The paper reviewed the function mechanism of Bilken virusicide against rice stipe disease, and then introduced its control effects in field test as well as its application method.
[Objective] The efficacy of difenoconazole-azoxystrobin 32.5% SC for rice sheath blight and its application technology were discussed in this research. [Method] Three surveys were carried out. There were 5 fixed test ...[Objective] The efficacy of difenoconazole-azoxystrobin 32.5% SC for rice sheath blight and its application technology were discussed in this research. [Method] Three surveys were carried out. There were 5 fixed test clumps in each plot. The number of total plants and disease plants and disease progression in each fixed clump were recorded. The correlation effectiveness was calculated based on the growth rate of disease index. Significance analysis was performed with Dun- can＇s new multiple range method （DMRT）. [Result] The difenoconazole-azoxystrobin 32.5% SC had a good efficacy for rice sheath blight, and its efficacy increased with the increase of dose. If sprayed according to the dose of 450 ml/hm2 5 days before the beginning of heading stage of rice, difenoconazole-azoxystrobin 32.5% SC would have better effects on controlling rice sheath blight with correlation effectiveness reaching up to 95.14%, which was 2.06% higher than that of control drug （trifloxys- trobin-tebuconazole 75% WG, 225 g/hm2）. [Conclusion] Treated with difenoconazole- azoxystrobin 32.5% SC with dose of 450 ml/hm2, rice would have green upper leaves and less yellow middle and lower leaves. Moreover, the yield was in- creased significantly. The difenoconazole-azoxystrobin 32.5% SC had good safety. Therefore, difenoconazole-azoxystrobin 32.5% SC had a good application prospect in production.展开更多
Previous studies indicated that fruit bats carry two betacoronaviruses,BatCoV HKU9 and BatCoV GCCDC1.To investigate the epidemiology and genetic diversity of these coronaviruses,we conducted a longitudinal surveillanc...Previous studies indicated that fruit bats carry two betacoronaviruses,BatCoV HKU9 and BatCoV GCCDC1.To investigate the epidemiology and genetic diversity of these coronaviruses,we conducted a longitudinal surveillance in fruit bats in Yunnan province,China during 2009–2016.A total of 59(10.63%)bat samples were positive for the two betacorona-viruses,46(8.29%)for HKU9 and 13(2.34%)for GCCDC1,or closely related viruses.We identified a novel HKU9 strain,tentatively designated as BatCoV HKU9-2202,by sequencing the full-length genome.The BatCoV HKU9-2202 shared 83%nucleotide identity with other BatCoV HKU9 stains based on whole genome sequences.The most divergent region is in the spike protein,which only shares 68%amino acid identity with BatCoV HKU9.Quantitative PCR revealed that the intestine was the primary infection organ of BatCoV HKU9 and GCCDC1,but some HKU9 was also detected in the heart,kidney,and lung tissues of bats.This study highlights the importance of virus surveillance in natural reservoirs and emphasizes the need for preparedness against the potential spill-over of these viruses to local residents living near bat caves.展开更多
文摘The paper reviewed the function mechanism of Bilken virusicide against rice stipe disease, and then introduced its control effects in field test as well as its application method.
文摘[Objective] The efficacy of difenoconazole-azoxystrobin 32.5% SC for rice sheath blight and its application technology were discussed in this research. [Method] Three surveys were carried out. There were 5 fixed test clumps in each plot. The number of total plants and disease plants and disease progression in each fixed clump were recorded. The correlation effectiveness was calculated based on the growth rate of disease index. Significance analysis was performed with Dun- can＇s new multiple range method （DMRT）. [Result] The difenoconazole-azoxystrobin 32.5% SC had a good efficacy for rice sheath blight, and its efficacy increased with the increase of dose. If sprayed according to the dose of 450 ml/hm2 5 days before the beginning of heading stage of rice, difenoconazole-azoxystrobin 32.5% SC would have better effects on controlling rice sheath blight with correlation effectiveness reaching up to 95.14%, which was 2.06% higher than that of control drug （trifloxys- trobin-tebuconazole 75% WG, 225 g/hm2）. [Conclusion] Treated with difenoconazole- azoxystrobin 32.5% SC with dose of 450 ml/hm2, rice would have green upper leaves and less yellow middle and lower leaves. Moreover, the yield was in- creased significantly. The difenoconazole-azoxystrobin 32.5% SC had good safety. Therefore, difenoconazole-azoxystrobin 32.5% SC had a good application prospect in production.
基金supported by the China Natural Science Foundation (81290341 and 31621061 to ZLS)United States Agency for International Development Emerging Pandemic Threats PREDICT project (AID-OAA-A-14-00102)National Institute of Allergy and Infectious Diseases of the National Institutes of Health (Award Number R01AI110964)
文摘Previous studies indicated that fruit bats carry two betacoronaviruses,BatCoV HKU9 and BatCoV GCCDC1.To investigate the epidemiology and genetic diversity of these coronaviruses,we conducted a longitudinal surveillance in fruit bats in Yunnan province,China during 2009–2016.A total of 59(10.63%)bat samples were positive for the two betacorona-viruses,46(8.29%)for HKU9 and 13(2.34%)for GCCDC1,or closely related viruses.We identified a novel HKU9 strain,tentatively designated as BatCoV HKU9-2202,by sequencing the full-length genome.The BatCoV HKU9-2202 shared 83%nucleotide identity with other BatCoV HKU9 stains based on whole genome sequences.The most divergent region is in the spike protein,which only shares 68%amino acid identity with BatCoV HKU9.Quantitative PCR revealed that the intestine was the primary infection organ of BatCoV HKU9 and GCCDC1,but some HKU9 was also detected in the heart,kidney,and lung tissues of bats.This study highlights the importance of virus surveillance in natural reservoirs and emphasizes the need for preparedness against the potential spill-over of these viruses to local residents living near bat caves.
