The genus Stenochironomus from China,including 10 species,is reviewed.Three species,S.koreanus Borkent,S.maculatus Borkent,and S.totifuscus Sublette are re-described and recorded from China for the first time.One spec...The genus Stenochironomus from China,including 10 species,is reviewed.Three species,S.koreanus Borkent,S.maculatus Borkent,and S.totifuscus Sublette are re-described and recorded from China for the first time.One species misidentification is corrected.A key to the males of Stenochironomus in China is given.展开更多
Endotribelos Grodhaus, 1987 is recorded for the first time in China. The adult male of E. redimiculum sp. nov. is described and illustrated based on specimens collected from Zhejiang Province. A key to the males of th...Endotribelos Grodhaus, 1987 is recorded for the first time in China. The adult male of E. redimiculum sp. nov. is described and illustrated based on specimens collected from Zhejiang Province. A key to the males of this genus worldwide is given.展开更多
AIM:To investigate trefoil factor(TFF) gene copy number,mRNA and protein expression as potential biomarkers in cholangiocarcinoma(CCA).METHODS:TFF mRNA levels,gene copy number and protein expression were determined re...AIM:To investigate trefoil factor(TFF) gene copy number,mRNA and protein expression as potential biomarkers in cholangiocarcinoma(CCA).METHODS:TFF mRNA levels,gene copy number and protein expression were determined respectively by quantitative reverse transcription polymerase chain reaction(PCR),quantitative PCR and immunohistochemistry in bile duct epithelium biopsies collected from individuals with CCA,precancerous bile duct dysplasia and from disease-free controls.The functional impact of recombinant human(rh) TFF2 peptide treatment on proliferation and epidermal growth factor receptor(EGFR) /mitogenactivated protein kinase(MAPK) signaling was assessed in the CCA cell line,KMBC,by viable cell counting and immunoblotting,respectively.RESULTS:TFF1,TFF2 and TFF3 mRNA expression was significantly increased in CCA tissue compared to disease-free controls,and was unrelated to gene copy number.TFF1 immunoreactivity was strongly increased in both dysplasia and CCA,whereas TFF2 immunoreactivity was increased only in CCA compared to diseasefree controls.By contrast,TFF3 immunoreactivity was moderately decreased in dysplasia and further decreased in CCA.Kaplan-Meier analysis found no association of TFF mRNA,protein and copy number with age,gender,histological subtype,and patient survival time.Treatment of KMBC cells with rhTFF2 stimulated proliferation,triggered phosphorylation of EGFR and downstream extracellular signal related kinase(ERK),whereas co-incubation with the EGFR tyrosine kinase inhibitor,PD153035,blocked rhTFF2-dependent proliferation and EGFR/ERK responses.CONCLUSION:TFF mRNA/protein expression is indicative of CCA tumor progression,but not predictive for histological sub-type or survival time.TFF2 is mitogenic in CCA via EGFR/MAPK activation.展开更多
The subgenus Nostocoladius of Cricotopus is recorded in Oriental China for the first time. The detail redeseription of Cricotopus （Nostocoladius） lygropis Edwards is presented. C. （N..） seiryuabeus Sasa, Suzuki ＆...The subgenus Nostocoladius of Cricotopus is recorded in Oriental China for the first time. The detail redeseription of Cricotopus （Nostocoladius） lygropis Edwards is presented. C. （N..） seiryuabeus Sasa, Suzuki ＆ Sakai is treated as a junior synonym of C. （N.） lygropis. A key to the known male imagines in the world of the subgenus Nostocoladius is given.展开更多
基金supported by the Zhejiang Provincial Natural Science Foundation of China (Y3100486)the National Natural Science Foundation of China (30570207, J0630963)Fauna of China (FY120100)
文摘The genus Stenochironomus from China,including 10 species,is reviewed.Three species,S.koreanus Borkent,S.maculatus Borkent,and S.totifuscus Sublette are re-described and recorded from China for the first time.One species misidentification is corrected.A key to the males of Stenochironomus in China is given.
基金supported by the National Natural Science Foundation of China (31301908, 31272284)the Zhejiang Provincial Natural Science Foundation of China (Y3100486)the Fauna of China (FY120100)
文摘Endotribelos Grodhaus, 1987 is recorded for the first time in China. The adult male of E. redimiculum sp. nov. is described and illustrated based on specimens collected from Zhejiang Province. A key to the males of this genus worldwide is given.
基金Supported by The Thailand Research Fund through the Royal Golden Jubilee PhD program(grant PHD/0121/2547 code 5LKK/47/B1 to Kosriwong K and Limpaiboon T)Khon Kaen University Research Affairs(grant 48-03-1-01-03)the Centre for Research and Development of Medical Diagnostic Laboratories,Faculty of Associated Medical Sciences(No.06-01), Thailand
文摘AIM:To investigate trefoil factor(TFF) gene copy number,mRNA and protein expression as potential biomarkers in cholangiocarcinoma(CCA).METHODS:TFF mRNA levels,gene copy number and protein expression were determined respectively by quantitative reverse transcription polymerase chain reaction(PCR),quantitative PCR and immunohistochemistry in bile duct epithelium biopsies collected from individuals with CCA,precancerous bile duct dysplasia and from disease-free controls.The functional impact of recombinant human(rh) TFF2 peptide treatment on proliferation and epidermal growth factor receptor(EGFR) /mitogenactivated protein kinase(MAPK) signaling was assessed in the CCA cell line,KMBC,by viable cell counting and immunoblotting,respectively.RESULTS:TFF1,TFF2 and TFF3 mRNA expression was significantly increased in CCA tissue compared to disease-free controls,and was unrelated to gene copy number.TFF1 immunoreactivity was strongly increased in both dysplasia and CCA,whereas TFF2 immunoreactivity was increased only in CCA compared to diseasefree controls.By contrast,TFF3 immunoreactivity was moderately decreased in dysplasia and further decreased in CCA.Kaplan-Meier analysis found no association of TFF mRNA,protein and copy number with age,gender,histological subtype,and patient survival time.Treatment of KMBC cells with rhTFF2 stimulated proliferation,triggered phosphorylation of EGFR and downstream extracellular signal related kinase(ERK),whereas co-incubation with the EGFR tyrosine kinase inhibitor,PD153035,blocked rhTFF2-dependent proliferation and EGFR/ERK responses.CONCLUSION:TFF mRNA/protein expression is indicative of CCA tumor progression,but not predictive for histological sub-type or survival time.TFF2 is mitogenic in CCA via EGFR/MAPK activation.
基金Financial support from the National Natural Science Foundation of China (31272284, 31201739, J1210005,31101624)the Zhejiang Provincial Natural Science Foundation of China (Y3100486)Fauna of China (FY120100)
文摘The subgenus Nostocoladius of Cricotopus is recorded in Oriental China for the first time. The detail redeseription of Cricotopus （Nostocoladius） lygropis Edwards is presented. C. （N..） seiryuabeus Sasa, Suzuki ＆ Sakai is treated as a junior synonym of C. （N.） lygropis. A key to the known male imagines in the world of the subgenus Nostocoladius is given.
