 64.5dB with 55.6dB-linear range,a P-1dB varying from - 17.5 to 11.5dBm,and a 3dB-bandwith varying from 65 to 860MHz while dissipating 16.5mW from a 2.5V supply voltage. 展开更多
关键词 linear-in-dB temperature compensation variable-gain amplifier automatic gain control
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Reliability supply chain network design model for perishable products 被引量:1
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作者 于海生 赵林度 《Journal of Southeast University(English Edition)》 EI CAS 2007年第S1期94-98,共5页
The classical supply chain network(SCN)design problem is extended,where the candidate facilities are subject to failure and the products are prone to elapsed time deteriorion.First,the reliable SCN design problem is d... The classical supply chain network(SCN)design problem is extended,where the candidate facilities are subject to failure and the products are prone to elapsed time deteriorion.First,the reliable SCN design problem is defined by introducing the probability that a facility may be prone to inactivity based on the analysis of perishable product characteristics.The perishable product SCN design problem is formulated as a 0-1 integer programming model.The objective is to minimize the weighted sum of the operating cost(the fixed plus transportation cost)and the expected failure cost.And then,the perishable product SCN design model is discussed and solved using the genetic algorithm(GA).The results show how to generate the tradeoff curve between the operating costs and the expected failure costs.And these tradeoff curves demonstrate empirically that substantial improvements in reliability are often possible with minimal increase in the operating costs. 展开更多
关键词 supply chain network perishable product network design RELIABILITY
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Physical-chemical and Agronomical Analysis of a Leaf Color Mutant of Rice
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作者 王丽 赵德明 +2 位作者 李云武 杨丛金 王峰 《Agricultural Science & Technology》 CAS 2010年第9期168-170,共3页
[Objective] This study was to investigate the physical-chemical properties and the agronomical traits of a leaf color mutant K7231,so as to provide scientific basis for its use as breeding material.[Method]Before head... [Objective] This study was to investigate the physical-chemical properties and the agronomical traits of a leaf color mutant K7231,so as to provide scientific basis for its use as breeding material.[Method]Before heading,the contents of chlorophyll and soluble sugar of leaf color mutant K7231 were measured,and its agronomic traits after mature were investigated.[Result]The contents of chlorophyll and soluble sugar of leaf color mutant K7231 before heading assumed a regular change law.Except the lower yield,K7231 performed similar agronomic traits compared with the CK.[Conclusion]This study provided scientific basis for the utilization of this mutant. 展开更多
关键词 MUTANT CHLOROPHYLL Soluble sugar Agronomic traits
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Temporal-Spatial Variability of Soil Organic Carbon Stocks in a Rehabilitating Ecosystem 被引量:43
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作者 ZHANGShi-Rong SUNBo +2 位作者 ZHAOQi-Guo XIAOPeng-Fei SHUJian-Ying 《Pedosphere》 SCIE CAS CSCD 2004年第4期501-508,共8页
In global change research, changes of soil organic carbon (SOC) reservoirs intropical and subtropical regions are still unknown. The temporal-spatial variability of SOC stockswas determined in a basin of over 579 km^2... In global change research, changes of soil organic carbon (SOC) reservoirs intropical and subtropical regions are still unknown. The temporal-spatial variability of SOC stockswas determined in a basin of over 579 km^2 in subtropical China from 1981to 2002. ArcGIS8.l softwarewas utilized for spatial analysis of semivariance, ordinary kriging (OK), and probability kriging(PK). Grid and hierarchical approaches were employed for the sampling scenario in 2002 with 106Global Position System (GPS) established spots sampled. Bulk topsoil samples (0—30 cm) werecollected at three random sites on each spot. The SOC content for 1981 came from the SOC map of theSecond National Soil Survey. Geostatistical results of the nugget to sill ratio (0.215-0.640)in therehabilitating ecosystem indicated a moderate spatial dependence for SOC on this large scale. Therange of SOC changed from 2.04 km in 1981 to 7.15 km in 2002. The mean topsoil SOC increased by 4.6%from 10.63 g kg^(-1) (1981) to 11.12 g kg^(-1)(2002). However, during this 21-year period 25.2% ofthe total basin area experienced a decrease in SOC. Also, the probability kriging results showedthat the geometric mean probabilities of SOC <= 6.0 g kg^(-1), <= 11.0 g kg^(-1) and > 15.0 gkg^(-1) were 0.188, 0.534 and 0.378, respectively in 2002, comparing to 0.234, 0.416 and 0.234 inthat order in 1981, respectively. The SOC storage in the topsoil increased by 17.0% during this timewith the main increase occurring in forests and cultivated land,which amounted to 82.5% and 17.0%of the total increase, respectively. 展开更多
关键词 ordinary kriging probability kriging rehabilitating ecosystem soil organiccarbon temporal-spatial variability
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The variability of eddy ki/netic energy in the South China Sea deduced from satellite altimeter data 被引量:13
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作者 陈更新 侯一筠 +2 位作者 储小青 齐鹏 胡珀 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2009年第4期943-954,共12页
We used fifteen years (1993-2007) of altimetric data, combined from different missions (ERS-1/2, TOPEX/Poseidon, Jason-l, and Envisat), to analyze the variability of the eddy kinetic energy (EKE) in the South Ch... We used fifteen years (1993-2007) of altimetric data, combined from different missions (ERS-1/2, TOPEX/Poseidon, Jason-l, and Envisat), to analyze the variability of the eddy kinetic energy (EKE) in the South China Sea (SCS). We found that the EKE ranged from 64 cm2/s2 to 1 390 cm2/s2 with a mean value of 314 cm2/s2. The highest EKE center was observed to the east of Vietnam (with a mean value of 509 cm2/s2) and the second highest EKE region Was located to the southwest of Taiwan Island (with a mean value of 319 cm2/s2). We also found that the EKE structure is the consequence of the superposition of different variability components. First, interannual variability is important in the SCS. Spectral analysis of the EKE interannual signal (IA-EKE) shows that the main periodicities of the IA-EKE to the east of Vietuam, to the southwest of Taiwan Island, and in the SCS are 3.75, 1.87, and 3.75 years, respectively. It is to the south of Taiwan Island that the IA-EKE signal has the most obvious impact on EKE variability. In addition, the IA-EKE exhibit different trends in different regions. An obvious positive trend is observed along the east coast of Vietnam, while a negative trend is found to the southwest of Taiwan Island and in the east basin of Vietnam. Correlation analysis shows that the IA-EKE has an obvious negative correlation with the SSTA in Nifio3 (5°S-5°N, 90°W-150°W). E1 Nifio-Southem Oscillation (ENSO) affects the 1A-EKE variability in the SCS through an atmospheric bridge--wind stress curl over the SCS. Second, the seasonal cycle is the most obvious timescale affecting EKE variability. The locations of the most remarkable EKE seasonal variabilities in the SCS are to the east of Vietnam, to the southwest of Taiwan, and to the west of Philippines. To the east of Vietnam, the seasonal cycle is the dominant mechanism controlling EKE variability, which is attributed primarily to the annual cycle there of wind stress curl. In this area, the maximum EKE is observed in autumn. To the southwest of Taiwan Island, the EKE is enlarged by the stronger SCS circulation, which is caused by the intrusion branch from the Kuroshio in winter. Finally, intra-annual and mesoscale variability, although less important than the former, cannot be neglected. The most obvious intra-annual and mesoscale variability, which may be the result of baroclinic instability of the background flow, are observed to the southwest of Taiwan Island. Sporadic events can have an important effect on EKE variability. 展开更多
关键词 ALTIMETRY eddy kinetic energy annual and interannual variability SCS
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Measurement and analysis of soil nitrogen and organic matter content using near-infrared spectroscopy techniques 被引量:8
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作者 何勇 宋海燕 +1 位作者 PEREIRA Annia García GóMEZ Antihus Hernández 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE EI CAS CSCD 2005年第11期1081-1086,共6页
Near infrared reflectance (N1R) spectroscopy is as a rapid, convenient and simple nondestructive technique useful for quantifying several soil properties. This method was used to estimate nitrogen (N) and organic ... Near infrared reflectance (N1R) spectroscopy is as a rapid, convenient and simple nondestructive technique useful for quantifying several soil properties. This method was used to estimate nitrogen (N) and organic matter (OM) content in a soil of Zhejiang Province, Hangzhou County. A total of 125 soil samples were taken from the field. Ninety-five samples spectra were used during the calibration and cross validation stage. Thirty samples spectra were used to predict N and OM concentration. NIR spectra of these samples were correlated using partial least square regression. The regression coefficients between measured and predicted values of N and OM was 0.92 and 0.93, and SEP (standard error of prediction) were 3.28 and 0.06, respectively, which showed that NIR method had potential to accurately predict these constituents in this soil. The results showed that NIR spectroscopy could be a good tool for precision farming application. 展开更多
关键词 NIR spectroscopy Partial least square Precision farming Soil spatial variability NITROGEN Organic matter
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Heart rate variability: a new tool to predict complications in adult cardiac surgery 被引量:5
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作者 Antonio Nenna Mario Lusini +5 位作者 Cristiano Spadaccio Francesco Nappi Salvatore Matteo Greco Raffaele Barbato Elvio Covino Massimo Chello 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2017年第11期662-668,共7页
Heart rate variability (HRV) refers to the variations between consecutive heartbeats, which depend on the continuous modulation of the sympathetic and parasympathetic branches of the autonomic nervous system. HRV ha... Heart rate variability (HRV) refers to the variations between consecutive heartbeats, which depend on the continuous modulation of the sympathetic and parasympathetic branches of the autonomic nervous system. HRV has been shown to be effective as a predictor of risk after myocardial infarction and an early warning sign of diabetic neuropathy, and in the cardiology setting is now recognized to be a useful tool for risk-stratification after hospital admission and after discharge. Recent evidences suggest that HRV analysis might predict complications even in patients undergoing cardiac surgery, and the present review summarizes the importance of HRV analysis in adult cardiac surgery and the perspectives for HRV use in current clinical practice. Although future larger studies are warranted before HRV can be included into daily clinical practice in adult cardiac surgery, HRV is a novel tool which might detect autonomic instability in the early postoperative phase and during hospital stay, thus predicting or prompt-diagnosing many of the post-operative complications. 展开更多
关键词 Cardiac surgery COMPLICATIONS Heart rate variability Predictive values
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Linkage between the Atlantic Tropical Hurricane Frequency and the Antarctic Oscillation in the Western Hemisphere 被引量:6
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作者 FAN Ke 《Atmospheric and Oceanic Science Letters》 2009年第3期159-164,共6页
To examine the zonal asymmetry of the Antarctic oscillation (AAO), different portions of the AAO from June to October (JJASO) in the interannual variability of the Atlantic tropical hurricanes number (ATHN) are docume... To examine the zonal asymmetry of the Antarctic oscillation (AAO), different portions of the AAO from June to October (JJASO) in the interannual variability of the Atlantic tropical hurricanes number (ATHN) are documented in this research. It follows that the AAO in the Western Hemisphere (AAOWH) is positively correlated with the ATHN, at 0.36 during the period of 1871-1998 and 0.42 during the period of 1949-98. After removing the linear regressions on the Southern Oscillation Index (SOI) in all time series, the above correlation coefficients are 0.25 and 0.30, respectively. The underlying mechanisms are studied through analyses of the atmospheric general circulation variability associated with the AAOWH. It turns out that the positive (negative) phase of JJASO AAOWH corresponds with several factors: decreased (increased) vertical zonal wind shear magnitude, low-level anomalous convergence (divergence), high-level anomalous divergence (convergence), and warmed (cooled) sea surface temperature in the tropical Atlantic. Therefore, the positive (negative) phase of JJASO AAOWH is favorable (unfavorable) to the tropical hurricane genesis. 展开更多
关键词 Atlantic hurricanes Antarctic Oscillation in Western Hemisphere Atmospheric Circulation
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Interannual variability of the southern Yellow Sea Cold Water Mass 被引量:6
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作者 胡敦欣 王庆业 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2004年第3期231-236,共6页
Temperature data collected in the sections of 34°N, 35°N and 36°N in August from 1975 through2003 were analyzed using Empirical Orthogonal Function (EOF) to investigate interannual variability of the so... Temperature data collected in the sections of 34°N, 35°N and 36°N in August from 1975 through2003 were analyzed using Empirical Orthogonal Function (EOF) to investigate interannual variability of the southern Yellow Sea Cold Water Mass (YSCWM). The first mode (EOF1) reveals variations of basin-wide thermocline depth, which is mainly caused by surface heating. The second mode (EOF2) presents fluctuations of vertical circulation, resulting mainly from interannual variability of cold front intensity. In addition, it is found that the upward extent of upwelling in the cold front is basically determined by wind stress curl and the zonal position of the warm water center in the southern Yellow Sea is correlated with spatial difference of net heat flux. 展开更多
关键词 YSCWM EOF interannual variability
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Applicability and variability of liver stiffness measurements according to probe position 被引量:5
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作者 Patrick Ingiliz Kim Pav Chhay +7 位作者 Mona Munteanu Pascal Lebray Yen Ngo Dominique Roulot Yves Benhamou Dominique Thabut Vlad Ratziu Thierry Poynard 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第27期3398-3404,共7页
AIM: To investigate the liver stiffness measurement (LSM) applicability and variability with reference to three probe positions according to the region of liver biopsy. METHODS: The applicability for LSM was defined a... AIM: To investigate the liver stiffness measurement (LSM) applicability and variability with reference to three probe positions according to the region of liver biopsy. METHODS: The applicability for LSM was defined as at least 10 valid measurements with a success rate greater than 60% and an interquartile range/median LSM < 30%. The LSM variability compared the inter-position concordance and the concordance with FibroTest. RESULTS: Four hundred and forty two consecutive patients were included. The applicability of the anterior position (81%) was significantly higher than that of the reference (69%) and lower positions (68%), (both P = 0.0001). There was a signif icant difference (0.5 kPa, 95% CI 0.13-0.89; P < 0.0001) between mean LSM estimated at the reference position (9.3 kPa) vs the anterior position (8.8 kPa). Discordance between positions was associated with thoracic fold (P = 0.008). The discordance rate between the reference position result and FibroTest was higher when the 7.1 kPa cutoff was used to define advanced fibrosis instead of 8.8 kPa (33.6% vs 23.5%, P = 0.03).CONCLUSION: The anterior position of the probe should be the fi rst choice for LSM using Fibroscan, as it has a higher applicability without higher variability compared to the usual liver biopsy position. 展开更多
关键词 FIBROSCAN Fibrotest Liver fibrosis VARIABILITY CONCORDANCE
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Spatial and seasonal variation in soil respiration along a slope in a rubber plantation and a natural forest in Xishuangbanna, Southwest China 被引量:3
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作者 ZHAO Yong-li Stefanie D.GOLDBERG +1 位作者 XU Jian-chu Rhett D.HARRISON 《Journal of Mountain Science》 SCIE CSCD 2018年第4期695-707,共13页
Soil respiration is a key component of the global carbon cycle, and even small changes in soil respiration rates could result in significant changes in atmospheric CO_2 levels. The conversion of tropical forests to ru... Soil respiration is a key component of the global carbon cycle, and even small changes in soil respiration rates could result in significant changes in atmospheric CO_2 levels. The conversion of tropical forests to rubber plantations in SE Asia is increasingly common, and there is a need to understand the impacts of this land-use change on soil respiration in order to revise CO_2 budget calculations. This study focused on the spatial variability of soil respiration along a slope in a natural tropical rainforest and a terraced rubber plantation in Xishuangbanna, Southwest(SW) China. In each land-use type, we inserted 105 collars for soil respiration measurements.Research was conducted over one year in Xishuangbanna during May, June, July and October 2015(wet season) and January and March 2016(dry season). The mean annual soil respiration rate was 30% higher in natural forest than in rubber plantation and mean fluxes in the wet and dry season were 15.1 and 9.5 Mg C ha^(-1) yr^(-1) in natural forest and 11.7 and 5.7 Mg C ha^(-1) yr^(-1) in rubber plantation. Using a linear mixedeffects model to assess the effect of changes in soil temperature and moisture on soil respiration, we found that soil temperature was the main driver of variation in soil respiration, explaining 48% of its seasonal variation in rubber plantation and 30% in natural forest. After including soil moisture, the model explained 70% of the variation in soil respiration in natural forest and 76% in rubber plantation. In the natural forest slope position had a significant effect on soil respiration, and soil temperature and soil moisture gradients only partly explained this correlation. In contrast, soil respiration in rubber plantation was not affected by slope position, which may be due to the terrace structure that resulted in more homogeneous environmental conditions along the slope. Further research is needed to determine whether or not these findings hold true at a landscape level. 展开更多
关键词 Soil respiration Tropical rain forest Rubber plantation Land-use change Carbon cycle TRANSECT
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Spatial Variability of Mass Movements in the Satluj Valley,Himachal Pradesh during 1990~2006 被引量:2
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作者 Vikram GUPTA M.P.SAH 《Journal of Mountain Science》 SCIE CSCD 2008年第1期38-51,共14页
Satluj Valley is known to have a history of landslides and related mass movement activities since the geological times. Geological and geomorphological settings combined with anthropogenic activities constitute a prop... Satluj Valley is known to have a history of landslides and related mass movement activities since the geological times. Geological and geomorphological settings combined with anthropogenic activities constitute a propensity towards slope failure. During the last two decades,the area witnessed substantial increase in athropogenic pressure,mainly due to the exploitation of hydropower potential,changing landuse pattern and population growth. In addition,a shift of the climatic patterns in the form of larger area falling under the influence of rains was observed. These natural as well as anthropogenic changes in the area have resulted in increased spatial coverage of landslide in the area. This paper documents these changes during 1990~2006. 展开更多
关键词 LANDSLIDES Mass movement Satluj Valley Himachal Pradesh INDIA
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Response of North Pacific and North Atlantic decadal variability to weak global warming 被引量:5
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作者 WU Sheng LIU Zheng-Yu +1 位作者 CHENG Jun LI Chun 《Advances in Climate Change Research》 SCIE CSCD 2018年第2期95-101,共7页
The Pacific Decadal Oscillation (PDO) and the Atlantic Multidecadal Variability (AMV) are the two dominant low-frequency modes in the climate system. This research focused on the response of these two modes under ... The Pacific Decadal Oscillation (PDO) and the Atlantic Multidecadal Variability (AMV) are the two dominant low-frequency modes in the climate system. This research focused on the response of these two modes under weak global warming. Observational data were derived from the Hadley Center Sea Ice and Sea Surface Temperature dataset (HadISST) and coupled model outputs from the Coupled Model Intercomparison Project Phase 5 (CMIP5). Changes in PDO and AMV were examined using four models (bcc-csml-1, CCSM4, IPSL-CM5A-LR, and MPI- ESM-LR) with long weak global warming scenarios (RCP2.6). These models captured the two low-frequency modes in both pre-industrial run and RCP2.6 run. Under weak global warming, the time scales of PDO and AMV significantly decreased while the amplitude only slightly decreased. Interestingly, the standard deviation of the North Pacific sea surface temperature anomaly (SSTA) decreased only in decadal time scale, and that of the North Atlantic SSTA decreased both in interannual and decadal time scales. The coupled system consists of a slow ocean component, which has a decadal time scale, and a fast atmospheric component, which is calculated by subtracting the decadal from the total. Results suggest that under global warming, PDO change is dominated by ocean dynamics, and AMV change is dominated by ocean dynamics and stochastic atmosphere forcing. 展开更多
关键词 Pacific decadal oscillation Atlantic multidecadal variability RCP2.6
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Expression and identification of recombinant soluble single-chain variable fragment of monoclonal antibody MC3 被引量:13
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作者 Feng-Tian He Rong-Fen Li Yun-Sheng Kang Yan Zhang,Department of Biochemistry & Molecular Biology,Third Military Medical University,Chongqing 400038,China Yong-Zhan Nie Bao-Jun Chen Tai-Dong Qiao Dai-Ming Fan,Institute of Digestive Disease,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2002年第2期258-262,共5页
AIM: To generate soluble single chain variable fragments (ScFv) of monoclonal antibody MC3 recognizing colorectal and gastric carcinomas. METHODS: mRNA was isolated from the hybridoma cell line producing MC3 and the D... AIM: To generate soluble single chain variable fragments (ScFv) of monoclonal antibody MC3 recognizing colorectal and gastric carcinomas. METHODS: mRNA was isolated from the hybridoma cell line producing MC3 and the DNAs encoding variable domains of heavy and light chains (VH and VL) of the antibody were amplified separately by RT-PCR and assembled into ScFv DNA with a linker DNA. The ScFv DNA was ligated into the phagemid vector pCANTAB5E and the ligated sample was transformed into E.coli TG1.The transformed cells were infected with M13KO7 helper phage to yield recombinant phages. After two rounds of panning with gastric carcinoma cell line AGS highly expressing MC3-binding antigen, the phage clones displaying ScFv fragments of the antibody were selected by ELISA. 4 phage clones showing strong signal in ELISA were used to infect E.coli HB2151 to express soluble ScFvs. The soluble ScFvs were identified by Dot blot and Western blot, and their antigen-binding activity was assayed by ELISA. The VH and VL DNAs of the ScFv DNA derived from phage clone 19 were sequenced. RESULTS: The VH,VL and ScFv DNAs were about 340 bp, 320 bp and 750 bp respectively. After two rounds of panning to the recombinant phages, 18 antigen-positive phage clones were selected from 30 preselected phage clones by ELISA. All the soluble ScFvs derived from the 4 out of the 18 antigen-positive phage clones were about M(r)32000 and concentrated in periplasmatic space under the given culture condition. The soluble ScFvs could bind the antigen, and they shared the same binding site with MC3. The sequences of the VH and VL DNAs of the MC3 ScFv showed that the variable antibody genes belonged to the IgG1 subgroup,kappa-type. CONCLUSION: The soluble ScFv of MC3 is successfully produced, which not only provides a possible novel targeting vehicle for in vivo and in vitro study on associated cancers, but also offers the antibody a stable genetic source. 展开更多
关键词 Animals Antibodies Monoclonal Base Sequence Carcinoma Colorectal Neoplasms Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin Fragments Immunoglobulin Variable Region Mice Molecular Sequence Data Recombinant Proteins Stomach Neoplasms Tumor Cells Cultured
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Variability of the Continental Water Boundary near the Prydz Bay 被引量:3
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作者 蒲书箴 董兆乾 +2 位作者 胡筱敏 于非 赵新 《Marine Science Bulletin》 CAS 2002年第1期1-10,共10页
Based on the data obtained during the 15th Antarctic Expedition of China between November 1998 and February 1999, the paper discusses the water masses distributed on both sides of the Continental Water Boundary (CWB) ... Based on the data obtained during the 15th Antarctic Expedition of China between November 1998 and February 1999, the paper discusses the water masses distributed on both sides of the Continental Water Boundary (CWB) as well as the spacial variability of the physical characteristics of the central location, the frontal width, the frontal strength, the vertical depth, and the vertical thickness for CWB. The above results are compared with the results from the data obtained during the 9th Antarctic Expedition of China between December 1992 and February 1993, and the temporal variability of those characteristics is discussed. In addition, the inhomogeneity of the kinematic characteristics and the patterns of the dynamic heights for those water masses located on both sides of CWB are illustrated. As it is pointed out in the paper, the trough in the dynamic height field is identified with the frontal location of CWB and the oceanic current shear. Furthermore, the dynamic reason for the upwelling of the circumpolar deep water can be understood by the explanation described in the paper. 展开更多
关键词 Pryze Bay oceanic front Continental Water Boundary VARIABILITY
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Drought and Spatiotemporal Variability of Forest Fires Across Mexico 被引量:3
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作者 Pompa-García MARíN Camarero J.JULIO +1 位作者 Rodríguez-Trejo DANTE ARTURO Vega-Nieva DANIEL JOSE 《Chinese Geographical Science》 SCIE CSCD 2018年第1期25-37,共13页
Understanding the spatiotemporal links between drought and forest fire occurrence is crucial for improving decision-making in fire management under current and future climatic conditions. We quantified forest fire act... Understanding the spatiotemporal links between drought and forest fire occurrence is crucial for improving decision-making in fire management under current and future climatic conditions. We quantified forest fire activity in Mexico using georeferenced fire records for the period of 2005–2015 and examined its spatial and temporal relationships with a multiscalar drought index, the Standardized Precipitation-Evapotranspiration Index(SPEI). A total of 47975 fire counts were recorded in the 11-year long study period, with the peak in fire frequency occurring in 2011. We identified four fire clusters, i.e., regions where there is a high density of fire records in Mexico using the Getis-Ord G spatial statistic. Then, we examined fire frequency data in the clustered regions and assessed how fire activity related to the SPEI for the entire study period and also for the year 2011. Associations between the SPEI and fire frequency varied across Mexico and fire-SPEI relationships also varied across the months of major fire occurrence and related SPEI temporal scales. In particular, in the two fire clusters located in northern Mexico(Chihuahua, northern Baja California), drier conditions over the previous 5 months triggered fire occurrence. In contrast, we did not observe a significant relationship between drought severity and fire frequency in the central Mexico cluster, which exhibited the highest fire frequency. We also found moderate fire-drought associations in the cluster situated in the tropical southern Chiapas where agriculture activities are the main causes of forest fire occurrence. These results are useful for improving our understanding of the spatiotemporal patterns of fire occurrence as related to drought severity in megadiverse countries hosting many forest types as Mexico. 展开更多
关键词 cluster DROUGHT forest fires GEOSTATISTICS spatial clusters Standardised Precipitation-Evapotranspiration Index (SPEI)
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