黑龙江省“水玉米”产生的原因及解决途径

本文客观地分析了黑龙江省“水玉米”产生的原因 ,对种植不同熟期玉米品种的经济效益进行了比较。提出了黑龙江省“水玉米”的解决途径 。

Effects of Controlled Release Fertilizer on Loss of Nitrogen and Phosphorus from Farmland

[Objective] The aim was to study on effects of controlled release fertilizer on loss of nitrogen and phosphorus from farmland. [Method] Experiment was conducted in fields planted with rice and corn around Chao Lake and effects of compound fertilizer, controlled release fertilizer and controlled release fertilizer （reduced by 20%） on loss of nitrogen and phosphorus through runoff and leaching were analyzed. [Result] Loss of nitrogen and phosphorus mainly occurred in early stage of fertilizing; loss caused by runoff accounted for over 98% and caused by leaching was lower than 2%, indicating that nutrients of rice and corn mainly lost through runoff. As for controlled release fertilizers with 20% reduced, total loss of N and P decreased by 60% and 63% in rice field and reduced by 27.8% and 34% in corn field, respectively, indicating that controlled release fertilizer would maintain nutrients in soils high in later period of plant growth, improve use efficiency of N and P, reduce N and P loss in rice and corn fields in rainy season, and decrease non-point pollution. [Conclusion] The research suggested that controlled release fertilizer would slow down the loss of nutrients in farmlands, providing scientific references and technological support for extension of controlled release fertilizer and reduction of agricultural non-point pollution.

Dry Matter Accumulation,Moisture Content in Maize Kernel and Their Influences on Mechanical Harvesting

[Objective] The aim of this study was to investigate the dry matter accumulation,moisture content in maize kernel and their influences on mechanical harvesting.[Methods] Using Zhengdan 958,Xianyu 335,Hongda 8 and Liyu 16 as experimental materials,we preliminarily measured the dynamic changes of dry matter accumulation and moisture content with the days after pollination lasted,and investigated the effect of moisture content in kernels on mechanical harvesting of Zhengdan 958 and Liyu 16.[Results] During summer growing season,Zhengdan 958 and Liyu 16 became physiologically mature and suitable for harvesting at about 51 days after pollination,Xianyu 335 and Hongda 8 required 58 days to become physiologically mature and suitable for harvesting after pollination.At physiologically mature stage,the moisture contents in kernels of Zhengdan 958,Xianyu 335,Hongda 8 and Liyu 16 were respectively 33.74%,28.86%,32.05% and 35.24%,respectively.Adopting mechanical harvesting at physiologically mature stage,the kernel loss rate of Zhengdan 958 and Liyu 16 was consistent with the range of mechanical operation index（kernel loss rate of 0.84%≤2%）,while the percentage of broken kernels did not accord with the range of mechanical operation index（kernel crashing rate of 10.12%≧1%）.The results indicate that Zhengdan 958 and Liyu 16 are not suitable for mechanical harvesting using full feeding combine havester.[Conclusion] This study provided theoretical basis for improving the maize harvest index under delayed harvesting.

Effects of Super Absorbent Polymers on Yield and Water-saving and Drought-escaping Mechanism in Spring Maize

The effects of super absorbent polymers （SAP） on yield as well as water-saving and drought-escaping mechanism in spring maize in the seasonal drought region were studied. As shown by the results, during the seasonal drought in southern China, SAP treatment promoted the soil moisture, improved the capability of absorption and transportation of roots, promoted physiological and biochemical functions, increased the chlorophyll content, photosynthetic rate and intercellular CO2 concentration, and reduced the stomatal conductance and transplre.tion rate. As a result, the economic characters of spring maize were improved, and the yield was increased.

Isolation and Structural Analysis of DRE-Binding Transcription Factor from Maize (Zea mays L.)

Dehydration-responsive element-binding (DREB) proteins specifically binding with dehydration-responsive element (DRE) have been identified as a kind of important transcription activator of plants under drought, high salt and cold stress. The conserved amino, acid residues of Val (14th residue) and Glu (19th residue) in AP2/EREBP domain of DREB1A have been identified to be two key points in determining the binding ability of DREB gene with DRE element. Using the yeast one-hybrid system, we isolated one maize DREB gene named maDREB1 by screening cDNA library. Trans-activation experiment in yeast reporter strain demonstrated that maDREB1 protein could function as a DREB transcription factor activating target gene expression by specifically binding to the DRE cis-element. To assess the functional significance of these two residues in maDREB1, the V14 and E19 were substituted individually or doubly by Ala and Asp. Point mutation analysis showed that V14 substitution made significant loss of binding ability with DRE element, while point mutation of E19 had less effect. If the substitution happened simultaneously to these two residues, it would lead to great loss of the ability of binding with DRE element. It suggested that V14 and E19 were both important in protein-DNA interacting in maDREB1, though 14V was more essential. The copy number and expression pattern of maDREB1 was discussed.

The Degradation of FB1 in Aqueous Acetonitrile and Corn Following γ-ray Irradiation

To investigate the degradation of FB1 in aqueous acetonitrile and corn af-ter γ-ray irradiation, the radiolytic products of FB1 was detected preliminarily. The results showed that γ-ray irradiation could degrade FB1 in aqueous acetonitrile;When the radiation dose was below 9 kGy, the degradation of FB1 in corn was not significant. The degradation rates of FB1 with concentrations of 0.8 mg/ml, 10.0 μg/ml, 1.0 μg/ml and 50 ng/ml after irradiation at 9 kGy were 22.5%, 51.0%, 59.0% and 64.8% respectively; when irradiation dose was increased to 100 kGy, the degrada-tion rate of FB1 with concentration of 0.8 mg/ml was up to 90%, and it was nearly 100% when irradiation dose was increased to 200 kGy. No representative products of FB1 were detected by LC/MS/MS analysis.

Effects of Dilute Acid-intensified Hydrolysis on Fermentative Biohydrogen Production Capacity of Maize Stalk

[Objective] This study was to explore the effects of dilute acid hydrolysis on fermentative biohydrogen production capacity of maize stalk. [Method] Using maize stalks subjected to mechanical disintegration,steam explosion and dilute acid hydrolysis as experimental materials,we measured and analyzed the effects of different treatments and particle size of maize stalk were analyzed. [Result] The optimal fermentative biohydrogen production was found under following parameters：pretreatment of 0.8% dilute H2SO4 following steam explosion,particle size of maize stalk of 0.425-0.850 mm,liquid-solid ratio [0.8% H2SO4 （M）：stalk （W）] of 10：1. [Conclusion] Post steam explosion,dilute 0.8% dilute H2SO4 intensified hydrolysis on maize stalk could produce fermentative biohydrogen production capacity.

Effect of Fertilizing Level and Planting Densities on Yield and Nitrogen Utilization in Maize

Elite maize hybrid Guidan0810 was selected as material, and the effects of fertilizing level and planting densities on yield and nitrogen utilization were dis- cussed in the study. In field experiments as per double-cropping system, 4 main plots （fertilization levels） and 6 subplots （planting densities） were set in a split plot design. The results suggested that yield had close relationship with fertilization levels and planting densities. Different fertilization levels and planting densities significantly affected yield. With the increase of nitrogen fertilization, nitrogen use efficiency, nitrogen agronomic efficiency and nitrogen physiological efficiency declined. Under the same fertilization level, nitrogen use efficiency, nitrogen agronomic efficiency and nitrogen physiological efficiency grew a little with the increase of planting density, so nitrogen efficiency could be improved by regulating planting density. The results also showed that A2 （including N 225.0 kg/hm2, P205 75.0 kg/hm^2, K20 187.5 kg/hm^2） matching to B3 （52 500 plants/hm^2） or B4（60 000 plants/hm^2） was a better design, which could obtain a higher yield in the range of 7 913.2-8 207.8 kg/hm2, respectively.

Doubling Efficiency of Maize Haploids Treated with Different Methods

Haploid seedlings were inducted from different maize materials.At 2-3-leaf stage,maize haploids were treated with 0.06% colchicine and 2.0% dimethyl sulfoxide（DMSO） by dipping root,dripping heart leaf and acupuncturing growing point,respectively.The doubling rate and mortality rate in different treatments were analyzed by variance analysis and multiple comparisons.The result showed that growing point acupuncturing method exhibited the highest doubling efficiency with an average doubling rate of 23%,seed-setting rate of 21.4%,and mortality rate of 16.3%.Composed with other two chemical doubling methods,growing point acupuncturing method significantly improved the doubling rate of maize haploids with a lower application dose of colchicine.This study laid the foundation for industrial application of haploid breeding techniques.

Optimization of Hydrolysis Conditions for Corn Gluten Meal

[Objective] To investigate the optimal enzyme for the hydrolysis of corn gluten meal and the optimal hydrolysis conditions for the enzyme. [Method] Nine kinds of enzymes were used to hydrolyze the corn gluten meal, using the formaldehyde titration method for the determination of hydrolysis degree, and orthogonal test was used to determine the optimal hydrolysis conditions for double enzymes hydrol- ysis of corn gluten meal. [Result] The optimal pretreatment condition for corn gluten meal is heating at 121 ~C for 30 min. The double enzyme hydrolysis for the pro- treated corn gluten meal using 2709 alkaline protease and flavourzyme showed that the degree of hydrolysis could reach 32.4% with enzyme addition amount of 4%, hy- drolysis time of 4 h at 45℃ and pH=7.0. [Conclusion] This study laid the foundation for the study on the preparation of bioactive peptides such as oligopeptide with high F value and antihypertensive peptides, further improving the corn intensive process- ing industrial chain.

Influential Factors of Water Productivity of Maize in Oasis of Arid Areas——A Case Study of Linze County

[Objective] The aim was to research influential factors of water productivity of maize in oasis in arid areas. [Method] In middle reach of Heihe River in Linze County of Zhangye City, maize, a major crop in irrigated area in the oasis, was studied and nine influential factors were chosen through grey relation analysis. [Result] According to grey relation analysis, top five factors influencing water produc- tivity of maize during 1995-2009 were as follows： applied quantity of chemical fertil- izers〉labour input〉seeds〉mulch amount〉pesticide amount, which indicated that controllable factors, instead of uncontrollable ones, dominate in influence of water productivity of maize in oasis in arid areas. [Conclusion] With water resource limited, investment of controllable factors in high-efficient agricultural production should be the major strategy to improve water productivity of maize in oasis of arid areas.

Effect of Nitrogen Management on Yield and Water Use Efficiency of Rainfed Wheat and Maize in Northwest China

A field experiment with four treatments and four replicates in a randomized complete block design was conducted at the Changwu Experimental Station in Changwu County, Shaanxi Province, of Northwest China from 1998 to 2002. The local cropping sequence of wheat, wheat-beans, maize, and wheat over the 4-year period was adopted. A micro-plot study using ^15N-lahelled fertilizer was carried out to determine the fate of applied N fertilizer in the first year. When N fertilizer was applied wheat （years 1, 2 and 4） and maize （year 3） grain yield increased significantly （P 〈 0.05） （〉 30%）, with no significant yield differences in normal rainfall years （Years 1, 2 and 3） for N application at the commonly application rate and at 2/3 of this rate. Grain yield of wheat varied greatly between years, mainly due to variation in annual rainfall. Results of ^15N studies on wheat showed that plants recovered 36.6%-38.4% of the N applied, the N remained in soll （0-40 cm） ranged from 29.2% to 33.6%, and unaccounted-for N was 29.5%-34.2%. The following crop （wheat） recovered 2.1%- 2.8% of the residual N from N applied to the previous wheat crop with recovery generally decreasing in the subsequent three crops （beans, maize and wheat）.

Groundwater Contamination with NO_3-N in a Wheat-Corn Cropping System in the North China Plain

The North China Plain,where summer corn(Zea mays L.)and winter wheat(Triticum aestivum L.)are the major crops grown,is a major agricultural area in China.Permeable soils make the region susceptible to groundwater pollution by NO_3-N,which is applied to fields in large amounts of more than 400 kg NO_3-N ha^(-1)as fertilizer.A field experiment was established in 2002 to examine the relationship among N fertilization rate,soil NO_3-N,and NO_3-N groundwater contamination.Two adjacent fields were fertilized with local farmers' N fertilization rate(LN)and double the normal application rate(HN),respectively,and managed under otherwise identical conditions.The fields were under a traditional summer corn/winter wheat rotation.Over a 22-month period,we monitored NO_3-N concentrations in both bulk soil and soil pore water in 20-40 cm increments up to 180 cm depth.We also monitored NO_3-N concentrations in groundwater and the depth of the groundwater table.No significant differences in soil NO_3-N were observed between the LN and HN treatment.We identified NO_3-N plumes moving downward through the soil profile.The HN treatment resulted in significantly higher groundwater NO_3-N,relative to the LN treatment,with groundwater NO_3-N consistently exceeding the maximum safe level of 10 mg L^(-1),but groundwater NO_3-N above the maximum safe level was also observed in the LN treatment after heavy rain.Heavy rain in June,July,and August 2003 caused increased NO_3-N leaching through the soil and elevated NO_3-N concentrations in the groundwater.Concurrent rise of the groundwater table into NO_3-N- rich soil layers also contributed to the increased NO_3-N concentrations in the groundwater.Our results indicate that under conditions of average rainfall,soil NO_3-N was accumulated in the soil profile.The subsequent significantly higher- than-average rainfalls continuously flushed the soil NO_3-N into deeper layers and raised the groundwater table,which caused continuous groundwater contamination with NO_3-N.The results suggest that under common farming practices in the North China Plain,groundwater contamination with NO_3-N was likely,especially during heavy rainfalls,and the degree of groundwater contamination appeared to be proportional to the N application rates.Decreasing fertilization rates, splitting fertilizer inputs,and optimizing irrigation scheduling had potential to reduce groundwater NO_3-N contamination.

Coupled Effects of Soil Water and Nutrients on Growth and Yields of Maize Plants in a Semi-Arid Region

Interaction between soil water and nutrients plays an important role in sustainable crop management in semi-arid environments.On the basis of a field experiment conducted from 2000 to 2003,this study examined the coupled effects of irrigation and fertilizers on maize growth and yield in a semi-arid region of northeastern China.In terms of plant productivity,nitrogen fertilizer had the most significant effect followed by irrigation and phosphate levels.The combined application of nutrients and irrigation exerted a synergistic effect on the grain yield of maize plants.Regression analysis indicated that optimal levels of nitrogen and phosphate,in addition to adequate irrigation,could greatly improve the efficiency of grain production.Similarly,optimization of soil nutrient availability substantially increased water use efficiency.These suggested that for the most efficient and sustainable crop production,irrigation and nutrient management should be based on a quantitative understanding of water/nutrients interaction,particularly in semi-arid and arid regions.

Effect of Limited Single Irrigation on Yield of Winter Wheat and Spring Maize Relay Intercropping

A field experiment was conducted during the 2002/2003 cropping season of winter wheat （Triticum aestivum） and spring maize （Zea mays） to evaluate the effect of limited single drip irrigation on the yield and water use of both crops under relay intercropping in a semi-arid area of northwestern China. A controlled 35 mm single irrigation, either early or late, was applied to each crop at a certain growth stage. Soil water, leaf area, final grain yield and yield components such as the thousand-grain weight, length of spike, fertile spikelet number, number of grains per spike, and grain weight per spike were measured, and water use efficiency and leaf area index were calculated for the irrigated and non-irrigated relay intercropping treatments and sole cropping controls. The results showed that yield, yield components, water use efficiency, and leaf area index in the relay intercropping treatments were affected by limited single drip irrigation during various growth stages of wheat and maize. The total yields in the relay intercropping treatment irrigated during the heading stage of wheat and the heading and anthesis stage of maize were the highest among all the treatments, followed by that irrigated during the anthesis stage of wheat and silking stage of maize; so was the water use efficiency. Significant differences occurred in most yield components between the irrigated and non-irrigated relay-intercropping treatments. The dynamics of the leaf area index in the relay-intercropped or solely cropped wheat and maize showed a type of single-peak pattern, whereas that of the relay intercropping treatments showed a type of double-peak pattern. Appropriately, limited single irrigation and controlled soil water content level could result in higher total yield, water use efficiency, and leaf area index, and improved yield components in relay intercropping. This practice saved the amount of water used for irrigation and also increased the yield. Therefore, heading stage of wheat and heading and anthesis stage of maize were suggested to be the optimum limited single irrigation time for relay-intercropped wheat and maize in the semi-arid area.

Water Requirements and Irrigation Scheduling of Spring Maize Using GIS and CropWat Model in Beijing-Tianjin-Hebei Region

Due to the over use of available water resources, it has become very important to define appropriate strategies for planning and management of irrigated farmland. In this paper, Beijing-Tianjin-Hebei (Jing-Jin-Ji) region was chosen as the case study area for its special political and economic status and its severe water problem. To achieve effective planning, the information about crop water requirements, irrigation withdrawals, soil types and climatic conditions were obtained in the study area. In the meantime, a GIS method was adopted, which extends the capabilities of the crop models to a regional level. The main objectives of the study are: 1) to estimate the spatial distribution of the evapotranspiration of spring maize; 2) to estimate climatic water deficit; 3) to estimate the yield reduction of spring maize under different rainfed and irrigated conditions. Based on the water deficit analysis, recommended supplemental irrigation schedule was developed using CropWat model. Compared to the rainfed control, the two or three times of supplemental water irrigated to spring maize at the right time reduced the loss of yield, under different scenarios.

Control Efficacy of Pyroxasulfone on Weeds in Corn Field

To explore the control efficacy of 85%pyroxasulfone water-dispersible granule(WG)on annual weeds in corn field as well as its safety,a randomized block design was used to conduct a field plot test of efficacy at four different test sites.After sowing before seedling,85%pyroxasulfone WG at the doses of 10,15,20,and 40 g/667 m^(2),and 55%acetochlor+atrazine suspension concentrate(SC,control agent)at the dose of 170 g/667 m^(2)in summer corn area and 260 g/667 m^(2)in spring corn area were applied,respectively.The test site without herbicide application was used as the blank control.The results revealed that after 40 d of application,the control efficacy of 85%pyroxasulfone WG reached 83.75%~98.23%on Gramineae weeds,55.22%~98.33%on broadleaf weeds,and 88.03%~98.46%on Cyperaceae weeds,but without toxicity to corns.Compared with 55%acetochlor+atrazine SC,85%pyroxasulfone WG at the dose of more than 10 g/667 m^(2)had higher control efficacy on Gramineae weeds,lower efficacy on broadleaf weeds and similar efficacy on Cyperaceae weeds.To sum up,spraying 85%pyroxasulfone WG at the dose of 15~20 g/667 m^(2)(an active component dose of 191.3~255.0 g/hm^(2))after sowing before seedling could effectively control some annual weeds in corn fields and had no toxicity to corns.The herbicide dose was greatly reduced,and thus 85%pyroxasulfone WG was recommended as an effective mean to control the main weeds in corn fields.

Effect of different mulch materials on winter wheat production in desalinized soil in Heilonggang region of North China

Freshwater shortage is the main problem in Heilonggang lower-lying plain, while a considerable amount of underground saline water is available. We wanted to find an effective way to use the brackish water in winter wheat production. Surface mulch has significant effect in reducing evaporation and decreasing soil salinity level. This research was aimed at comparing the effect of different mulch materials on winter wheat production. The experiment was conducted during 2002-2003 and 2003-2004. Four treatments were setup： （1） no mulch, （2） mulch with plastic film, （3） mulch with corn straw, （4） mulch with concrete slab between the rows. The result indicated that concrete mulch and straw mulch was effective in conserving soil water compared to plastic film mulch which increased soil temperature. Concrete mulch decreases surface soil salinity better in comparison to other mulches used. Straw mulch conserved more soil water but decreased wheat grain yield probably due to low temperature. Concrete mulch had similar effect with plastic film mulch on promoting winter wheat development and growth.

Downward Movement and Leaching of NO_3^--N fromNitrogen-Fertilized Corn on Dryland Soil

Field lysimeter method was employed to investigate the downward movement and leaching of N applied to summer corn (Zea may L.) on dryland soil in Beiling. A N-fertilizer (120 kg N hm-2) and a control treatment were arranged for the study. Soil solution was collected at depths of 20, 40, 60, 120 and 170 cm,while leachate was collected at the bottom (200 cm) of the lysimeter. The results showed that the downward movement of NO3-N in soil profile was greatly affected by rainfall pattern. The peak of leached NO3-N from both treatments coincided with the peak of the rainfall. In addition, leached NO3-N from both treatments and rainfall were significantly correlated (P<0.05). The amount of leached NO3-N was not great in the N-fertilizer treatment. The results also suggested that N fertilization could cause NO3-N contamination of groundwater during the rainy season.

Multi-objective regulation in autohydrolysis process of corn stover by liquid hot water pretreatment

Increasing reducing sugars(xylose and glucose) yield for bioethanol from corn stover depends strongly on optimization of pretreatment conditions. The optimum reaction conditions of two-stage liquid hot water(LHW) pretreatment based on total sugars yield were investigated. Under optimal conditions, the recovery of glucose of corn stover after two-stage LHW pretreatment and 72 h enzymatic digestion, reached 89.55%. In addition, acetic acid-rich spent liquor pretreatment and one-stage LHW pretreatment have been carried out to make comparisons with two-stage LHW treatment. Glucose yield 89.55% is superior to the recovery 83.38% using acetic acidrich spent liquor pretreatment or 80.58% using one-stage LHW pretreatment. The production of total sugars was increased by 7.8% when compared with one-stage pretreatment. Moreover, the structural features of the treated corn stover solid residues were also investigated by XRD and SEM technology in order to clarify the effects of the reaction on corn stover. The results indicated that the two-stage LHW pretreatment was an effective pretreatment method of corn stover to get most massive resource utilization, and it could be successfully applied to corn stover.