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“小词”考述 被引量:1
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作者 许兴宝 《新疆大学学报(社会科学版)》 CSSCI 北大核心 2003年第4期96-101,共6页
“小词”在宋人词话中出现频率颇高,其内涵是指词体篇幅短小者,并非宋人给词附加的蔑称。宋人以小为美,将绝句也称作小诗,还有更多以“小”称物的语言现象,因此,小词与小诗表达了宋人同样的文化心态。
关键词 宋朝 “小词” “小曲” “小品”
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Reference information time delay estimation and compensation in transfer alignment 被引量:2
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作者 陆源 程向红 《Journal of Southeast University(English Edition)》 EI CAS 2011年第1期52-55,共4页
To reduce the error in transfer alignment caused by reference information delay,a time delay estimation method is developed based on least-squares curve fitting of the angular rate integration.First,the gyro sensor me... To reduce the error in transfer alignment caused by reference information delay,a time delay estimation method is developed based on least-squares curve fitting of the angular rate integration.First,the gyro sensor measurements of the main strapdown inertial navigation system(M-SINS) and the slave strapdown inertial navigation system(S-SINS) are recorded for a few seconds and the integration of the data is calculated.Then,the possible maximum range of the delay value is defined and the points of the curve at different intervals are moved.The square of the differences between the corresponding points are calculated.Finally,the delay estimation can be acquired by the least-squares curve fitting of the M-SINS and the S-SINS.A delay compensation method by local data shifting is also presented.The simulation results demonstrate the effectiveness of delay estimation and indicate that the estimation accuracy is independent of the delay value.And the local data shifting compensation method can effectively reduce the errors of the transfer alignment caused by the reference information delay. 展开更多
关键词 transfer alignment DELAY ESTIMATION COMPENSATION SYNCHRONIZATION least squares curve fitting
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Relationship between Bending Property and Density of Wheat Stem 被引量:3
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作者 袁志华 李英俊 《Agricultural Science & Technology》 CAS 2009年第5期100-101,共2页
[ Objective] The aim is to research the relationship between bending property and density of wheat stem. [ Method ] The bending properties such as elastic modulus, bending strength, flexural rigidity, moment of inerti... [ Objective] The aim is to research the relationship between bending property and density of wheat stem. [ Method ] The bending properties such as elastic modulus, bending strength, flexural rigidity, moment of inertia, density and water content of the second base internodes of Zhengmai 9023 and Yumai 25 were determined. [ Result] The results show that during filling stage, there are significant differences in the elastic modulus, moment of inertia, flexural rigidity and density among wheat varieties, while there are no significant differences in the bending strength and water content among wheat varieties. The moment of inertia, flexural strength and flexural rigidity have positive relationship to density but negative relationship to water content. [ Conclusion] The study results provide some references for the research on high yield cultivation and lodging resistance of wheat. 展开更多
关键词 Wheat stem Bending property DENSITY Water content
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A study of wavelet transforms applied for fracture identification and fracture density evaluation 被引量:3
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作者 张晓峰 潘保芝 +1 位作者 王飞 韩雪 《Applied Geophysics》 SCIE CSCD 2011年第2期164-169,178,179,共8页
Combining wavelet transforms with conventional log differential curves is used to identify fractured sections is a new idea.In this paper,we first compute the mother wavelet transform of conventional logs and the wave... Combining wavelet transforms with conventional log differential curves is used to identify fractured sections is a new idea.In this paper,we first compute the mother wavelet transform of conventional logs and the wavelet decomposed signals are compared with fractures identified from image logs to determine the fracture-matched mother wavelet.Then the mother wavelet-based decomposed signal combined with the differential curves of conventional well logs create a fracture indicator curve,identifying the fractured zone.Finally the fracture density can be precisely evaluated by the linear relationship of the indicator curve and image log fracture density.This method has been successfully used to evaluate igneous reservoir fractures in the southern Songnan basin and the calculated density from the indicator curve and density from image logs are both basically consistent. 展开更多
关键词 Wavelet transform fracture identification differential curves fracture density
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WEIGHTED LEAST SQUARE METHOD FOR S-N CURVE FITTING 被引量:7
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作者 吉凤贤 姚卫星 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2004年第1期53-57,共5页
An S-N curve fitting approach is proposed based on the weighted least square method, and the weights are inversely proportional to the length of mean confidence intervals of experimental data sets. The assumption coin... An S-N curve fitting approach is proposed based on the weighted least square method, and the weights are inversely proportional to the length of mean confidence intervals of experimental data sets. The assumption coincides with the physical characteristics of the fatigue life scatter. Two examples demonstrate the method. It is shown that the method has better accuracy and reasonableness compared with the usual least square method. 展开更多
关键词 fatigue test S-N curve weighted least square method confidence interval
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Control mesh representation of a class of minimal surfaces 被引量:4
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作者 XU Gang WANG Guo-zhao 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2006年第9期1544-1549,共6页
Minimal surface is extensively employed in many areas. In this paper, we propose a control mesh representation of a class of minimal surfaces, called generalized helicoid minimal surfaces, which contain the right heli... Minimal surface is extensively employed in many areas. In this paper, we propose a control mesh representation of a class of minimal surfaces, called generalized helicoid minimal surfaces, which contain the right helicoid and catenoid as special examples. We firstly construct the Bézier-like basis called AHT Bézier basis in the space spanned by {1, t, sint, cost, sinht, cosht}, t∈[0,α], α∈[0,5π/2]. Then we propose the control mesh representation of the generalized helicoid using the AHT Bézier basis. This kind of representation enables generating the minimal surfaces using the de Casteljau-like algorithm in CAD/CAGD mod- elling systems. 展开更多
关键词 Minimal surface Helicoid surface Catenoid Control mesh
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Significance-based optimization of processing parameters for thin-walled aluminum alloy tube NC bending with small bending radius 被引量:13
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作者 XU Jie YANG He +1 位作者 LI Heng ZHAN Mei 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2012年第1期147-156,共10页
Thin-walled aluminum alloy tube numerical control (NC) bending with small bending radius is a complex process with multi-factor coupling effects and multi-die constraints. A significance-based optimization method of... Thin-walled aluminum alloy tube numerical control (NC) bending with small bending radius is a complex process with multi-factor coupling effects and multi-die constraints. A significance-based optimization method of the parameters was proposed based on the finite element (FE) simulation, and the significance analysis of the processing parameters on the forming quality in terms of the maximum wall thinning ratio and the maximum cross section distortion degree was implemented using the fractional factorial design. The optimum value of the significant parameter, the clearance between the tube and the wiper die, was obtained, and the values of the other parameters, including the friction coefficients and the clearances between the tube and the dies, the mandrel extension length and the boost velocity were estimated. The results are applied to aluminum alloy tube NC bending d50 mm×1 mm×75 mm and d70 mm×1.5 mm×105 mm (initial tube outside diameter D0 × initial tube wall thickness t0 × bending radius R), and qualified tubes are produced. 展开更多
关键词 thin-walled aluminum alloy tube OPTIMIZATION finite element (FE) numerical control bending processing parameters significance analysis small bending radius
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Post-Buckling Analysis for Elastic Thin Plate Under Unilateral Frictionless Restraint 被引量:1
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作者 张贇 程兆雄 《Journal of Beijing Institute of Technology》 EI CAS 2001年第1期34-38,共5页
To investigate the buckling and post buckling behaviors of elastic thin plate under frictionless unilateral restraint, enduring the coupling action of lognitudinal and transverse loads, the principle of minimum poten... To investigate the buckling and post buckling behaviors of elastic thin plate under frictionless unilateral restraint, enduring the coupling action of lognitudinal and transverse loads, the principle of minimum potential energy and variational method are used and series functions with unknown coefficients are taken as trial functions of functional to solve the large deflection and non linear bending problem of a thin plate and find relation curves between deflection of plate and loads. The proposed method can capture the buckling and post buckling behaviors of a thin plate in different geometrical and load boundary conditions. The analysis confirms that there occur snap and bifurcation behaviors in the post buckling stage of the plate. And these results show the validity of the variational method for solving buckling problems of thin plate. 展开更多
关键词 thin plate post buckling variational method the principle of minimum potential energy
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Long-term results of small-diameter proximal splenorenal venous shunt:A retrospective study 被引量:4
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作者 Hao Chen Wei-Ping Yang Ji-Qi Yan Qin-Yu Li Di Ma Hong-Wei Li 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第29期3453-3458,共6页
AIM:To investigate recurrent variceal hemorrhage and long-term survival rates of patients treated with partial proximal splenorenal venous shunt.METHODS:Patients with variceal hemorrhage who were treated with small-di... AIM:To investigate recurrent variceal hemorrhage and long-term survival rates of patients treated with partial proximal splenorenal venous shunt.METHODS:Patients with variceal hemorrhage who were treated with small-diameter proximal splenorenal venous shunt in Ruijin Hospital between 1996 and 2009 were included in this study.Shunt diameter was determined before operation using Duplex Doppler ultrasonography.Peri-operative and long-term results in term of rehemorrhage,encephalopathy and mortality were followed up.RESULTS:Ninety-eight patients with Child A and B variceal hemorrhage received small-diameter proximal splenorenal venous shunt with a diameter of 7-10 mm.After operation,the patients’mean free portal pressure (P<0.01)and the flow rate of main portal vein(P< 0.01)decreased significantly compared with that before operation.The rates of rebleeding and mortality were 6.12%(6 cases)and 2.04%(2 cases),respectively.Ninety-one patients were followed up for 7 mo-14 years (median,48.57 mo).Long-term rates of rehemorrhage and encephalopathy were 4.40%(4 cases)and 3.30% (3 cases),respectively.Thirteen patients(14.29%)died mainly due to progressive hepatic dysfunction.Fiveand ten-year survival rates were 82.12%and 71.24%,respectively.CONCLUSION:Small-diameter proximal splenorenal venous shunt affords protection against variceal rehemorrhage with a low occurrence of encephalopathy in patients with normal liver function. 展开更多
关键词 Partial portacaval shunt HEMORRHAGE Esophageal varices Shunt diameter ENCEPHALOPATHY
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Lactobacillus crispatus M206119 exacerbates murine DSS-colitis by interfering with inflammatory responses 被引量:3
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作者 Fu-Xi Zhou Lu Chen +5 位作者 Xiao-Wei Liu Chun-Hui Ouyang Xiao-Ping Wu Xue-Hong Wang Chun-Lian Wang Fang-Gen Lu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第19期2344-2356,共13页
AIM: TO investigate the role of Lactobacillus crispatus (L. crispatus) strain China Center for Type Culture Col- lection (CCTCC) M206119 in intestinal inflammation.METHODS: Forty 8-wk-old Balb/c mice (20± ... AIM: TO investigate the role of Lactobacillus crispatus (L. crispatus) strain China Center for Type Culture Col- lection (CCTCC) M206119 in intestinal inflammation.METHODS: Forty 8-wk-old Balb/c mice (20± 2 g) were divided into four groups of 10 mice each. Three groups that had received dextran sulfate sodium (DSS) were administered normal saline, sulfasalazine or CCTCC M206119 strain, and the fourth group received none of these. We assessed the severity of colitis using a disease activity index, measured the colon length and weight, collected stools and mesenteric lymph nodes for bacterial microflora analysis. One centimeter of the proximal colon, middle colon and distal colon were collected and fixed in 10% buffered formalin, dehydrated in ethanol, and embedded in paraffin. Interleukin (IL)- 1β, IL-6 and tumor necrosis factor (TNF)-α expression was detected using reverse transcription polymerase chain reaction. Protective factors zonula occludens (ZO)-1 and β-defensin 2 were detected by immunoblot-ting. The features of CCTCC M206119 strain were identified based on morphology, biochemical profile, and 16S RNA sequencing.RESULTS: DSS-colitis animals treated with CCTCC M206119 had markedly more severe disease, with greater weight loss, diarrhea, fecal bleeding, and shortened colon length. In addition, the CCTCC-M206119- treated group had comparatively higher histologi- cal scores and more neutrophil infiltration than the controls. Expression of protective factors ZO-1 and β-defensin 2 was downregulated due to destruction of the mucosal barrier after CCTCC M206119 strain treatment. An in vitro assay demonstrated that CCTCC M206119 strain increased the nuclear translocation of nuclear factor-κB in epithelial cells. Intestinal proinflam- matory or anti-inflammatory cytokine responses were evaluated. Proinflammatory colonic cytokine (IL-Iβ, IL-6 and TNF-α) levels were clearly increased in CCTCC- M206119-treated animals, whereas anti-inflammatory colonic cytokine (IL-10) level was lowered compared with saline or 5-aminosalicylic-acid-treated DSS-colitis mice. Next, CCTCC M206119 strain was characterized as 1. crispatus by microscopic morphology, biochemical tests and 16S rRNA gene level.CONCLUSION: Not all lactobacilli are beneficial for in- testinal inflammation, and L. crispatus CCTCC M206119 strain is involved in exacerbation of intestinal inflamma- tion in DSS-colitis mice. 展开更多
关键词 COLITIS Lactobacillus crispatus INTESTINE Dextra sodium sulfate MICE
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Statistical evaluation of five failure criteria for intact salt rock 被引量:2
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作者 马林建 刘新宇 +1 位作者 方秦 马淑娜 《Journal of Central South University》 SCIE EI CAS 2011年第3期925-931,共7页
Five multiparameter empirical criteria were exclusively evaluated by comparing them with the strength data covering various stress conditions to find out which failure criterion best fits the test data and describes t... Five multiparameter empirical criteria were exclusively evaluated by comparing them with the strength data covering various stress conditions to find out which failure criterion best fits the test data and describes the mechanical behavior of the salt rock sequence (halite,bedded composite specimens and anhydrite interlayers).Full-scale comparison of all criteria for the three rock types was conducted based on five standard statistics calculated from least squares curve-fitting,which measures both the goodness of fitting and the quality of future prediction.The results indicate that all five nonlinear criteria with a basic power form are efficient in predicting the strength trend in the low tension area as well as in the high compression area of the soft rocks.The parameters obtained for the bedded rock salt are somewhat in the ones for the "pure" rocks and are even closer to those obtained for the halite.The generalized Hoek-Brown criterion is proven to perform best to two rock strength data followed by one for the Bieniawski empirical criterion,thus is the best candidate for the analysis of the salt rock.The Sheorey empirical criterion consistently achieves an intermediate performance for all the three rocks.It seems that the superiority of the poly-axial criteria (the Mogi 1967 criterion and the N-type criterion) over the former three triaxial criteria no longer exists when applied to the conventional triaxial strength data.Besides,the method of tension cut-off was proposed to solve the ambiguity problem of the two poly-axial criteria in the tension field in the plane of the major (σ1) andminor principal stress (σ3). 展开更多
关键词 salt rock sequence bedded rock salt failure criterion curve fitting
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Calculation of stratum surface principal curvature based on a moving least square method 被引量:2
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作者 LI Guo-qing MENG Zhao-ping +4 位作者 MA Feng-shan ZHAO Hai-jun DING De-min LIU Qin WANG Cheng 《Journal of China University of Mining and Technology》 EI 2008年第1期59-63,共5页
With the east section of the Changji sag Zhunger Basin as a case study, both a principal curvature method and a moving least square method are elaborated. The moving least square method is introduced, for the first ti... With the east section of the Changji sag Zhunger Basin as a case study, both a principal curvature method and a moving least square method are elaborated. The moving least square method is introduced, for the first time, to fit a stratum surface. The results show that, using the same-degree base function, compared with a traditional least square method, the moving least square method can produce lower fitting errors, the fitting surface can describe the morphological characteristics of stratum surfaces more accurately and the principal curvature values vary within a wide range and may be more suitable for the prediction of the distribution of structural fractures. The moving least square method could be useful in curved surface fitting and stratum curvature analysis. 展开更多
关键词 principal curvatures moving least square method surface fitting structural fractures
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Use of Horton Infiltration Model in Estimating Infiltration Characteristics of an Alfisol in the Northern Guinea Savanna of Nigeria 被引量:2
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作者 A. Abdulkadir M. N. Wuddivira +1 位作者 N. Abdu O. J. Mudiare 《Journal of Agricultural Science and Technology(A)》 2011年第6期925-931,共7页
Field infiltration measurement is often a tedious task thus can be easily estimated from proposed infiltration models. The Horton equation is one of the popular models used in the characterization of field infiltratio... Field infiltration measurement is often a tedious task thus can be easily estimated from proposed infiltration models. The Horton equation is one of the popular models used in the characterization of field infiltration. In this study, the least square curve firing technique was employed to estimate the model parameters from fifteen field measured data and gave resultant mean regression coefficients (R2) value of 0.811. Furthermore, plotting the measured against the calculated infiltration rate for the first six (6) measurement points yielded R2 values close to unity in the regression curve indicating a marked relationship between the two. This indicates that the Horton infiltration model can be applied to estimate infiltration characteristics of soils in Samaru, Northern Guinea Savanna of Nigeria. 展开更多
关键词 Field infiltration Horton's infiltration equation regression least-square technique.
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On Infinitesimal Ⅱ-isometry of Surfaces
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作者 杨纬隆 《Chinese Quarterly Journal of Mathematics》 CSCD 1996年第2期5-10,共6页
In this paper,we try to discuss the conjecture which says that infinitesimal Ⅱ-isome try of surface is infintesimal Ⅰ -isometry. i. e, infinitesimal rigid.Under some weakly suppositions of Guass curvature,some new ... In this paper,we try to discuss the conjecture which says that infinitesimal Ⅱ-isome try of surface is infintesimal Ⅰ -isometry. i. e, infinitesimal rigid.Under some weakly suppositions of Guass curvature,some new results are worked out They are generalizations of known theories. 展开更多
关键词 SURFACE infintesimal Ⅱ-isometry infintesimal rigid
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Evaluation of small bowel blood flow in healthy subjects receiving low-dose aspirin 被引量:3
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作者 Urara Nishida Mototsugu Kato +5 位作者 Mutsumi Nishida Go Kamada Takeshi Yoshida Shouko Ono Yuichi Shimizu Masahiro Asaka 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第2期226-230,共5页
AIM:To investigate the relationship between low-dose aspirin-induced small bowel mucosal damage and blood flow,and the effect of rebamipide. METHODS:Ten healthy volunteers were enrolled in this study.The subjects were... AIM:To investigate the relationship between low-dose aspirin-induced small bowel mucosal damage and blood flow,and the effect of rebamipide. METHODS:Ten healthy volunteers were enrolled in this study.The subjects were divided into two groups:a placebo group given low-dose aspirin plus placebo and a rebamipide group given low-dose aspirin plus rebamipide for a period of 14 d.Capsule endoscopy and contrast-enhanced ultrasonography were performed before and after administration of drugs.Areas under the curves and peak value of time-intensity curve were calculated. RESULTS:Absolute differences in areas under the curves were-1102.5(95%CI:-1980.3 to-224.7,P=0.0194) in the placebo group and-152.7(95%CI:-1604.2 to 641.6,P=0.8172) in the rebamipide group. Peak values of time intensity curves were-148.0(95% CI:-269.4 to-26.2,P=0.0225) in the placebo group and 28.3(95%CI:-269.0 to 325.6,P=0.8343) in the rebamipide group.Capsule endoscopy showed mucosal breaks only in the placebo group. CONCLUSION:Short-term administration of low-dose aspirin is associated with small bowel injuries and blood flow. 展开更多
关键词 Small-intestine Capsule endoscopy Lowdose aspirin Contrast-enhanced ultrasonography REBAMIPIDE
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“Passive-bending colonoscope”significantly improves cecal intubation in difficult cases 被引量:1
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作者 Takeshi Mizukami Haruhiko Ogata Toshihumi Hibi 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第32期4454-4456,共3页
Colonoscopy sometimes causes pain during insertion,especially in difficult cases.Over-insufflation of air causes elongation or acute angulations of the colon,making passage of the scope difficult and causing pain.We p... Colonoscopy sometimes causes pain during insertion,especially in difficult cases.Over-insufflation of air causes elongation or acute angulations of the colon,making passage of the scope difficult and causing pain.We previously reported a sedative-risk-free colonoscopy insertion technique,namely,"Water Navigation Colonoscopy".Complete air suction after water infusion not only improves the vision,but also makes water flow down to the descending colon,while the sigmoid colon collapses and shortens.While non-sedative colonoscopy can be carried out without pain in most cases,some patients do complain of pain.Most of these patients have abnormal colon morphology,and the pain is caused while negotiating the "hairpin" bends of the colon.The "hairpin" bends of the colon should be negotiated by gently pushing the full-angled colonoscope.The proximal 10-20 cm from the angulated part of the conventional colonoscope is stiff,with a wide turning radius,therefore,a conventional colonoscope cannot be negotiated through the "hairpin" bends of the colon without stretching them and causing pain.The "passive-bending colonoscope" has a flexible tip with a narrow turning radius,so that the scope can be negotiated through the "hairpin" bends of the colon with a minimum turning radius and minimal discomfort.Therefore,the intubation and pain-reducing performance of the "passive-bending colonoscope" was assessed in difficult cases. 展开更多
关键词 Computed tomographic colonography Water navigation colonoscopy Passive-bending colonoscope Cecal intubation
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A Theorem on Infinitesimal I-isometry of Surfaces Immersed in a Space with Constant Curvature
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作者 程新跃 杨文茂 邱敦元 《Chinese Quarterly Journal of Mathematics》 CSCD 1997年第4期63-69, ,共7页
In this paper we discuss the infinitesimal I-isometric de formations of surfaces immersed in a space with constant curvature. We obtain a sufficient condition for the de formation vector field to be zero vector field ... In this paper we discuss the infinitesimal I-isometric de formations of surfaces immersed in a space with constant curvature. We obtain a sufficient condition for the de formation vector field to be zero vector field which is generalization of the results in [1] and [2]. 展开更多
关键词 infinitesimal isometric deformation mean curvature vector sectional curvature
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Crack detection using integrated signals from dynamic responses of girder bridges
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作者 王佐才 任伟新 《Journal of Central South University》 SCIE EI CAS 2013年第6期1759-1766,共8页
An innovative approach for the identification of cracks from the dynamic responses of girder bridges was proposed.One of the key steps of the approach was to transform the dynamical responses into the equivalent stati... An innovative approach for the identification of cracks from the dynamic responses of girder bridges was proposed.One of the key steps of the approach was to transform the dynamical responses into the equivalent static quantities by integrating the excitation and response signals over time.A sliding-window least-squares curve fitting technique was then utilized to fit a cubic curve for a short segment of the girder.The moment coefficient of the cubic curve can be used to detect the locations of multiple cracks along a girder bridge.To validate the proposed method,prismatic girder bridges with multiple cracks of various depths were analyzed.Sensitivity analysis was conducted on various effects of crack depth,moving window width,noise level,bridge discretization,and load condition.Numerical results demonstrate that the proposed method can accurately detect cracks in a simply-supported or continuous girder bridges,the five-point equally weighted algorithm is recommended for practical applications,the spacing of two discernable cracks is equal to the window length,and the identified results are insensitive to noise due to integration of the initial data. 展开更多
关键词 crack identification dynamic response equivalent static sliding-window least-squares
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Detection of Aflatoxin from Some Aspergillus sp. Isolated from Wheat Seeds
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作者 Taha Jalal Omar Zrari 《Journal of Life Sciences》 2013年第10期1041-1047,共7页
Ten seed borne fungi (Alternaria sp., Aspergillus sp., Aureobasidium sp., Cladosporium sp., Dreschslera sp., Penicillium sp., Rhizoctonia sp., Stemphylium sp., Mueor sp. and Rhizopus sp.) were isolated and identifie... Ten seed borne fungi (Alternaria sp., Aspergillus sp., Aureobasidium sp., Cladosporium sp., Dreschslera sp., Penicillium sp., Rhizoctonia sp., Stemphylium sp., Mueor sp. and Rhizopus sp.) were isolated and identified from two wheat varieties, the highest frequency of seed borne fungi was observed on wheat cultivar site Moll4 Alternaria sp.. Their mean and standard deviation was (5.5 ~ 1.69) while the lowest frequency fungal isolated was Dreschslera sp. and Rhizopus sp.. Their mean and standard deviation was (0.1 ~ 0.64). The aflatoxin-producing isolates appeared as gray or black colonies in the UV photographs, whereas nonproducing isolates appeared as white colonies, the plate five colony four (P5CO4) showed the positive results which means the presence of aflatoxin as compaired to the control which showed the ngative results. Ammonium Hydroxide Vapor-Induced Color Change method used which the dish was inverted and 1 or 2 drops of concentrated ammonium hydroxide solution are placed on the inside of the lid. The undersides of aflatoxin-producing colonies quickly turn plum-red after the bottom of the Petri dish has been inverted over the lid containing the ammonium hydroxide aspositive result in (P5CO4) and (P7CO4) observed. Essentially no color change occurs on the undersides of colonies that are not producing aflatoxinsthis indicate to the negative results (control). The main objective of this study is to isolation, identification and rapid detection of aflatoxin from wheat seed borne fungi. 展开更多
关键词 AFLATOXIN Apergillus sp. wheat seeds.
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Construction of mTNFR1shRNA Plasmid and its Biological Effects on MHV-3 Induced Fulminant Hepatitis in BALB/cJ Mice 被引量:1
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作者 Sui GAO Ming WANG Jian-wen GUO Dong Xi Xiao-ping LUO Qin NING 《Virologica Sinica》 SCIE CAS CSCD 2010年第1期52-58,共7页
Previous study on TNFRl-mediated hepatocyte apoptosis has been implicated in the development of fulminant viral hepatitis. To interfere with the potentially effective target, plasmid named p-mTNFRlshRNA complimentary ... Previous study on TNFRl-mediated hepatocyte apoptosis has been implicated in the development of fulminant viral hepatitis. To interfere with the potentially effective target, plasmid named p-mTNFRlshRNA complimentary to the sequence responsible for mTNFR1 was also constructed and further confirmed by sequence analysis. To investigate the effect of mTNFRlshRNA plasmid on mTNFR1 expression in vivo and the disease progress in MHV-3 induced fulminant hepatitis mice model. By hydrodynamic injection of mTNFRlshRNA plasmid, the survival rate of mice, hepatic pathological change were examined and compared between mice with/without mTNFRlshRNA plasmid intervention. The expression of mTNFR1 was detected by Real-time PCR, immunohistochemistry staining. The mTNFRlshRNA plasmid significantly reduced mTNFR1 expression in vivo, markedly ameliorates inflammatory infiltration, prolonged the survival time period and elevated the survival rate from 0 up to 13.3% in Balb/cJ mice with MHV-3 induced fulminant hepatitis. This study was designed to explore the opportunity of RNA interference technique in inhibiting TNFR1 expression, which has been reported to be involved in the development of a variety of diseases including fulminant viral hepatitis and severe chronic hepatitis B. 展开更多
关键词 Fulminant hepatitis TNFR1 RNA interference SHRNA
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