The influence of prebiotics as lactulose as well inulin on the ability of Bifidobacterium lactis to reduce cholesterol in milk was studied during milk fermentation. Pasteurized milk, freeze-dried starter culture Bb-12...The influence of prebiotics as lactulose as well inulin on the ability of Bifidobacterium lactis to reduce cholesterol in milk was studied during milk fermentation. Pasteurized milk, freeze-dried starter culture Bb-12 （Bifidobacterium lactis, Chr. Hansen, Denmark）, inulin--RAFTILINEHP （ORAFI, Belgium）, syrup of lactulose （Duphalac, the Netherlands） in following concentrations： 0, 1%, 2%, 3%, 4% and 5% were used for experiments. The fermentation process was realized at 37 ℃ for 16 h. The content of cholesterol was determined according to AOAC Official Method 976.26A. The results showed that it is possible considerable to lower the level of cholesterol in fermented milk using B. lactis. The ability of B. lactis to decrease the level of cholesterol in milk can be influenced with addition of prebiotics. The lower concentration of cholesterol was determined in fermented samples with 4% of lactulose （9.5 mg/100g） and with 1% of inulin （10.4 mg/100g）. Evaluating the influence of prebiotics on cholesterol content in fermented milk samples, it is obvious that the influence depends on the type of prebiotics （P 〉 0.05） and their concentrations （P 〈 0.05）.展开更多
The ingestion of probiotic lactic acid bacteria has been evaluated and noted that it has an effect on the balance of desirable microbiota in the gastrointestinal tract. Lactobacillus gasseri demonstrates good survival...The ingestion of probiotic lactic acid bacteria has been evaluated and noted that it has an effect on the balance of desirable microbiota in the gastrointestinal tract. Lactobacillus gasseri demonstrates good survival in the gastrointestinal tract, and it has been associated with a variety of probiotic activities and roles, including the reduction of fecal mutagenic enzymes, the production of bacteriocins and the stimulation of macrophages immunomodulation. The aim of the study was to evaluate the effects of a pool of L. gasseri strains isolated from the feces of breastfed infants added in the human milk of healthy women. The milk was both pasteurized and unpasteurized, to verify the cell cytotoxicity of macrophages and to quantify the production of immunologic mediators such as IL-4, IL-6, IFN-γ, TNF-α, NO and oxygen intermediary compounds （H2O2）. The administration of raw human milk and pasteurized human milk to infants is a regular, encouraged practice in units of intensive therapy （UITs） and our present investigation verified the beneficial effect of addition of a pool of L. gasseri to pasteurized human milk （PHML）. Our results show that probiotic supplementation helped to maintain cell viability, reduced IL-6 and IFN-γ production and stimulated TNF-α, NO, H2O2, IL-4 production. Nevertheless, the results indicate that the addition of lactobacillus to human milk was not a determinant in the production of TNF-α. L. gasseri added to breast milk did not present a cytotoxic risk, and the addition ofL. gasseri to pasteurized milk of human milk bank would benefit newborns that depend on milk banks for the colonization of more desirable microbiota.展开更多
A total of 57 samples, of which 17 were surface water samples and 40 were dairy food samples （raw milk, pasteurized milk, icecream, sweet, milk based drink like matha and borhani） were tested for the isolation ofLis...A total of 57 samples, of which 17 were surface water samples and 40 were dairy food samples （raw milk, pasteurized milk, icecream, sweet, milk based drink like matha and borhani） were tested for the isolation ofListeria spp. Putative Listeria isolates were identified by conventional microbiological tests and Analytical Profile Index. Overall prevalence ofListeria spp. in both food and water samples were 8.77%, of which one was （1.75%） Listeria monocytogenes, 2 （3.5%） were Listeria innocua and 2 were （3.5%） Listeria welshimeri. When compared between two types of samples, water samples contained two Listeria spp. （11.76%） of which one was pathogenic Listeria monocytogenes and the other was Listeria innocua. In case of food samples, three Listeria spp. （7.5%） were isolated of which one was Listeria innocua （icecream sample） and two were Listeria welshimeri （icecream sample and raw milk）. No Listeria was found in pasteurized milk, sweet, matha and borhani. Antibiotic resistance profile of the Listeria isolates showed that 60% isolates were resistant to Ampicillin and Erythromycin, 20% isolates were Sulphamethoxazole and Ciprofloxacin resistant. No resistance was observed to Chloramphenicol for any Listeria isolates.展开更多
文摘The influence of prebiotics as lactulose as well inulin on the ability of Bifidobacterium lactis to reduce cholesterol in milk was studied during milk fermentation. Pasteurized milk, freeze-dried starter culture Bb-12 （Bifidobacterium lactis, Chr. Hansen, Denmark）, inulin--RAFTILINEHP （ORAFI, Belgium）, syrup of lactulose （Duphalac, the Netherlands） in following concentrations： 0, 1%, 2%, 3%, 4% and 5% were used for experiments. The fermentation process was realized at 37 ℃ for 16 h. The content of cholesterol was determined according to AOAC Official Method 976.26A. The results showed that it is possible considerable to lower the level of cholesterol in fermented milk using B. lactis. The ability of B. lactis to decrease the level of cholesterol in milk can be influenced with addition of prebiotics. The lower concentration of cholesterol was determined in fermented samples with 4% of lactulose （9.5 mg/100g） and with 1% of inulin （10.4 mg/100g）. Evaluating the influence of prebiotics on cholesterol content in fermented milk samples, it is obvious that the influence depends on the type of prebiotics （P 〉 0.05） and their concentrations （P 〈 0.05）.
文摘The ingestion of probiotic lactic acid bacteria has been evaluated and noted that it has an effect on the balance of desirable microbiota in the gastrointestinal tract. Lactobacillus gasseri demonstrates good survival in the gastrointestinal tract, and it has been associated with a variety of probiotic activities and roles, including the reduction of fecal mutagenic enzymes, the production of bacteriocins and the stimulation of macrophages immunomodulation. The aim of the study was to evaluate the effects of a pool of L. gasseri strains isolated from the feces of breastfed infants added in the human milk of healthy women. The milk was both pasteurized and unpasteurized, to verify the cell cytotoxicity of macrophages and to quantify the production of immunologic mediators such as IL-4, IL-6, IFN-γ, TNF-α, NO and oxygen intermediary compounds （H2O2）. The administration of raw human milk and pasteurized human milk to infants is a regular, encouraged practice in units of intensive therapy （UITs） and our present investigation verified the beneficial effect of addition of a pool of L. gasseri to pasteurized human milk （PHML）. Our results show that probiotic supplementation helped to maintain cell viability, reduced IL-6 and IFN-γ production and stimulated TNF-α, NO, H2O2, IL-4 production. Nevertheless, the results indicate that the addition of lactobacillus to human milk was not a determinant in the production of TNF-α. L. gasseri added to breast milk did not present a cytotoxic risk, and the addition ofL. gasseri to pasteurized milk of human milk bank would benefit newborns that depend on milk banks for the colonization of more desirable microbiota.
文摘A total of 57 samples, of which 17 were surface water samples and 40 were dairy food samples （raw milk, pasteurized milk, icecream, sweet, milk based drink like matha and borhani） were tested for the isolation ofListeria spp. Putative Listeria isolates were identified by conventional microbiological tests and Analytical Profile Index. Overall prevalence ofListeria spp. in both food and water samples were 8.77%, of which one was （1.75%） Listeria monocytogenes, 2 （3.5%） were Listeria innocua and 2 were （3.5%） Listeria welshimeri. When compared between two types of samples, water samples contained two Listeria spp. （11.76%） of which one was pathogenic Listeria monocytogenes and the other was Listeria innocua. In case of food samples, three Listeria spp. （7.5%） were isolated of which one was Listeria innocua （icecream sample） and two were Listeria welshimeri （icecream sample and raw milk）. No Listeria was found in pasteurized milk, sweet, matha and borhani. Antibiotic resistance profile of the Listeria isolates showed that 60% isolates were resistant to Ampicillin and Erythromycin, 20% isolates were Sulphamethoxazole and Ciprofloxacin resistant. No resistance was observed to Chloramphenicol for any Listeria isolates.
