With reference to the recent achivements about the structure, spectra and kinetics of light_harvesting complex (LHCⅡ) in PSⅡ of higher plants, a four_level model was provided to simulate the energy transfer process ...With reference to the recent achivements about the structure, spectra and kinetics of light_harvesting complex (LHCⅡ) in PSⅡ of higher plants, a four_level model was provided to simulate the energy transfer process from LHC Ⅱ to the reaction center. On the basis of this model, a set of rate equation was established. Analysis of its algebra solution led to a general picture of energy transfer process in LHC Ⅱ of higher plants and the strong interaction among pigment molecules in this process. Based on the spectra, kinetics and biological structural data providing some information of energy transfer path and energy dissipation mechanism, it has been found that energy transfer mainly happened between the pigments whose energy level was most closely adjacent, the loss of energy had a close relation to the process of energy transfer and tended to increase with the decrease of energy level. The protective mechanism of antenna system was also discussed.展开更多
In nature, quadrupeds as horses or cats change their gait to be suited to their motion speed. This fact is very important to realize smooth motion with different gaits. In this article a method of the gait transition ...In nature, quadrupeds as horses or cats change their gait to be suited to their motion speed. This fact is very important to realize smooth motion with different gaits. In this article a method of the gait transition for the quadruped walking machine is proposed. With analyzing the accustomed gaits of quadruped, some standard gaits and the relationships between duty factor and the phasic differences of the legs corresponding to the first leg could be concluded. With this, the gaits transferring could be continuous. Besides, celerity and on line method for generation and modifying gaits is studied, which can make the robot have a smooth motion to adapt the uncharted rough terrain.展开更多
The structures of human telomeric DNA have received much attention due to its significant biological importance.Most studies have focused on G-quadruplex structure formed by short telomeric DNA sequence,but little is ...The structures of human telomeric DNA have received much attention due to its significant biological importance.Most studies have focused on G-quadruplex structure formed by short telomeric DNA sequence,but little is known about the structures of long singlestranded telomeric DNAs.Here,we investigated the structure of DNA with a long sequence of d[AGGG(TTAGGG)6](G6-DNA)and the effect of a single repeat sequence d(TTAGGG)(G01-DNA)on the structure of G6-DNA using sedimentation velocity technique,polyacrylamide gel electrophoresis,circular dichroism spectroscopy,and UV melting experiments.The results suggest that the G6-DNA can form dimers in aqueous solutions and G01-DNA can form additional G-quadruplex structures by binding to G6-DNA.However,G01-DNA has no effect on the structure of DNA with a sequence of d[AGGG(TTAGGG)3](G3-DNA).Our study provides new insights into the structure polymorphism of long human single-stranded telomeric DNA.展开更多
Purple soils are widely distributed in the Sichuan Hilly Basin and are highly susceptible to erosion, especially on the cultivated slopes. Quantitative assessment of the erosion rates is, however, difficult due to sma...Purple soils are widely distributed in the Sichuan Hilly Basin and are highly susceptible to erosion, especially on the cultivated slopes. Quantitative assessment of the erosion rates is, however, difficult due to small size of the plots of the manually-tilled land, the complex land use, and steep hillslopes. ^137Cs and ^210Pbex (excess ^210Pb) tracing techniques were used to investigate the spatial pattern of soil erosion rates associated with slope-land under hoe tillage in Neijiang of the Sichuan Hilly Basin. The ^137Cs and ^210Pbex inventories at the top of the cultivated slope were extremely low, and the highest inventories were found at the bottom of the cultivated slope. By combining the erosion rates estimates provided by both ^137Cs and 21~Pbex measurements, the weighted mean net soil loss from the study slope was estimated to be 3 100 t km^-2 year^-1, which was significantly less than 6 930 t km^-2 year^-1 reported for runoff plots on a 10° cultivated slope at the Suining Station of Soil Erosion. The spatial pattern of soil erosion rates on the steep agricultural land showed that hoe tillage played an important role in soil redistribution along the slope. Also, traditional farming practices had a significant role in reducing soil loss, leading to a lower net erosion rate for the field.展开更多
The problem of joint direction of arrival(DOA)and polarization estimation for polarization sensitive coprime planar arrays(PS-CPAs)is investigated,and a fast-convergence quadrilinear decomposition approach is proposed...The problem of joint direction of arrival(DOA)and polarization estimation for polarization sensitive coprime planar arrays(PS-CPAs)is investigated,and a fast-convergence quadrilinear decomposition approach is proposed.Specifically,we first decompose the PS-CPA into two sparse polarization sensitive uniform planar subarrays and employ propagator method(PM)to construct the initial steering matrices separately.Then we arrange the received signals into two quadrilinear models so that the potential DOA and polarization estimates can be attained via quadrilinear alternating least square(QALS).Subsequently,we distinguish the true DOA estimates from the approximate intersecting estimations of the two subarrays in view of the coprime feature.Finally,the polarization estimates paired with DOA can be obtained.In contrast to the conventional QALS algorithm,the proposed approach can remarkably reduce the computational complexity without degrading the estimation performance.Simulations demonstrate the superiority of the proposed fast-convergence approach for PS-CPAs.展开更多
The results from interpretation of the aerophotos and in-situ seismogeological researches show that there are some obvious late-Quaternary activities along the Moxi-Mianning segment of the Xianshuihe-Anninghe fault zo...The results from interpretation of the aerophotos and in-situ seismogeological researches show that there are some obvious late-Quaternary activities along the Moxi-Mianning segment of the Xianshuihe-Anninghe fault zone, with the characteristics of sinistral-slip movement accompanied by some significant vertical slip components. Since late-Quaternary, the average horizontal slip rate of the segment at the south of Moxi along the Xianshuihe fault is 6.0~9.9mm/a and 4.7~5.3mm/a along the segment at the north of Mianning of the Anninghe fault. The results from the investigation of coseismic dislocation and ground rupture show that the ground rupture caused by 1876 Kangding-Luding earthquake with M 7 3/ 4 can extend to the south of Tianwan. The segment at the north of Mianning of the Anninghe fault has a background for producing M7.5 earthquake and the geological record of the last strong earthquake must be the proofs of the 1327 earthquake with M>6.0 with poor historical records.展开更多
Microparticle formation and crystallization rate of 1,3,5,7-tetranitro-l,3,5,7-tetraazacyclooctane (HMX) in acetone solution using supercritical carbon dioxide antisolvent (GAS) recrystallization were studied. Scannin...Microparticle formation and crystallization rate of 1,3,5,7-tetranitro-l,3,5,7-tetraazacyclooctane (HMX) in acetone solution using supercritical carbon dioxide antisolvent (GAS) recrystallization were studied. Scanning electronic microscopy, X-ray diffraction and infrared radiation were used to examine particle size, crystallinity and chemical structure. The results show that B-HMX microparticle in different average size (2-9.5um) and with narrow size distribution were obtained by controlling the expansibility, expansion speed, initial concentration and temperature during recrystallization of HMX. The formation of nuclei may be a main cause of consumption of solute when the solution is expanded rapidly enough and the equilibrium concentration is lower, in which almost monodisperse microparticle can be obtained.展开更多
Nonexchangeable K (NEK) is the major portion of the reserve of available K in soil and a primary factor in determining soil K fertility. The questions of how much NEK is in soils and how to quantify total NEK in soi...Nonexchangeable K (NEK) is the major portion of the reserve of available K in soil and a primary factor in determining soil K fertility. The questions of how much NEK is in soils and how to quantify total NEK in soils are so far still unclear due to the complicated effects of various minerals on K fixation. In this study, the NEK in 9 soils was extracted with sodium tetraphenylboron (NaBPh4) for various time periods longer than 1 d. The results showed that the NEK extracted by NaBPh4 gradually increased with time, but showed no more increase after the duration of extraction exceeded 10 20 d. As the temperature increased from 25 to 45 ~C, the duration to obtain the maximum extraction of NEK was reduced from 20 to 10 d, and the maximum values of NEK released at both temperatures was almost the same for each soil. The maximum NEK (MNEK) of the 9 soils extracted by NaBPh4 varied from 3074 to 10081 mg kg-1, accounting for 21% 56% of the total soil K. There was no significant correlation between MNEK released by NaBPh4 and other forms of K, such as NH4OAc-extracted K, HNO3-extracted K and total K in soils, which indicates that NEK is a special form of K that has no inevitable relationship to the other forms of K in soils. The MNEK extraction by NaBPh4 in this study indicated that the total NEK in the soils could be differentiated from soil structural K and quantified with the modified NaBPh4 method. The high MNEK in soils made NEK much more important in the role of the plant-available K pool. How to fraetionate NEK into different fractions and establish the methods to quantify each NEK fraction according to their bioavailability is of great importance for future research.展开更多
The Wenchuan earthquake occurred near the "triple junction" linking the Bayan Har block, the South China block, and the Sichuan-Yunnan rhombic block, and its influences on the surrounding blocks and the main...The Wenchuan earthquake occurred near the "triple junction" linking the Bayan Har block, the South China block, and the Sichuan-Yunnan rhombic block, and its influences on the surrounding blocks and the main fault zones in the Sichuan-Yunnan region, i.e., the block boundary zone, cannot be ignored. In this paper, changes of movement and stress of the fault zones before and after a strong earthquake were simulated based on the GPS repetition survey results recently obtained during 1999–2007, 2009–2011, and 2011–2013 with a two-dimensional finite-element contact model and the "block- loading" method. The results show that, before the Wenchuan earthquake, the movement of the Longmenshan fault zone was very slow and its compressive stress accumulated rapidly; after the Wenchuan earthquake, movements toward the E-SSE direction of the Bayan Har, southwestern Yunnan, and rhombic blocks were enhanced, and the dextral and horizontal compressive speeds and annual accumulative compressive stress of the Longmenshan fault zone increased markedly by factors of 4.5, 2.1, and 2.5, respectively. The southern Xianshuihe, Anninghe, Zemuhe, Daliangshan, and Lijiang-Xiaojinhe fault zones accumulated compressive stress rapidly, forming enhanced compressive stress zones along a NE strike crossing the central part of the Sichuan-Yunnan region. The tensional movement of the Xianshuihe fault zone was enhanced and the slip movement in the central part of the zone was reversed in a short time. The changes are tightly related to the medium-intensity earthquakes that occurred during the same period in this region, revealing that the spatial migration of seismic activity is related to changes of movement of the blocks.展开更多
A crucial part of proposed earthquake early warning systems is a rapid estimate for earthquake magnitude.Most of these methods are focused on the first part of the P-wave train,the earlier and less destructive part of...A crucial part of proposed earthquake early warning systems is a rapid estimate for earthquake magnitude.Most of these methods are focused on the first part of the P-wave train,the earlier and less destructive part of the ground motion that follows an earthquake.A method has been proposed by using the period of the P-wave to determine the magnitude of a large earthquake at local distance,and a specific relation for the Sichuan region was calibrated according to acceleration records of Wenchuan earthquake.The Mw 6.6 earthquake hit Lushan County,Sichuan,on April 20,2013 and the largest aftershocks provide a useful dataset to validate the proposed relation and discuss the risks connected to the extrapolation of magnitude relations with a poor dataset of large earthquake waveforms.A discrepancy between the local magnitude(ML)estimated by means ofτc evaluation and the standard ML(6.4 vs.7.0)suggests using caution when ML vs.τc calibrations do not include a relevant dataset of large earthquakes.Effects from large residuals could be mitigated or removed by introducing selection rules onτc function,by regionalizing the ML vs.τc function in the presence of significant tectonic or geological heterogeneity,and by using probabilistic and evolutionary methods.展开更多
Traditional methods for detecting lactoperoxidase (LP) are complex and time-consuming, so a test strip was made based on the enzymatic reaction principle to enable quick and convenient detection of LP in raw milk. I...Traditional methods for detecting lactoperoxidase (LP) are complex and time-consuming, so a test strip was made based on the enzymatic reaction principle to enable quick and convenient detection of LP in raw milk. In this study 0.1 mol/L citric acid (CA)/0.2 mol/L disodium hydrogen phosphate (NAP) buffer solution (pH 5.0), 22 mmol/L 3,3',5,5'-tetramethylbenzidine (TMB), 0.6 mmol/L hydrogen peroxide (H202), and 0.5% Tween-20 or 0.3% cetyltri- methyl ammonium bromide (CTAB) were optimal for preparing a quick, sensitive, and accurate LP test strip. The coefficient of variation (CV) of the estimated LP concentrations ranged from 2.47% to 6.72% and the minimum LP concentration detected by the test strip was 1-2 mg/L. Estimates of active LP in sixteen raw milk samples obtained using the test strip or the TMB method showed a good correlation (c=0.9776). So the test strip provides a quick, convenient, and accurate method for detecting the LP concentration of raw milk.展开更多
In this paper,we calculated the spatial local-averaged velocity strains along the streamwise direction at four spatial scales according to the concept of spatial local-averaged velocity structure function by using the...In this paper,we calculated the spatial local-averaged velocity strains along the streamwise direction at four spatial scales according to the concept of spatial local-averaged velocity structure function by using the three-dimensional three-component database of time series of velocity vector field in the turbulent boundary layer measured by tomographic time-resolved particle image velocimetry.An improved quadrant splitting method,based on the spatial local-averaged velocity strains together with a new conditional sampling phase average technique,was introduced as a criterion to detect the coherent structure topology.Furthermore,we used them to detect and extract the spatial topologies of fluctuating velocity and fluctuating vorticity whose center is a strong second-quadrant event(Q2) or a fourth-quadrant event(Q4).Results illustrate that a closer similarity of the multi-scale coherent structures is present in the wall-normal direction,compared to the one in the other two directions.The relationship among such topological coherent structures and Reynolds stress bursting events,as well as the fluctuating vorticity was discussed.When other burst events are surveyed(the first-quadrant event Q1 and the third-quadrant event Q3),a fascinating bursting period circularly occurs:Q4-S-Q2-Q3-Q2-Q1-Q4-S-Q2-Q3-Q2-Q1 in the center of such topological structures along the streamwise direction.In addition,the probability of the Q2 bursting event occurrence is slightly higher than that of the Q4 event occurrence.The spatial instable singularity that almost simultaneously appears together with typical Q2 or Q4 events has been observed,which is the main character of the mutual induction mechanism and vortex auto-generation mechanism explaining how the turbulence is produced and maintained.展开更多
文摘With reference to the recent achivements about the structure, spectra and kinetics of light_harvesting complex (LHCⅡ) in PSⅡ of higher plants, a four_level model was provided to simulate the energy transfer process from LHC Ⅱ to the reaction center. On the basis of this model, a set of rate equation was established. Analysis of its algebra solution led to a general picture of energy transfer process in LHC Ⅱ of higher plants and the strong interaction among pigment molecules in this process. Based on the spectra, kinetics and biological structural data providing some information of energy transfer path and energy dissipation mechanism, it has been found that energy transfer mainly happened between the pigments whose energy level was most closely adjacent, the loss of energy had a close relation to the process of energy transfer and tended to increase with the decrease of energy level. The protective mechanism of antenna system was also discussed.
基金Supported by the china postdoctoral Science Foundation (No. 20080430362).
文摘In nature, quadrupeds as horses or cats change their gait to be suited to their motion speed. This fact is very important to realize smooth motion with different gaits. In this article a method of the gait transition for the quadruped walking machine is proposed. With analyzing the accustomed gaits of quadruped, some standard gaits and the relationships between duty factor and the phasic differences of the legs corresponding to the first leg could be concluded. With this, the gaits transferring could be continuous. Besides, celerity and on line method for generation and modifying gaits is studied, which can make the robot have a smooth motion to adapt the uncharted rough terrain.
基金supported by the National Natural Scientific Foundation of China (No.21674107)the Fundamental Research Funds for the Central Universities (No.WK2340000066)
文摘The structures of human telomeric DNA have received much attention due to its significant biological importance.Most studies have focused on G-quadruplex structure formed by short telomeric DNA sequence,but little is known about the structures of long singlestranded telomeric DNAs.Here,we investigated the structure of DNA with a long sequence of d[AGGG(TTAGGG)6](G6-DNA)and the effect of a single repeat sequence d(TTAGGG)(G01-DNA)on the structure of G6-DNA using sedimentation velocity technique,polyacrylamide gel electrophoresis,circular dichroism spectroscopy,and UV melting experiments.The results suggest that the G6-DNA can form dimers in aqueous solutions and G01-DNA can form additional G-quadruplex structures by binding to G6-DNA.However,G01-DNA has no effect on the structure of DNA with a sequence of d[AGGG(TTAGGG)3](G3-DNA).Our study provides new insights into the structure polymorphism of long human single-stranded telomeric DNA.
基金Project supported by the Ministry of Science and Technology of China (No. 2003CB415201)National Natural Science Foundation of China (No. 40671120)+1 种基金the International Atomic Energy Agency (Nos. 12322/RO and UK-12094)the Young Scientist Foundation of Sichuan Province (No.06ZQ026-030)
文摘Purple soils are widely distributed in the Sichuan Hilly Basin and are highly susceptible to erosion, especially on the cultivated slopes. Quantitative assessment of the erosion rates is, however, difficult due to small size of the plots of the manually-tilled land, the complex land use, and steep hillslopes. ^137Cs and ^210Pbex (excess ^210Pb) tracing techniques were used to investigate the spatial pattern of soil erosion rates associated with slope-land under hoe tillage in Neijiang of the Sichuan Hilly Basin. The ^137Cs and ^210Pbex inventories at the top of the cultivated slope were extremely low, and the highest inventories were found at the bottom of the cultivated slope. By combining the erosion rates estimates provided by both ^137Cs and 21~Pbex measurements, the weighted mean net soil loss from the study slope was estimated to be 3 100 t km^-2 year^-1, which was significantly less than 6 930 t km^-2 year^-1 reported for runoff plots on a 10° cultivated slope at the Suining Station of Soil Erosion. The spatial pattern of soil erosion rates on the steep agricultural land showed that hoe tillage played an important role in soil redistribution along the slope. Also, traditional farming practices had a significant role in reducing soil loss, leading to a lower net erosion rate for the field.
基金supported by the Open Research Fund of the State Key Laboratory of Complex Electromagnetic Environment Effects on Electronics and Information System(No.CEMEE2019Z0104B)。
文摘The problem of joint direction of arrival(DOA)and polarization estimation for polarization sensitive coprime planar arrays(PS-CPAs)is investigated,and a fast-convergence quadrilinear decomposition approach is proposed.Specifically,we first decompose the PS-CPA into two sparse polarization sensitive uniform planar subarrays and employ propagator method(PM)to construct the initial steering matrices separately.Then we arrange the received signals into two quadrilinear models so that the potential DOA and polarization estimates can be attained via quadrilinear alternating least square(QALS).Subsequently,we distinguish the true DOA estimates from the approximate intersecting estimations of the two subarrays in view of the coprime feature.Finally,the polarization estimates paired with DOA can be obtained.In contrast to the conventional QALS algorithm,the proposed approach can remarkably reduce the computational complexity without degrading the estimation performance.Simulations demonstrate the superiority of the proposed fast-convergence approach for PS-CPAs.
文摘The results from interpretation of the aerophotos and in-situ seismogeological researches show that there are some obvious late-Quaternary activities along the Moxi-Mianning segment of the Xianshuihe-Anninghe fault zone, with the characteristics of sinistral-slip movement accompanied by some significant vertical slip components. Since late-Quaternary, the average horizontal slip rate of the segment at the south of Moxi along the Xianshuihe fault is 6.0~9.9mm/a and 4.7~5.3mm/a along the segment at the north of Mianning of the Anninghe fault. The results from the investigation of coseismic dislocation and ground rupture show that the ground rupture caused by 1876 Kangding-Luding earthquake with M 7 3/ 4 can extend to the south of Tianwan. The segment at the north of Mianning of the Anninghe fault has a background for producing M7.5 earthquake and the geological record of the last strong earthquake must be the proofs of the 1327 earthquake with M>6.0 with poor historical records.
基金the National Natural Science Foundation of China (No. 29376233).
文摘Microparticle formation and crystallization rate of 1,3,5,7-tetranitro-l,3,5,7-tetraazacyclooctane (HMX) in acetone solution using supercritical carbon dioxide antisolvent (GAS) recrystallization were studied. Scanning electronic microscopy, X-ray diffraction and infrared radiation were used to examine particle size, crystallinity and chemical structure. The results show that B-HMX microparticle in different average size (2-9.5um) and with narrow size distribution were obtained by controlling the expansibility, expansion speed, initial concentration and temperature during recrystallization of HMX. The formation of nuclei may be a main cause of consumption of solute when the solution is expanded rapidly enough and the equilibrium concentration is lower, in which almost monodisperse microparticle can be obtained.
基金supported by the Special Fund for Agro-scientific Research in the Public Interest of China (No.201203013)the National Natural Science Foundation of China(Nos.40971176 and 40201027)the International Potash Institute(IPI) China Project
文摘Nonexchangeable K (NEK) is the major portion of the reserve of available K in soil and a primary factor in determining soil K fertility. The questions of how much NEK is in soils and how to quantify total NEK in soils are so far still unclear due to the complicated effects of various minerals on K fixation. In this study, the NEK in 9 soils was extracted with sodium tetraphenylboron (NaBPh4) for various time periods longer than 1 d. The results showed that the NEK extracted by NaBPh4 gradually increased with time, but showed no more increase after the duration of extraction exceeded 10 20 d. As the temperature increased from 25 to 45 ~C, the duration to obtain the maximum extraction of NEK was reduced from 20 to 10 d, and the maximum values of NEK released at both temperatures was almost the same for each soil. The maximum NEK (MNEK) of the 9 soils extracted by NaBPh4 varied from 3074 to 10081 mg kg-1, accounting for 21% 56% of the total soil K. There was no significant correlation between MNEK released by NaBPh4 and other forms of K, such as NH4OAc-extracted K, HNO3-extracted K and total K in soils, which indicates that NEK is a special form of K that has no inevitable relationship to the other forms of K in soils. The MNEK extraction by NaBPh4 in this study indicated that the total NEK in the soils could be differentiated from soil structural K and quantified with the modified NaBPh4 method. The high MNEK in soils made NEK much more important in the role of the plant-available K pool. How to fraetionate NEK into different fractions and establish the methods to quantify each NEK fraction according to their bioavailability is of great importance for future research.
基金supported by the National Natural Science Foundation of China (Grant Nos. 41472180 & 41172180)
文摘The Wenchuan earthquake occurred near the "triple junction" linking the Bayan Har block, the South China block, and the Sichuan-Yunnan rhombic block, and its influences on the surrounding blocks and the main fault zones in the Sichuan-Yunnan region, i.e., the block boundary zone, cannot be ignored. In this paper, changes of movement and stress of the fault zones before and after a strong earthquake were simulated based on the GPS repetition survey results recently obtained during 1999–2007, 2009–2011, and 2011–2013 with a two-dimensional finite-element contact model and the "block- loading" method. The results show that, before the Wenchuan earthquake, the movement of the Longmenshan fault zone was very slow and its compressive stress accumulated rapidly; after the Wenchuan earthquake, movements toward the E-SSE direction of the Bayan Har, southwestern Yunnan, and rhombic blocks were enhanced, and the dextral and horizontal compressive speeds and annual accumulative compressive stress of the Longmenshan fault zone increased markedly by factors of 4.5, 2.1, and 2.5, respectively. The southern Xianshuihe, Anninghe, Zemuhe, Daliangshan, and Lijiang-Xiaojinhe fault zones accumulated compressive stress rapidly, forming enhanced compressive stress zones along a NE strike crossing the central part of the Sichuan-Yunnan region. The tensional movement of the Xianshuihe fault zone was enhanced and the slip movement in the central part of the zone was reversed in a short time. The changes are tightly related to the medium-intensity earthquakes that occurred during the same period in this region, revealing that the spatial migration of seismic activity is related to changes of movement of the blocks.
基金supported by the National Natural Science Foundation of China(Grant No.41404048)the Seismological Research Project(Grant No.201108002)
文摘A crucial part of proposed earthquake early warning systems is a rapid estimate for earthquake magnitude.Most of these methods are focused on the first part of the P-wave train,the earlier and less destructive part of the ground motion that follows an earthquake.A method has been proposed by using the period of the P-wave to determine the magnitude of a large earthquake at local distance,and a specific relation for the Sichuan region was calibrated according to acceleration records of Wenchuan earthquake.The Mw 6.6 earthquake hit Lushan County,Sichuan,on April 20,2013 and the largest aftershocks provide a useful dataset to validate the proposed relation and discuss the risks connected to the extrapolation of magnitude relations with a poor dataset of large earthquake waveforms.A discrepancy between the local magnitude(ML)estimated by means ofτc evaluation and the standard ML(6.4 vs.7.0)suggests using caution when ML vs.τc calibrations do not include a relevant dataset of large earthquakes.Effects from large residuals could be mitigated or removed by introducing selection rules onτc function,by regionalizing the ML vs.τc function in the presence of significant tectonic or geological heterogeneity,and by using probabilistic and evolutionary methods.
基金supported by the National "Twelfth Five-Year" Plan for Science and Technology Support Program of China(No.2013BAD18B06)the Open Research Fund for the Key Laboratory of Dairy Science of Northeast Agricultural University(No.KLDS201201)the Innovative Research Team of Higher Education of Heilongjiang Province(No.2010td11),China
文摘Traditional methods for detecting lactoperoxidase (LP) are complex and time-consuming, so a test strip was made based on the enzymatic reaction principle to enable quick and convenient detection of LP in raw milk. In this study 0.1 mol/L citric acid (CA)/0.2 mol/L disodium hydrogen phosphate (NAP) buffer solution (pH 5.0), 22 mmol/L 3,3',5,5'-tetramethylbenzidine (TMB), 0.6 mmol/L hydrogen peroxide (H202), and 0.5% Tween-20 or 0.3% cetyltri- methyl ammonium bromide (CTAB) were optimal for preparing a quick, sensitive, and accurate LP test strip. The coefficient of variation (CV) of the estimated LP concentrations ranged from 2.47% to 6.72% and the minimum LP concentration detected by the test strip was 1-2 mg/L. Estimates of active LP in sixteen raw milk samples obtained using the test strip or the TMB method showed a good correlation (c=0.9776). So the test strip provides a quick, convenient, and accurate method for detecting the LP concentration of raw milk.
基金supported by the National Basic Research Program of China(Grant No.2012CB720101)the National Natural Science Foundation of China(Grant No.10832001)the Opening Subject of State Key Laboratory of Nonlinear Mechanics,Institute of Mechanics,Chinese Academy of Sciences
文摘In this paper,we calculated the spatial local-averaged velocity strains along the streamwise direction at four spatial scales according to the concept of spatial local-averaged velocity structure function by using the three-dimensional three-component database of time series of velocity vector field in the turbulent boundary layer measured by tomographic time-resolved particle image velocimetry.An improved quadrant splitting method,based on the spatial local-averaged velocity strains together with a new conditional sampling phase average technique,was introduced as a criterion to detect the coherent structure topology.Furthermore,we used them to detect and extract the spatial topologies of fluctuating velocity and fluctuating vorticity whose center is a strong second-quadrant event(Q2) or a fourth-quadrant event(Q4).Results illustrate that a closer similarity of the multi-scale coherent structures is present in the wall-normal direction,compared to the one in the other two directions.The relationship among such topological coherent structures and Reynolds stress bursting events,as well as the fluctuating vorticity was discussed.When other burst events are surveyed(the first-quadrant event Q1 and the third-quadrant event Q3),a fascinating bursting period circularly occurs:Q4-S-Q2-Q3-Q2-Q1-Q4-S-Q2-Q3-Q2-Q1 in the center of such topological structures along the streamwise direction.In addition,the probability of the Q2 bursting event occurrence is slightly higher than that of the Q4 event occurrence.The spatial instable singularity that almost simultaneously appears together with typical Q2 or Q4 events has been observed,which is the main character of the mutual induction mechanism and vortex auto-generation mechanism explaining how the turbulence is produced and maintained.