Implementation of simultaneous execution phases in the concurrent engineering (CE) needs careful planning when the downstream phase could be activated as the upstream phase developed to a certain point. The determinat...Implementation of simultaneous execution phases in the concurrent engineering (CE) needs careful planning when the downstream phase could be activated as the upstream phase developed to a certain point. The determination of startup time of overlapping jobs in CE has long been a disturbance in manufacturing industry implementing CE programs. A novel model based on both fuzzy logic and neural network is proposed to mathematically formulate the inter-connective information between the two coupled phases in CE projects, and to determine the startup time of downstream phases in real time. The information transferring between the two coupled phases is quantified by using the negative Shannon entropy. Based on this algorithm, a PDM-based framework is proposed to narrow the gap between pro-duct design and manufacture, in which five modules are built to monitor, reshuffle and implement the simultaneous executing processes. Finally, an example is given to illustrate applications of the algorithm in the real world.展开更多
This paper presented a novel bus encoding method to reduce the switching activity on address buses and hence reduce power dissipation. Dynamic-sorting encoding(DSE) method reduces the power dissipation of address bus ...This paper presented a novel bus encoding method to reduce the switching activity on address buses and hence reduce power dissipation. Dynamic-sorting encoding(DSE) method reduces the power dissipation of address bus based on the dynamic reordering of the modified offset address bus lines. This method reorders the ten least significant bits of offset address according to the range of offset address, and the optimal sorting pattern is transmitted through the high bits of address bus without the need for redundant bus lines. The experimental results by using an instruction set simulator and SPEC2000 benchmarks show that DSE method can reduce signal transitions on the address bus by 88.2%, and the actual overhead of the encoder circuit is estimated after encoder is designed and synthesized in 0.18-μm CMOS technology. The results show that DSE method outperforms the low-power encoding schemes presented in the past.展开更多
In this note,by combining the nice numerical performance of PR and HS methods with the global convergence property of FR method,a class of new restarting three terms conjugate gradient methods is presented.Global conv...In this note,by combining the nice numerical performance of PR and HS methods with the global convergence property of FR method,a class of new restarting three terms conjugate gradient methods is presented.Global convergence properties of the new method with two kinds of common line searches are proved.展开更多
Analyzed working principle and starting process of asynchronous motor direct torque control system. In order to solve the problem of serious fluctuation of starting current in traditional control scheme, the author pu...Analyzed working principle and starting process of asynchronous motor direct torque control system. In order to solve the problem of serious fluctuation of starting current in traditional control scheme, the author puts forward a new kind of starting method which can limit voltage value to achieve the goal of reducing fluctuation of the current by increasing zero voltage vectors during the stage of starting. The author has also carried on simulation study on the system, and the result of simulation study shows that this method is effectual.展开更多
In this paper, operation characteristic of condensate polishing system is studied during startup, based on generator blowdown limit, given limit ion concentration in condensate during condenser leakage. Further give c...In this paper, operation characteristic of condensate polishing system is studied during startup, based on generator blowdown limit, given limit ion concentration in condensate during condenser leakage. Further give conclusion about condensate polishing system configuration and operation requirement: full flow condensate polishing system (CPS) is necessary to the units cooled by sea water, and it is better that one or two series put in service during normal operation other than all of them in hot standby. While continuous condensate tube leakage is 256 L/h, the units cooled by fresh water will be shutdown immediately, for this level leakage, whichever condensate polishing system capacity is 50% (30%) or 100%, action level 2 will be preformed, so 50% (30%) is enough for fresh water cooled units.展开更多
The stronglink with muhi-try function based on MEMS technology and the PC startup in authentication system have been designed and fabricated. The generation principle and structure of UQS code are introduced, which co...The stronglink with muhi-try function based on MEMS technology and the PC startup in authentication system have been designed and fabricated. The generation principle and structure of UQS code are introduced, which consists of two groups of metal counter-meshing gears, two pawl/ratchet mechanisms, two driving micromotors and two resetting micromotors. The energy-coupling element is a photoelectric sensor with a circular and notched plate. It is fabricated using the UV-LiGA process and precision mechanical engineering. The PC startup authentication system is controlled by BIOS program, which is written into the chip according with special format. The program in BIOS output signals controls the running of stronglink to finish the process of authentication. The device can run more than 10000 times before a stop. The driving voltage is 12 V, and the normal decoding time is 3 s.展开更多
In this article, the 2D transient FEM has been used to analyze the dynamic process of the three phase LS-PMSM running characteristic. By verifying it through the experiment, we obtain the conclusion that the simulatio...In this article, the 2D transient FEM has been used to analyze the dynamic process of the three phase LS-PMSM running characteristic. By verifying it through the experiment, we obtain the conclusion that the simulation result is consistent with the experiment result. At the same time, by analyzing the situation of the magnetic bridge, we also obtain the result that the EMF waves are changing with the situation of the magnetic poles on and we can optimize the motor’s structure at the same time.展开更多
Control of the fed-batch ethanol fermentation processes to produce maximum product ethanol is one of the key issues in the bioreactor system.However,ethanol fermentation processes exhibit complex behavior and nonlinea...Control of the fed-batch ethanol fermentation processes to produce maximum product ethanol is one of the key issues in the bioreactor system.However,ethanol fermentation processes exhibit complex behavior and nonlinear dynamics with respect to the cell mass,substrate,feed-rate,etc.An improved dual heuristic programming algorithm based on the least squares temporal difference with gradient correction(LSTDC) algorithm(LSTDC-DHP) is proposed to solve the learning control problem of a fed-batch ethanol fermentation process.As a new algorithm of adaptive critic designs,LSTDC-DHP is used to realize online learning control of chemical dynamical plants,where LSTDC is commonly employed to approximate the value functions.Application of the LSTDC-DHP algorithm to ethanol fermentation process can realize efficient online learning control in continuous spaces.Simulation results demonstrate the effectiveness of LSTDC-DHP,and show that LSTDC-DHP can obtain the near-optimal feed rate trajectory faster than other-based algorithms.展开更多
In light of the possibility of reignition when unsealing the closed fire zones, the occurrence of reignition was simulated by a home-made experimental device, hydrogen variety rule from combustion to unsealing was ana...In light of the possibility of reignition when unsealing the closed fire zones, the occurrence of reignition was simulated by a home-made experimental device, hydrogen variety rule from combustion to unsealing was analyzed, the three-stage generation model of hydrogen during reignition in fire zone was put forward and the probability that hydrogen was taken as an indicator gas was discussed. The results show that: when fire zone is ready to unseal, which is filled with hydrogen at a high concentration, it is more prone to reignite.展开更多
Classical management accounting (MA) Focusing on the facilitating perspective, focuses on decision facilitating and influencing (Demski & Feltham, 1976). MA has to provide information to managers and depending on...Classical management accounting (MA) Focusing on the facilitating perspective, focuses on decision facilitating and influencing (Demski & Feltham, 1976). MA has to provide information to managers and depending on the problem complexity, they have to solve problems in a dyadic way. A dual process model, the heuristic systematic model (HSM), expands this so-called manager-accountant-dyad and shows different cases of actual human information processing. Managers and accountants either process systematically or heuristically. So far, many concepts have been designed in relation to the normative concept of the economical rational principle. Consequently, recent research only uses systematic information processing, based on the principle of the economic man. In this paper, a decision-behavior oriented approach tries to describe actual decision makers such as managers and accountants and shows new possibilities within MA. Therefore, the potential of heuristic information processing is analyzed, based on the phenomenon of ecological rationality as one shape of bounded rationality. Thus, different cognitive heuristics in business economics are identified and analyzed. Furthermore, the outstanding performance of heuristics compared with more complex calculations is shown. Unfortunately, these findings have been limited to marketing and investments so far. Significant research is needed, regarding conditions for applications and success factors of heuristics in business economics. New empirical findings have to be explicitly transferred to MA.展开更多
This article mainly introduces the multi-layer distributed C/S architecture of system design scheme. Its working principle is the client program runs automatically after the computer starts, and establish communicatio...This article mainly introduces the multi-layer distributed C/S architecture of system design scheme. Its working principle is the client program runs automatically after the computer starts, and establish communication with the application server. The network administrator can monitor and intelligent management of the client computer through the server program, the computer will execute the corresponding operation according to the server to send command instructions. The system realize the main module of the whole system framework, network monitoring data initialization module, network data transmission module, image coding and decoding module, the advantages of system make full use of existing LAN resources, timely delivery and manaRement information.展开更多
The scramjet and maglev engineering technology development and trends at home and abroad are firstly presented in this paper. A new launch mode of space transportation system is proposed based on scramjet and magnetic...The scramjet and maglev engineering technology development and trends at home and abroad are firstly presented in this paper. A new launch mode of space transportation system is proposed based on scramjet and magnetic suspension technologies, and its key technologies required are given. This paper also makes analysis on using scramjet and magnetic suspension technologies to launch a reusable rocket, and the results show that a normal temperature conductor maglev launch system is feasible.展开更多
To address large scale industrial processes,a novel Lagrangian scheme is proposed to decompose a refinery scheduling problem with operational transitions in mode switching into a production subproblem and a blending a...To address large scale industrial processes,a novel Lagrangian scheme is proposed to decompose a refinery scheduling problem with operational transitions in mode switching into a production subproblem and a blending and delivery subproblem.To accelerate the convergence of Lagrange multipliers,some auxiliary constraints are added in the blending and delivery subproblem.A speed-up scheme is presented to increase the efficiency for solving the production subproblem.An initialization scheme of Lagrange multipliers and a heuristic algorithm to find feasible solutions are designed.Computational results on three cases with different lengths of time horizons and different numbers of orders show that the proposed Lagrangian scheme is effective and efficient.展开更多
What language competence a learner has can she/he achieve an effective communication? This question is the main concern for applied linguists and English teachers in TEFL, for the recognition of language competence d...What language competence a learner has can she/he achieve an effective communication? This question is the main concern for applied linguists and English teachers in TEFL, for the recognition of language competence directly influences the goal of teaching, curriculum design, textbook writing and classroom practice. This paper discusses the various points of views on language competence and explores its characteristics which, the author believes, will shed some light on foreign language teaching.展开更多
The flowing mechanism of a low permeability gas reservoir is different from a conventional gas reservoir,especially for that with higher irreducible water saturation the threshold pressure gradient exists. At present,...The flowing mechanism of a low permeability gas reservoir is different from a conventional gas reservoir,especially for that with higher irreducible water saturation the threshold pressure gradient exists. At present,in all the deliverability equation,the additional pressure drop caused by the threshold pressure gradient is viewed as constant,but this method has big error in the practical application. Based on the non-Darcy steady flow equation,the limited integral of the additional pressure drop is solved in this paper and it is realized that the additional pressure drop is not a constant but has something to do with production data,and a new deliverability equation is derived,with the relevant processing method for modified isochronal test data. The new deliverability equation turns out to be practical through onsite application.展开更多
This paper deals with experimental study of flow field of starting process in two-dimensional, single-stage supersonic ejector on different air total pressure. Schlieren pictures of flow field were taken, static press...This paper deals with experimental study of flow field of starting process in two-dimensional, single-stage supersonic ejector on different air total pressure. Schlieren pictures of flow field were taken, static pressure distribu-tions on side wall were measured. The obtained results show that, on critical pressure, the starting main shock waves in ejector oscillated back and forth between the second throat and the middle section of the mixing chamber, it causes the pressure in the second half of the mixing chamber acutely fluctuated .When the working pressure of the active flow is higher than the critical starting pressure, ejector starts normally and the inner flow-field of the mixing chamber keeps stable and the shock waves in the second throat have a certain degree of oscillation . After ejector starts, the operating pressure of the active flow may be lower than the starting pressure .展开更多
To reduce the fuel consumption of internal combustion engines, more attention has been paid to the tribological performance of the piston ring pack during the cold start and idle operations. In this research, a numeri...To reduce the fuel consumption of internal combustion engines, more attention has been paid to the tribological performance of the piston ring pack during the cold start and idle operations. In this research, a numerical model considering the cylinder liner deformation and the piston ring conformability is developed to predict the blow-by, lubrication, friction and wear of the piston ring pack under different operating conditions. The gas flow rate, inter-ring gas pressures, minimum oil film thickness, frictional force and wear load during cold start are calculated and compared with those during warm idle operating conditions. The results show that cylinder liner deformation and piston ring conformability together obviously affect blow-by and other tribological performance. Meanwhile, it is found that friction loss is larger during cold start than during warm idle operating conditions. However, the wear process is more severe during warm idle operation than during cold start. From this research, the blow-by and tribological performance of the piston ring pack during cold start and warm idle operations are understood more deeply.展开更多
White adipocytes play important roles in many physiological processes, including energy storage, endocrine signaling, and inflammatory responses. Understanding the molecular mechanisms of adipocyte formation (adipoge...White adipocytes play important roles in many physiological processes, including energy storage, endocrine signaling, and inflammatory responses. Understanding the molecular mechanisms of adipocyte formation (adipogenesis) provides insights into therapeutic approaches against obesity and its related diseases. Many transcriptional Factors and epigenetic enzymes are known to regulate adipogenesis; however, whether histone variants play a role in this process is unknown. Here we found that macroH2A1.1 (mH2A1.1), a variant of histone H2A, was upregulated during adipocyte differentiation in 3T3-L1 ceils and in the white adipose tissue of obese mice. Ablation of mH2A1.1 activated Wnt/β-catenin signaling pathway, while overexpression of mH2A1.1 showed opposite effects. We farther found that mH2A1.1 regulated Wnt/β-catenin signaling pathway by cooperating with EZH2, a histone H3K27 methyltrans- ferase, thus led to accumulation of H3K27me2 and H3K27me3 on the promoters of Wnt genes. Mutations in the macro-domain, mH2A1.1G224E, and mH2A1.1G314E, not only impaired adipogenesis, but also impaired the binding ability of mH2A1.1 to EZH2 and the enrichments of H3K27me2 and H3K27me3 on the promoters of Wnt genes. Together, our study reveals a novel regulatory role of mH2A1.1 in adipogenesis and obesity, which provides new insights in white fat development.展开更多
文摘Implementation of simultaneous execution phases in the concurrent engineering (CE) needs careful planning when the downstream phase could be activated as the upstream phase developed to a certain point. The determination of startup time of overlapping jobs in CE has long been a disturbance in manufacturing industry implementing CE programs. A novel model based on both fuzzy logic and neural network is proposed to mathematically formulate the inter-connective information between the two coupled phases in CE projects, and to determine the startup time of downstream phases in real time. The information transferring between the two coupled phases is quantified by using the negative Shannon entropy. Based on this algorithm, a PDM-based framework is proposed to narrow the gap between pro-duct design and manufacture, in which five modules are built to monitor, reshuffle and implement the simultaneous executing processes. Finally, an example is given to illustrate applications of the algorithm in the real world.
基金Sponsored by the National High Technology Research and Development Prgram of China(Grant No.2005AA1Z1100).
文摘This paper presented a novel bus encoding method to reduce the switching activity on address buses and hence reduce power dissipation. Dynamic-sorting encoding(DSE) method reduces the power dissipation of address bus based on the dynamic reordering of the modified offset address bus lines. This method reorders the ten least significant bits of offset address according to the range of offset address, and the optimal sorting pattern is transmitted through the high bits of address bus without the need for redundant bus lines. The experimental results by using an instruction set simulator and SPEC2000 benchmarks show that DSE method can reduce signal transitions on the address bus by 88.2%, and the actual overhead of the encoder circuit is estimated after encoder is designed and synthesized in 0.18-μm CMOS technology. The results show that DSE method outperforms the low-power encoding schemes presented in the past.
基金Supported by the National Natural Science Foundation of China(10571106) Supported by the Fundamental Research Funds for the Central Universities(10CX04044A)
文摘In this note,by combining the nice numerical performance of PR and HS methods with the global convergence property of FR method,a class of new restarting three terms conjugate gradient methods is presented.Global convergence properties of the new method with two kinds of common line searches are proved.
文摘Analyzed working principle and starting process of asynchronous motor direct torque control system. In order to solve the problem of serious fluctuation of starting current in traditional control scheme, the author puts forward a new kind of starting method which can limit voltage value to achieve the goal of reducing fluctuation of the current by increasing zero voltage vectors during the stage of starting. The author has also carried on simulation study on the system, and the result of simulation study shows that this method is effectual.
文摘In this paper, operation characteristic of condensate polishing system is studied during startup, based on generator blowdown limit, given limit ion concentration in condensate during condenser leakage. Further give conclusion about condensate polishing system configuration and operation requirement: full flow condensate polishing system (CPS) is necessary to the units cooled by sea water, and it is better that one or two series put in service during normal operation other than all of them in hot standby. While continuous condensate tube leakage is 256 L/h, the units cooled by fresh water will be shutdown immediately, for this level leakage, whichever condensate polishing system capacity is 50% (30%) or 100%, action level 2 will be preformed, so 50% (30%) is enough for fresh water cooled units.
基金Sponsored by the National High Technology Research and Development Program (863 ) of China (Grant No.2003AA404210, 2005AA404250,2003AA404210, 2006AA01Z443)
文摘The stronglink with muhi-try function based on MEMS technology and the PC startup in authentication system have been designed and fabricated. The generation principle and structure of UQS code are introduced, which consists of two groups of metal counter-meshing gears, two pawl/ratchet mechanisms, two driving micromotors and two resetting micromotors. The energy-coupling element is a photoelectric sensor with a circular and notched plate. It is fabricated using the UV-LiGA process and precision mechanical engineering. The PC startup authentication system is controlled by BIOS program, which is written into the chip according with special format. The program in BIOS output signals controls the running of stronglink to finish the process of authentication. The device can run more than 10000 times before a stop. The driving voltage is 12 V, and the normal decoding time is 3 s.
文摘In this article, the 2D transient FEM has been used to analyze the dynamic process of the three phase LS-PMSM running characteristic. By verifying it through the experiment, we obtain the conclusion that the simulation result is consistent with the experiment result. At the same time, by analyzing the situation of the magnetic bridge, we also obtain the result that the EMF waves are changing with the situation of the magnetic poles on and we can optimize the motor’s structure at the same time.
基金Supported by the National Natural Science Foundation of China(61573052)
文摘Control of the fed-batch ethanol fermentation processes to produce maximum product ethanol is one of the key issues in the bioreactor system.However,ethanol fermentation processes exhibit complex behavior and nonlinear dynamics with respect to the cell mass,substrate,feed-rate,etc.An improved dual heuristic programming algorithm based on the least squares temporal difference with gradient correction(LSTDC) algorithm(LSTDC-DHP) is proposed to solve the learning control problem of a fed-batch ethanol fermentation process.As a new algorithm of adaptive critic designs,LSTDC-DHP is used to realize online learning control of chemical dynamical plants,where LSTDC is commonly employed to approximate the value functions.Application of the LSTDC-DHP algorithm to ethanol fermentation process can realize efficient online learning control in continuous spaces.Simulation results demonstrate the effectiveness of LSTDC-DHP,and show that LSTDC-DHP can obtain the near-optimal feed rate trajectory faster than other-based algorithms.
基金supported by the National Natural Science Foundation of China (No.50604014)the Program for New Century Excellent Talent Project(No.NCET-08-0838)the Research Fund of the State Key Laboratory of Coal Resources and Safe Mining,CUMT (No.09KF11)
文摘In light of the possibility of reignition when unsealing the closed fire zones, the occurrence of reignition was simulated by a home-made experimental device, hydrogen variety rule from combustion to unsealing was analyzed, the three-stage generation model of hydrogen during reignition in fire zone was put forward and the probability that hydrogen was taken as an indicator gas was discussed. The results show that: when fire zone is ready to unseal, which is filled with hydrogen at a high concentration, it is more prone to reignite.
文摘Classical management accounting (MA) Focusing on the facilitating perspective, focuses on decision facilitating and influencing (Demski & Feltham, 1976). MA has to provide information to managers and depending on the problem complexity, they have to solve problems in a dyadic way. A dual process model, the heuristic systematic model (HSM), expands this so-called manager-accountant-dyad and shows different cases of actual human information processing. Managers and accountants either process systematically or heuristically. So far, many concepts have been designed in relation to the normative concept of the economical rational principle. Consequently, recent research only uses systematic information processing, based on the principle of the economic man. In this paper, a decision-behavior oriented approach tries to describe actual decision makers such as managers and accountants and shows new possibilities within MA. Therefore, the potential of heuristic information processing is analyzed, based on the phenomenon of ecological rationality as one shape of bounded rationality. Thus, different cognitive heuristics in business economics are identified and analyzed. Furthermore, the outstanding performance of heuristics compared with more complex calculations is shown. Unfortunately, these findings have been limited to marketing and investments so far. Significant research is needed, regarding conditions for applications and success factors of heuristics in business economics. New empirical findings have to be explicitly transferred to MA.
文摘This article mainly introduces the multi-layer distributed C/S architecture of system design scheme. Its working principle is the client program runs automatically after the computer starts, and establish communication with the application server. The network administrator can monitor and intelligent management of the client computer through the server program, the computer will execute the corresponding operation according to the server to send command instructions. The system realize the main module of the whole system framework, network monitoring data initialization module, network data transmission module, image coding and decoding module, the advantages of system make full use of existing LAN resources, timely delivery and manaRement information.
文摘The scramjet and maglev engineering technology development and trends at home and abroad are firstly presented in this paper. A new launch mode of space transportation system is proposed based on scramjet and magnetic suspension technologies, and its key technologies required are given. This paper also makes analysis on using scramjet and magnetic suspension technologies to launch a reusable rocket, and the results show that a normal temperature conductor maglev launch system is feasible.
基金Supported by the National Natural Science Foundation of China(61273039,21276137)the National Science Fund for Distinguished Young Scholars of China(61525304)
文摘To address large scale industrial processes,a novel Lagrangian scheme is proposed to decompose a refinery scheduling problem with operational transitions in mode switching into a production subproblem and a blending and delivery subproblem.To accelerate the convergence of Lagrange multipliers,some auxiliary constraints are added in the blending and delivery subproblem.A speed-up scheme is presented to increase the efficiency for solving the production subproblem.An initialization scheme of Lagrange multipliers and a heuristic algorithm to find feasible solutions are designed.Computational results on three cases with different lengths of time horizons and different numbers of orders show that the proposed Lagrangian scheme is effective and efficient.
文摘What language competence a learner has can she/he achieve an effective communication? This question is the main concern for applied linguists and English teachers in TEFL, for the recognition of language competence directly influences the goal of teaching, curriculum design, textbook writing and classroom practice. This paper discusses the various points of views on language competence and explores its characteristics which, the author believes, will shed some light on foreign language teaching.
基金National Basic Research Program of China(2007CB209506)
文摘The flowing mechanism of a low permeability gas reservoir is different from a conventional gas reservoir,especially for that with higher irreducible water saturation the threshold pressure gradient exists. At present,in all the deliverability equation,the additional pressure drop caused by the threshold pressure gradient is viewed as constant,but this method has big error in the practical application. Based on the non-Darcy steady flow equation,the limited integral of the additional pressure drop is solved in this paper and it is realized that the additional pressure drop is not a constant but has something to do with production data,and a new deliverability equation is derived,with the relevant processing method for modified isochronal test data. The new deliverability equation turns out to be practical through onsite application.
文摘This paper deals with experimental study of flow field of starting process in two-dimensional, single-stage supersonic ejector on different air total pressure. Schlieren pictures of flow field were taken, static pressure distribu-tions on side wall were measured. The obtained results show that, on critical pressure, the starting main shock waves in ejector oscillated back and forth between the second throat and the middle section of the mixing chamber, it causes the pressure in the second half of the mixing chamber acutely fluctuated .When the working pressure of the active flow is higher than the critical starting pressure, ejector starts normally and the inner flow-field of the mixing chamber keeps stable and the shock waves in the second throat have a certain degree of oscillation . After ejector starts, the operating pressure of the active flow may be lower than the starting pressure .
基金supported by the National Natural Science Foundation of China(Grant No.51375300)the Research Project of State Key Laboratory of Mechanical System and Vibration(Grant No.MSVZD201401)
文摘To reduce the fuel consumption of internal combustion engines, more attention has been paid to the tribological performance of the piston ring pack during the cold start and idle operations. In this research, a numerical model considering the cylinder liner deformation and the piston ring conformability is developed to predict the blow-by, lubrication, friction and wear of the piston ring pack under different operating conditions. The gas flow rate, inter-ring gas pressures, minimum oil film thickness, frictional force and wear load during cold start are calculated and compared with those during warm idle operating conditions. The results show that cylinder liner deformation and piston ring conformability together obviously affect blow-by and other tribological performance. Meanwhile, it is found that friction loss is larger during cold start than during warm idle operating conditions. However, the wear process is more severe during warm idle operation than during cold start. From this research, the blow-by and tribological performance of the piston ring pack during cold start and warm idle operations are understood more deeply.
基金This work was supported by the National Natural Science Foundation of China (31471208, 31671195, and 31271370), the Natural Science Foundation of Hubei Province (2016CFA012), the Fundamental Research Funds for the Central Universities, Integrated Innovative Team for Major Human Diseases of Tongji Medical College, and the Front Youth Program, Huazhong University of Science and Technology.
文摘White adipocytes play important roles in many physiological processes, including energy storage, endocrine signaling, and inflammatory responses. Understanding the molecular mechanisms of adipocyte formation (adipogenesis) provides insights into therapeutic approaches against obesity and its related diseases. Many transcriptional Factors and epigenetic enzymes are known to regulate adipogenesis; however, whether histone variants play a role in this process is unknown. Here we found that macroH2A1.1 (mH2A1.1), a variant of histone H2A, was upregulated during adipocyte differentiation in 3T3-L1 ceils and in the white adipose tissue of obese mice. Ablation of mH2A1.1 activated Wnt/β-catenin signaling pathway, while overexpression of mH2A1.1 showed opposite effects. We farther found that mH2A1.1 regulated Wnt/β-catenin signaling pathway by cooperating with EZH2, a histone H3K27 methyltrans- ferase, thus led to accumulation of H3K27me2 and H3K27me3 on the promoters of Wnt genes. Mutations in the macro-domain, mH2A1.1G224E, and mH2A1.1G314E, not only impaired adipogenesis, but also impaired the binding ability of mH2A1.1 to EZH2 and the enrichments of H3K27me2 and H3K27me3 on the promoters of Wnt genes. Together, our study reveals a novel regulatory role of mH2A1.1 in adipogenesis and obesity, which provides new insights in white fat development.