Xishuangbanna is a multinational region and different ethnic groups have self-owned traditional ways of protecting biodiversity, represented by Dragon Moun-tain and Hil Cemetery, and Temple Forest. As population incre...Xishuangbanna is a multinational region and different ethnic groups have self-owned traditional ways of protecting biodiversity, represented by Dragon Moun-tain and Hil Cemetery, and Temple Forest. As population increases and economy develops, people’s consciousness of traditional culture is fading and both of the number and area of sacred natural sites underwent changes, undermining the role of sacred natural sites playing in biodiversity protection, especial y for species pro-tection and gene exchange.展开更多
In the framework of the Santiago of Compostela Cathedral program, a multidisciplinary investigation of the porch of the glory was carried out between 2009 and 2011 to identify the main environmental risks and to devel...In the framework of the Santiago of Compostela Cathedral program, a multidisciplinary investigation of the porch of the glory was carried out between 2009 and 2011 to identify the main environmental risks and to develop a preventive conservation planto be integrated in the general management strategy of the Cathedral. The study included historic and archivist research, structural studies, mineralogical analyses, biological sampling, cleaning tests and microclimatic monitoring. The main weathering factors and the related damage processes were identified. Results have shown that the main responsible for the observed damage was the infiltration of rainwater through the roof, due to cracks in the structure of the Cathedral. Other environmental factors having a remarkable impact on the state of conservation of the polychrome and its substrate were the solar radiation, the thermo-hygrometric cycles, the particle deposition and the biological growth. Solutions were suggested to improve the environmental conditions, thus reducing further damage.展开更多
In our previous study, we have elucidated the chemical profile ofYGS40, a fraction of Yi-Gan San （YGS）, used for the treatment of Alzheimer＇s disease （AD）. Oxidative stress-induced apoptosis is implicated in neur...In our previous study, we have elucidated the chemical profile ofYGS40, a fraction of Yi-Gan San （YGS）, used for the treatment of Alzheimer＇s disease （AD）. Oxidative stress-induced apoptosis is implicated in neurodegenerative disorders such as AD. The aim of the present study was to explore the protective effects of YGS40 against hydrogen peroxide （H202）-induced apoptosis in PC12 cells and the underlying mechanisms. PC12 cells were exposed to 100 μmol·L 1 of H202 for 12 h with or without YGS40 pretreatment. Cytotoxicity was determined by MTT （3, （4, 5-dimethylthiazole-2-yl） 2, 5-diphenyl-tetrazolium bromide） and lactate dehydrogenase （LDH） release assays; apoptosis was detected by Annexin V/propidium iodide coupled staining and by determining caspase-3 activity and Bax and Bcl-2 protein levels. Mitochondrial membrane potential （MMP） was assessed by the retention of rhodamine123; and the activities of superoxide dismutase （SOD） and malondialdehyde （MDA） were measured using commercially available enzymatic kits. Pretreatment with YGS40 significantly prevented H2O2-induced cytotoxicity and protected the cells against H2O2-triggered apoptosis characterized by extemalization of membrane phosphatidylserine and caspase-3 activation and the increased ratio of Bax/Bcl-2 in PC12 cells. Further studies showed that YGS40 suppressed H2O2-induced MMP loss, increased SOD activity, and decreased MDA level. These findings suggest that YGS40 may be beneficial for the prevention and treatment of oxidative stress-mediated disorders.展开更多
基金Supported by Xishuangbanna Tropic Rain Forest Conservation Foundation~~
文摘Xishuangbanna is a multinational region and different ethnic groups have self-owned traditional ways of protecting biodiversity, represented by Dragon Moun-tain and Hil Cemetery, and Temple Forest. As population increases and economy develops, people’s consciousness of traditional culture is fading and both of the number and area of sacred natural sites underwent changes, undermining the role of sacred natural sites playing in biodiversity protection, especial y for species pro-tection and gene exchange.
文摘In the framework of the Santiago of Compostela Cathedral program, a multidisciplinary investigation of the porch of the glory was carried out between 2009 and 2011 to identify the main environmental risks and to develop a preventive conservation planto be integrated in the general management strategy of the Cathedral. The study included historic and archivist research, structural studies, mineralogical analyses, biological sampling, cleaning tests and microclimatic monitoring. The main weathering factors and the related damage processes were identified. Results have shown that the main responsible for the observed damage was the infiltration of rainwater through the roof, due to cracks in the structure of the Cathedral. Other environmental factors having a remarkable impact on the state of conservation of the polychrome and its substrate were the solar radiation, the thermo-hygrometric cycles, the particle deposition and the biological growth. Solutions were suggested to improve the environmental conditions, thus reducing further damage.
基金supported by the National Natural Science Foundation of China(Nos.81274046 and 81373956)
文摘In our previous study, we have elucidated the chemical profile ofYGS40, a fraction of Yi-Gan San （YGS）, used for the treatment of Alzheimer＇s disease （AD）. Oxidative stress-induced apoptosis is implicated in neurodegenerative disorders such as AD. The aim of the present study was to explore the protective effects of YGS40 against hydrogen peroxide （H202）-induced apoptosis in PC12 cells and the underlying mechanisms. PC12 cells were exposed to 100 μmol·L 1 of H202 for 12 h with or without YGS40 pretreatment. Cytotoxicity was determined by MTT （3, （4, 5-dimethylthiazole-2-yl） 2, 5-diphenyl-tetrazolium bromide） and lactate dehydrogenase （LDH） release assays; apoptosis was detected by Annexin V/propidium iodide coupled staining and by determining caspase-3 activity and Bax and Bcl-2 protein levels. Mitochondrial membrane potential （MMP） was assessed by the retention of rhodamine123; and the activities of superoxide dismutase （SOD） and malondialdehyde （MDA） were measured using commercially available enzymatic kits. Pretreatment with YGS40 significantly prevented H2O2-induced cytotoxicity and protected the cells against H2O2-triggered apoptosis characterized by extemalization of membrane phosphatidylserine and caspase-3 activation and the increased ratio of Bax/Bcl-2 in PC12 cells. Further studies showed that YGS40 suppressed H2O2-induced MMP loss, increased SOD activity, and decreased MDA level. These findings suggest that YGS40 may be beneficial for the prevention and treatment of oxidative stress-mediated disorders.
