In order to improve the accuracy for quantitating the bovine foamy virus (BFV) in vitro,we developed a baby hamster kidney cell (BHK)-21-derived indicator cell line containing a plasmid that encodes the firefly lucife...In order to improve the accuracy for quantitating the bovine foamy virus (BFV) in vitro,we developed a baby hamster kidney cell (BHK)-21-derived indicator cell line containing a plasmid that encodes the firefly luciferase driven by the BFV long terminal repeat promoter (LTR,from-7 to 1012).The BFV titer could be determined by detecting the luciferase expression since the viral trans-activator BTas protein activates the promoter activity of the LTR.One clone,designated BFVL,was selected from ten neomycin-resistant clones.BFVL showed a specific and inducible dose-and time-dependent luciferase activity in response to BFV infection.Although the changes in luciferase activity of BFVL peaked at 84 h post infection,it was possible to differentiate infected and uninfected cells at 48 h post infection.A linear relationship was established between the multiplicity of infection (MOI) of BFV and the activated ratio of luciferase expression in BFVL.Moreover,the sensitivity of the BFVL-based assay for detecting infectious BFV was 10,000 times higher than the conventional CPE-based assay at 48 h post infection.These findings suggest that the BFVL-based assay is rapid,easy,sensitive,quantitative and specific for detection of BFV infection.展开更多
My paper aims to discuss the approach between the mystic＇s experience and the experiment of the scientist. The mystic experience is closer to what is proper to the scientific experiment： both are, finally, ways of t...My paper aims to discuss the approach between the mystic＇s experience and the experiment of the scientist. The mystic experience is closer to what is proper to the scientific experiment： both are, finally, ways of tryout. This also means that both are ways to come closer to a deeper understanding of Reality. Raising the problem of the relation between religion and science requires a series of precautions, especially related to the underlying meanings of what we call ＂religion＂ or ＂science＂. What is meant by religion and religious？ The terms are too vague, and on the other hand, the differences among religions are so important that it is almost impossible to use the term religion without an abstract meaning. Even the overall invocation of Christianity is improper, as the report of Eastern Christianity to the Western, one reveals different perspectives on some issues that determine the relationship with science. There must be also taken into account the history of the religious doctrines after Enlightenment, as they underwent secular influences that sometimes left tracks in their discourse, the most aggressive factor being the ideological one. And when speaking about the possibility of religion getting open to science it is necessary to specify what type of religious discourse is concerned. Since a generic report of religion to science is slippery, we should rather count on the identification of those types of religious experience and of those religious horizons that can offer a real openness to science. These statements are also true for science： for such a dialogue, there is need of identifying those aspects of science that are not contaminated by ideology or by assumptions alien to its experimental nature.展开更多
基金National Natural Science Foundation of China(31070135,81071343)
文摘In order to improve the accuracy for quantitating the bovine foamy virus (BFV) in vitro,we developed a baby hamster kidney cell (BHK)-21-derived indicator cell line containing a plasmid that encodes the firefly luciferase driven by the BFV long terminal repeat promoter (LTR,from-7 to 1012).The BFV titer could be determined by detecting the luciferase expression since the viral trans-activator BTas protein activates the promoter activity of the LTR.One clone,designated BFVL,was selected from ten neomycin-resistant clones.BFVL showed a specific and inducible dose-and time-dependent luciferase activity in response to BFV infection.Although the changes in luciferase activity of BFVL peaked at 84 h post infection,it was possible to differentiate infected and uninfected cells at 48 h post infection.A linear relationship was established between the multiplicity of infection (MOI) of BFV and the activated ratio of luciferase expression in BFVL.Moreover,the sensitivity of the BFVL-based assay for detecting infectious BFV was 10,000 times higher than the conventional CPE-based assay at 48 h post infection.These findings suggest that the BFVL-based assay is rapid,easy,sensitive,quantitative and specific for detection of BFV infection.
文摘My paper aims to discuss the approach between the mystic＇s experience and the experiment of the scientist. The mystic experience is closer to what is proper to the scientific experiment： both are, finally, ways of tryout. This also means that both are ways to come closer to a deeper understanding of Reality. Raising the problem of the relation between religion and science requires a series of precautions, especially related to the underlying meanings of what we call ＂religion＂ or ＂science＂. What is meant by religion and religious？ The terms are too vague, and on the other hand, the differences among religions are so important that it is almost impossible to use the term religion without an abstract meaning. Even the overall invocation of Christianity is improper, as the report of Eastern Christianity to the Western, one reveals different perspectives on some issues that determine the relationship with science. There must be also taken into account the history of the religious doctrines after Enlightenment, as they underwent secular influences that sometimes left tracks in their discourse, the most aggressive factor being the ideological one. And when speaking about the possibility of religion getting open to science it is necessary to specify what type of religious discourse is concerned. Since a generic report of religion to science is slippery, we should rather count on the identification of those types of religious experience and of those religious horizons that can offer a real openness to science. These statements are also true for science： for such a dialogue, there is need of identifying those aspects of science that are not contaminated by ideology or by assumptions alien to its experimental nature.
