Background： Plasmodium falciparum malaria remains a major life-threatening disease. Recently, the Plasmodium apoptosis-linked pathogenicity factors （PALPF） have been identified. These antigens PALPF are expressed o...Background： Plasmodium falciparum malaria remains a major life-threatening disease. Recently, the Plasmodium apoptosis-linked pathogenicity factors （PALPF） have been identified. These antigens PALPF are expressed only by P falciparum-infected erythrocytes triggering endothelial cell apoptosis （apoptogenic）. Methods： We designed ten synthetic peptides （PI to P10） from PALPF： PF07 0032, PF10_0226, PFI0130c, PFD0875c and MAL13P1.206, and analyzed their antigenicity with an ELISA method using plasma samples from subjects living in Dienga, Gabon. Results： Four peptides showed good reactivity with human antibodies. The prevalence rate of specific IgG was 61%, 51%, 44% and 34% for P5, P6, P4 and P2, respectively. The median optical density of total IgG anti-P2 was higher than that directed against P4 and P6 （P = 0.009; P = 0.012 respectively）. The prevalence rate oflgG subclasses determined with plasma samples recognizing peptide 5 for IgGl, 2, 3 and 4 isotypes was 69%, 45%, 76% and 62%, respectively. All the subjects had at least one immunoglobulin subclass, while 13 （44%） had both IgG1 and IgG3 antibodies. There was no significant difference in the prevalence rate of anti-P5 IgG1, IgG3 and IgG4. Conclusion： These results warrant further immunogenicity studies of peptides 2, 4, 5 and 6 with a view of a tentative to antimalarial vaccine development.展开更多
Background. Human herpesvirus-6 (HHV-6) infection is ubiquitous in selected population with sero-prevalence of 60-80%. Little is known for that in China, except few sporadic studies. To understand prevalence of HHV-6 ...Background. Human herpesvirus-6 (HHV-6) infection is ubiquitous in selected population with sero-prevalence of 60-80%. Little is known for that in China, except few sporadic studies. To understand prevalence of HHV-6 antibody in Chinese population,this seroepidemiological study was conducted.Methods. Sera were collected from 430 healthy persons and donors living in North China,and tested for HHV-6 antibodies using IFA with HHV-6 GS strain passaged on HSB-2 cells as antigen, and titer e-qual or higher than 1:10 was regarded as seropositive.Results. Of the 430 serum samples tested,297 (69.1%) were positive for HHV-6 IgG. Both seropositive rate and GMT in females were significantly higher than those in males,with X2 = 7. 05,P<0. 01 and F = 7.23,P<0. 01,respectively. Statistically significant difference in prevalence of HHV-6 antibody among various age groups was observed in both sexes,with X2=20. 08 and 20. 28,P = 0. 04,respectively,and young children had a higher titer than adults. But, no significant difference in prevalence was observed in blood donors between various age groups or both sexes.Conclusions. This study suggests that HHV-6 infection with seropositive IgG is ubiquitous in North China,and its importance should be further studied.展开更多
It has long been recognized that pedogeochemistry is closely related with human health. Based on analytical data, the weathering or soil forming processes fall into four stages and China is divided into four geochemic...It has long been recognized that pedogeochemistry is closely related with human health. Based on analytical data, the weathering or soil forming processes fall into four stages and China is divided into four geochemical regions: saline soil region, carbonate soil region, siallitic soil region and ferrallitic soil region. Moreover, some endemic diseases in relation to the deficit or excess of some elements are discussed in the viewpoint of pedogeochemistry.展开更多
AIM： To assess the association of measured gait speed with hemodialysis （HD） patients’ hospitalization, in conjunction with, and apart from, recent fall history.METHODS： Gait speed was measured by a standard prot...AIM： To assess the association of measured gait speed with hemodialysis （HD） patients’ hospitalization, in conjunction with, and apart from, recent fall history.METHODS： Gait speed was measured by a standard protocol and falls during the past 12 mo were ascertained for a prevalent multi-center HD cohort （n = 668） aged 20-92. Hospitalization during the past 12 mo was identified in the patient’s clinic records, and the first hospitalization after gait speed assessment （or the competing event of death） was identifed in the 2013 United States Renal Data System Standard Analysis Files.characterized 34.7% of the patients, and 27.1% had experienced a recent fall. Patients with slow gait speed but without a history of recent falls were 1.79 times more likely to have been hospitalized during the past 12 mo （OR = 1.79, 95%CI： 1.11-2.88, P = 0.02）, and patients with slow gait speed and a history of recent falls were over two times more likely to have been hospitalized （OR = 2.10, 95%CI： 1.19-3.73, P = 0.01）, compared with patients having faster gait speed and no recent fall history. Prospective examination of gait speed/fall history status in relation to frst hospitaliza-tion （or death） incurred by the end of follow-up Decem-ber 31, 2011 also showed that slow gait speed was as-sociated with these events in conjunction with a history of falls （HR = 1.54, 95%CI： 1.04-2.30, P = 0.03）.CONCLUSION： The International Task Force on Nutri-tion and Aging reported that gait speed is a powerful predictor for older adults of adverse outcomes such as hospitalization. In our data, gait speed-apart from, as well as in conjunction with, recent fall history-was as-sociated with HD patients’ hospitalization for multiple causes. Gait speed may be a sensitive health indicator among HD patients across the age spectrum.展开更多
The worldwide incidence and prevalence of cystic echinococcosis have fallen dramatically over the past several decades.Nonetheless,infection with Echinococcus granulosus(E.granulosus)remains a major public health issu...The worldwide incidence and prevalence of cystic echinococcosis have fallen dramatically over the past several decades.Nonetheless,infection with Echinococcus granulosus(E.granulosus)remains a major public health issue in several countries and regions,even in places where it was previously at low levels,as a result of a reduction of control programmes due to economic problems and lack of resources.Geographic distribution differs by country and region depending on the presence in that country of large numbers of nomadic or semi-nomadic sheep and goat flocks that represent the intermediate host of the parasite,and their close contact with the final host,the dog,which mostly provides the transmission of infection to humans.The greatest prevalence of cystic echinococcosis in human and animal hosts is found in countries of the temperate zones,including several parts of Eurasia(the Mediterranean regions,southern and central parts of Russia,central Asia,China),Australia,some parts of America(especially South America)and north and east Africa.Echinococcosis is currently considered an endemic zoonotic disease in the Mediterranean region.The most frequent strain associated with human cystic echinococcosis appears to be the common sheep strain(G1).This strain appears to be widely distributed in all continents.The purpose of this review is to examine the distribution of E.granulosus and the epidemiology of a re-emerging disease such as cystic echinococcosis.展开更多
Developing countries suffer the highest burden of cervical cancers but have the lowest resources. Effective cervical cytology screening programme, along with a network of diagnostic and therapeutic colposcopy centres,...Developing countries suffer the highest burden of cervical cancers but have the lowest resources. Effective cervical cytology screening programme, along with a network of diagnostic and therapeutic colposcopy centres, like developed countries, is almost impossible to be reproduced in developing countries. Visual inspection methods [e.g., Visual inspection with Lugol's iodine(VILI) and Visual Inspection with Acetic Acid(VIA)] which are cheaper, require less expertise and have the advantage of possible treatment in one setting have been shown to be effective alternatives. The sensitivity to detect CIN2+, by VIA and VILI, have been shown to be 80% and 91% respectively, with a specificity rate of 92% and 85% respectively. Screening by human papillomavirus(HPV) testing has high sensitivity(96.4%) but low specificity(94.1%) to detect CIN2+, when compared to Pap Smear(sensitivity, 55.4% and specificity, 96.8%). A single lifetime HPV testing in a large unscreened population has been shown to significantlyreduce cervical cancer incidence and mortality when compared to cervical cytology, VIA or no screening. HPV testing of self-collected vaginal specimens also helps to overcome religious and socio-cultural barriers towards pelvic examination amongst women in developing countries. Current HPV testing methods are expensive, skill/infrastructure demanding and takes time to produce results. A cheaper HPV test, called careH PV?, which is able to provide results within 2.5 h and requires minimal skill/infrastructure to operate, was designed for use in developing countries. One stop screen and treat facilities using VIA or rapid HPV testing, and cryotherapy, can overcome non-compliance to follow-up which is a major issue in developing countries. Cure rates of 81.4% for CIN1, 71.4% for CIN2 and 68.0% for CIN3 at 6 mo after treatment have been reported. Incorporating telemedicine with cervicography of VIA or VILI or even telecolposcopy, has great potential in cervical cancer screening, especially in countries with vast geographical areas.展开更多
OBJECTIVE To investigate the detection rate of humanpapilloma virus (HPV) DNA in the Kazakh esophageal carcinoma(EC) patients of Xinjiang.METHODS We detected the prevalence of a HPV gene in tumortissues from 318 esoph...OBJECTIVE To investigate the detection rate of humanpapilloma virus (HPV) DNA in the Kazakh esophageal carcinoma(EC) patients of Xinjiang.METHODS We detected the prevalence of a HPV gene in tumortissues from 318 esophageal squamous cell carcinoma (ESCC).Tumor tissues were kept in formalin and embedded in paraffin.One hundred seventeen samples used crude cell suspension, whilethe other 201 used the method of DNA extraction with phenol-Tris/chloroform. We analyzed the relevance to EC of Kazakh's inXinjiang.RESULTS In the ESCC samples of Kazakh's in Xinjiang, totaldetection rate for HPV DNA was 64.5% (205/318). The positiverate of HPV in group of crude cell suspensions was 82.9% (97/117)compared with the rate of 53.7% (108/201) in the group of DNAextraction. The results in the two groups showed significantdiffference (x^2 = 5.711, P < 0.05).CONCLUSION HPV DNA infection may be one of the mostimportant factors related to EC of Kazakh's in Xinjiang.展开更多
Objective： To study the factors affecting extracellular glycerol （Gly） in patients with severe traumatic brain injury （STBI）. Methods： Perilesional extracellular Gly and cerebral blood flow （CBF） in 53 patien...Objective： To study the factors affecting extracellular glycerol （Gly） in patients with severe traumatic brain injury （STBI）. Methods： Perilesional extracellular Gly and cerebral blood flow （CBF） in 53 patients with STBI were consecutively monitored. Simultaneously, the intracranial pressure （ICP） and cerebral perfusion pressure （CCP） were monitored. The hourly minimum of CCP and CBF and the hourly maximum of ICP levels were matched with the hourly Gly. Gly values were divided into several groups according to regional ICP （〈 15 nun Hg or 〉 15 nun Hg）, CCP （〈70 nun Hg or 〉70 nun Hg）, CBF （〈50 AU or 50-150 AU） and the outcomes （death or persistent vegetative state group, severe or moderate disability group, and good recovery group）. Results： In comparison with the severe or moderate disability group, the Gly concentration of the death or persistent vegetative state group increased significantly, but CBF and CCP decreased significantly. In comparison with the good recovery group, the Gly concentration of the severe or moderate disability group increased significantly, but CBF and CCP decreased significantly. The Gly concen- trations in patients with ICP〉15 mm Hg, CCP〈70 mm Hg and CBF〈50 AU were respectively higher than those of patients with ICP 〈15 mm Hg, CCP〉70 mm Hg and 50AU 〈CBF〈150AU. In patients with diffuse axial injury, the mean Gly concentration was （201.17±55.00） μmol/L, which was significantly higher than that of the patients with epidural hematoma （n=7, 73.26±8.37, P〈O.05） or subdural hematoma （n=9, 114.67 ±62.88, P〈O.05）, but it did not increase signifi- cantly when compared with those in patients with contusion （n=24, 167.48±52.63）. Conclusion： Gly can be taken as a marker for degrada- tion of membrane phospholipids and ischemia, which reflects the severity of primary or secondary insult.展开更多
文摘Background： Plasmodium falciparum malaria remains a major life-threatening disease. Recently, the Plasmodium apoptosis-linked pathogenicity factors （PALPF） have been identified. These antigens PALPF are expressed only by P falciparum-infected erythrocytes triggering endothelial cell apoptosis （apoptogenic）. Methods： We designed ten synthetic peptides （PI to P10） from PALPF： PF07 0032, PF10_0226, PFI0130c, PFD0875c and MAL13P1.206, and analyzed their antigenicity with an ELISA method using plasma samples from subjects living in Dienga, Gabon. Results： Four peptides showed good reactivity with human antibodies. The prevalence rate of specific IgG was 61%, 51%, 44% and 34% for P5, P6, P4 and P2, respectively. The median optical density of total IgG anti-P2 was higher than that directed against P4 and P6 （P = 0.009; P = 0.012 respectively）. The prevalence rate oflgG subclasses determined with plasma samples recognizing peptide 5 for IgGl, 2, 3 and 4 isotypes was 69%, 45%, 76% and 62%, respectively. All the subjects had at least one immunoglobulin subclass, while 13 （44%） had both IgG1 and IgG3 antibodies. There was no significant difference in the prevalence rate of anti-P5 IgG1, IgG3 and IgG4. Conclusion： These results warrant further immunogenicity studies of peptides 2, 4, 5 and 6 with a view of a tentative to antimalarial vaccine development.
文摘Background. Human herpesvirus-6 (HHV-6) infection is ubiquitous in selected population with sero-prevalence of 60-80%. Little is known for that in China, except few sporadic studies. To understand prevalence of HHV-6 antibody in Chinese population,this seroepidemiological study was conducted.Methods. Sera were collected from 430 healthy persons and donors living in North China,and tested for HHV-6 antibodies using IFA with HHV-6 GS strain passaged on HSB-2 cells as antigen, and titer e-qual or higher than 1:10 was regarded as seropositive.Results. Of the 430 serum samples tested,297 (69.1%) were positive for HHV-6 IgG. Both seropositive rate and GMT in females were significantly higher than those in males,with X2 = 7. 05,P<0. 01 and F = 7.23,P<0. 01,respectively. Statistically significant difference in prevalence of HHV-6 antibody among various age groups was observed in both sexes,with X2=20. 08 and 20. 28,P = 0. 04,respectively,and young children had a higher titer than adults. But, no significant difference in prevalence was observed in blood donors between various age groups or both sexes.Conclusions. This study suggests that HHV-6 infection with seropositive IgG is ubiquitous in North China,and its importance should be further studied.
文摘It has long been recognized that pedogeochemistry is closely related with human health. Based on analytical data, the weathering or soil forming processes fall into four stages and China is divided into four geochemical regions: saline soil region, carbonate soil region, siallitic soil region and ferrallitic soil region. Moreover, some endemic diseases in relation to the deficit or excess of some elements are discussed in the viewpoint of pedogeochemistry.
基金Supported by National Institutes of Health contract HHSN267200715004C,ADB No.N01-DK-7-5004(Dr.Kutner)
文摘AIM： To assess the association of measured gait speed with hemodialysis （HD） patients’ hospitalization, in conjunction with, and apart from, recent fall history.METHODS： Gait speed was measured by a standard protocol and falls during the past 12 mo were ascertained for a prevalent multi-center HD cohort （n = 668） aged 20-92. Hospitalization during the past 12 mo was identified in the patient’s clinic records, and the first hospitalization after gait speed assessment （or the competing event of death） was identifed in the 2013 United States Renal Data System Standard Analysis Files.characterized 34.7% of the patients, and 27.1% had experienced a recent fall. Patients with slow gait speed but without a history of recent falls were 1.79 times more likely to have been hospitalized during the past 12 mo （OR = 1.79, 95%CI： 1.11-2.88, P = 0.02）, and patients with slow gait speed and a history of recent falls were over two times more likely to have been hospitalized （OR = 2.10, 95%CI： 1.19-3.73, P = 0.01）, compared with patients having faster gait speed and no recent fall history. Prospective examination of gait speed/fall history status in relation to frst hospitaliza-tion （or death） incurred by the end of follow-up Decem-ber 31, 2011 also showed that slow gait speed was as-sociated with these events in conjunction with a history of falls （HR = 1.54, 95%CI： 1.04-2.30, P = 0.03）.CONCLUSION： The International Task Force on Nutri-tion and Aging reported that gait speed is a powerful predictor for older adults of adverse outcomes such as hospitalization. In our data, gait speed-apart from, as well as in conjunction with, recent fall history-was as-sociated with HD patients’ hospitalization for multiple causes. Gait speed may be a sensitive health indicator among HD patients across the age spectrum.
文摘The worldwide incidence and prevalence of cystic echinococcosis have fallen dramatically over the past several decades.Nonetheless,infection with Echinococcus granulosus(E.granulosus)remains a major public health issue in several countries and regions,even in places where it was previously at low levels,as a result of a reduction of control programmes due to economic problems and lack of resources.Geographic distribution differs by country and region depending on the presence in that country of large numbers of nomadic or semi-nomadic sheep and goat flocks that represent the intermediate host of the parasite,and their close contact with the final host,the dog,which mostly provides the transmission of infection to humans.The greatest prevalence of cystic echinococcosis in human and animal hosts is found in countries of the temperate zones,including several parts of Eurasia(the Mediterranean regions,southern and central parts of Russia,central Asia,China),Australia,some parts of America(especially South America)and north and east Africa.Echinococcosis is currently considered an endemic zoonotic disease in the Mediterranean region.The most frequent strain associated with human cystic echinococcosis appears to be the common sheep strain(G1).This strain appears to be widely distributed in all continents.The purpose of this review is to examine the distribution of E.granulosus and the epidemiology of a re-emerging disease such as cystic echinococcosis.
基金Supported by fellowships from the Prime Minister of AustraliaAsia Endeavour Award,Ministry of Higher Education,Malaysiaand University Technologi MARA,Malaysia
文摘Developing countries suffer the highest burden of cervical cancers but have the lowest resources. Effective cervical cytology screening programme, along with a network of diagnostic and therapeutic colposcopy centres, like developed countries, is almost impossible to be reproduced in developing countries. Visual inspection methods [e.g., Visual inspection with Lugol's iodine(VILI) and Visual Inspection with Acetic Acid(VIA)] which are cheaper, require less expertise and have the advantage of possible treatment in one setting have been shown to be effective alternatives. The sensitivity to detect CIN2+, by VIA and VILI, have been shown to be 80% and 91% respectively, with a specificity rate of 92% and 85% respectively. Screening by human papillomavirus(HPV) testing has high sensitivity(96.4%) but low specificity(94.1%) to detect CIN2+, when compared to Pap Smear(sensitivity, 55.4% and specificity, 96.8%). A single lifetime HPV testing in a large unscreened population has been shown to significantlyreduce cervical cancer incidence and mortality when compared to cervical cytology, VIA or no screening. HPV testing of self-collected vaginal specimens also helps to overcome religious and socio-cultural barriers towards pelvic examination amongst women in developing countries. Current HPV testing methods are expensive, skill/infrastructure demanding and takes time to produce results. A cheaper HPV test, called careH PV?, which is able to provide results within 2.5 h and requires minimal skill/infrastructure to operate, was designed for use in developing countries. One stop screen and treat facilities using VIA or rapid HPV testing, and cryotherapy, can overcome non-compliance to follow-up which is a major issue in developing countries. Cure rates of 81.4% for CIN1, 71.4% for CIN2 and 68.0% for CIN3 at 6 mo after treatment have been reported. Incorporating telemedicine with cervicography of VIA or VILI or even telecolposcopy, has great potential in cervical cancer screening, especially in countries with vast geographical areas.
基金supported by grants from State Key Development Program of Basic Research of China(No.2005CCA03700/2007CB516804)Science Foundation of Ministry of Education of China(No.206167)National Natural Science Foundation of China(No.30660161).
文摘OBJECTIVE To investigate the detection rate of humanpapilloma virus (HPV) DNA in the Kazakh esophageal carcinoma(EC) patients of Xinjiang.METHODS We detected the prevalence of a HPV gene in tumortissues from 318 esophageal squamous cell carcinoma (ESCC).Tumor tissues were kept in formalin and embedded in paraffin.One hundred seventeen samples used crude cell suspension, whilethe other 201 used the method of DNA extraction with phenol-Tris/chloroform. We analyzed the relevance to EC of Kazakh's inXinjiang.RESULTS In the ESCC samples of Kazakh's in Xinjiang, totaldetection rate for HPV DNA was 64.5% (205/318). The positiverate of HPV in group of crude cell suspensions was 82.9% (97/117)compared with the rate of 53.7% (108/201) in the group of DNAextraction. The results in the two groups showed significantdiffference (x^2 = 5.711, P < 0.05).CONCLUSION HPV DNA infection may be one of the mostimportant factors related to EC of Kazakh's in Xinjiang.
文摘Objective： To study the factors affecting extracellular glycerol （Gly） in patients with severe traumatic brain injury （STBI）. Methods： Perilesional extracellular Gly and cerebral blood flow （CBF） in 53 patients with STBI were consecutively monitored. Simultaneously, the intracranial pressure （ICP） and cerebral perfusion pressure （CCP） were monitored. The hourly minimum of CCP and CBF and the hourly maximum of ICP levels were matched with the hourly Gly. Gly values were divided into several groups according to regional ICP （〈 15 nun Hg or 〉 15 nun Hg）, CCP （〈70 nun Hg or 〉70 nun Hg）, CBF （〈50 AU or 50-150 AU） and the outcomes （death or persistent vegetative state group, severe or moderate disability group, and good recovery group）. Results： In comparison with the severe or moderate disability group, the Gly concentration of the death or persistent vegetative state group increased significantly, but CBF and CCP decreased significantly. In comparison with the good recovery group, the Gly concentration of the severe or moderate disability group increased significantly, but CBF and CCP decreased significantly. The Gly concen- trations in patients with ICP〉15 mm Hg, CCP〈70 mm Hg and CBF〈50 AU were respectively higher than those of patients with ICP 〈15 mm Hg, CCP〉70 mm Hg and 50AU 〈CBF〈150AU. In patients with diffuse axial injury, the mean Gly concentration was （201.17±55.00） μmol/L, which was significantly higher than that of the patients with epidural hematoma （n=7, 73.26±8.37, P〈O.05） or subdural hematoma （n=9, 114.67 ±62.88, P〈O.05）, but it did not increase signifi- cantly when compared with those in patients with contusion （n=24, 167.48±52.63）. Conclusion： Gly can be taken as a marker for degrada- tion of membrane phospholipids and ischemia, which reflects the severity of primary or secondary insult.
