A Systematic Revision of "Barbodes" Fishes in China

The genus Barbodes Bleeker is a catchall group.The species assigned to this genus in China actually belong to at least four distinct genera.After comparing all the Barbodes fishes included in this research to the system used by Rainboth,reassign Barbodes fishes in China as following:①B.baoshanensis,B.benasi and B.heterostomus are assigned to genus Neolissochilus;②B.parvus are assigned as Discherodontus parvus;③B.pierrei in Yunnan is conspecific with B.vernayi and B.vernayi should be assigned to the genus Hypsibarbus as Hypsibarbus vernayi;④the remaining Barbodes fishes,B.fuxianhuensis,B.chonglingchungi,B.margarianus,B.huangchuchieni,B.cogginii (=B.daliensis),B.rhomboides,B.carinatus,B.opisthopterus and B.exiguus are assigned to genus Poropuntius.

A new blind loach species,Triplophysa huanjiangensis(Teleostei:Balitoridae),from Guangxi,China

A new blind loach of the genus Triplophysa was collected in 2007 from a cave in Leyi village,located near Chuanshan Town,Huangjiang County in the Guangxi Zhuang Autonomous Region of southern China.The new species,Triplophysa huangjiangensis sp.nov,can be distinguished from its congeners by eyes and scales absent;caudalfin forked;adipose keel present;lips with furrows;distal margin of dorsal-fin truncate,dorsal fin origin much closer to caudal-fin base than snout tip;8-9 branched dorsal-fin rays;6-7 branched pelvic-fin rays;6 branched anal-fin rays;10-11 branched pectoral-fin rays;13-14 branched caudal-fin rays;pectoral-fin length 52.3%-70.7% the distance between pectoral-fin origin to pelvic-fin origin;dorsal-fin origin posterior to vertical line of pelvic-fin origin;outer rostral barbel longer than other two pairs of barbels,with 47.0%-73.8% of lateral head length;anterior nostril with elongate barbel-like tip;and posterior chamber of gas-bladder developed,reaching pelvic-fin origin.

A New Record of Anguillid Fish in Lancangjiang River,China:Anguilla bicolor

One specimen was collected from Lancangjiang River in Jinghong,China on August 1st,2006.It is identified as Anguilla bicolor McClelland,1844,a new record of Anguillidae species occurring in Lancangjiang River in China.It could be distinguished from other Anguilla species by the character as：dorsal-fin origin located above vent vertically.It is an active nocturnal forager,feeding largely on a diet of crustaceans and mollusks.

A new record of Dasyatid fish in China: Dasyatis laosensis

One specimen was collected from the main course of Lancangjiang River （upper reach of Mekong） in Guanlei Port, Xishuangbanna, Yunnan, China in April, 2008. It is identified as Dasyatis laosensis, a new record of Dasyatidae species in China. It could be distinguished from other Dasyatis species by a combination of the following characteristics： one enlarged venomous spine on the tail, ventral surface of body with orange marginal coloration, tail length greater than body length.

A New Species of the Genus Garra from Nujiang River Basin, Yunnan, China (Teleostei: Cyprinidae)

A new species of the subfamily Labeoninae, Garra nujiangensis Chen and Yang, sp. nov. is recognized from a secondary branch of Nujiang River （upper Salween） in Zhenkang county, Yunnan province, China. It can be distinguished from other beardless Garra species by the following combination of characters： 12 - 14 circumpedunele scales; 48 - 50 lateral- line scales; a pair of rudimentary rostral barbels present in little individuals; 8 - 9 branched dorsal-fin rays; 5 - 6 scales between dorsal-fin origin and lateral line and 3 - 4 scales between pelvic-fin origin and lateral line; caudal peduncle relatively stouter; length between snout tip to posterior margin of eye longer than body depth at anal fin origin; head length longer than body depth at dorsal fin origin; prepelvic length 50.0% - 53.7% of standard length; pre-anus length 58.9% - 63.9% of standard length; body width 16.7% - 19.6% of standard length; body depth at dorsal fin 19.2% - 22.6% of standard length. The present study also verified that the presence of tiny barbells is a juvenile character in some beardless Garra species, such as G. nujiangensis and G. alticorpora.

ACUPOINT-LIKE STRUCTURE AND MERIDIAN-LIKE DISTRIBUTION ON THE BODY SURFACE OF PLATYFISH

Objective To probe into whether an acupoint-like and meridian-like structure was existed in platyfish. Methods Adult platyfish was put in 30 μM 4-Di-2-ASP water solution for 3 h, then, in pipe water for 20min, afterward, the fish was anesthetized in 10% aether water solution, and the fluorescent labeling was observed under fluorescent microscope with B-3A combination filter. Results The labels observed under the microscope were in round bright dot, a majority of dots were distributed separately and a part of them was in cluster （2- 5 dots/cluster） on various parts of the body in regular arrangement. The labels on the head were circularly distributed around the eye and two arches were formed posterior to the eye and in the inferior 3/4 quadrant. These two arches joined one arch in the anterior superior 1/4 quadrant. On the fish trunk, it was observed that the labels were distributed from the back to the abdomen along the longitudinal axis of the trunk, forming 6 lines, located on No. 2, 4, 6, 7, 8 and 9 scale rows successively on the dorsal part of fish. Each line was composed of 7 to 22 label clusters and 1 -5 labels were counted in each cluster. The labels were arranged as 3-4 lines on the tail. Conclusion 1） Acupoint-like and meridian-like structure was existed in platyfish. 2）The skin sensory organs of animal were not distributed evenly all over the body. Instead, a number of sensory organs were put together in cluster and a number of them were in linear distribution regularly along the long axis of the trunk, which was similar to the distribution of traditional meridians and acupoints.

RAPD-SCAR Markers for Genetically Improved NEW GIFT Nile Tilapia (Oreochromis niloticus niloticus L.) and Their Application in Strain Identification

The NEW GIFT Nile tilapia （Oreochromis niloticus niloticus L.） is a nationally certificated new strain selected over 14 years and 9 generations from the base strain of GIFT Nile tilapia, introduced in 1994. This new variety has been extended in most of areas of China. The management of genetically improved strains, including the genetic markers for identification is needed urgently. RAPD analysis was conducted and their conversion to SCAR markers was developed. From NEW GIFT Nile tilapia, two strain-specific RAPD bands, S304624 bp and S36568 bp were identified. The strain-specific RAPD bands were gel-purified, cloned, and sequenced. Locus-specific primers were then designed to amplify the strain-specific bands. PCR amplification was conducted to test the variations in allele frequencies of two converted SCAR markers among the NEW GIFT Nile tilapia and its base strains, as well as 7 additional farmed strains worldwide. The frequency of SCAR marker Ⅰ （553 bp） was 85.7% in NEW GIFT Nile tilapia, but 16.7% in the base strain. The frequency of SCAR marker Ⅱ （558 bp） was 91.4% in NEW GIFT Nile tilapia, but 0% 70% in the 7 other strains. In order to confirm the utility of these two markers, an examination was conducted for a wild population from Egypt, resulted the frequency of SCAR Ⅰ and Ⅱ was 10% and 70%, respectively, much lower than that of New GIFT strain. The increase in allele frequency of these two SCAR markers suggests that these markers might be genetically linked to the quantitative trait loci （QTL） underlining the performance traits by long term selection, and indicate the bright potential of SCAR marker technology for tracking generations during selection progress and for distinguishing among genetically improved strain and other strains.

新鱼老鱼的不同胃口

所谓的“老鱼”和“新鱼”并非指鱼的大小或年龄，而是指鱼定居某水域的时间长短。

Discovery of Tanichthys albonubes Lin 1932 (Cyprinidae) on Hainan Island,and Notes on Its Ecology

A wild population of the globally restricted and highly threatened freshwater fish Tanichthys albonubes Lin was recently discovered on Hainan Island, China. Prior to the present study, native populations were only known from isolated pockets in Guangdong Province of China and Quang Ninh Province of Vietnam; the Hainan record thus represents the first insular and southernmost distribution of this little-known species. Thus far it is known only from a single site in Hainan, despite repeated surveys of similar habitats throughout the island. The Hainan population occurs in a clean, sluggish coastal stream with abundant hydrophytes, and is sympatric with 20 fish species. Due to the unique geographic distribution of the Hainan population, and the conservation significance of the species, studies of its phylogenetic relationships with conspecifics populations elsewhere are underway. The exact locality of the newly discovered Hainan population cannot be revealed, but relevant government authorities have been notified and we are working to protect the site.

Diet and feeding ecology of invasive icefish Neosalanx taihuensis in Erhai Lake,a Chinese plateau mesoeutrophicated lake

A comprehensive study of the invasive icefish Neosalanx taihuensis feeding ecology in Erhai Lake was conducted from November 2009 to October 2010. Prey items in the guts of the icefish sampled varied significantly according to season. This finding suggests a relationship between fluctuations in available prey in the environment and selective feeding by icefish. N. taihuensis preferred large-sized zooplankton, such as Daphnia and calanoids. Additionally, the gut fullness values differed significantly （P〈0.001） among sampling times. To compare the values at different times, samples were taken over a 24 h period every 2 months for the entire year. The feeding activities of the fish were concentrated either in the morning （8：00） and/or at dusk （20：00）, except in September 2010. This finding can be explained primarily by the variation in optimum light intensity. Daily N. taihuensis zooplankton consumption varied significantly, both diurnally and among seasons. The daily consumption values ranged from 0.089 to 0.237 g （wet weight） per 100 g wet fish weight in temperatures between 11.50℃ and 24.68℃. This is the first report of diel feeding periodicity and daily food consumption of icefish in their invaded ecosystems.

The Relation of Standard Metabolic Rate to Water Temperature and Body Weight of Schlegels Black Rockfish(Sebastodes Fuscescens)

Standard metabolic rates of Schlegels black rockfish with different body weights are determined in laboratory by using the flow-through respirometer at 11.2 ℃, 14.7℃ , 18.0 ℃ and 23.6 ℃ . The results indicate that the standard metabolic rates increase with the increase of body weight at different temperatures. Relationship between them could be described as Rs = a ln W b. The mean of standard metabolic rate is significantly different among groups, but the b values are not. The standard metabolic rates of amended standard body weights decrease with the increase of temperature, and the mean of standard metabolic rate is also significantly different among groups when the standard body weights are 48.6 g, 147.9 g, and 243.1 g. Relationship between them could be described as Rsw = m e-b/T. The relations of standard metabolic rate ( Rs ) or relative metabolic rate ( Rs ) to body weight and temperature yield the following equations: Rs = 1.160 W 0.752 e -9.494/ T and Rs’= 1.160 W 0.254 e -9.494/ T.

A redescription of grey pomfret Pampus cinereus (Bloch, 1795) with the designation of a neotype (Teleostei: Stromateidae)

Pampus cinereus （Bloch, 1795） （Stromateidae）, a species believed to be widely distributed throughout the Indo-Western Pacific region, was redescribed and a neotype was designated. The designation of a neotype was necessary because of ambiguous data in Bloch＇s original description and the loss of the original type specimen. Morphological data indicated that 10 recently-collected specimens from the coasts of southem China agreed well with Bloch＇s original description and figure ofP. cinereus. A neotype for this species was selected from among the 10 specimens, and a detailed description is presented in this paper.

Determination of Residual Enrofloxacin in Fish Tissues by Terbium Ion-sensitized Fluorophotometry

In order to establish a new method for the determination of enrofloxacin, on the basis of the fluorescence characteristic of terbium-enrofloxacin complex, it was found that in a HAc-NaAc buffer solution with a pH value at 6.0, the terbiumenrofloxacin complex had a characteristic fluorescence peak of Tb3＋ at 545 nm （λex= 328 nm）, which could be used for the determination of enrofloxacin. A simple, rapid and sensitive fluorescence method for the determination of enrofloxacin was thus established, and an optimal reaction condition was selected. Under the optimal reaction condition, there was a good linear relation （r2=0.992 3） between concentration of enrofloxacin in the range of 1.0×10^-1.0×10^-6 g/ml and its fluorescence intensity at 545 nm, with a detection limit of 1.3×10^-9 g/ml; the recovery for enrofloxacin in tablets was 97.7% with a variation coefficient of 1.4%; and for enrofloxacin in fish tissues, the recovery was 79.0%-94.5% with a variation coefficient of 2.0%-7.8%.

A new species,Gerres septemfasciatus(Perciformes:Gerreidae) from the Chinese coastal waters of the South China Sea

We describe a new species of gerreid fish,Gerres septemfasciatus,based on four specimens collected from the northern South China Sea.G.septemfasciatus most closely resembles G.limbatus in general appearance.However,G.septemfasciatus is distinguished from the latter and other congeners by having 3 to 3.5 scales between the base of the fifth dorsal spine and lateral line.This species has a distinctive color pattern,including 7-8 regular,vertical,blue-grayish bands on its side.The distribution of this species is currently known to include the Chinese coastal waters of the South China Sea,but may be also include the coastal waters of southeastern Asia.

Revision of Cyprinus maomingensis Liu 1957 and the first discovery of Procypris-hke cyprinid(Teleostei, Pisces) from the late Eocene of South China

Fossil cyprinids from the upper part of the upper Eocene Youganwo Formation of Maoming, Guangdong, China were first studied in 1957 by Liu, who referred the only specimen to the genus Cyprinus as a new species, C. maomingensis. And this was suggested as one of the earliest records for fossil cyprinids. Unfortunately, this specimen is poorly preserved and reveals no more morphological information than its serrated last unbranched dorsal and anal fin rays. Recently, some new specimens were unearthed from the same locality, where C. maomingensis was discovered. In addition to the serrated dorsal and anal fin rays, these new materials also show that the pattern and shape of their pharyngeal teeth obviously differ from that of Cyprinus but resemble that of Procypris. However, its number of the branched dorsal fin rays and number of vertebrae are much less than that in Procypris. Morphologically, these specimens are closer to Procypris than to Cyprinus. This is the first report of fossil Procypris-like fish, and it implies that Procypris-like fish is an early member of the Tribe Cyprinini sensu stricto(sensu Yang et al., 2010) and the origin of this group can be traced back at least to the late Eocene.

A new early cyprinin from Oligocene of South China

Here we describe tHuashancyprinus robustispinus, gen. et sp. nov, a fossil cyprinin fish from the Oligocene of Ningming Ba- sin, Guangxi, South China. It differs from all other cyprinin fishes in the following apomorphic characters： extremely robust last unbranched dorsal and anal fin rays with very fine serrations along posterior edge, the crowns of pharyngeal teeth A2 and A3 with a number of deep grooves on the lateral wall, rather large A3, and relatively deep infraorbital 2. Among the members of the Tribe Cyprinini sensu stricto, it mostly resembles the extant genus Cyprinus, particularly to species Cyprinus micristius and C. fuxianensis, which are restricted to Yunnan, southwestern China. The discovery of the cyprinin Huashancyprinus from southern China, along with the previously known late Eocene tCyprinus maomingensis, indicates an early branching of the Cyprininae （Cyprinidae） in this area.