[Objective] The study aimed to provide theoretical basis for development and application of molecular marker breeding technique to obtain Bombyx mori near-isogenic lines （NILs）. [Method] Thermotolerance gene was int...[Objective] The study aimed to provide theoretical basis for development and application of molecular marker breeding technique to obtain Bombyx mori near-isogenic lines （NILs）. [Method] Thermotolerance gene was introduced into sensitive variety Ou17 by developing NILs and recurrent backcross,then through six generations of backcross,thermotolerance-assisted selection,and two generations of self-cross. [Result] Bombyx mori NILs carrying thermotolerance gene （new germplasm） were produced. Meanwhile,thermotolerance level of progenies of each backcross and molecular markers of NILs were determined,and then attempts were made to produce practical thermotolerance hybrids by using thermotolerance varieties whose thermotolerance gene is linked to SSR markers. [Conclusion] The study successfully construct thermotolerance NILs,monitor thermotolerance level and breeding results of progenies of each backcross,and determine molecular marker of NILs.展开更多
Tomato seedlings were grown in substrate culture with pots. The formulation of Holland Greenhouse Horticulture Research Institute was used(as CK) and the effects of different Ca concentrations(LCa, CK and HCa)on growt...Tomato seedlings were grown in substrate culture with pots. The formulation of Holland Greenhouse Horticulture Research Institute was used(as CK) and the effects of different Ca concentrations(LCa, CK and HCa)on growth, yield, and fruit quality(protein, Vitamin C, nitrates, organic acid and soluble sugar) of tomato were studied keeping concentrations of other nutrients unchanged in the nutrient solution. The results showed that parameters related to the growth of tomato(plant height and Stem diameter), changes of tomato yield per plant and quality of tomato fruits were the highest when the plants were grown at 20% Ca treatments. In the second study, increased EC concentrations of nutrient solution resulted in stronger plants with improved yields and quality. Four different concentration gradients of nutrient solution treatment were designed based on the results of the first research stage(EC=1.5, 2.5, 3.5, 4.5 m S/cm, respectively). The single tomato plant had the highest production which is 2 268.994 g/plant, when the nutrient solution strength was at EC=1.5 m S cm, whereas they have the best fruit quality when the solution strength at EC 4.5 m S/cm. This suggests the need for wide popularization of the nutrient solution formula in large areas to improve the tomato production.展开更多
P SAG12 _ IPT gene was introduced into an elite rice (Oryza sativa L. ssp. indica ) restorer line Minghui 63 through Agrobacterium _mediated transformation method. Out of 61 independent transgenic plants ...P SAG12 _ IPT gene was introduced into an elite rice (Oryza sativa L. ssp. indica ) restorer line Minghui 63 through Agrobacterium _mediated transformation method. Out of 61 independent transgenic plants obtained, a few acquired a recognizable phenotype in which leave senescence was delayed to a great degree. The results of field plot test on two homozygous transgenic lines indicated: (1) the stay_green ability of transgenic plants was significantly improved; (2) both the seed_setting rate and the number of panicles per plant of transgenic plants were significantly increased compared with that of the non_transgenic plants of Minghui 63; and (3) the plant height of transgenic plants was significantly reduced.展开更多
Cultured Apostichopus japonicus in China suffers from a kind of skin ulceration disease that has caused severe economic loss in recent years. The disease,pathogens of which are supposed to be bacteria by most research...Cultured Apostichopus japonicus in China suffers from a kind of skin ulceration disease that has caused severe economic loss in recent years. The disease,pathogens of which are supposed to be bacteria by most researchers,is highly infectious and can often cause all individuals in the same culture pool to die in a very short time. The 16S rRNA gene phylogenesis of the culturable bacteria from the lesions of diseased individuals was conducted to study the biodiversity of the bacterial communities in the lesions and to identify probable pathogen(s) associated with this kind of disease. S. japonica samples were selected from a hatchery located in the eastern part of Qingdao,China. Bacterial universal primers GM5F and DS907R were used to amplify the 16S rRNA gene of bacteria colonies,and touchdown PCR was performed to amplify the target sequences. The results suggest that γ-proteobacteria(Alteromonadales and Vibrionales) of CFB group,many strains of which have been also determined as pathogens in other marine species,are the predominant bacterial genera of the diseased Apostichopus japonicus individuals.展开更多
This paper conducts the probe into the methods of implementing the general education in application-oriented civilian-run colleges and universities. As the basic education for the construction of high-quality teachers...This paper conducts the probe into the methods of implementing the general education in application-oriented civilian-run colleges and universities. As the basic education for the construction of high-quality teachers more and more urgent, how to make application-oriented colleges and universities teacher education in the personnel training level and training mode to meet the basic education development needs. Therefore, accomplishes the counted by the 100 million high quality worker diligently, ten million counts the professional personnel and a large quantities of best pick of the crop innovation talented person, specially raises the considerable amount to stress in the practical work department on the solution actual problem high level. Compared with the state-of-the-art core applications, this paper’s idea is novel and effective.展开更多
Hypersaline environments are colonized by communities of microorganisms, which developed adaptations from halotolerant to extreme halophilic lifestyles. Twenty-four halophilic archaeal strains were isolated from three...Hypersaline environments are colonized by communities of microorganisms, which developed adaptations from halotolerant to extreme halophilic lifestyles. Twenty-four halophilic archaeal strains were isolated from three Algerian hypersaline sites, including the solar salterns in Ichekaben and two natural salt lakes at Ouargla and Oran. Almost full-length 16S rRNA genes were amplified using archaeal-specific primers, sequenced and phylogenetically analyzed. Three isolates from the salt lake of Ouargla were affiliated to Halorubrum sp., while six isolates from Ichekaben were Natrinema sp.. The remaining strains belonged to the genus Haloarcula, which was identified in the three Algerian sites. A phylogenetic tree of Haloarcula sp. including the two copies of the 16S rRNA gene, showed that two strains group with Haloarcula hispanica, while the other 13 cluster with OHF-1/OHF-2 isolated from French commercial salt. Both Natrinema sp. and Haloarcula sp. were persistent at Ichekaben. For the six Natrinema sp. isolates, this observation correlates with their capacity to produce antimicrobial compounds, and a DNA fragment closely related to the halocin C8 gene was amplified for five strains.展开更多
Chinese Contemporary Literature as related professional foundation courses, with the development of the country＇ s higher education reform, its status and role are also changing and Under the current talents to profe...Chinese Contemporary Literature as related professional foundation courses, with the development of the country＇ s higher education reform, its status and role are also changing and Under the current talents to professional competence based values, many students of Chinese Modem Literature awareness and interest gradually weakened, due to reduced hours, the old teaching model and the impact on consumer attitudes about fast food culture, college students thinking of contemporary Chinese literature, values, and aesthetic taste, are a significant deviation and dislocation. To this end, we should strengthen the status of contemporary Chinese literature teaching, and teaching literature from many problems currently faced by the contemporary university, and re-examine their academic, cultural, informative features, innovative teaching model to promote the current inherit Chinese Contemporary Literature Teaching and development.展开更多
基金Supported by Jiangsu High-tech projects(BG2007322) "Good feature of new varieties of Silkworm BreedingPh.D., University of Jiangsu Science and Technology Fund~~
文摘[Objective] The study aimed to provide theoretical basis for development and application of molecular marker breeding technique to obtain Bombyx mori near-isogenic lines （NILs）. [Method] Thermotolerance gene was introduced into sensitive variety Ou17 by developing NILs and recurrent backcross,then through six generations of backcross,thermotolerance-assisted selection,and two generations of self-cross. [Result] Bombyx mori NILs carrying thermotolerance gene （new germplasm） were produced. Meanwhile,thermotolerance level of progenies of each backcross and molecular markers of NILs were determined,and then attempts were made to produce practical thermotolerance hybrids by using thermotolerance varieties whose thermotolerance gene is linked to SSR markers. [Conclusion] The study successfully construct thermotolerance NILs,monitor thermotolerance level and breeding results of progenies of each backcross,and determine molecular marker of NILs.
文摘Tomato seedlings were grown in substrate culture with pots. The formulation of Holland Greenhouse Horticulture Research Institute was used(as CK) and the effects of different Ca concentrations(LCa, CK and HCa)on growth, yield, and fruit quality(protein, Vitamin C, nitrates, organic acid and soluble sugar) of tomato were studied keeping concentrations of other nutrients unchanged in the nutrient solution. The results showed that parameters related to the growth of tomato(plant height and Stem diameter), changes of tomato yield per plant and quality of tomato fruits were the highest when the plants were grown at 20% Ca treatments. In the second study, increased EC concentrations of nutrient solution resulted in stronger plants with improved yields and quality. Four different concentration gradients of nutrient solution treatment were designed based on the results of the first research stage(EC=1.5, 2.5, 3.5, 4.5 m S/cm, respectively). The single tomato plant had the highest production which is 2 268.994 g/plant, when the nutrient solution strength was at EC=1.5 m S cm, whereas they have the best fruit quality when the solution strength at EC 4.5 m S/cm. This suggests the need for wide popularization of the nutrient solution formula in large areas to improve the tomato production.
文摘P SAG12 _ IPT gene was introduced into an elite rice (Oryza sativa L. ssp. indica ) restorer line Minghui 63 through Agrobacterium _mediated transformation method. Out of 61 independent transgenic plants obtained, a few acquired a recognizable phenotype in which leave senescence was delayed to a great degree. The results of field plot test on two homozygous transgenic lines indicated: (1) the stay_green ability of transgenic plants was significantly improved; (2) both the seed_setting rate and the number of panicles per plant of transgenic plants were significantly increased compared with that of the non_transgenic plants of Minghui 63; and (3) the plant height of transgenic plants was significantly reduced.
文摘Cultured Apostichopus japonicus in China suffers from a kind of skin ulceration disease that has caused severe economic loss in recent years. The disease,pathogens of which are supposed to be bacteria by most researchers,is highly infectious and can often cause all individuals in the same culture pool to die in a very short time. The 16S rRNA gene phylogenesis of the culturable bacteria from the lesions of diseased individuals was conducted to study the biodiversity of the bacterial communities in the lesions and to identify probable pathogen(s) associated with this kind of disease. S. japonica samples were selected from a hatchery located in the eastern part of Qingdao,China. Bacterial universal primers GM5F and DS907R were used to amplify the 16S rRNA gene of bacteria colonies,and touchdown PCR was performed to amplify the target sequences. The results suggest that γ-proteobacteria(Alteromonadales and Vibrionales) of CFB group,many strains of which have been also determined as pathogens in other marine species,are the predominant bacterial genera of the diseased Apostichopus japonicus individuals.
文摘This paper conducts the probe into the methods of implementing the general education in application-oriented civilian-run colleges and universities. As the basic education for the construction of high-quality teachers more and more urgent, how to make application-oriented colleges and universities teacher education in the personnel training level and training mode to meet the basic education development needs. Therefore, accomplishes the counted by the 100 million high quality worker diligently, ten million counts the professional personnel and a large quantities of best pick of the crop innovation talented person, specially raises the considerable amount to stress in the practical work department on the solution actual problem high level. Compared with the state-of-the-art core applications, this paper’s idea is novel and effective.
文摘Hypersaline environments are colonized by communities of microorganisms, which developed adaptations from halotolerant to extreme halophilic lifestyles. Twenty-four halophilic archaeal strains were isolated from three Algerian hypersaline sites, including the solar salterns in Ichekaben and two natural salt lakes at Ouargla and Oran. Almost full-length 16S rRNA genes were amplified using archaeal-specific primers, sequenced and phylogenetically analyzed. Three isolates from the salt lake of Ouargla were affiliated to Halorubrum sp., while six isolates from Ichekaben were Natrinema sp.. The remaining strains belonged to the genus Haloarcula, which was identified in the three Algerian sites. A phylogenetic tree of Haloarcula sp. including the two copies of the 16S rRNA gene, showed that two strains group with Haloarcula hispanica, while the other 13 cluster with OHF-1/OHF-2 isolated from French commercial salt. Both Natrinema sp. and Haloarcula sp. were persistent at Ichekaben. For the six Natrinema sp. isolates, this observation correlates with their capacity to produce antimicrobial compounds, and a DNA fragment closely related to the halocin C8 gene was amplified for five strains.
文摘Chinese Contemporary Literature as related professional foundation courses, with the development of the country＇ s higher education reform, its status and role are also changing and Under the current talents to professional competence based values, many students of Chinese Modem Literature awareness and interest gradually weakened, due to reduced hours, the old teaching model and the impact on consumer attitudes about fast food culture, college students thinking of contemporary Chinese literature, values, and aesthetic taste, are a significant deviation and dislocation. To this end, we should strengthen the status of contemporary Chinese literature teaching, and teaching literature from many problems currently faced by the contemporary university, and re-examine their academic, cultural, informative features, innovative teaching model to promote the current inherit Chinese Contemporary Literature Teaching and development.
