[Objective] This study aimed to investigate the polymorphism of PBF en- coding genes from common wheat Chinese Spring (Triticum aestivum L.). [Method] Using common wheat Chinese Spring as the experimental material, ...[Objective] This study aimed to investigate the polymorphism of PBF en- coding genes from common wheat Chinese Spring (Triticum aestivum L.). [Method] Using common wheat Chinese Spring as the experimental material, gene-specific primers were designed and applied to amplify the genomic DNA of Chinese Spring. PCR products were isolated, purified and ligated into the cloning vector. Positive clones were randomly selected for sequencing. A series of softwares including DNAMAN, Signalp, PSIPRED, Nuc_PLoc and MEGA were employed for sequence assembly and alignment, signal peptide prediction, primary and secondary structure prediction, as well as analyses of subcellular location and phylogenetic relationships between the PBF family members in Poaceae. [Result] Twenty-five target sequences were obtained from the genome of hexaploid common wheat Chinese Spring, which were classified into three clusters based on the sequence similarity. SNPs exist at two loci of the subunit, resulting in the change of encoded amino acid residues and affecting the secondary structure of final product encoded. [Conclusion] PBF encoding sequences are extremely conservative in Chinese Spring with certain variations. This study provides theoretical reference to evaluate the expression efficiency of wheat storage proteins.展开更多
We propose a scheme to implement the optimal symmetric 1 → 2 universal quantum telecloning through cavity-assisted interaction. In our scheme an arbitrary single atomic state can be telecloned to two single atomic st...We propose a scheme to implement the optimal symmetric 1 → 2 universal quantum telecloning through cavity-assisted interaction. In our scheme an arbitrary single atomic state can be telecloned to two single atomic states. And three atoms are trapped in three spatially separated cavities respectively. With a particular multiparticle entangled state acting as a quantum information channel and the trapped single atom acting as a quantum network node for its long-lived internal state, quantum information can be telecloned among nodes and can stored in the nodes.展开更多
基金Supported by National Natural Science Foundation of China(30900896)Special Fund for the Construction of Modern Agricultural Technology System(NYCYTX-001)Cyrus Tang Breeding Fund(A212020912)~~
文摘[Objective] This study aimed to investigate the polymorphism of PBF en- coding genes from common wheat Chinese Spring (Triticum aestivum L.). [Method] Using common wheat Chinese Spring as the experimental material, gene-specific primers were designed and applied to amplify the genomic DNA of Chinese Spring. PCR products were isolated, purified and ligated into the cloning vector. Positive clones were randomly selected for sequencing. A series of softwares including DNAMAN, Signalp, PSIPRED, Nuc_PLoc and MEGA were employed for sequence assembly and alignment, signal peptide prediction, primary and secondary structure prediction, as well as analyses of subcellular location and phylogenetic relationships between the PBF family members in Poaceae. [Result] Twenty-five target sequences were obtained from the genome of hexaploid common wheat Chinese Spring, which were classified into three clusters based on the sequence similarity. SNPs exist at two loci of the subunit, resulting in the change of encoded amino acid residues and affecting the secondary structure of final product encoded. [Conclusion] PBF encoding sequences are extremely conservative in Chinese Spring with certain variations. This study provides theoretical reference to evaluate the expression efficiency of wheat storage proteins.
基金supported by National Natural Science Foundation of China under Grant No.10674001the Program of the Education Department of Anhui Province under Grant No.KJ2007A002the Youth Program of Fu Yang Normal College under Grant No.2008LQ04
文摘We propose a scheme to implement the optimal symmetric 1 → 2 universal quantum telecloning through cavity-assisted interaction. In our scheme an arbitrary single atomic state can be telecloned to two single atomic states. And three atoms are trapped in three spatially separated cavities respectively. With a particular multiparticle entangled state acting as a quantum information channel and the trapped single atom acting as a quantum network node for its long-lived internal state, quantum information can be telecloned among nodes and can stored in the nodes.