毛泽东文章“新鲜”观的文风性质和现实意义

必须把毛泽东的文章"新鲜"观作为毛泽东文风理论的基本内容之一看待。毛泽东的文章"新鲜"观包括观点要新鲜独到、结构要不拘一格、要用自己的话写文章这么三个主张。目前,在文风领域里,毛泽东的文章"新鲜"观仍有可能起到一定的指导和批判作用,需要继续研究和宣传。

Regional Division of Production and Development Strategy of Citrus in Hunan Province

[Objective] The aim was to research regional division of citrus production and development strategies in Hunan Province. [Method] According to Rural Statisti- cal Yearbook in Hunan Province during 2002-2011, Citrus in Hunan province were divided into three production regions, namely, superior region, sub-superior and non- superior region. On the base of the divisions, the ecological regionalization and brand strategy, Optimization of regional distribution and developing strategy were proposed, with consideration of avoiding frozen zones, in this paper. [Result] Fresh and processing bases of mandarin orange （C.unshiu Marc）, and specialty industries of seedless ponkan（C.reticulata Blanco）, Bingtang orange （C.sinensis Osbeck Bing- tangcheng）, Dayongjuhuaxinyou （Cgrandis （L.） Osbeck Dayongjuhuaxinyou） and An- jiangxiangyou （C.grandis （L.） Osbeck Anjiangxiangyou） should be constructed, where fresh fruit is dominant, supplemented by canned fruit and juice. Industry belt of sat- suma orange, fresh or processing food, is mainly built, for proportion of early and earlier ripe satsuma orange is over 50% of total yield in Xiangzhong citrus zone; in- dustry belt of excellent fresh navel orange and processing sweet orange should be highlighted in Xiangnan. [Conclusion] The research provides references for decision- making for governments, especially on optimization of citrus production regions and development of citrus industry.

Discussion on Development of Pumpkin Sausage

[Objective] This study aimed to develop pumpkin sausage. [Method] The single-factor design and orthogonal design were adopted. In the traditional sausage,fresh pumpkin was added. Based on the results of sensory test and physical and chemical indexes determination, the optimum recipe for pumpkin sausage was determined. [Result] The optimum recipe for pumpkin sausage was as follows： 100% of pork（fat to lean ratio of 2∶8）, 45% of pumpkin particles, 6% of soy protein, 7% of sugar, 3.5% of salt, 1% of spice and 0.5% of monascus red pigment. [Conclusion]The successful preparation of pumpkin sausage will provide consumers with nutrition, health and snack meat product with comprehensive nutrition and unique flavor.

Research Progress in Formation and Detection Method of Dextran in Sugarcane Production

Dextran in sugarcane production process is formed by Leuconostoc rnesenteroides. The content levels of dextran is related to sugarcane varieties, field condition （planting pattern, temperature, humidity, sunlight, soil, foreign material）, de- gree of injury （refractory cane, harvesting methods）, and can be rapidly and accu- rately measured by Dextran Immunonephelometric Test Kit. The presence of dextran indicates that sucrose has been lost, so sugarcane dextran is a direct and reliable indicator to measure sugarcane freshness and quality.

Changes in the Contents of ATP and Its Related Breakdown Compounds in Various Tissues of Oyster During Frozen Storage

The changes in the contents of adenosine 5’-triphosphate (ATP) and its related breakdown compounds were investigated in the adductor muscle, mantle, gill and body trunk of oyster (Crassostrea gigas) during frozen storage at -20℃ and -30℃ and compared with that of the fresh oyster. The investigation was performed using an HPLC system. Different extents of ATP decomposi- tion were found in various tissues frozen at the two temperatures. The K, K’ and A.E.C values were calculated as the chemical fresh- ness indices. Considering the results of sensory evaluation, the A.E.C. value in body trunk at -20℃ and -30℃ could be used as the best freshness index for frozen oyster.

Fresh Frozen Plasma for the Treatment of Hereditary Angioedema Acute Attacks

Objective To determine the safety and efficacy of fresh frozen plasma （FFP） iniusion for the treat- ment of hereditary angioedema （FIAE）. Methods The medical records of patients with HAE admitted to Peking Union Medical College Fiospital who had received FFP infusion during 2004 and 2010 were reviewed and PubMed database iFom 1966 to the present were searched using the following key words： hereditary angioedema and fresh frozen plasma. The patient＇s age, sex, body location of HAE attacks, the dose of FFP infusion, time of beginning to improvenaent, time to complete remission, complication, C 1 inhibitor activity, and outcome were analyzed. Results A total of 13 enrolled patients （7 male and 6 female） received 16 times of FFP infusion, in- cluding 2 patients undergoing FFP infusion in Peking Union Medical College Hospital and 11 patients re- ported in the literature. The mean dosage of FFP infusion was 586±337 mL. Two cases suffered from wors- ening abdominal pain and one case experienced skin rash. Only I patient had no improvement in symptom owing to transfusion related reaction. There was a definite improvement in symptom 49± 19 minutes after beginning FFP infusion. The remission time decreased from 61.7±27.0 hours to 3.3 （2.0, 12.0） hours after FFP infusion. FFP infusion was effective for both type I and type Ⅱ HAE. Conclusion FFP seems to be safe and effective for acute attacks of HAE.

A case-control study on the relationship between salt intake and salty taste and risk of gastric cancer

AIM: To investigate the relationship between salt intake and salty taste and risk of gastric cancer. METHODS: A 1:2 matched hospital based case-control study including 300 patients with gastric cancer and 600 cancer-free subjects as controls. Subjects were interviewed with a structured questionnaire containing 80 items, which elicited information on dietary, lifestyle habits, smoking and drinking histories. Subjects were tested for salt taste sensitivity threshold (STST) usingconcentrated saline solutions (0.22-58.4 g/L). Conditional logistic regression was used to calculate odds ratios (ORs) and 95% confidence intervals (95% CI). RESULTS: Alcohol and tobacco consumption increased the risk of gastric cancer [OR (95% CI) was 2.27 (1.27-4.04) for alcohol and 2.41 (1.51-3.87) for tobacco]. A protective effect was observed in frequent consumption of fresh vegetable and fruit [OR (95% CI) was 0.92 (0.58-0.98) for fresh vegetable and 0.87 (0.67-0.93) for fruit]. Strong association was found between STST ≥ 5 and gastric cancer [OR = 5.71 (3.18-6.72)]. Increased STST score was significantly associated with salted food intake and salty taste preference (P < 0.05). CONCLUSION: A high STST score is strongly associated with gastric cancer risk. STST can be used to evaluate an inherited characteristic of salt preference, and it is a simple index to verify the salt intake in clinic.

Capillary gas chromatographic determination of dimethachlon residues in fresh tobacco leaves and cut-tobacco

Simple procedures for extraction and chromatographic determination of dimethachlon residues in fresh tobacco leaves and cut-tobacco are described.The determination was carried out by capillary gas chromatography(GC) with electron capture detection(ECD) and confirmed by GC-MS.The mean recoveries and relative standard deviation(RSD) were 93.2%～112.9% and 3.5%～6.7%,respectively at levels ranging from 0.01 to 0.1 mg/kg.The limit of determination was 0.001 mg/kg.Tobacco samples in routine check were successfully analyzed using the proposed method.

Fresh Frozen Plasma for the Treatment of a Chinese Patient with Hereditary Angioedema

HEREDITARY angioedema （HAE） is an autosomal dominant inherited condition which was initially described by Osier in 1888.1 Patients with HAE can develop rapid subcutaneous or submucosal edema involving the hands, feet, limbs, face, intestinal tract, even larynx and trachea. The mortality of an acute attack of HAE without treatment was reported as high as 30%.2 HAE is caused by the deficiency of Cl esterase inhibitor （CIINH） which results in episodes of edema in parts of the human body,

Spontaneous combustion tendency of fresh and pre-oxidized sulfide ores

Three representative sulfide ore samples were collected from typical metal mines,and their corresponding pre-oxidized products were obtained under nature environment.The thermal behaviors of each sample at heating rates of 5,10,15 and 20 °C/min in air flow from ambient temperature to 800 °C were studied by simultaneous thermal analysis and the TG/DSC curves before and after the pre-oxidation were compared.By the peak temperature of DTG curves,the whole reaction process for each sample was divided into different stages,and the apparent activation energies were calculated by the Ozawa-Flynn-Wall method.The results show that the reaction process of each sample after pre-oxidation is more complex,with quicker reaction rates,fewer heat production quantities,and higher or lower ignition-points.The apparent activation energies decrease from 364.017-474.228 kJ/mol to 244.523- 333.161 kJ/mol.Therefore,sulfide ores are more susceptible to spontaneous combustion after the pre-oxidation.

Influence of social upbringing on the activity pattern of captive lion Panthera leo cubs:Benefits of behavior enrichment

The influence of social upbringing on the activity pattern of lion Panthera leo cubs was investigated at three sites. In this study, stimulus objects such as sticks, grass, fresh dung （elephant Loxondota africana, zebra Equus quagga, impala Aepyceros melampus, duiker Sylvicapra grimmia, kudu Tragelaphus strepsiceros, giraffe Giraffa camelopardalis and wildebeest Connochaetes taurinus） and cardboard boxes, were utilized in an enrichment program aimed at encouraging active behaviors of captive lion cubs at Antelope Park and Masuwe. Lion cubs at Chipangali were not behaviorally enriched. Activity patterns were recorded for 10 days at each site. We recorded moving, resting, playing, grooming, visual exploration and display of hunting instincts. We found that behavioral enrichment enhanced the active behaviors of captive lion cubs. Orphan-raised cubs spent more time moving, playing and displaying hunting instincts than mother-raised cubs, but the time spent grooming was similar across areas and suggests that grooming is not influenced by enrichment. Mother-raised cubs spent more time engaged in visual exploration than orphan-raised cubs and this could be a behavior acquired from mothers or a result of confidence to explore because of their presence. Activity patterns were different among time treatments across our three study sites. Based on these findings, we suggest that lion cubs raised in captivity could benefit from behavioral enrichment to encourage active behaviors essential for eventual reintroduction into the wild

Long-Term Frozen Storage Impact on the Antioxidant Capacity and Chemical Composition of Sardinian Myrtle （Myrtus communis L,） Berries

The effects of frozen storage at -18 ℃ up to 6 months, on the antioxidant properties, chemical composition and other physicochemical properties of myrtle berries （Myrtus comrnunis L.） has been evaluated. Berries showed a good resistance to storage decay, and weight loss remained after six months in the 15% range. Results showed that frozen storage up to 6 months affect individual antocyanins content in different ways. Nevertheless the total anthocyanins content was at the end of storage higher than in fresh fruits. The antioxidant capacity calculated with 2,2-diphenyl-l-picrylhidrazyl （DPPH） and 2,2＇-Azino-bis （3-ethylbenzothiazoline-6-sulfonic acid） （ABTS） increased during storage and the calculated r2 showed a positive correlation with the total anthocyanins content. Gluconic and fumaric acid did not changed, quinic acid decreased while malic and shikimic acid increased. The total organic acid content during storage was higher than in fresh fruits causing a slight decrease in pH. Fructose and glucose increased with the same rate and the ratio fructose/glucose remained unchanged （1.06 ± 0.01）, CIE L^＊a^＊b^＊ coordinates showed a shift of the colour to tawny brown after six months storage.

Probing into Surface Sites of Fresh Mo_2N/Al_2O_3,Mo_2C/Al_2O_3 and MoP/Al_2O_3 Catalysts by CO Adsorption and In Situ FT-IR Spectroscopy

The surface nature of fresh Mo2N/Al2O3, Mo2C/Al2O3 and/MoP/Al2O3 catalysts, which were synthesized directly in the IR cell to avoid passivation, were characterized by in situ IR spectroscopy with CO as a probe molecule. CO adsorbed on fresh catalysts showed characteristic IR bands at 2045 cm-1 for Mo2N/Al2O3 catalyst, 2054 cm-1 for MozC/Al2O3 catalyst and 2037 cm-1 for MoP/Al2O3 catalyst, respectively. A strong band at 2200 cm-1 for Mo2N/Al2O3 catalyst, which could be ascribed to NCO species formed when CO reacted upon surface active nitrogen atoms, and a weak band at 2196 cm-1 for Mo2C/Al2O3 catalyst, which could be attributed to CCO species, were also detected. CO adsorbed on fresh Mo2N/Al2O3 catalyst, Mo2C/Al2O3 catalyst and MoP/Al2O3 catalyst, showed strong molecular adsorption, just like noble metals. Our experimental results are bolstered by direct IR evidence demonstrating the similarity in surface electronic property between the fresh Mo2N/Al2O3, Mo2C/Al2O3 and MoP/Al2O3 catalysts and noble metals.

Water Soluble Propolis and Royal Jelly Enhance the Antimicrobial Activity of Honeys and Promote the Growth of Human Macrophage Cell Line

Due to the overuse and misuse of antibiotic, an increase in antibiotic resistance of pathogenic bacteria is evolving. Attention should be focused on natural alternatives to antibiotics, like propolis, royal jelly （R J） and honeys. They all have strong antibacterial properties due to the active substances they contain. This study investigated the effect of combination of water soluble propolis （WSP） Greitl20 or fresh royal jelly （F-RJ） （MiZigoj） and Forest honeys as antibacterial against Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Acinetobacter baumanii, Staphylococcus aureus, methicillin resistant Staphylococcus aureus （MRSA）, Streptococcus pyogenes, Streptococcus agalactiae and Candida albicans. These substances are also cell growth promoters for human macrophage （TLT） cell line. WSP Greitl20, F-RJ （M） and different Forest honeys were prepared in saline as 10% solutions. The antimicrobial activity was expressed as the minimal inhibitory concentration （MIC） in mg/mL. The growth promotion activity was measured at optical density （OD） 595 nm. The combination ofWSP Greitl20 with different Forest honeys is better than F-RJ （M） in same combination with different Forest honeys. The best antibacterial/antifungal activity was found with the combination of 10% WSP Greit 120 in the Forest honey （1：10） from Italy or Spain. When measuring the growth promoting activity of TLT cell line, the best activity was detected at the combination of 10% WSP Greitl20 in the Forest honey from Italy （GI3 = 0.796 ± 0.014 and GI5 = 1.133± 0.022）. Antimicrobial and growth promoting activities are correlated and WSP-dependent.

Biochar for Soil Management： Effect on Soil Available N and Soil Water Storage

Soil management technologies for climate change adaptation and mitigation are needed to increase and sustain food production in smallholder agriculture while sequestering inert carbon in the soil. In a field studies at Crops Research Institute, Kwadaso-Kumasi Ghana, a control treatment, five inorganic fertilizer combinations （P30K60, N60P30K60, N120P30K60, NlsoP30K60 and N24oP3oK6o） and four biochar rates ＋ inorganic fertilizer （2 t/ha Biochar ＋ N60P30K60, 4 t/ha Biochar ＋ N60P30K60, 6 t/ha Biochar ＋ N6oP3oK6o and 8 t/ha Biochar ＋N6oP3oK6o） were assessed for their effect on soil moisture storage, soil available nitrogen and crop yield. The test crop was okra. Biochar amendments increased soil moisture storage by 14% relative to sole inorganic fertilizer applications. Biochar ＋ inorganic fertilizer relative to sole inorganic fertilizer increased soil available nitrate concentration by 85% at 0-15 cm soil depth but decreased soil ammonium-N by 71%. Compared to control, inorganic fertilizer （P3oK6o） resulted in more than 100% increase in okra fresh fruit yield. Addition of 60 kg N/ha to P3oK6o caused 23% decline in okra fresh fruit yield but showed 60% more okra fresh fruit yield than the control. Inorganic N rates of 120,180 kg N/ha and 240 kg N/ha combined with P3oK6o however caused a decline of 74% in okra fresh fruit yield. Biochar ＋ inorganic fertilizer increased okra fresh fruit yield by 100% compared to sole inorganic fertilizer. Biochar, an inert carbon, combined with inorganic fertilizer has tremendous potential to address food insecurity through soil moisture storage and soil N availability.

Effect of Sources and Concentrations of Soybean Phosphatidylcholine on Diluted Goat Semen Equilibrated at 4 ℃

The objective of the present study was to determine the proper sources and concentrations of soybean lecithin （phosphatidylcholine, PC） to be used as substitute for hen egg yolk in extender for preserving goat semen. Two sources of soybean lecithin （20% and 95% soybean phosphatidylcholine; PC20 and PC95） and three concentrations （1%, 2% and 3% v/v） of PC20 and PC95 supplemented in Tris-citric acid-fructose （TCF） extender were tested. The TCF extender supplemented with 20% hen egg yolk was used as a control. Fresh semen samples were collected from 3 goats by artificial vagina. Seminal plasma was removed by centrifugation and sperm pellets were pooled together and divided into 7 groups according to types of extender. The diluted semen samples were kept at 4 ℃ （equilibration）. The semen qualities including progressive motility, sperm viability, sperm plasma membrane integrity and tail abnormalities were evaluated before dilution and after 4 hrs equilibration. It was found that the progressive motility of equilibrated semen in egg yolk and PC20 extenders were higher than those in PC95 extender （P 〈 0.05）. Sperm viability was lower in 1% and 2% PC95 extender compared to other extenders （P 〈 0.05）. PC20 extender maintained the sperm membrane integrity and normal tail morphology at low temperature better than egg yolk and PC95 （P 〈 0.05）. It can be concluded that 20% soybean phosphatidylcholine supplemented in TCF extender at 1%-3% （v/v） is as effective as hen egg yolk to preserve goat semen during equilibration at 4 ℃ for 4 hrs .

Seasonal changes of bioelements in litter and their potential return to green leaves in five species of tropical dry deciduous forest, western India

The Litter nutrient concentrations of N, P, Ca, Mg, K and Na in mature leaves, twigs and reproductive parts and their relationship between senescent and young leaves were investigated in five forest species： Acacia nilotica, Acacia leucophloea. Tectona grandis, Miliusa tomentosa and Butea monosperma in Indian tropical dry deciduous forest in Western India.. Total dry matter of plant species was recorded and analyzed for N, P, Ca, Mg, K and Na. A. nilotica had the highest concentrations of N in leaf, while ,4. leucophloea had the highest concentrations of Ca and Mg in leaf. The highest concentrations of P in leaf were found in A. nilotica, A. leucophloea and B. monosperma where as lowest in T. grandis and M. tomentosa. No significant differences in K and Na were registered among the species. A marked seasonal variability was ob- served in the concentrations of N, P and K, except for Ca and Mg. Potas- sium is the single element that undergoes leaching and mobilization in all species. Resorped N and P can be used for the production of fresh leaf in the following annual cycle. Nutrient resorption and retranslocation from senescent leaves and litter supports the production of new foliage and increase the fertility of soil.

Change of Phosphate Fraction during Composting of Fresh Waste Leaves of Tobacco

Environmental issue has concerned the public with the build-up of soil phosphorus(P) following application of organic manure.This demands a fully understanding of the change of phosphorus during composting of fresh waste leaves of tobacco and finding out the status of P fertilizer in leaf-made manure.This study had shown that water-soluble and HCl-soluble phosphon were the dominant fractions of P in the compost of fresh waste leaves,in the ranges of 19%-41% and 17%-43%,respectively.However,the former declined progressively upon composting time,but the latter increased,indicating transformation of the more vulnerable water soluble P to the more recalcitrant HCl-extractable P.

Chemical and Biological Characterization from Condafia microphylla Fruits, a Native Species of Patagonia Argentina

Condalia microphylla Cav. （Rhamnaceae）, popularly known as ＂piquillin＂, is widely distributed in Patagonia. The drupes are consumed as fresh fruits by Argentine communities. The aim of this work was to quantify the nutritional value of C. microphylla fruit and the phenolic compounds present and to determine the functional antioxidant properties in vitro and in vivo. The nutritional value was determined according to the Association of Official Analytical Chemists （AOAC） methodology, and phenolic compounds were quantified by diode-array detection （HPLC-DAD）. Antioxidant activity in vitro and in vivo was analyzed through the use of the radical species 2,2-diphenyl-l-picrylhydrazyl （DPPH） and zebrafish model, respectively. Quercetin-3-O-rutinoside （rutin） was the single principal phenolic compound. The extracts contained in vitro antioxidant activities and total phenolic contents （TPCs） between 1,143 ~ 112 lag and 4,633 ~ 174 lag gallic-acid equivalents （GAEs） per 100 g dry weight （DW）, though no relationship was found between the latter parameter and the antioxidant activity of the extracts. When zebrafish larvae were exposed to oxidative stress （2.4% v/v H2O2）, a concentration as low as 1.44 lag of GAEs/mL of piquillin-derived polyphenols inhibited lipid oxidation by up to 40%. Thus, in view of these advantageous functional food properties and the opportunity to exploit this Patagonian natural resource, piquillin consumption should be promoted worldwide.