This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has bee...This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has been made by Chinese in nearly every branches of tissue culture, including in vitro organogenesis, shoot tip culture, anther culture, ovary culture, endosperm culture, protoplast culture as well as mass cell culture. On the basis of reviewing the articles written by Chinese on plant tissue culture, the internationally recognized contributions are specially mentioned. The applications of plant tissue culture to agriculture and industry in China are also introduced.展开更多
[Objective] This study was to investigate the in vitro culture of unfertilized ovary of strawberry.[Methods] Employing single factor experiment,we investigated six key factors including genotype,extrinsic hormone,sucr...[Objective] This study was to investigate the in vitro culture of unfertilized ovary of strawberry.[Methods] Employing single factor experiment,we investigated six key factors including genotype,extrinsic hormone,sucrose concentration,low temperature pretreatment,growth environment and development status,and illumination condition on induction of gynogenesis in vitro of unfertilized ovary,on the induction of gynogenesis in vitro of unfertilized ovary.[Results] The optimal conditions for in vitro culture of unfertilized ovary of strawberry were as follows:the primary flower buds cultured on bare land as explants,selection of appropriate genotype,2,4-D as external hormone,sucrose at the concentration of 6%,low temperature pretreatment for 48 hours and dark culture under alternated temperature.[Conclusion] The research provided reference for ploidy breeding in strawberry.展开更多
Anomozamites haifanggouensis (Kimura et al.) Zheng et Zhang comb. nov. is a characteristic Bennettitalean plant in Middle Jurassic flora. Only one specimen was collected from Haifanggou Formation (Middle Jurassic) in ...Anomozamites haifanggouensis (Kimura et al.) Zheng et Zhang comb. nov. is a characteristic Bennettitalean plant in Middle Jurassic flora. Only one specimen was collected from Haifanggou Formation (Middle Jurassic) in Daohugou village, Shantou town, Ningcheng County, Nei Mongol Autonomous Region, China. The stem of plant is slender and repeatedly branches as a dichasial system with a fertile shoot in the fork, forming equal and widely divergent branches, on the branches born with the fronds of Anomozamites type. Its reproductive organs consists of some microsporophylls and a few of the bracteoid small leaves (or receptacular leaves). Some scattered bracteoid leaves and microsporophylls were formerly considered by Pan as dicots. Since then, they were proposed to be a non-committal genus Pankuangia and described them as P. haifanggouensis by Kimura et al. In this study, we discover that these bracteoid small leaves and microsporophylls should be of Anomozamites. This discovery provides strong evidence for the 'Pankuangia' determination of the taxonomical position.展开更多
AIM To investigate the role of regulatory T cell(Treg) subsets in the balance between Treg and T helper 17(Th17) cells in various tissues from mice with dextran sulfate sodium-induced colitis.METHODS T r e g c e l l s...AIM To investigate the role of regulatory T cell(Treg) subsets in the balance between Treg and T helper 17(Th17) cells in various tissues from mice with dextran sulfate sodium-induced colitis.METHODS T r e g c e l l s, T r e g c e l l s u b s e t s, T h 1 7 c e l l s, a n d CD4+CD25+FoxP 3+IL-17+ cells from the lamina propria of colon(LPC) and other ulcerative colitis(UC) mouse tissues were evaluated by flow cytometry. Forkhead box protein 3(FoxP 3), interleukin 17A(IL-17A), and RORC m RNA levels were assessed by real-time PCR, while interleukin-10(IL-10) and IL-17 A levels were detected with a Cytometric Beads Array.RESULTS In peripheral blood monocytes(PBMC), mesenteric lymphnode(MLN), lamina propria of jejunum(LPJ) and LPC from UC mice, Treg cell numbers were increased(P < 0.05), and FoxP 3 and IL-10 mR NA levels were decreased. Th17 cell numbers were also increased in PBMC and LPC, as were IL-17 A levels in PBMC, LPJ, and serum. The number of FrI subset cells(CD4+CD45RA+FoxP 3low) was increased in the spleen, MLN, LPJ, and LPC. FrI I subset cells(CD4+CD45RA-Fox P3high) were decreased among PBMC, MLN, LPJ, and LPC, but the number of Fr III cells(CD4+CD45RA-FoxP 3low) and CD4+CD25+FoxP 3+IL-17A+ cells was increased. Fox P3 m RNA levels in CD4+CD45RA-Fox P3 low cells decreased in PBMC, MLN, LPJ, and LPC in UC mice, while IL-17 A and RORC mR NA increased. In UC mice the distribution of Treg, Th17 cells, CD4+CD45RA-FoxP 3high, and CD4+CD45RA-FoxP 3low cells was higher in LPC relative to other tissues.CONCLUSION Increased numbers of CD4+CD45RA-FoxP 3low cells may cause an imbalance between Treg and Th17 cells that is mainly localized to the LPC rather than secondary lymphoid tissues.展开更多
AIM:To assess the peripheral T lymphocyte subsets in chronic hepatitis B virus(HBV) infection,and their dynamics in response to adefovir dipivoxil monotherapy.METHODS:Proportions and absolute counts of peripheral natu...AIM:To assess the peripheral T lymphocyte subsets in chronic hepatitis B virus(HBV) infection,and their dynamics in response to adefovir dipivoxil monotherapy.METHODS:Proportions and absolute counts of peripheral natural killer cells,B cells,CD8+,CD4+,CD8+ CD38+,CD8+CD28+ and CD4+CD28+ T cells were determined using three-color flow cytometry in chronic hepatitis B patients(n = 35),HBV carriers(n = 25) and healthy controls(n = 35).Adefovir dipivoxil was initiated in 17 chronic hepatitis B patients who were regularly followed for 72 wk,during which period the T cell subsets and serum viral load were measured at each follow-up point.RESULTS:The peripheral CD4+ T cell counts and CD8+ T cell counts decreased in chronic HBV infection.In chronic hepatitis B patients,proportions of CD8+CD38+ T cells were 62.0% ± 14.7%,much higher than those of HBV carriers and healthy con-trols.In the 13 hepatitis B patients who were treated and responded to adefovir dipivoxil,proportions of CD8+CD38+ T cells decreased from 53.9% ± 18.4% pre-therapy to 20.1% ± 11.3% by week 72(P < 0.001),concomitant with viral load decline(HBV DNA fell from 7.31 to 3 log copies/mL).CD8+ T cell counts also underwent an average increase of 218 cells/μL by the end of 72-wk treatment.In those who failed the therapy,the CD8+CD38+ T cell population had more fluctuations.CONCLUSION:CD8+ T cells abnormally activated in chronic HBV infection can be partially reversed by antiviral therapy.HBV-associated immune activation may be a crucial part of the pathogenesis and a promising target of treatment.展开更多
AIM to investigate the expression of proliferating cell nuclear antigen(p CNA)and E-cadherin in gastric carcinoma and to analyze their clinical significance.METHODS A total of 146 patients were selected for this study...AIM to investigate the expression of proliferating cell nuclear antigen(p CNA)and E-cadherin in gastric carcinoma and to analyze their clinical significance.METHODS A total of 146 patients were selected for this study,including 38 patients with intestinal metaplasia,42with dysplasia,and 66 with primary gastric cancer.In addition,40 patients with normal gastric tissues were selected as controls.the expression of p CNA and E-cadherin was detected by immunohistochemistry.Differences in p CNA and the E-cadherin labeling indexes among normal gastric mucosa,intestinal metaplasia,dysplasia,and gastric carcinoma were compared.Subjects with normal gastric tissues were assigned to a normal group,while gastric cancer patients were assigned to a gastric cancer group.the difference in p CNA and E-cadherin expression between these two groups was compared.the relationship between expression of p CNA and E-cadherin and clinicopathological features was also explored in gastric cancer patients.furthermore,prognosis-related factors,as well as the expression of p CNA and E-cadherin,were analyzed in patients with gastric cancer to determine the 3-year survival of these patients.RESULTS the difference in p CNA and the E-cadherin labeling indexes among normal gastric mucosa,intestinal metaplasia,dysplasia,and gastric carcinoma was statistically significant(p<0.05).During the transition of normal gastric mucosa to gastric cancer,the p CNA labeling index gradually increased,while the E-cadherin labeling index gradually decreased(p<0.05).the p CNA labeling index was significantly higher and the E-cadherin labeling index was significantly lower in gastric cancer than in dysplasia(p<0.05).the expression of p CNA was significantly higher in the gastric cancer group than in the normal group,but E-cadherin was weaker(p<0.05).there was a negative correlation between the expression of p CNA and E-cadherin in gastric carcinoma(r=-0.741,p=0.000).p CNA expression differed significantly between gastric cancer patients with and without lymph node metastasis and between patients at different t stages.E-cadherin expression also differed significantly between gastric cancer patients with and without lymph node metastasis(p<0.05).High t stage and positive p CNA expression were risk factors for the prognosis of patients with gastric cancer(RR>1),while the positive expression of E-cadherin was a protective factor(RR<1).the sensitivity,specificity,and accuracy of p CNA positivity in predicting the 3-year survival of patients with gastric cancer were 93.33%,38.89%,and0.64,respectively;while these values for E-cadherin negativity were 80.0%,41.67%,and 0.59,respectively.When p CNA positivity and E-cadherin negativity were combined,the sensitivity,specificity,and accuracy were66.67%,66.67%,and 0.67,respectively.CONCLUSION Combined detection of p CNA and E-cadherin can improve the accuracy of assessing the prognosis of patients with gastric cancer.展开更多
Electric product house of magnesium alloy sheet is usually obtained by warm stamping owing to its poor plasticity and formability at room temperature.The formability of AZ31B magnesium alloy sheet can be improved by r...Electric product house of magnesium alloy sheet is usually obtained by warm stamping owing to its poor plasticity and formability at room temperature.The formability of AZ31B magnesium alloy sheet can be improved by repeated unidirectional bending(RUB)process through control of(0002)basal texture.Compared with as-received sheet,the Erichsen value(IE)of the sheet underwent RUB process increases to 5.90 from 3.53 at room temperature.It is also confirmed that cell phone houses could be stamped successfully in crank press with AZ31B magnesium alloy sheets underwent RUB process.It provides an alternative to the electronics industry in the application of magnesium alloys.展开更多
A social problem that low-income people are unable to purchase houses in urban areas has been focused in recent years and the housing security system has also become an important part of the social security system. In...A social problem that low-income people are unable to purchase houses in urban areas has been focused in recent years and the housing security system has also become an important part of the social security system. In recent years, public rental houses appeared as a new type of affordable houses. The national government took the construction of public rental houses to the national strategic level. Currently, there are many problems in the public rental house construction. For example, policy mechanisms are inadequate, the use of funding is not in an efficient way, the location of public rental houses is far away from the downtown, the plot ratio is too high, and facilities are incomplete. There are no appropriate indicators to comprehensively evaluate the public rental house construction. Based on the livable evaluation system in Chongqing, we selected four aspects of relevant indicators as the economic suitability, environmental suitability, social suitability, and life suitability to establish the public rental house construction suitability evaluation system.展开更多
This paper establishes a 3D numerical model for 15# hydropower house of the Three Gorges Project (TGP) and performs a nonlinear static and dynamic damage analysis. In this numerical model, a coupling model of finite a...This paper establishes a 3D numerical model for 15# hydropower house of the Three Gorges Project (TGP) and performs a nonlinear static and dynamic damage analysis. In this numerical model, a coupling model of finite and infinite elements for simulating infinite foundation of hydropower station is adopted. A plastic-damage model based on continuum damage mechanics, which includes the softening and damage behavior under tension is considered for the concrete material. The dynamic equilibrium equations of moti...展开更多
Pancreatic carcinomas with acinar differentiation are rare,accounting for 1%-2% of adult pancreatic tumors; they include pancreatic acinar cell carcinoma(PACC),pancreatoblastoma,and carcinomas of mixed differentiation...Pancreatic carcinomas with acinar differentiation are rare,accounting for 1%-2% of adult pancreatic tumors; they include pancreatic acinar cell carcinoma(PACC),pancreatoblastoma,and carcinomas of mixed differentiation. Patients with PACC have a prognosis better than pancreatic ductal adenocarcinomas but worse than pancreatic neuroendocrine tumors. Reports of overall survival range from 18 to 47 mo. A literature review on PACCs included comprehensive genomic profiling and whole exome sequencing on a series of more than 70 patients as well as other diagnostic studies including immunohistochemistry. Surgical resection of PACC is the preferred treatment for localized and resectable tumors. The efficacy of adjuvant treatment is unclear. Metastatic PACCs are generally not curable and treated with systemic chemotherapy. They are moderately responsive to chemotherapy with different regimens showing various degrees of response in case reports/series. Most of these regimens were developed to treat patients with pancreatic ductal adenocarcinomas or colorectal adenocarcinomas. Review of PACC's molecular profiling showed a number of gene alterations such as: SMAD4,BRAF,BRCA2,TP53,RB1,MEN1,JAK-1,BRCA-1,BRCA-2,and DNA mismatch repair abnormalities. PACCs had multiple somatic mutations with some targetable with available drugs. Therefore,molecular profiling of PACC should be an option for patients with refractory PACC.展开更多
Shape grammar has been used by many researchers to identify and/or define languages in architectural design. This paper presents a methodology developed using shape grammar to analyze the informal city, specifically F...Shape grammar has been used by many researchers to identify and/or define languages in architectural design. This paper presents a methodology developed using shape grammar to analyze the informal city, specifically Favela da Rocinha’s buildings, in Rio de Janeiro. The goal was finding the organizational foundations of self-built houses in an informal settlement. A methodology was developed to find composition patterns and to infer a set of rules which defines a shape grammar of Rocinha houses, allowing synthesis and analysis of low cost solutions for house designs. And above all, it highlights underlying design solutions produced empirically by inhabitants of favelas, emphasizing the diversity in favela’s architecture, and, that is also able to reflect the particular necessities of targeted population.展开更多
In this paper, we study the influence ofeco materials for roof insulation and fiber-reinforced mortar coatings on cooling loads of a home in dry tropical climate. The walls of the house are made of cinderblock or late...In this paper, we study the influence ofeco materials for roof insulation and fiber-reinforced mortar coatings on cooling loads of a home in dry tropical climate. The walls of the house are made of cinderblock or laterite and the insulating material of a roof panel is made with lime (24%), cement (6%), water (50%) of vegetable fibers hibiscus sabdariffa (16%), tree widespread in Burkina Faso and sugar cane bagasse (4%). This panel roof insulation and the fiber-reinforced mortar were characterized at the Laboratory of Physics and Chemistry of the environment by the hot plate method. The building is modeled in TRNSYS using climate data from the city of Ouagadougou. The results obtained show that in the warmer months of the year, that is to say in March and April, the relative differences between heat gains the configurations "breeze block-coating mortar and roof not insulated" and "laterite- fiber-reinforced mortar coating and insulated roof' vary between 15.6% and 16.8%. The configuration "laterite-fiber-reinforced mortar coating and insulated roof allows a reduction of annual heat gains of 15.5% compared to the configuration "breeze block-coating mortar and roof not insulated".展开更多
AIM To explore the induction effects and mechanism of Solanum lyratum Thumb(ST) on human hepatocellularcarcinoma SMMC-7721 cells through the mitochondrial pathway.METHODS The experiments were conducted on three groups...AIM To explore the induction effects and mechanism of Solanum lyratum Thumb(ST) on human hepatocellularcarcinoma SMMC-7721 cells through the mitochondrial pathway.METHODS The experiments were conducted on three groups: an experimental group (with ST ethanol extracts' concentration being 2.5, 5 and 10 mg/L), a negative control group (with only nutrient solution, 0 mg/L ST ethanol extracts), and a positive control group (2.5 mg/L DDP). The inhibition rate of cell proliferation was checked by using the methyl thiazolyl tetrazolium method, and cell apoptosis was tested by TUNEL method. Furthermore, RT-PCR was used to examine m RNA expression of Fas, Fas L, caspase-8, caspase-3, p53 and Bcl-2 genes.RESULTS Compared with the negative control group, the inhibition and apoptosis rates of the experimental group with different concentrations of ST extracts on human hepatocellular carcinoma SMMC-7721 cells significantly increased(P<0.05). Besides, the m RNA expression of Fas L and Bcl-2 significantly decreased(P<0.05) while the m RNA expression of Fas, caspase-8, caspase-3 and p53 increased significantly. When compared with the positive control group, the experimental groups with 5 mg/L ST ethanol extracts showed effects similar to the positive control group.CONCLUSION ST ethanol extracts induced the apoptosis of hepatocellular carcinoma SMMC-7721 cells through up-regulated Fas, caspase-8, caspse-3 and p53, and down-regulated Fas L and Bcl-2 in the mitochondrial pathway.展开更多
The House of the Scorpion (2002) is a National Book Award winner by American YA (young adult) novelist Nancy Farmer. It is a coming-of-age story about Matt, a young clone struggling for acceptance and survival in ...The House of the Scorpion (2002) is a National Book Award winner by American YA (young adult) novelist Nancy Farmer. It is a coming-of-age story about Matt, a young clone struggling for acceptance and survival in a dystopian world. Under the veil of science fiction, The House of the Scorpion not only allegorically exposes the evil of dehumanization, totalitarianism, and the abuse of technology, but also successfully builds up the brave image of Matt. Through depicting protagonist Matt's initiation journey in a dystopian world, Nancy Farmer expresses her belief that goodness triumphs and hope is indestructible.展开更多
The House of the Seven Gables (1981), Hawthorne's second romance, is his another masterpiece after The Scarlet Letter (1956). The achievement of its themes and narrative skills has been greatly complimented by re...The House of the Seven Gables (1981), Hawthorne's second romance, is his another masterpiece after The Scarlet Letter (1956). The achievement of its themes and narrative skills has been greatly complimented by readers, especially its descriptions and analyses of the characters' psychological activities and their personalities. The notorious Salem Witch Trials of Hawthorne's family has a great effect on Hawthorne's formation of his views about the good and evil, the sin and penalty, and the psychological descriptive style in his works. In The House of the Seven Gables, Hawthorne takes this family history as the original writing background. Through exploring modem people's spiritual essence, using history as narrative original, Hawthorne's theme illustrates the past's influence on the present. By exploring the changes of the characters' psychologies and values, especially those of Holgrave, the narrator proposes a conception of general love to eradicate the actual contradictions. Hawthorne actually provides an alternative way--a nonpolitical solution to the national problems, which demonstrates not only his artistic pursuit but also his culturally political view in his writing. This paper consists of five parts.展开更多
文摘This paper looks back to the development of plant tissue culture in China in the last century. Since 1934, tissue culture studies in China has kept up with the international development in the fields. Progress has been made by Chinese in nearly every branches of tissue culture, including in vitro organogenesis, shoot tip culture, anther culture, ovary culture, endosperm culture, protoplast culture as well as mass cell culture. On the basis of reviewing the articles written by Chinese on plant tissue culture, the internationally recognized contributions are specially mentioned. The applications of plant tissue culture to agriculture and industry in China are also introduced.
基金Supported by Program from Beijing Municipal Science & Technology Commission (Z080005032508017)~~
文摘[Objective] This study was to investigate the in vitro culture of unfertilized ovary of strawberry.[Methods] Employing single factor experiment,we investigated six key factors including genotype,extrinsic hormone,sucrose concentration,low temperature pretreatment,growth environment and development status,and illumination condition on induction of gynogenesis in vitro of unfertilized ovary,on the induction of gynogenesis in vitro of unfertilized ovary.[Results] The optimal conditions for in vitro culture of unfertilized ovary of strawberry were as follows:the primary flower buds cultured on bare land as explants,selection of appropriate genotype,2,4-D as external hormone,sucrose at the concentration of 6%,low temperature pretreatment for 48 hours and dark culture under alternated temperature.[Conclusion] The research provided reference for ploidy breeding in strawberry.
文摘Anomozamites haifanggouensis (Kimura et al.) Zheng et Zhang comb. nov. is a characteristic Bennettitalean plant in Middle Jurassic flora. Only one specimen was collected from Haifanggou Formation (Middle Jurassic) in Daohugou village, Shantou town, Ningcheng County, Nei Mongol Autonomous Region, China. The stem of plant is slender and repeatedly branches as a dichasial system with a fertile shoot in the fork, forming equal and widely divergent branches, on the branches born with the fronds of Anomozamites type. Its reproductive organs consists of some microsporophylls and a few of the bracteoid small leaves (or receptacular leaves). Some scattered bracteoid leaves and microsporophylls were formerly considered by Pan as dicots. Since then, they were proposed to be a non-committal genus Pankuangia and described them as P. haifanggouensis by Kimura et al. In this study, we discover that these bracteoid small leaves and microsporophylls should be of Anomozamites. This discovery provides strong evidence for the 'Pankuangia' determination of the taxonomical position.
基金Supported by the National Natural Science Foundation of China,No.81300294State Scholarship Fund of China,No.201509110033
文摘AIM To investigate the role of regulatory T cell(Treg) subsets in the balance between Treg and T helper 17(Th17) cells in various tissues from mice with dextran sulfate sodium-induced colitis.METHODS T r e g c e l l s, T r e g c e l l s u b s e t s, T h 1 7 c e l l s, a n d CD4+CD25+FoxP 3+IL-17+ cells from the lamina propria of colon(LPC) and other ulcerative colitis(UC) mouse tissues were evaluated by flow cytometry. Forkhead box protein 3(FoxP 3), interleukin 17A(IL-17A), and RORC m RNA levels were assessed by real-time PCR, while interleukin-10(IL-10) and IL-17 A levels were detected with a Cytometric Beads Array.RESULTS In peripheral blood monocytes(PBMC), mesenteric lymphnode(MLN), lamina propria of jejunum(LPJ) and LPC from UC mice, Treg cell numbers were increased(P < 0.05), and FoxP 3 and IL-10 mR NA levels were decreased. Th17 cell numbers were also increased in PBMC and LPC, as were IL-17 A levels in PBMC, LPJ, and serum. The number of FrI subset cells(CD4+CD45RA+FoxP 3low) was increased in the spleen, MLN, LPJ, and LPC. FrI I subset cells(CD4+CD45RA-Fox P3high) were decreased among PBMC, MLN, LPJ, and LPC, but the number of Fr III cells(CD4+CD45RA-FoxP 3low) and CD4+CD25+FoxP 3+IL-17A+ cells was increased. Fox P3 m RNA levels in CD4+CD45RA-Fox P3 low cells decreased in PBMC, MLN, LPJ, and LPC in UC mice, while IL-17 A and RORC mR NA increased. In UC mice the distribution of Treg, Th17 cells, CD4+CD45RA-FoxP 3high, and CD4+CD45RA-FoxP 3low cells was higher in LPC relative to other tissues.CONCLUSION Increased numbers of CD4+CD45RA-FoxP 3low cells may cause an imbalance between Treg and Th17 cells that is mainly localized to the LPC rather than secondary lymphoid tissues.
基金Supported by National Key Technologies R&D Program for the 11th Five-year Plan,No. 2008ZX10001-006
文摘AIM:To assess the peripheral T lymphocyte subsets in chronic hepatitis B virus(HBV) infection,and their dynamics in response to adefovir dipivoxil monotherapy.METHODS:Proportions and absolute counts of peripheral natural killer cells,B cells,CD8+,CD4+,CD8+ CD38+,CD8+CD28+ and CD4+CD28+ T cells were determined using three-color flow cytometry in chronic hepatitis B patients(n = 35),HBV carriers(n = 25) and healthy controls(n = 35).Adefovir dipivoxil was initiated in 17 chronic hepatitis B patients who were regularly followed for 72 wk,during which period the T cell subsets and serum viral load were measured at each follow-up point.RESULTS:The peripheral CD4+ T cell counts and CD8+ T cell counts decreased in chronic HBV infection.In chronic hepatitis B patients,proportions of CD8+CD38+ T cells were 62.0% ± 14.7%,much higher than those of HBV carriers and healthy con-trols.In the 13 hepatitis B patients who were treated and responded to adefovir dipivoxil,proportions of CD8+CD38+ T cells decreased from 53.9% ± 18.4% pre-therapy to 20.1% ± 11.3% by week 72(P < 0.001),concomitant with viral load decline(HBV DNA fell from 7.31 to 3 log copies/mL).CD8+ T cell counts also underwent an average increase of 218 cells/μL by the end of 72-wk treatment.In those who failed the therapy,the CD8+CD38+ T cell population had more fluctuations.CONCLUSION:CD8+ T cells abnormally activated in chronic HBV infection can be partially reversed by antiviral therapy.HBV-associated immune activation may be a crucial part of the pathogenesis and a promising target of treatment.
基金the National Natural Science foundation of China,No.81360330
文摘AIM to investigate the expression of proliferating cell nuclear antigen(p CNA)and E-cadherin in gastric carcinoma and to analyze their clinical significance.METHODS A total of 146 patients were selected for this study,including 38 patients with intestinal metaplasia,42with dysplasia,and 66 with primary gastric cancer.In addition,40 patients with normal gastric tissues were selected as controls.the expression of p CNA and E-cadherin was detected by immunohistochemistry.Differences in p CNA and the E-cadherin labeling indexes among normal gastric mucosa,intestinal metaplasia,dysplasia,and gastric carcinoma were compared.Subjects with normal gastric tissues were assigned to a normal group,while gastric cancer patients were assigned to a gastric cancer group.the difference in p CNA and E-cadherin expression between these two groups was compared.the relationship between expression of p CNA and E-cadherin and clinicopathological features was also explored in gastric cancer patients.furthermore,prognosis-related factors,as well as the expression of p CNA and E-cadherin,were analyzed in patients with gastric cancer to determine the 3-year survival of these patients.RESULTS the difference in p CNA and the E-cadherin labeling indexes among normal gastric mucosa,intestinal metaplasia,dysplasia,and gastric carcinoma was statistically significant(p<0.05).During the transition of normal gastric mucosa to gastric cancer,the p CNA labeling index gradually increased,while the E-cadherin labeling index gradually decreased(p<0.05).the p CNA labeling index was significantly higher and the E-cadherin labeling index was significantly lower in gastric cancer than in dysplasia(p<0.05).the expression of p CNA was significantly higher in the gastric cancer group than in the normal group,but E-cadherin was weaker(p<0.05).there was a negative correlation between the expression of p CNA and E-cadherin in gastric carcinoma(r=-0.741,p=0.000).p CNA expression differed significantly between gastric cancer patients with and without lymph node metastasis and between patients at different t stages.E-cadherin expression also differed significantly between gastric cancer patients with and without lymph node metastasis(p<0.05).High t stage and positive p CNA expression were risk factors for the prognosis of patients with gastric cancer(RR>1),while the positive expression of E-cadherin was a protective factor(RR<1).the sensitivity,specificity,and accuracy of p CNA positivity in predicting the 3-year survival of patients with gastric cancer were 93.33%,38.89%,and0.64,respectively;while these values for E-cadherin negativity were 80.0%,41.67%,and 0.59,respectively.When p CNA positivity and E-cadherin negativity were combined,the sensitivity,specificity,and accuracy were66.67%,66.67%,and 0.67,respectively.CONCLUSION Combined detection of p CNA and E-cadherin can improve the accuracy of assessing the prognosis of patients with gastric cancer.
基金Project(50504019)supported by the National Natural Science Foundation of ChinaProject(2008BB4040)supported by the Science Foundation of Chongqing,ChinaProject(2008AA4028)supported by Scientific and Technological Project of Chongqing Science and Technology Commission,China
文摘Electric product house of magnesium alloy sheet is usually obtained by warm stamping owing to its poor plasticity and formability at room temperature.The formability of AZ31B magnesium alloy sheet can be improved by repeated unidirectional bending(RUB)process through control of(0002)basal texture.Compared with as-received sheet,the Erichsen value(IE)of the sheet underwent RUB process increases to 5.90 from 3.53 at room temperature.It is also confirmed that cell phone houses could be stamped successfully in crank press with AZ31B magnesium alloy sheets underwent RUB process.It provides an alternative to the electronics industry in the application of magnesium alloys.
基金Funded by the Fundamental Research Funds for the Central Universities Project (No. CDJZR 10190003)
文摘A social problem that low-income people are unable to purchase houses in urban areas has been focused in recent years and the housing security system has also become an important part of the social security system. In recent years, public rental houses appeared as a new type of affordable houses. The national government took the construction of public rental houses to the national strategic level. Currently, there are many problems in the public rental house construction. For example, policy mechanisms are inadequate, the use of funding is not in an efficient way, the location of public rental houses is far away from the downtown, the plot ratio is too high, and facilities are incomplete. There are no appropriate indicators to comprehensively evaluate the public rental house construction. Based on the livable evaluation system in Chongqing, we selected four aspects of relevant indicators as the economic suitability, environmental suitability, social suitability, and life suitability to establish the public rental house construction suitability evaluation system.
基金Supported by National Natural Science Foundation of China (No.50679009)Key Laboratory for Scientific Research of Department of Education in Liaoning Province (No.2008S045)
文摘This paper establishes a 3D numerical model for 15# hydropower house of the Three Gorges Project (TGP) and performs a nonlinear static and dynamic damage analysis. In this numerical model, a coupling model of finite and infinite elements for simulating infinite foundation of hydropower station is adopted. A plastic-damage model based on continuum damage mechanics, which includes the softening and damage behavior under tension is considered for the concrete material. The dynamic equilibrium equations of moti...
文摘Pancreatic carcinomas with acinar differentiation are rare,accounting for 1%-2% of adult pancreatic tumors; they include pancreatic acinar cell carcinoma(PACC),pancreatoblastoma,and carcinomas of mixed differentiation. Patients with PACC have a prognosis better than pancreatic ductal adenocarcinomas but worse than pancreatic neuroendocrine tumors. Reports of overall survival range from 18 to 47 mo. A literature review on PACCs included comprehensive genomic profiling and whole exome sequencing on a series of more than 70 patients as well as other diagnostic studies including immunohistochemistry. Surgical resection of PACC is the preferred treatment for localized and resectable tumors. The efficacy of adjuvant treatment is unclear. Metastatic PACCs are generally not curable and treated with systemic chemotherapy. They are moderately responsive to chemotherapy with different regimens showing various degrees of response in case reports/series. Most of these regimens were developed to treat patients with pancreatic ductal adenocarcinomas or colorectal adenocarcinomas. Review of PACC's molecular profiling showed a number of gene alterations such as: SMAD4,BRAF,BRCA2,TP53,RB1,MEN1,JAK-1,BRCA-1,BRCA-2,and DNA mismatch repair abnormalities. PACCs had multiple somatic mutations with some targetable with available drugs. Therefore,molecular profiling of PACC should be an option for patients with refractory PACC.
文摘Shape grammar has been used by many researchers to identify and/or define languages in architectural design. This paper presents a methodology developed using shape grammar to analyze the informal city, specifically Favela da Rocinha’s buildings, in Rio de Janeiro. The goal was finding the organizational foundations of self-built houses in an informal settlement. A methodology was developed to find composition patterns and to infer a set of rules which defines a shape grammar of Rocinha houses, allowing synthesis and analysis of low cost solutions for house designs. And above all, it highlights underlying design solutions produced empirically by inhabitants of favelas, emphasizing the diversity in favela’s architecture, and, that is also able to reflect the particular necessities of targeted population.
文摘In this paper, we study the influence ofeco materials for roof insulation and fiber-reinforced mortar coatings on cooling loads of a home in dry tropical climate. The walls of the house are made of cinderblock or laterite and the insulating material of a roof panel is made with lime (24%), cement (6%), water (50%) of vegetable fibers hibiscus sabdariffa (16%), tree widespread in Burkina Faso and sugar cane bagasse (4%). This panel roof insulation and the fiber-reinforced mortar were characterized at the Laboratory of Physics and Chemistry of the environment by the hot plate method. The building is modeled in TRNSYS using climate data from the city of Ouagadougou. The results obtained show that in the warmer months of the year, that is to say in March and April, the relative differences between heat gains the configurations "breeze block-coating mortar and roof not insulated" and "laterite- fiber-reinforced mortar coating and insulated roof' vary between 15.6% and 16.8%. The configuration "laterite-fiber-reinforced mortar coating and insulated roof allows a reduction of annual heat gains of 15.5% compared to the configuration "breeze block-coating mortar and roof not insulated".
基金the Guangxi Key Disciplines(Pathogen Biology)[2013]16,Key Laboratory Cultivation Base of Universities in Guangxi(Guangxi Education Research[2014]6)the Science and Technology Research Projects of Universities in Guangxi in 2014,No.YB2014307+1 种基金the Guangxi Natural Science Fund Project,No.2013GXNSFAA019249,No.2014GXNSFBA118148the Scientific Research Project of The Department of Education of Guangxi Zhuang Autonomous Region,No.200810LX327
文摘AIM To explore the induction effects and mechanism of Solanum lyratum Thumb(ST) on human hepatocellularcarcinoma SMMC-7721 cells through the mitochondrial pathway.METHODS The experiments were conducted on three groups: an experimental group (with ST ethanol extracts' concentration being 2.5, 5 and 10 mg/L), a negative control group (with only nutrient solution, 0 mg/L ST ethanol extracts), and a positive control group (2.5 mg/L DDP). The inhibition rate of cell proliferation was checked by using the methyl thiazolyl tetrazolium method, and cell apoptosis was tested by TUNEL method. Furthermore, RT-PCR was used to examine m RNA expression of Fas, Fas L, caspase-8, caspase-3, p53 and Bcl-2 genes.RESULTS Compared with the negative control group, the inhibition and apoptosis rates of the experimental group with different concentrations of ST extracts on human hepatocellular carcinoma SMMC-7721 cells significantly increased(P<0.05). Besides, the m RNA expression of Fas L and Bcl-2 significantly decreased(P<0.05) while the m RNA expression of Fas, caspase-8, caspase-3 and p53 increased significantly. When compared with the positive control group, the experimental groups with 5 mg/L ST ethanol extracts showed effects similar to the positive control group.CONCLUSION ST ethanol extracts induced the apoptosis of hepatocellular carcinoma SMMC-7721 cells through up-regulated Fas, caspase-8, caspse-3 and p53, and down-regulated Fas L and Bcl-2 in the mitochondrial pathway.
文摘The House of the Scorpion (2002) is a National Book Award winner by American YA (young adult) novelist Nancy Farmer. It is a coming-of-age story about Matt, a young clone struggling for acceptance and survival in a dystopian world. Under the veil of science fiction, The House of the Scorpion not only allegorically exposes the evil of dehumanization, totalitarianism, and the abuse of technology, but also successfully builds up the brave image of Matt. Through depicting protagonist Matt's initiation journey in a dystopian world, Nancy Farmer expresses her belief that goodness triumphs and hope is indestructible.
文摘The House of the Seven Gables (1981), Hawthorne's second romance, is his another masterpiece after The Scarlet Letter (1956). The achievement of its themes and narrative skills has been greatly complimented by readers, especially its descriptions and analyses of the characters' psychological activities and their personalities. The notorious Salem Witch Trials of Hawthorne's family has a great effect on Hawthorne's formation of his views about the good and evil, the sin and penalty, and the psychological descriptive style in his works. In The House of the Seven Gables, Hawthorne takes this family history as the original writing background. Through exploring modem people's spiritual essence, using history as narrative original, Hawthorne's theme illustrates the past's influence on the present. By exploring the changes of the characters' psychologies and values, especially those of Holgrave, the narrator proposes a conception of general love to eradicate the actual contradictions. Hawthorne actually provides an alternative way--a nonpolitical solution to the national problems, which demonstrates not only his artistic pursuit but also his culturally political view in his writing. This paper consists of five parts.