Thirty nine isozymes in four tissues (mantle muscle, buccal bulb muscle, eye and liver) of Sepia esculenta were screened for enzymatic analysis using starch gel electrophoretic technique. Eighteen enzymes (G3PDH, LDH,...Thirty nine isozymes in four tissues (mantle muscle, buccal bulb muscle, eye and liver) of Sepia esculenta were screened for enzymatic analysis using starch gel electrophoretic technique. Eighteen enzymes (G3PDH, LDH, MDH, MEP, IDHP, PGDH, GRS, NP, AAT, CK, AK, EST, ALP, ACP, FBP, MPI, GPI and PGM) show strong activities and good convergence in zymogram. They are proved to be suitable genetic markers in Sepia esculenta. Among the tissues used, mantle muscle is the best for electrophoretic analysis of isozymes. Eye and liver are fairly good for some special enzymes, such as LDH, EST, MPI, etc. Twenty six loci are detected. The proportion of polymorphic loci is 0.115 in the Qingdao sample and 0.153 in the Rizhao sample ( P < 0.99 ). The mean values of the observed and expected heterozygosity per locus of Qingdao sample are 0.016 and 0.017 , while those of the Rizhao sample are 0.023 and 0.025 respectively.展开更多
This study used an eye-tracking methodology to investigate consumers＇ gazing behavior by focusing on how assessment design influences gazing behavior and decision time. Only one factor of test design was investigated...This study used an eye-tracking methodology to investigate consumers＇ gazing behavior by focusing on how assessment design influences gazing behavior and decision time. Only one factor of test design was investigated and each test contained three images. Eight participants were recruited. This study answered three questions： （1） Does the color of food influence gazing behavior？ （2） Does the color of food influence decision-making？ （3） Is gazing behavior related to decision-making behavior？ The results demonstrated that gazing behavior and decision time have a positive relationship with the image selected. Future research should consider the relationships between eye movements, cognitive goals, and tasks展开更多
文摘Thirty nine isozymes in four tissues (mantle muscle, buccal bulb muscle, eye and liver) of Sepia esculenta were screened for enzymatic analysis using starch gel electrophoretic technique. Eighteen enzymes (G3PDH, LDH, MDH, MEP, IDHP, PGDH, GRS, NP, AAT, CK, AK, EST, ALP, ACP, FBP, MPI, GPI and PGM) show strong activities and good convergence in zymogram. They are proved to be suitable genetic markers in Sepia esculenta. Among the tissues used, mantle muscle is the best for electrophoretic analysis of isozymes. Eye and liver are fairly good for some special enzymes, such as LDH, EST, MPI, etc. Twenty six loci are detected. The proportion of polymorphic loci is 0.115 in the Qingdao sample and 0.153 in the Rizhao sample ( P < 0.99 ). The mean values of the observed and expected heterozygosity per locus of Qingdao sample are 0.016 and 0.017 , while those of the Rizhao sample are 0.023 and 0.025 respectively.
文摘This study used an eye-tracking methodology to investigate consumers＇ gazing behavior by focusing on how assessment design influences gazing behavior and decision time. Only one factor of test design was investigated and each test contained three images. Eight participants were recruited. This study answered three questions： （1） Does the color of food influence gazing behavior？ （2） Does the color of food influence decision-making？ （3） Is gazing behavior related to decision-making behavior？ The results demonstrated that gazing behavior and decision time have a positive relationship with the image selected. Future research should consider the relationships between eye movements, cognitive goals, and tasks
