A chimeric gene, Bt29K, composed of coding sequences of activated Cry1Ac insecticidal protein and an endoplasm reticulum-retarding signal peptide, was synthesized. A plant expression vector containing two expression c...A chimeric gene, Bt29K, composed of coding sequences of activated Cry1Ac insecticidal protein and an endoplasm reticulum-retarding signal peptide, was synthesized. A plant expression vector containing two expression cassettes for the Bt29K and API-B genes was constructed. These two insect-resistant genes were transferred into two cotton ( Gossypium hirsutum L.) varieties ( or lines) via Agrobacterium-mediated transformation and nine homozygous transgenic cotton lines showing a mortality of 90.0% - 99.7% to cotton ballworm (Heliothis armigera) larvae and good agronomic traits were selected through six generations. Molecular biology analysis revealed that one or two copies of the insecticidal protein genes were integrated into the transgenic cotton genome and activated Cry1Ac and API-B protein expression was at a level of 0.17% and 0.09% of the total soluble protein in the transgenic cotton leaves, respectively. Comparison of the insect-resistance of the homozygous lines expressing the activated chimeric Cry1Ac and API-B with that expressing Cry1Ac only revealed that the insect-resistance of the former is apparently higher than the latter. These results also indicate that the strategy to construct a plant expression vector expressing two different insect-resistant genes reported here is reasonable.展开更多
[Objective] This study was to optimize the experimental conditions for large scale propagation of Sagittaria trifolia L via tissue culture.[Method] The dominant S.trifolia cultivar Baoyingziyuan introduced from Jiangs...[Objective] This study was to optimize the experimental conditions for large scale propagation of Sagittaria trifolia L via tissue culture.[Method] The dominant S.trifolia cultivar Baoyingziyuan introduced from Jiangsu Province was used as experimental material to study the impacts of various culture conditions on tissue culture of its stem tips and induction of stolons.[Result] Hormone combination 0.10 mg/L 6-BA ＋0.05 mg/L NAA performed best in plantlet regeneration and 2.0 mg/L 6-BA ＋0.5 mg/L NAA best in induction of stolon.Various sucrose concentrations did not show significant difference in the impact on sprouted stolons.[Conclusion] Various culture conditions could to some extent impact plantlet regeneration and stolon induction,and our results reveal the optimal hormone combinations for regeneration and stolon induction of S.trifolia.展开更多
[Objective] The aim was to explore the factors affecting the herbicidal activity of pyrazosulfuron-ethyl against Sagittaria pygmaea in paddy soil.[Method] The bioassay method was carried out under greenhouse condition...[Objective] The aim was to explore the factors affecting the herbicidal activity of pyrazosulfuron-ethyl against Sagittaria pygmaea in paddy soil.[Method] The bioassay method was carried out under greenhouse conditions.[Result] The reduction of more than 80% in fresh weight was observed in S.pygmaea treated with 45 g/hm2 of pyrazosulfuron-ethyl for 7 d or more.Application of 60 g/hm2 at the sprouting stage of S.pygmaea suppressed completely the growth in fresh weight irrespective of tuber size.However,at 6 g/hm2 growth of the weed sustained less reduction with increasing tuber size.Differences in flooding water depth and temperature had no appreciable influence on the herbicidal activity.Growth inhibition of S.pygmaea by pre-emergence or 1.5-leaf stage applications under non-leaching conditions was significantly higher than that under water leaching conditions.Under non-leaching condition,there was no remarkable difference in this inhibition between preemergence and 1.5-leaf stage applications,while under water leaching conditions the growth reduction with the latter application was significantly larger.Growth inhibition of S.pygmaea planted at a depth of 2 cm was significantly higher than that planted deeper （5 cm） with the pre-emergence applications.[Conclusion] This study had provide basis for the further study on the inhibition effect of pyrazosulfuron-ethyl.展开更多
[Objective] To screen the optimal fertilizer formula, and to lay foundation for the research on special fertilizer for Sagittaria sagittifolia production. [Method] Based on the ＂3414＂ fertilization design, the yield...[Objective] To screen the optimal fertilizer formula, and to lay foundation for the research on special fertilizer for Sagittaria sagittifolia production. [Method] Based on the ＂3414＂ fertilization design, the yield of S. sagittifolia in each treatment was determined, and the effects of N, P and K treatments on the yield and eco- nomic benefit of S. sagittifolia were investigated, obtaining the optimal fertilizer appli- cation ratio. [Result] N, P and K had relatively good effects on the yield of S. sagittifolia. Treatment of N2P2K3 showed the optimal fertilizer application ratio, with the yield of 18 397.80 kg/hm2, yield increasing rate of 104.77%, and net income in- crease of 80 317.35 Yuan/hm2. N fertilizer had the greatest impacts on the yield of S. sagittifolia, followed with K fertilizer; while P fertilizer showed the poorest impacts. [Conclusion] To achieve the highest yield of S. sagittifolia, the fertilizer application was as follows： N of 446.70 kg/hm2, P20 of 568.10 kg/hm2, K2O of 534.90 kg/hm2, when the highest yield was 18 612.75 kg/hm2. Fertilizer application for the optimal economic benefit was N of 420.60 kg/hm2, P2O5 of 70.50 kg/hm2, K2O of 492.15 kg/hm2, when the optimal yield was 18 579.00 kg/hm2.展开更多
[Objective] This experiment was conducted to investigate the optimal ex- traction process and antioxidant activity of polysaccharide in Sagittaria sagittifo/ia L. [Method] The effects of extraction temperature, extrac...[Objective] This experiment was conducted to investigate the optimal ex- traction process and antioxidant activity of polysaccharide in Sagittaria sagittifo/ia L. [Method] The effects of extraction temperature, extraction time, extraction times and material-to-liquid ratio on content of polysaccharides in S. sagittifo/ia were investi- gated. On the basis of single-factor tests, an orthogonal test was carried out to de- termine the optimal process parameters. In addition, the in-vitro antioxidant activity of S. sagittifo/ia polysaccharides was evaluated on the basis of determination of their scavenging activities against DPPH and hydroxyl free radicals and reducing power. [Result] The results showed that the optimized process parameters were as follows： material-to-liquid ratio at 1：40 （g/ml）, extraction temperature at 90 ℃, extrac- tion time of 4 h, and extraction times of 3 times. Under this condition, the content of S. sagittifo/ia polysaccharides was 29.32%. For 1 mg/ml S. sagittifo/ia polysaccha- rides, the scavenging ratios of DPPH and hydroxyl free radicals were 70.62% and 35.82%, respectively, and in the determination of reducing power, the absorbance at 700 nm was 0.453 1. [Conclusion] It could be concluded that S. sagittifolia polysac- charides have strong antioxidant capacity to have free radicals scavenged in vitro.展开更多
Social capital has played an increasingly important role in regional development.China is a country with high stocks of social capital.Using several different indicators of social capital,this study tries to research ...Social capital has played an increasingly important role in regional development.China is a country with high stocks of social capital.Using several different indicators of social capital,this study tries to research the regional disparities in social capital and the influence of social capital on economic growth of China in 1978-2004.Measuring social capital with indicators of associations,charities and blood donation rates,this study finds significant regional disparities in social capital at provincial level in China.Those indicators for social capital are highly correlated with regional economic performance.Statistical analysis shows that social capital has a significant and positive effect on a long-term provincial economic growth.This relationship exists after controlling policy,macro location factors,and per capita GDP in the initial year.The empirical findings indicate that institutions,culture and social relations are critical for regional development in China.Therefore,the creation and support of social capital should be paid more attention to when making regional policy.展开更多
[Objective] This study was to study the optimal extraction technology and anti-inflammatory effects of colchicine from Sagittaria sagittifolia. [Method] The ef- fects of ethanol concentration, extraction time, extract...[Objective] This study was to study the optimal extraction technology and anti-inflammatory effects of colchicine from Sagittaria sagittifolia. [Method] The ef- fects of ethanol concentration, extraction time, extraction temperature and solid-liquid ratio on the extraction rate of colchicine from S. sagittifolia were investigated. On the basis of single-factor experiments, an L9 （34） orthogonal test was carried out to optimize the extraction process. According to the optimal extraction process, the content of colchicine in S. sagittifolia was determined by high performance liquid chromatography. The anti-inflammatory ability of colchicine was evaluated through an anti-inflammatory test in vitro. [Result] The optimal extraction process of colchicine from S. sagittifolia was as follows： ethanol concentration of 60%, extraction temper- ature of 50℃, extraction time of 30 min, and solid-liquid ratio of 1：25 （g/ml）. The content of colchicine in S. sagittifolia was determined as 40.58 μg/100 mg. Com- pared with the control, the colchicine from S. sagittifolia （9.0 and 4.5 g/kg） inhibited the increase in PGE2, TNF-α and IL-1β contents in pleural fluid （P〈0.05）. High-dose colchicine inhibited the increase in TNF-α, IL-1β and MDA contents in lung tissue （P〈0.01）, and middle-dose colchicine inhibited the increase in IL-1β content in lung tissue （P〈0.01）. [Conclusion] The colchicine in S. sagittifolia has a good anti-inflam- matory effect, which may be achieved through hindering the production of inflam- matory mediators and antioxidation.展开更多
Non-profit organizations （NPOs） in Malaysia are not subjected to statutory requirement to follow the accounting standards when preparing their annual reports. This study analyzed the annual reports of 100 NPOs regis...Non-profit organizations （NPOs） in Malaysia are not subjected to statutory requirement to follow the accounting standards when preparing their annual reports. This study analyzed the annual reports of 100 NPOs registered with the Registry of Society （ROS） under the charity and religious categories. The objectives of this study are： （1） to determine the current state of reporting practices by NPOs; and （2） to describe the contents of a set of annual reports and the financial statement presentation of NPOs. This study found that there were variations in reporting practices among the charity and religious categories of NPOs. On average, the results show that NPOs do not focus on the disclosing information required by the ROS, the governing body of NPOs. This indicates that the regulators need to strongly practice enforcement to strengthen the compliance of the annual reports submission. Overall, the findings of this study provide useful information to regulators as a basis for preparing and presenting NPOs＇ annual report framework in the future.展开更多
文摘A chimeric gene, Bt29K, composed of coding sequences of activated Cry1Ac insecticidal protein and an endoplasm reticulum-retarding signal peptide, was synthesized. A plant expression vector containing two expression cassettes for the Bt29K and API-B genes was constructed. These two insect-resistant genes were transferred into two cotton ( Gossypium hirsutum L.) varieties ( or lines) via Agrobacterium-mediated transformation and nine homozygous transgenic cotton lines showing a mortality of 90.0% - 99.7% to cotton ballworm (Heliothis armigera) larvae and good agronomic traits were selected through six generations. Molecular biology analysis revealed that one or two copies of the insecticidal protein genes were integrated into the transgenic cotton genome and activated Cry1Ac and API-B protein expression was at a level of 0.17% and 0.09% of the total soluble protein in the transgenic cotton leaves, respectively. Comparison of the insect-resistance of the homozygous lines expressing the activated chimeric Cry1Ac and API-B with that expressing Cry1Ac only revealed that the insect-resistance of the former is apparently higher than the latter. These results also indicate that the strategy to construct a plant expression vector expressing two different insect-resistant genes reported here is reasonable.
基金Supported by Science and Technology Foundation of Guizhou Province(20092108)Key Agricultural Program from Science and Technology Bureau of Guiyang City(201001)Science Research Program for Doctors in Guizhou Normal University(2008)~~
文摘[Objective] This study was to optimize the experimental conditions for large scale propagation of Sagittaria trifolia L via tissue culture.[Method] The dominant S.trifolia cultivar Baoyingziyuan introduced from Jiangsu Province was used as experimental material to study the impacts of various culture conditions on tissue culture of its stem tips and induction of stolons.[Result] Hormone combination 0.10 mg/L 6-BA ＋0.05 mg/L NAA performed best in plantlet regeneration and 2.0 mg/L 6-BA ＋0.5 mg/L NAA best in induction of stolon.Various sucrose concentrations did not show significant difference in the impact on sprouted stolons.[Conclusion] Various culture conditions could to some extent impact plantlet regeneration and stolon induction,and our results reveal the optimal hormone combinations for regeneration and stolon induction of S.trifolia.
基金Supported by the Science and Technology Innovation Platform Construction Project of Institution of Higher Education in Hunan Province(XCJZ2009-070)the Earmarked Fund for Mordern Agro-industry Technology Research System(CARS-19-E12)~~
文摘[Objective] The aim was to explore the factors affecting the herbicidal activity of pyrazosulfuron-ethyl against Sagittaria pygmaea in paddy soil.[Method] The bioassay method was carried out under greenhouse conditions.[Result] The reduction of more than 80% in fresh weight was observed in S.pygmaea treated with 45 g/hm2 of pyrazosulfuron-ethyl for 7 d or more.Application of 60 g/hm2 at the sprouting stage of S.pygmaea suppressed completely the growth in fresh weight irrespective of tuber size.However,at 6 g/hm2 growth of the weed sustained less reduction with increasing tuber size.Differences in flooding water depth and temperature had no appreciable influence on the herbicidal activity.Growth inhibition of S.pygmaea by pre-emergence or 1.5-leaf stage applications under non-leaching conditions was significantly higher than that under water leaching conditions.Under non-leaching condition,there was no remarkable difference in this inhibition between preemergence and 1.5-leaf stage applications,while under water leaching conditions the growth reduction with the latter application was significantly larger.Growth inhibition of S.pygmaea planted at a depth of 2 cm was significantly higher than that planted deeper （5 cm） with the pre-emergence applications.[Conclusion] This study had provide basis for the further study on the inhibition effect of pyrazosulfuron-ethyl.
基金Supported by the Test Station Program of Sagittaria sagittifolia as the Feature Crop of Pingle,Guangxi Province(Gui TS201409)~~
文摘[Objective] To screen the optimal fertilizer formula, and to lay foundation for the research on special fertilizer for Sagittaria sagittifolia production. [Method] Based on the ＂3414＂ fertilization design, the yield of S. sagittifolia in each treatment was determined, and the effects of N, P and K treatments on the yield and eco- nomic benefit of S. sagittifolia were investigated, obtaining the optimal fertilizer appli- cation ratio. [Result] N, P and K had relatively good effects on the yield of S. sagittifolia. Treatment of N2P2K3 showed the optimal fertilizer application ratio, with the yield of 18 397.80 kg/hm2, yield increasing rate of 104.77%, and net income in- crease of 80 317.35 Yuan/hm2. N fertilizer had the greatest impacts on the yield of S. sagittifolia, followed with K fertilizer; while P fertilizer showed the poorest impacts. [Conclusion] To achieve the highest yield of S. sagittifolia, the fertilizer application was as follows： N of 446.70 kg/hm2, P20 of 568.10 kg/hm2, K2O of 534.90 kg/hm2, when the highest yield was 18 612.75 kg/hm2. Fertilizer application for the optimal economic benefit was N of 420.60 kg/hm2, P2O5 of 70.50 kg/hm2, K2O of 492.15 kg/hm2, when the optimal yield was 18 579.00 kg/hm2.
基金Supported by Scientific Research Project of Education Department of Sichuan Province(16ZB0199)~~
文摘[Objective] This experiment was conducted to investigate the optimal ex- traction process and antioxidant activity of polysaccharide in Sagittaria sagittifo/ia L. [Method] The effects of extraction temperature, extraction time, extraction times and material-to-liquid ratio on content of polysaccharides in S. sagittifo/ia were investi- gated. On the basis of single-factor tests, an orthogonal test was carried out to de- termine the optimal process parameters. In addition, the in-vitro antioxidant activity of S. sagittifo/ia polysaccharides was evaluated on the basis of determination of their scavenging activities against DPPH and hydroxyl free radicals and reducing power. [Result] The results showed that the optimized process parameters were as follows： material-to-liquid ratio at 1：40 （g/ml）, extraction temperature at 90 ℃, extrac- tion time of 4 h, and extraction times of 3 times. Under this condition, the content of S. sagittifo/ia polysaccharides was 29.32%. For 1 mg/ml S. sagittifo/ia polysaccha- rides, the scavenging ratios of DPPH and hydroxyl free radicals were 70.62% and 35.82%, respectively, and in the determination of reducing power, the absorbance at 700 nm was 0.453 1. [Conclusion] It could be concluded that S. sagittifolia polysac- charides have strong antioxidant capacity to have free radicals scavenged in vitro.
基金Under the auspices of National Natural Science Foundation of China(No.40871065,40830747)
文摘Social capital has played an increasingly important role in regional development.China is a country with high stocks of social capital.Using several different indicators of social capital,this study tries to research the regional disparities in social capital and the influence of social capital on economic growth of China in 1978-2004.Measuring social capital with indicators of associations,charities and blood donation rates,this study finds significant regional disparities in social capital at provincial level in China.Those indicators for social capital are highly correlated with regional economic performance.Statistical analysis shows that social capital has a significant and positive effect on a long-term provincial economic growth.This relationship exists after controlling policy,macro location factors,and per capita GDP in the initial year.The empirical findings indicate that institutions,culture and social relations are critical for regional development in China.Therefore,the creation and support of social capital should be paid more attention to when making regional policy.
基金Supported by Scientific Research Foundation of the Education Department of Sichuan Province,China~~
文摘[Objective] This study was to study the optimal extraction technology and anti-inflammatory effects of colchicine from Sagittaria sagittifolia. [Method] The ef- fects of ethanol concentration, extraction time, extraction temperature and solid-liquid ratio on the extraction rate of colchicine from S. sagittifolia were investigated. On the basis of single-factor experiments, an L9 （34） orthogonal test was carried out to optimize the extraction process. According to the optimal extraction process, the content of colchicine in S. sagittifolia was determined by high performance liquid chromatography. The anti-inflammatory ability of colchicine was evaluated through an anti-inflammatory test in vitro. [Result] The optimal extraction process of colchicine from S. sagittifolia was as follows： ethanol concentration of 60%, extraction temper- ature of 50℃, extraction time of 30 min, and solid-liquid ratio of 1：25 （g/ml）. The content of colchicine in S. sagittifolia was determined as 40.58 μg/100 mg. Com- pared with the control, the colchicine from S. sagittifolia （9.0 and 4.5 g/kg） inhibited the increase in PGE2, TNF-α and IL-1β contents in pleural fluid （P〈0.05）. High-dose colchicine inhibited the increase in TNF-α, IL-1β and MDA contents in lung tissue （P〈0.01）, and middle-dose colchicine inhibited the increase in IL-1β content in lung tissue （P〈0.01）. [Conclusion] The colchicine in S. sagittifolia has a good anti-inflam- matory effect, which may be achieved through hindering the production of inflam- matory mediators and antioxidation.
文摘Non-profit organizations （NPOs） in Malaysia are not subjected to statutory requirement to follow the accounting standards when preparing their annual reports. This study analyzed the annual reports of 100 NPOs registered with the Registry of Society （ROS） under the charity and religious categories. The objectives of this study are： （1） to determine the current state of reporting practices by NPOs; and （2） to describe the contents of a set of annual reports and the financial statement presentation of NPOs. This study found that there were variations in reporting practices among the charity and religious categories of NPOs. On average, the results show that NPOs do not focus on the disclosing information required by the ROS, the governing body of NPOs. This indicates that the regulators need to strongly practice enforcement to strengthen the compliance of the annual reports submission. Overall, the findings of this study provide useful information to regulators as a basis for preparing and presenting NPOs＇ annual report framework in the future.
