Aim To establish an RP-HPLC method for simultaneous determination of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract. Methods Chromatography was carfled out on Kromasil C18 column （250 mm...Aim To establish an RP-HPLC method for simultaneous determination of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract. Methods Chromatography was carfled out on Kromasil C18 column （250 mm × 4.6 mm, 5 μm）, using THF-CH3CN-H2O-H3PO4 （30 ： 5： 125 ： 0. 1） as the mobile phase at a flow rate of 1.0 mL·min^-1. The UV detection wavelength was 270 nm. Results The linear range of 2＂-O-rhamnosyl vitexin and vitexin were 0. 106 4 μg - 2. 1280 μg （ r =0. 999 1 ） and 0. 139 2μg - 2. 784 0 μg （ r =0. 999 3 ）, respectively. The average recoveries of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf were 99.2% （ RSD = 2.80%, n = 6） and 100.6% （ RSD = 2.84%, n = 6）, respectively. Conclusion This method is reproducible, simple, precise, and rapid for simultaneous determination of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract, thereby providinge the basis for quality specification of Chinese hawthorn leaf and its extract.展开更多
[Objective] Fermentation process of hawthorn vinegar was investigated in order to explore the effect of process andobtain superior process of hawthorn vine- gar. [Method] Fermentation process of hawthorn vinegar was f...[Objective] Fermentation process of hawthorn vinegar was investigated in order to explore the effect of process andobtain superior process of hawthorn vine- gar. [Method] Fermentation process of hawthorn vinegar was firstly optimized using Box-Behnken methodologyof 3-factor and 3-level, and the effect of process was an- alyzed. Secondly, it was also finished to build secondary multivariate regression model related to yield of acetic acid. [Results] The optimal fermentation process ob- tained by response surface methodology was as follows： inoculum size of 10%, or- iginal alcohol content of 7.0% and temperature of 30 ^（3. In optimal condition, yield of acetic acid was 51.76 g/L which was close to predicted value （51.77 g/L）. In the model, interaction of inoculum size and original alcohol content was extremely signif- icant. In contrast, interaction of others was non-significant. [Conclusion] Response surface methodology is advisable to be used for process optimization of hawthorn vinegar.展开更多
[Objective] The aim was to explore the most efficient medium for pollen germination and suitable pollen storage temperature for three different hawthorn species in Xinjiang, and to lay foundation for the development a...[Objective] The aim was to explore the most efficient medium for pollen germination and suitable pollen storage temperature for three different hawthorn species in Xinjiang, and to lay foundation for the development and utilization of wild hawthorn resources. [Method] A randomized block design was adopted to select the most suitable concentrations of sucrose and boric acid in the medium for pollen germination under in vitro culture. The vitality of the pollens stored at different tem- peratures for six mouths was measured once every 10 d using the optimum medi- um selected in above steps. [Result] The most efficient medium for Altai hawthorn pollen germination was 1% agar ＋ 0.05% boric acid ＋ 15% sucrose, and its germi- nation rate in this medium was 30.8%; the most suitable medium for crabapple hawthorn pollen germination was 1% agar ＋ 0.01% boric acid ＋ 15% sucrose, and its germination rate in this medium was 58.7%; and the highest germination rate of Junggar hawthorn pollen was found in the medium containing 1% agar ＋ 0.01% boric acid ＋ 20% sucrose, and the germination rate reached 67.2%. There were extremely significant differences （P〈0.01） in germination rate between these treat- ments and others in each species. The pollen vitality of Altai hawthorn, crabapple hawthorn and Junggar hawthorn were lost completely after they were stored at room temperature for 40, 60 and 70 d; at 4-5 ℃ for 90, 130, and 140 d; or at -18 ℃ for 130, 160 and 170 d. Therefore, -18 ℃ was found the most efficient storage temperature for the three hawthorn species＇ pollen among the three different stor- age temperatures. [Conclusion] A certain concentration of sucrose and boric acid can promote pollen germination of the three hawthorn species. Under different stor- age temperatures, the pollen vitality of the three hawthorn species can maintain for a longer period at -18 ℃ than at 4-5 ℃, and for the shortest period at room tem- perature.展开更多
[Objective] This study was conducted to optimize PCR-SSCP reaction system and conditions for hawthorn （Crataegus spp.）. [Method] The chloroplast DNA and nuclear DNA of hawthorn leaf were extracted with improved CTAB...[Objective] This study was conducted to optimize PCR-SSCP reaction system and conditions for hawthorn （Crataegus spp.）. [Method] The chloroplast DNA and nuclear DNA of hawthorn leaf were extracted with improved CTAB method. Pdmers for the PCR amplification of chloroplast DNA and nuclear DNA were select- ed from eight pairs of candidate pdmers, and the PCR-SSCP reaction system and reaction conditions were optimized. The PCR products were detected by agarose gel electrophoresis, and the denatured PCR-SSCP products were analyzed by native polyacrylamide gel. [Result] Five pairs primers （psbA-tmH, ropB, ropL, rpoC1 and ITS2） were proved to be suitable for PCR-SSCP in hawthorn, including four for chloroplast DNA and one for nuclear DNA. The clear electrophoretogram of PCR- SSCP in hawthorn was obtained by performing electrophoresis in 0.5×TBE buffer, at 4 ℃ and 200 V for 3-4 h, using 6% native polyacrylamide gel （crosslinking ratio at 29：1）, and the PCR product had been mixed with an equal volume of loading buffer containing 1% NaOH （without glycerol） and denatured at 98℃ for 15 min. [Conclu- sion] The results may lay the foundation of SSCP analysis of hawthom.展开更多
文摘Aim To establish an RP-HPLC method for simultaneous determination of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract. Methods Chromatography was carfled out on Kromasil C18 column （250 mm × 4.6 mm, 5 μm）, using THF-CH3CN-H2O-H3PO4 （30 ： 5： 125 ： 0. 1） as the mobile phase at a flow rate of 1.0 mL·min^-1. The UV detection wavelength was 270 nm. Results The linear range of 2＂-O-rhamnosyl vitexin and vitexin were 0. 106 4 μg - 2. 1280 μg （ r =0. 999 1 ） and 0. 139 2μg - 2. 784 0 μg （ r =0. 999 3 ）, respectively. The average recoveries of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf were 99.2% （ RSD = 2.80%, n = 6） and 100.6% （ RSD = 2.84%, n = 6）, respectively. Conclusion This method is reproducible, simple, precise, and rapid for simultaneous determination of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract, thereby providinge the basis for quality specification of Chinese hawthorn leaf and its extract.
基金Supported by Science and Technology Project Program of Guangdong Province(No.2014B040404027)~~
文摘[Objective] Fermentation process of hawthorn vinegar was investigated in order to explore the effect of process andobtain superior process of hawthorn vine- gar. [Method] Fermentation process of hawthorn vinegar was firstly optimized using Box-Behnken methodologyof 3-factor and 3-level, and the effect of process was an- alyzed. Secondly, it was also finished to build secondary multivariate regression model related to yield of acetic acid. [Results] The optimal fermentation process ob- tained by response surface methodology was as follows： inoculum size of 10%, or- iginal alcohol content of 7.0% and temperature of 30 ^（3. In optimal condition, yield of acetic acid was 51.76 g/L which was close to predicted value （51.77 g/L）. In the model, interaction of inoculum size and original alcohol content was extremely signif- icant. In contrast, interaction of others was non-significant. [Conclusion] Response surface methodology is advisable to be used for process optimization of hawthorn vinegar.
基金Supported by Special Fund for Forestry-scientific Research in the Public Interest(201404722)Xinjiang Uygur Autonomous Region Fruit Trees Key Subject~~
文摘[Objective] The aim was to explore the most efficient medium for pollen germination and suitable pollen storage temperature for three different hawthorn species in Xinjiang, and to lay foundation for the development and utilization of wild hawthorn resources. [Method] A randomized block design was adopted to select the most suitable concentrations of sucrose and boric acid in the medium for pollen germination under in vitro culture. The vitality of the pollens stored at different tem- peratures for six mouths was measured once every 10 d using the optimum medi- um selected in above steps. [Result] The most efficient medium for Altai hawthorn pollen germination was 1% agar ＋ 0.05% boric acid ＋ 15% sucrose, and its germi- nation rate in this medium was 30.8%; the most suitable medium for crabapple hawthorn pollen germination was 1% agar ＋ 0.01% boric acid ＋ 15% sucrose, and its germination rate in this medium was 58.7%; and the highest germination rate of Junggar hawthorn pollen was found in the medium containing 1% agar ＋ 0.01% boric acid ＋ 20% sucrose, and the germination rate reached 67.2%. There were extremely significant differences （P〈0.01） in germination rate between these treat- ments and others in each species. The pollen vitality of Altai hawthorn, crabapple hawthorn and Junggar hawthorn were lost completely after they were stored at room temperature for 40, 60 and 70 d; at 4-5 ℃ for 90, 130, and 140 d; or at -18 ℃ for 130, 160 and 170 d. Therefore, -18 ℃ was found the most efficient storage temperature for the three hawthorn species＇ pollen among the three different stor- age temperatures. [Conclusion] A certain concentration of sucrose and boric acid can promote pollen germination of the three hawthorn species. Under different stor- age temperatures, the pollen vitality of the three hawthorn species can maintain for a longer period at -18 ℃ than at 4-5 ℃, and for the shortest period at room tem- perature.
文摘[Objective] This study was conducted to optimize PCR-SSCP reaction system and conditions for hawthorn （Crataegus spp.）. [Method] The chloroplast DNA and nuclear DNA of hawthorn leaf were extracted with improved CTAB method. Pdmers for the PCR amplification of chloroplast DNA and nuclear DNA were select- ed from eight pairs of candidate pdmers, and the PCR-SSCP reaction system and reaction conditions were optimized. The PCR products were detected by agarose gel electrophoresis, and the denatured PCR-SSCP products were analyzed by native polyacrylamide gel. [Result] Five pairs primers （psbA-tmH, ropB, ropL, rpoC1 and ITS2） were proved to be suitable for PCR-SSCP in hawthorn, including four for chloroplast DNA and one for nuclear DNA. The clear electrophoretogram of PCR- SSCP in hawthorn was obtained by performing electrophoresis in 0.5×TBE buffer, at 4 ℃ and 200 V for 3-4 h, using 6% native polyacrylamide gel （crosslinking ratio at 29：1）, and the PCR product had been mixed with an equal volume of loading buffer containing 1% NaOH （without glycerol） and denatured at 98℃ for 15 min. [Conclu- sion] The results may lay the foundation of SSCP analysis of hawthom.
