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长沙汉墓随葬动物形象的“器物观念” 被引量:1
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作者 杨平平 《南方文物》 2013年第2期54-60,共7页
本文探讨了长沙汉墓随葬品不同"动物"形象所蕴含的不同的人文观念,借用美术考古学家的"器物观念"概念,分析不同"动物"形象背后隐含的古人精神世界。笔者将"动物"形象分为现实动物形象、神话&qu... 本文探讨了长沙汉墓随葬品不同"动物"形象所蕴含的不同的人文观念,借用美术考古学家的"器物观念"概念,分析不同"动物"形象背后隐含的古人精神世界。笔者将"动物"形象分为现实动物形象、神话"动物"形象和"动物"组合形象三类,分析其所依次表现出的"事死如事生"的世俗观念、以龙凤为主的神话观念和"天地相协"的理想观念。 展开更多
关键词 “器物观念” “动物”形象 长沙汉墓
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Freestanding oxide membranes:synthesis,tunable physical properties,and functional devices
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作者 Ao Wang Jinfeng Zhang Lingfei Wang 《中国科学技术大学学报》 CAS CSCD 北大核心 2024年第7期2-17,1,I0002,共18页
The study of oxide heteroepitaxy has been hindered by the issues of misfit strain and substrate clamping,which impede both the optimization of performance and the acquisition of a fundamental understanding of oxide sy... The study of oxide heteroepitaxy has been hindered by the issues of misfit strain and substrate clamping,which impede both the optimization of performance and the acquisition of a fundamental understanding of oxide systems.Recently,however,the development of freestanding oxide membranes has provided a plausible solution to these substrate limitations.Single-crystalline functional oxide films can be released from their substrates without incurring significant damage and can subsequently be transferred to any substrate of choice.This paper discusses recent advancements in the fabrication,adjustable physical properties,and various applications of freestanding oxide perovskite films.First,we present the primary strategies employed for the synthesis and transfer of these freestanding perovskite thin films.Second,we explore the main functionalities observed in freestanding perovskite oxide thin films,with special attention to the tunable functionalities and physical properties of these freestanding perovskite membranes under varying strain states.Next,we encapsulate three representative devices based on freestanding oxide films.Overall,this review highlights the potential of freestanding oxide films for the study of novel functionalities and flexible electronics. 展开更多
关键词 freestanding oxide membranes transition metal oxides thin films electronic devices
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《白虎通义》中的道德意识 被引量:2
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作者 向晋卫 《南都学坛(南阳师范学院人文社会科学学报)》 2004年第6期21-25,共5页
作为东汉的一部官方经学著作,《白虎通义》一书的主要内容充满了极其浓厚的道德意识。《白虎通义》全书主要运用了"文字训诂"和"象征"两种手段,对诸如"姓氏"、"爵号"、"谥号"等儒家... 作为东汉的一部官方经学著作,《白虎通义》一书的主要内容充满了极其浓厚的道德意识。《白虎通义》全书主要运用了"文字训诂"和"象征"两种手段,对诸如"姓氏"、"爵号"、"谥号"等儒家的礼制名号和诸如"瑞贽"、"衣裳"、"冠冕"等儒家的礼制器物进行了极为浓重的道德化解释,这样做的结果是一方面从道德上加强了儒家所提倡的"礼乐制度"的合理性;另一方面,具有浓厚道德意蕴的"名号"、"器物"对现实中的社会政治秩序也形成了一种强有力的规范和引导。 展开更多
关键词 《白虎通义》 道德意识 “名号” “器物” 东汉
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BSIM Model Research and Recent Progress 被引量:5
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作者 何进 陈文新 +4 位作者 奚雪梅 宛辉 品书 阿里.力克纪达 胡正明 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2006年第3期388-396,共9页
The continued development of CMOS technology and the emergence of new applications demand continued improvement and enhancement of compact models. This paper outlines the recent work of the BSIM project at the Univers... The continued development of CMOS technology and the emergence of new applications demand continued improvement and enhancement of compact models. This paper outlines the recent work of the BSIM project at the University of California, Berkeley,including BSIM5 research, BSIM4 enhancements, and BSIMSOI development. BSIM5 addresses the needs of nano-CMOS technology and RF high-speed CMOS circuit simulation. BSIM4 is a mature industrial standard MOSFET model with several improvements to meet the technology requirements. BSIMSOI is developed into a generic model framework for PD and FD SOI technology. An operation mode choice,via the calculation of the body potential △Vbi and body current/charge,helps circuit designers in the trend of the coexistence of PD and FD devices. 展开更多
关键词 compact modeling BSIM5 BSIM4 BSIMSOI device physics MOSFETS
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Biological Effects of Stevia rebaudianum Induced by Carbon Ion Implantation 被引量:9
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作者 沈明山 蒋先志 +2 位作者 徐金森 陈亮 陈睦传 《Acta Botanica Sinica》 CSCD 2000年第9期892-897,共6页
The biological effects during seed germination were investigated after the dry seeds of Stevia rebaudianum Bertoni were implanted with carbon ion beam of 75 keV and 10 14 ions/cm 2. The results showed that the g... The biological effects during seed germination were investigated after the dry seeds of Stevia rebaudianum Bertoni were implanted with carbon ion beam of 75 keV and 10 14 ions/cm 2. The results showed that the germination rate of carbon ion implanted seeds was slightly higher than that of the control, but the survival rate of the treated seedlings, on the contrary, was lower than that of the control (P<0.02), while the height of the treated seedlings was significantly higher than that of the control (P<0.01). On the 4th day after germination, the leaf cell wall in the treated group was thick, some high electron_dense substance deposited in the enlarged plasmodesma; Cell membrane creased with high electron_dense granules deposited on it. The plasma membrane protruded towards cell wall, and the granules shifted via plasmodesma or deposited onto cell wall. These phenomena may be related to the conveyance of implanted ions across cell wall, or be related to the accumulation of callose. In addition, the implantation of carbon ions could increase the lamellae of the chloroplast and cause high development of the chloroplast which sometimes contained two plastid centers in an individual chloroplast. Also, the highly developed cristae, abundant mitochondria and typical crystalloid structure in microbody could be found. All these results indicated that the anabolic and catabolic activities in the seedlings implanted with carbon ions before germination were obviously more active than those in the controls. 展开更多
关键词 Stevia rebaudianum carbon ion implantation ORGANELLE biology effects
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Leaching of nickel-molybdenum sulfide ore in membrane biological reactor 被引量:1
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作者 陈家武 高从堦 +2 位作者 张启修 肖连生 张贵清 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2011年第6期1395-1401,共7页
The bioleaching of molybdenum from its sulfide ore using a Mo-resistant thermophilic bacterium sulfolobus metallics combined with a membrane biological reactor(MBR) was studied.The experimental results showed that t... The bioleaching of molybdenum from its sulfide ore using a Mo-resistant thermophilic bacterium sulfolobus metallics combined with a membrane biological reactor(MBR) was studied.The experimental results showed that the concentration of Mo can be controlled by filter of the membrane in MBR and the toxicity of Mo to microorganism is decreased in the process of bioleaching.It was also evidenced that there were different leaching rates of Ni and Mo when the concentration of Mo was different.After leaching for 20 d in the MBR at Mo concentration of 395 mg/L,the leaching rates of Ni and Mo reached the maximum of 79.57% and 56.23% respectively under the conditions of 100 g/L of mineral density,65 ℃,pH=2 and 1.0 L/min of the aeration rate.While 75.59% Ni and 54.33% Mo were leached out in column without membrane under the same conditions. 展开更多
关键词 membrance biological reactor nickel-molybdenum sulfide ore sulfolobus metallics BIOLEACHING molybdenum toxicity
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Establishment of a Collection and Detection Technology of Mycoplasma hyopneumoniae in Aerosol 被引量:1
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作者 华利忠 高昆 +4 位作者 武昱孜 白方方 冯志新 刘茂军 邵国青 《Agricultural Science & Technology》 CAS 2013年第7期998-1001,1031,共5页
ObjectiveThis study was to establish a simple method for collecting and detecting Mycoplasma hyopneumoniae (Mhp) in aerosol. MethodBased on the mechanisms of liquid impinger and filtration sampler, a double concentr... ObjectiveThis study was to establish a simple method for collecting and detecting Mycoplasma hyopneumoniae (Mhp) in aerosol. MethodBased on the mechanisms of liquid impinger and filtration sampler, a double concentration aerosol sampler was designed for collecting Mhp aerosol. Firstly, the collection was performed in a closed environment full of artificial aerosol of Mhp. Secondly, collection efficiency was detected by real-time PCR. Thereafter, the clinical feasibility of the designed equipment was tested by collecting aerosol samples in different pig herds. In one assay, the samples were collected at different times from one pig house challenged with Mhp. In another assay, the samples was collected from the delivery room, nursery and fattenning house of a MPS outbreak farm as well as a Mhp infection positive pig farm without obvious clinical symptoms. All the aerosol samples were then detected by real-time PCR or nested PCR. ResultThe collection efficiency of the designed bioaerosol sampler was (37.04±6.43) %, Mhp could be detected 7 d after intratracheal challenge with pneumonic lung homogenate suspension. Aerosol samples of 11 pig houses from the two Mhp positive pig farms with or without clinical symptoms all showed a positive result of PCR, the positivity rate was 100%. ConclusionA high sensitive collecting and detecting technology of aerosol was successfully established, which can be applied to clinical detection of Mhp in aerosol. 展开更多
关键词 Mycoplasma hyopneumoniae AEROSOL Bioaerosol sampler DETECTION
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Physical layer design of wireless sensor network nodes 被引量:5
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作者 钟子果 胡爱群 王丹 《Journal of Southeast University(English Edition)》 EI CAS 2006年第1期21-25,共5页
Major consideration dimensions for the physical layer design of wireless sensor network (WSN) nodes is analyzed by comparing different wireless communication approaches, diverse mature standards, important radio fre... Major consideration dimensions for the physical layer design of wireless sensor network (WSN) nodes is analyzed by comparing different wireless communication approaches, diverse mature standards, important radio frequency (RF) parameters and various microcontroller unit (MCU) solutions. An implementation of the WSN node is presented with experimental results and a novel "one processor working at two frequencies" energy saving strategy. The lifetime estimation issue is analyzed with consideration to the periodical listen required by common WSN media access control (MAC) algorithms. It can be concluded that the startup time of the RF which determines the best sleep time ratio and the shortest backoff slot time of MAC, the RF frequency and modulation methods which determinate the RX and TX current, and the overall energy consumption of the dual frequency MCU SOC ( system on chip) are the most essential factors for the WSN node physical layer design. 展开更多
关键词 wireless sensor network node physical layer radio frequency energy consumption node lifetime
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Effect of Flating Photobioreactor Light-path on the Growth and Organic Matter Accumulation of Isochrysis galbana Parke
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作者 吴电云 邹宁 +1 位作者 高维锡 黄清荣 《Agricultural Science & Technology》 CAS 2010年第11期7-9,共3页
[Objective] The paper aims to provide references to cultivate I.galbana in a large scale and explore its medical value.[Method] I.galbana had been cultivated in different light-path flat plate bioreactors,and the grow... [Objective] The paper aims to provide references to cultivate I.galbana in a large scale and explore its medical value.[Method] I.galbana had been cultivated in different light-path flat plate bioreactors,and the growth condition and content of organic matter within cells of I.galbana had been analyzed.[Result] Growth rate and volumetric productivity of I.galbana cells increased as light-path of flat plate bioreactors decreased,however,daily areal output rate rose with the increasing of light path.The smaller the light path of flat plate bioreactors was,the more content of total lipids,protein and polysaccharide it had in I.galbana cells.[Conclusion] It is an effective way to improve production efficiently and reduce the cost by selecting the suitable bioreactors. 展开更多
关键词 Isochrysis galbana parke Light-path PHOTOBIOREACTOR PRODUCTIVITY Organic matter
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The BLOC Interactomes Form a Network in Endosomal Transport
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作者 李巍 冯雅琴 +4 位作者 郝婵娟 郭小黎 崔艳艳 贺敏 何新 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第8期669-682,共14页
With the identification of more than a dozen novel Hermansky-Pudlak Syndrome (HPS) proteins in vesicle trafficking in higher eukaryotes, a new class of trafficking pathways has been described. It mainly consists of ... With the identification of more than a dozen novel Hermansky-Pudlak Syndrome (HPS) proteins in vesicle trafficking in higher eukaryotes, a new class of trafficking pathways has been described. It mainly consists of three newly-defined protein com- plexes, BLOC-l, -2, and -3. Compelling evidence indicates that these complexes together with two other well-known complexes, AP3 and HOPS, play important roles in endosomal transport. The interactions between these complexes form a network in protein trafficking via endosomes and cytoskeleton. Each node of this network has intra-complex and extra-complex interactions. These complexes are connected by direct interactions between the subunits from different complexes or by indirect interactions through coupling nodes that interact with two or more subunits from different complexes. The dissection of this network facilitates the understanding of a dynamic but elaborate transport machinery in protein/membrane trafficking. The disruption of this network may lead to abnormal trafficking or defective organellar development as described in patients with Hermansky-Pudlak syndrome. 展开更多
关键词 biogenesis of lysosome-related organelles complex (BLOC) endosomal transport protein interactome Hermansky-Pudlak
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Study on the Flowering Biological Characteristics of Prunus domestica × armeniaca 被引量:1
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作者 魏雅君 李雯雯 +5 位作者 冯贝贝 徐业勇 王明 杨红丽 牛莹莹 廖康 《Agricultural Science & Technology》 CAS 2016年第3期577-583,共7页
[Objective] The aims of this study were to understand the flowering biological characteristics of Prunus domestica ×armeniaca and analyze its differences with other apricot varieties and plum varieties. [Method] ... [Objective] The aims of this study were to understand the flowering biological characteristics of Prunus domestica ×armeniaca and analyze its differences with other apricot varieties and plum varieties. [Method] Taking three varieties of Prunus domestica ×armeniaca as the research object, the phenophase of blooming,morphology characteristics of floral organs and development characteristics of floral organs were observed, pollen amount, pollen viability and stigma receptivity were measured. [Result] The results showed that three varieties of Prunus domestica×armeniaca were in blossom in early April, the florescence were relatively late for 8-12 d comparing with the varieties of apricot, the florescence were relatively similar to the P. domestica L. Morphology characteristics of floral organs of three varieties of Prunus domestica×armeniaca had few differences, were relatively similar to two varieties of plum, and they all had high percent of middle-style and long-style flower.The pollen amount of three varieties of Prunus domestica ×armeniaca had a little differences comparing with two varieties of plum, but were lower than three varieties of apricot. There were some differences in pollen viability, the germination percentage of pollen of ‘Konglongdan' was relatively high, was 51.6%; The pollen amount of ‘Fengweihuanghou' was relatively high, was 880.8 per anther; ‘Weidi' had fewer pollen amount, and the pollen viability was just 2.1%; Three varieties of Prunus domestica ×armeniaca all had strong stigma receptivity on the bloom day, and the stigma receptivity continued exist until the day that 5 days after blooming. [Conclusion] The florescence of three varieties of Prunus domestica ×armeniaca were relatively concentrated, and the rate of pistil abortion was lowest, the variation intensity of stigma receptivity and sustainable time of three varieties of Prunus domestica×armeniaca almost had a little differences comparing with a variety of plum, but slightly more than three varieties of apricot. 展开更多
关键词 Prunus domestica×armeniaca Blooming phenophase Floral organs Pollen viability Stigma receptivity
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STUDY AND SIMULATION OF AN ACTIVATED SLUDGE TREATMENT FOR METHYL VIOLET WASTE WATER
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作者 张金利 宋宽秀 +1 位作者 王一平 闻建平 《Transactions of Tianjin University》 EI CAS 1998年第2期72-75,共4页
This paper researched a promising biological treatment of methyl violet waste water by methods of activated sludge.Effects of temperature and pH were studied on this process.Kinetic equation ... This paper researched a promising biological treatment of methyl violet waste water by methods of activated sludge.Effects of temperature and pH were studied on this process.Kinetic equation of the substrate biodegradation was investigated in the experimental range.It was studied and simulated that flow within the bubble region of this bioreactor according to the κ ε two fluid equation.Simulation results agree well with experimental data. 展开更多
关键词 bioreactor waste water treatment activated sludge
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A Discovery of Anomozamites with Reproductive Organs 被引量:2
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作者 郑少林 张立军 巩恩普 《Acta Botanica Sinica》 CSCD 2003年第6期667-672,共6页
Anomozamites haifanggouensis (Kimura et al.) Zheng et Zhang comb. nov. is a characteristic Bennettitalean plant in Middle Jurassic flora. Only one specimen was collected from Haifanggou Formation (Middle Jurassic) in ... Anomozamites haifanggouensis (Kimura et al.) Zheng et Zhang comb. nov. is a characteristic Bennettitalean plant in Middle Jurassic flora. Only one specimen was collected from Haifanggou Formation (Middle Jurassic) in Daohugou village, Shantou town, Ningcheng County, Nei Mongol Autonomous Region, China. The stem of plant is slender and repeatedly branches as a dichasial system with a fertile shoot in the fork, forming equal and widely divergent branches, on the branches born with the fronds of Anomozamites type. Its reproductive organs consists of some microsporophylls and a few of the bracteoid small leaves (or receptacular leaves). Some scattered bracteoid leaves and microsporophylls were formerly considered by Pan as dicots. Since then, they were proposed to be a non-committal genus Pankuangia and described them as P. haifanggouensis by Kimura et al. In this study, we discover that these bracteoid small leaves and microsporophylls should be of Anomozamites. This discovery provides strong evidence for the 'Pankuangia' determination of the taxonomical position. 展开更多
关键词 Anomozamites haifanggouensis (Kimura et al.) comb. nov. reproductive organ Nei Mongol Middle Jurassic Haifanggou Formation
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Chloroplast Genetic Engineering in Higher Plants 被引量:1
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作者 张景昱 张嫄 宋艳茹 《Acta Botanica Sinica》 CSCD 2003年第5期509-516,共8页
Chloroplast genetic engineering, with several advantages over nuclear genetic engineering, is now regarded as an attractive new technology in basic and applied research, including deepening our understanding of plasti... Chloroplast genetic engineering, with several advantages over nuclear genetic engineering, is now regarded as an attractive new technology in basic and applied research, including deepening our understanding of plastid genome, engineering plant metabolic system, generating transplastomic plants with higher resistance to insect, disease, drought and herbicide and bioproducing of antibodies and vaccines. In this review, the principle and operating system for chloroplast genetic engineering and its application in higher plants have been discussed. 展开更多
关键词 chloroplast genetic engineering transplastomic plants plastid genome
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A High-Performance Silicon Electro-Optic Phase Modulator with a Triple MOS Capacitor 被引量:2
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作者 黄北举 陈弘达 +2 位作者 刘金彬 顾明 刘海军 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2006年第12期2089-2093,共5页
We propose and analyze a novel Si-based electro-optic modulator with an improved metal-oxide-semiconductor (MOS) capacitor configuration integrated into silicon-on-insulator (SOl). Three gate-oxide layers embedded... We propose and analyze a novel Si-based electro-optic modulator with an improved metal-oxide-semiconductor (MOS) capacitor configuration integrated into silicon-on-insulator (SOl). Three gate-oxide layers embedded in the silicon waveguide constitute a triple MOS capacitor structure, which boosts the modulation efficiency compared with a single MOS capacitor. The simulation results demonstrate that the Vπ Lπ product is 2. 4V · cm. The rise time and fall time of the proposed device are calculated to be 80 and 40ps from the transient response curve, respectively,indicating a bandwidth of 8GHz. The phase shift efficiency and bandwidth can be enhanced by rib width scaling. 展开更多
关键词 carrier accumulation plasma dispersion effect electro-optic phase modulator METAL-OXIDE-SEMICONDUCTOR optoelectronic integrated circuit
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Electron Injection Enhancement by Diamond-Like Carbon Film in Polymer Electroluminescence Devices
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作者 李宏建 闫玲玲 +4 位作者 黄伯云 易丹青 胡锦 何英旋 彭景翠 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2006年第1期30-34,共5页
A diamond-like carbon (DLC) film is deposited as an electron injection layer between the polymer light-emitting layer(MEH-PPV) and aluminum (Al) cathode electrode in polymer electroluminescence devices (PLEDs)... A diamond-like carbon (DLC) film is deposited as an electron injection layer between the polymer light-emitting layer(MEH-PPV) and aluminum (Al) cathode electrode in polymer electroluminescence devices (PLEDs) using a radio frequency plasma deposition system. The source material of the DLC is n-butylamine. The devices consist of indium tin oxide (ITO)/MEH-PPV/DLC/Al. Electron injection properties are investigated through I-V characteristics,and the mechanism of electron injection enhancement due to a thin DLC layer has been studied. It is found that: (1) a DLC layer thinner than 1.0nm leads to a higher turn-on voltage and decreased electroluminescent (EL) efficiency; (2) a 5.0nm DLC layer significantly enhances the electron injection and results in the lowest turn-on voltage and the highest EL efficiency; (3) DLC layer that exceeds 5.0nm results in poor device performance;and(4) EL emission can hardly be detected when the layer exceeds 10.0nm. The properties of ITO/MEH-PPV/DLC/Al and ITO/MEH-PPV/LiF/Al are investigated comparatively. 展开更多
关键词 diamond-like carbon polymer electroluminescence device electron injection enhancement
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The Flowering Biology of Plum(Prunus domestica L.) in Aksu Region, Xinjiang
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作者 冯贝贝 李雯雯 +5 位作者 魏雅君 徐业勇 王明 杨红丽 牛莹莹 廖康 《Agricultural Science & Technology》 CAS 2016年第6期1371-1375,共5页
[Objective] The aim of this study was to understand the flowering biological characteristics of different varieties Prunus domestica L. in Aksu Jiamu Experi- ment Station. [Method] Three varieties of plum as Prunus do... [Objective] The aim of this study was to understand the flowering biological characteristics of different varieties Prunus domestica L. in Aksu Jiamu Experi- ment Station. [Method] Three varieties of plum as Prunus domestica L. cv. 'French', Prunus domestica L. cv. "Victoria' and Prunus domestica L. cv. "Stanley' were the research subjects. The phenophases of blooming, and the morphological and developmental characteristics of floral organs were observed. The amount and viability of pollen and the stigma receptivity were measured. [Result] Three varieties of plum all blossomed in early April, and the florescence had lasted for 9-10 d. The average number of anthers was 28.48%. The average number of pollen grains in each flower was 28 700.0. The pollen germination rate was 27.4%. 86.75% of the flowers were medium-styled or long-styled. There was no significant difference in the petal shape, color, anther number, calyx color, and flower ovary among the three varieties of plum. The stigma receptivity was strongest on the first day of flowering, and then dropped gradually. Therefore, the flowers of plum were manual pollinated on the first day of flowering. The duration time of stigma receptivity of Prunus domestica L. cv. 'Victoria' was longer than the other cultivars, the duration time of stigma receptivity was about 5 d. [Conclusion] The pollen number and pollen germination rate of Prunus domestica L. cv. 'Stanley' were higher than the other cultivars, which be considered the most suitable pollinizer for plum. In contrast, the complete flower rate of Prunus domestica L. cv. 'French' and Prunus domestica L. cv. 'Victoria' was relatively high, but the pollen number and pollen germination rate of Prunus domestica L. cv. 'French' and Prunus domestica L. cv. 'Victoria' were lower than Prunus domestica L. cv. 'Stanley', which are not suitable to use as a pollinizer for plum. 展开更多
关键词 Prunus domestica L. PHENOPHASE Floral organs Pollen viability Stigma receptivity
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RESEARCH ON MEMBRANE BIOREACTOR TREATING DOMESTIC WASTEWATER AND RELATED MECHANISMS
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作者 顾平 周丹 +1 位作者 杨造燕 刘锦霞 《Transactions of Tianjin University》 EI CAS 2001年第1期7-11,共5页
Experiments on treatment of domestic wastewater by membrane bioreactors were carried out.The results showed that this process could produce good quality effluent with low COD,turbidity and total count of bacteria.With... Experiments on treatment of domestic wastewater by membrane bioreactors were carried out.The results showed that this process could produce good quality effluent with low COD,turbidity and total count of bacteria.With intermittent operation and continuous aeration,the membrane flux was kept steady.The mechanisms of removing COD through membrane,the structure of membrane and filtration resistance were also discussed. 展开更多
关键词 wastewater treatment domestic wastewater membrane bioreactor electronic microscopic photograph filtration resistance
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Viable but Non-culturable Bacteria in Bioreactorbased Pharmaceutical Wastewater
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作者 邹艳艳 付惠玲 +4 位作者 陈雅红 翟竟余 韦彦斐 梅荣武 丁林贤 《Agricultural Science & Technology》 CAS 2014年第8期1299-1303,共5页
We aimed to investigate the composition and phylogenetic rela-tionships of the viable but non-culturable (VBNC) state bacteria in pharmaceutical wastewater. [Method] Soil filter was used for constructing bioreactor.... We aimed to investigate the composition and phylogenetic rela-tionships of the viable but non-culturable (VBNC) state bacteria in pharmaceutical wastewater. [Method] Soil filter was used for constructing bioreactor. Based on the resuscitation- and growth-promoting function of Resuscitation Promoting Factor (Rpf) for VBNC bacteria, VBNC bacteria were isolated by most probable number (MPN) method and dilution-plating method and 16S rRNA gene phylogenetic analysis was carried out. [Result] In MPN culture system, Rpf could promote the resuscitation and growth of some bacteria. There were VBNC advantage floras that sensitive to Rpf in pharmaceutical wastewater. The culturable VBNC bacteria in pharmaceutical wastewater consisted of high-GC gram-positive actinomycetes including genera Mi-crobacterium, Gordonia and Leucobacter, and gram-negative bacteria including gen-era Candidimonas, Xanthobacter and Aminobacter. Four strains (ZYM1, ZYM3, ZYZR4, ZYXR1) could be potential novel species. [Conclusion] This research re-vealed there were VBNC bacteria in pharmaceutical wastewater. These results could provide important ideas and methods for further studies on VBNC bacteria in the pharmaceutical wastewater, especial y the formation mechanism and recovery mech-anism of VBNC bacteria and the advanced degradation process improvement of pharmaceutical wastewater. 展开更多
关键词 Pharmaceutical wastewater BIOREACTOR VBNC bacteria Rpf 16S rRNAgene phylogenetic relationship
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Mushroom Pulp Tissue-Based Membrane-Ferrocene-Modified L-Tyrosine Biosensor
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作者 马全红 邓家祺 《Journal of Southeast University(English Edition)》 EI CAS 2000年第1期106-110,共5页
A new approach for assembling amperometric mushroom pulp tissue based membrane electrode for determination of L tyrosine analysis is proposed. Ferrocene is used as a mediator of electron transfer between tyrosinase ... A new approach for assembling amperometric mushroom pulp tissue based membrane electrode for determination of L tyrosine analysis is proposed. Ferrocene is used as a mediator of electron transfer between tyrosinase in mushroom tissue and a graphite electrode. The optimal operation conditions are studied. The linear response range of the biosensor is 2 0×10 -4 to 4 5×10 -3 mol·L -1 with response time of less than 5 min and lifetime of at least 30 d. The biosensor can be applied to practical sample analysis. 展开更多
关键词 BIOSENSOR tissue based membrane electrode modified electrode FERROCENE L tyrosine
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