Chinese and Japanese population of Chlamys farreri and their reciprocal hybrids were surveyed in isozyme variability at 13 loci by polyacrytamide gel electrophoresis (PAGE). Isozyme banding patterns indicated these hy...Chinese and Japanese population of Chlamys farreri and their reciprocal hybrids were surveyed in isozyme variability at 13 loci by polyacrytamide gel electrophoresis (PAGE). Isozyme banding patterns indicated these hybrids were diploid. Loci that were observed as being monomorphic in inbred populations of C. farreri were also found to be monomorphic in filial progeny; loci that observed to be polymorphic in parental type gen- erations were also polymorphic in hybrid generations. Differences existed among allelic frequency of the four types of cross. Within the reciprocal hybrids the expression of malic enzyme (ME) isozyme was sufficient to distinguishing individual hybrids because of the band, Rf=0.38. However, there were no noticeable variations among all the samples to differentiate one from another. Inbreeding was likely to be the main problem in aqua- culture. The introduction of new broodstock can improve the genetic diversity. Hybrid vigor has manifested to a certain extent in the present study.展开更多
Background:Hepatocellular carcinoma(HCC)is frequently associated withmetabolism dysfunction.Increasing evidence has demonstrated the crucial role of lipidmetabolismin HCC progression.The function of apolipoprotein F(A...Background:Hepatocellular carcinoma(HCC)is frequently associated withmetabolism dysfunction.Increasing evidence has demonstrated the crucial role of lipidmetabolismin HCC progression.The function of apolipoprotein F(ApoF),a lipid transfer inhibitor protein,in HCC is incompletely understood.We aimed to evaluate the functional role of ApoF in HCC in this study.Methods:We used quantitative reverse-transcription polymerase chain reaction(qRT-PCR)to detect ApoF mRNA expression in HCC tissues and hepatoma cell lines(SMMC-7721,HepG2,and Huh7).Immunohistochemistry was performed to detect the expression of ApoF in HCC tissues.The associations between ApoF expression and clinicopathological features as well as HCC prognosis were analyzed.The effect of ApoF on cellular proliferation and growth of SMMC-7721 and Huh7 cells was examined in vitro and in vivo.Results:ApoF expression was significantly down-regulated at both mRNA and protein levels in HCC tissues as compared with adjacent tissues.In SMMC-7721 and Huh7 HCC cells,ApoF overexpression inhibited cell proliferation and migration.In a xenograft nude mouse model,ApoF overexpression effectively controlled HCC growth.Kaplan–Meier analysis results showed that the recurrence-free survival rate of HCC patients with low ApoF expression was significantly lower than that of other HCC patients.Low ApoF expression was associated with several clinicopathological features such as liver cirrhosis,Barcelona Clinic Liver Cancer stage and tumor-node-metastasis stage.Conclusions:ApoF expression was down-regulated in HCC,which was associated with low recurrence-free survival rate.ApoF may serve as a tumor suppressor in HCC and be a potential application for the treatment of this disease.展开更多
基金Supported by the National "973" Program (G1999012008) and "863" Program (2002AA626020).
文摘Chinese and Japanese population of Chlamys farreri and their reciprocal hybrids were surveyed in isozyme variability at 13 loci by polyacrytamide gel electrophoresis (PAGE). Isozyme banding patterns indicated these hybrids were diploid. Loci that were observed as being monomorphic in inbred populations of C. farreri were also found to be monomorphic in filial progeny; loci that observed to be polymorphic in parental type gen- erations were also polymorphic in hybrid generations. Differences existed among allelic frequency of the four types of cross. Within the reciprocal hybrids the expression of malic enzyme (ME) isozyme was sufficient to distinguishing individual hybrids because of the band, Rf=0.38. However, there were no noticeable variations among all the samples to differentiate one from another. Inbreeding was likely to be the main problem in aqua- culture. The introduction of new broodstock can improve the genetic diversity. Hybrid vigor has manifested to a certain extent in the present study.
基金This study was supported by grants from the National Natural Science Foundation of China[No.81572726]the Natural Science Foundation of Guangdong Province[No.2018A030313641 and No.2016A030313848]+1 种基金the Science and Technology Planning Project of Guangdong Province[No.2014A020212122 and No.2016A020212004]the Medical Research Foundation of Guangdong Province[No.A2016312].
文摘Background:Hepatocellular carcinoma(HCC)is frequently associated withmetabolism dysfunction.Increasing evidence has demonstrated the crucial role of lipidmetabolismin HCC progression.The function of apolipoprotein F(ApoF),a lipid transfer inhibitor protein,in HCC is incompletely understood.We aimed to evaluate the functional role of ApoF in HCC in this study.Methods:We used quantitative reverse-transcription polymerase chain reaction(qRT-PCR)to detect ApoF mRNA expression in HCC tissues and hepatoma cell lines(SMMC-7721,HepG2,and Huh7).Immunohistochemistry was performed to detect the expression of ApoF in HCC tissues.The associations between ApoF expression and clinicopathological features as well as HCC prognosis were analyzed.The effect of ApoF on cellular proliferation and growth of SMMC-7721 and Huh7 cells was examined in vitro and in vivo.Results:ApoF expression was significantly down-regulated at both mRNA and protein levels in HCC tissues as compared with adjacent tissues.In SMMC-7721 and Huh7 HCC cells,ApoF overexpression inhibited cell proliferation and migration.In a xenograft nude mouse model,ApoF overexpression effectively controlled HCC growth.Kaplan–Meier analysis results showed that the recurrence-free survival rate of HCC patients with low ApoF expression was significantly lower than that of other HCC patients.Low ApoF expression was associated with several clinicopathological features such as liver cirrhosis,Barcelona Clinic Liver Cancer stage and tumor-node-metastasis stage.Conclusions:ApoF expression was down-regulated in HCC,which was associated with low recurrence-free survival rate.ApoF may serve as a tumor suppressor in HCC and be a potential application for the treatment of this disease.
