论水利工程建设与水利风景区建设的统一性

本文首先是对水利工程以及水利风景区在建设方面的概念进行简要阐述,分析水利风景区目前在发展中存在的问题。表明通过以水利工程为依托的水利风景区建设打造“水+”融合发展体系,从而发挥出水利的最大优势,最终有效实现水利的生态价值与社会价值。

Embryological Studies on Facultative Apomixis in Apluda mutica (Gramineae)

A cytological study on Apluda mutica L. revealed that this species was a facultative apomict with 60.74% of apomictic embryo sac formation. Its sexual reproduction exhibited the characteristics of polygonum_type embryo sac formation. After the 3 megaspores at the micropylar end had degenerated, leaving only the chalazal megaspore in the nucellus, a nucellar cell adjacent to the megaspore differentiated into an aposporous initial, which later developed into a panicum_type embryo sac. It was an aposporous 4_nucleate embryo sac without chromosome reduction and composed of an egg, a single synergid and a central cell with 2 polar nuclei. The chalazal megaspore degenerated before the onset of the division in the aposporous initial cell.

Evaluation of CMPAS precipitation products over Sichuan,China

High-quality and high-resolution precipitation data are the basis for mesoscale numerical weather forecasting,model verification,and hydrological monitoring,which play an important role in meteorological and hydrological disaster prevention and mitigation.In this study,high-density rain gauge data are used to evaluate the fusion accuracy of the China Meteorological Administration Multisource Precipitation Analysis System(CMPAS),and four CMPAS products with different spatial and temporal resolution and different data sources are compared,to derive the applicability of CMPAS.Results show that all the CMPAS products show high accuracy in the Sichuan Basin,followed by Panxi Area and the western Sichuan Plateau.The errors of the four products all rise with the increase in precipitation.CMPAS overestimates precipitation in summer and autumn and underestimates it in spring and winter.Overall,the applicability of these fused data in the Sichuan Basin is quite good.Due to the lack of observations and the influence of the terrain and meteorological conditions,the evaluation of CMPAS in the plateau area needs further analysis.

Research on the Strategy of Underwater United Detection Fusion and Communication Using Multi-sensor

In order to solve the distributed detection fusion problem of underwater target detection, when the signal to noise ratio (SNR) of the acoustic channel is low, a new strategy for united detection fusion and communication using multiple sensors was proposed. The performance of detection fusion was studied and compared based on the Neyman-Pearson principle when the binary phase shift keying (BPSK) and on-off keying (OOK) modes were used by the local sensors. The comparative simulation and analysis between the optimal likelihood ratio test and the proposed strategy was completed, and both the theoretical analysis and simulation indicate that using the proposed new strategy could improve the detection performance effectively. In theory, the proposed strategy of united detection fusion and communication is of great significance to the establishment of an underwater target detection system.

Physical Simulation of Water Dissolved Gas (WDG)During Migration

By using the equipment designed and developed by ourselves, experiment of investigating the influence of dissolution on some geo-chemical parameters (such as δ13C,δD,and iC4/nC4 in water-dissolved gas (WDG) during migration) was performed. The result shows that, with the increase of distance, 1) the relative abundance of non-hydrocarbon (CO2) and hydrocarbon (CH4) increase while the relative abundance of hydrocarbon (C2+) decreases (the relative abundance of hydrocarbon (C5+) can be basically negligible); 2) the relative abundance of benzene and methylbenzene increase in the initial time and then decrease. The carbon and hydrogen isotopes of methane vary slightly, which can be regarded as indicators of gas dissolved in water formation.

Surface Display of Rice Stripe Virus NSvc2 and Analysis of Its Membrane Fusion Activity

Rice stripe virus (RSV) infects rice and is transmitted in a propagative manner by the small brown planthopper. How RSV enters an insect cell to initiate the infection cycle is poorly understood. Sequence analysis revealed that the RSV NSvc2 protein was similar to the membrane glycoproteins of several members in the family Bunyaviridae and might induce cell membrane fusion. To conveniently study the membrane fusion activity of NSvc2, we constructed cell surface display vectors for expressing Nsvc2 on the insect cell surface as the membrane glycoproteins of the enveloped viruses. Our results showed that NSvc2 was successfully expressed and displayed on the surface of insect Sf9 cells. When induced by low pH, the membrane fusion was not observed in the cells that expressed NSvc2. Additionally, the membrane fusion was also not detected when co-expressing Nsvc2 and the viral capsid protein on insect cell surface. Thus, RSV NSvc2 is probably different from the phlebovirus counterparts, which could suggest different functions. RSV might enter insect cells other than by fusion with plasma or endosome membrane.

Expression of Overlapping PCR-generated Shiga Toxin B Gene Fragment in E. Coli and Its Ascitic Polyclonal Antibody

The mature Shiga toxin B （StxB） gene was optimized and generated by overlapping PCR. Recombinant expression vector pQE40-DHFR/StxB was constructed when the gene was cloned into pQE fusion expression vector. Induced by Isopropyl β-D-thiogalactoside （IPTG）, the DHFR/StxB fusion protein was highly expressed to the level of 41.36% in E. coli MI5 cells. The 35 kDa fusion protein with a 6 His-tag was one-step purified from inclusion bodies using Ni-NTA affinity chromatography column under denaturing conditions, and was refolded by dialyzing with a decreasing urea gradient. Purified DHFR/StxB fusion protein was used to immunize Kunming mice for generating the ascitic polyclonal antibody against recombinant StxB protein by injecting sarcoma 180 cells and the titer ofascitic polyclonal antibody is up to 1： 1× 10^6 detected by the indirect enzyme linked immunosorbent assy （ELISA）. Western immunoblotting analysis revealed that the ascitic polyclonal antibody against StxB had a specific affinity for a 70 kDa shiga toxin protein of Shigella dysenteriae type 1. It is a new simple and quick method to produce a large amount of ascitic polyclonal antibody. The antibody is used to develop immunological method for detecting shiga toxin.