A high-performance liquid chromatography (HPLC) method has been developed for the first time to simultaneously quantify the four active ingredients, namely aloha, baicalein, aloe-emodin and wogonin, in Dangguilonghu...A high-performance liquid chromatography (HPLC) method has been developed for the first time to simultaneously quantify the four active ingredients, namely aloha, baicalein, aloe-emodin and wogonin, in Dangguilonghui tablet. The marker compotmds were separated on the Diamonsil C18 column at a flow rate of 1.0 mL/min with a mobile phase of methanol-acetonitrile-0.3% aqueous phosphoric acid (220: 4: 200, v/v/v) and while the detection wavelength was set at 225 nm. The assay was linear over the range of 1.450 - 29.00 ug/mL (r = 0.9992) for aloin, 0.4050 - 8.100ug/mL (r = 0.9994) for baicalein, 0.1100 - 2.200 ug/mL (r = 0.9997) for aloe-emodin, 0.2160 - 4.320 ug/mL (r = 0.9991) for wogonin, respectively. The average sample recoveries at three concentration levels were 100.7% (RSD = 0.88%), 101.0% (RSD = 0.89%), 100.0% (RSD = 1.3%) and 100.1% (RSD = 1.1%) for these constituents, respectively. This method is suitable for the quality control of Dangguilonghui tablet.展开更多
AIM:To investigate the associations between interleukin(IL)-1B and IL-1RN polymorphisms and gastric cancers among the Tibet,Hui and Han ethnicities.METHODS:Genomic DNA was extracted from peripheral blood of 210,205,an...AIM:To investigate the associations between interleukin(IL)-1B and IL-1RN polymorphisms and gastric cancers among the Tibet,Hui and Han ethnicities.METHODS:Genomic DNA was extracted from peripheral blood of 210,205,and 202 healthy volunteers and from 155,158,and 197 gastric cancer patients from the Tibet,Hui,and Han populations,respectively.Polymorphisms in IL-1B and IL-1RN were analyzed by denaturing high-performance liquid chromatography.RESULTS:Carriers of the IL-1B-31 CC genotype had an increased risk of intestinal type gastric cancer [odds ratio(OR) = 2.17,P = 0.037] in the Tibet ethnicity.Carriers of the IL-1B 2/L genotype had an increased risk of both intestinal and diffuse types of gastric cancer(OR = 2.08,2.31,P = 0.007,0.016,respectively) in the Hui ethnicity.In the Han population,carriers of the IL-1B-31 CC,IL-1B-511CT,TT genotypes had increased risk of intestinal type gastric cancer(OR = 2.51,2.74,5.66,P = 0.005,0.002,0.000,respectively).CONCLUSION:IL-1B and IL-RN genotypes may differentially contribute to gastric cancer among the Tibet,Hui,and Han ethnicities in the Qinghai area of China.展开更多
0.2000g mogroside sample is digested by 7.0ml nitric acid and 3.0ml hydrogen peroxide, and generated by coprecipitation enrichment hydride - atomic fluorescence spectrometry determines trace selenium in it. Under opti...0.2000g mogroside sample is digested by 7.0ml nitric acid and 3.0ml hydrogen peroxide, and generated by coprecipitation enrichment hydride - atomic fluorescence spectrometry determines trace selenium in it. Under optimum experiment conditions, a standard curve was produced using selenium standard solution, the linear equation is Ise=2890.6c+0.0012,compared wish the linear relaticnship is not by coprecipitation direct determination of standard series Ise=82.56c+0.0018 Selenium detection sensitivity was increased by 35.01 times,the correlation coefficient of obtained linear regression equation is 0.9996, the detection limit is 0.0012~tg/L and the relative standard deviation is 0.76% (n=5). Containing 0.1808pg.g-1 Se Luo Han Shen samples. Based on mogroside sample, add a certain of standard solution to do recovery experiment, the obtained recovery rate is in 95.8%-103.6% and the result is satisfactory.展开更多
The present paper reviews new findings in redoxproperties of the active constituent of Chinese herbalmedicine(CHM),a kind of CHM or a compoundprescription as an antioxidant.Firstly,we have studiedtheir antioxidant and...The present paper reviews new findings in redoxproperties of the active constituent of Chinese herbalmedicine(CHM),a kind of CHM or a compoundprescription as an antioxidant.Firstly,we have studiedtheir antioxidant and prooxidant actions with electronspin resonance(ESR).The results show that the activecomponents from over 10 kinds of CHM are able toscavenge the oxygen free radicals but propyl gallate展开更多
Objective To reveal the distribution characteristics of serum lipids,lipoproteins,and apolipoproteins levels in different nationalities.Methods Quantitative levels of those traits mentioned above were determined and b...Objective To reveal the distribution characteristics of serum lipids,lipoproteins,and apolipoproteins levels in different nationalities.Methods Quantitative levels of those traits mentioned above were determined and body height(H),weight(W)and body mass index(BMI=W/H2)were assessed in 773 Kazaks(men 360 and women 413)and 911 Han nationality(men 466 and women 445)from the Xinjiang Autonomous Region of China.Results Kazaks men and women,respectively,had significantly higher serum levels of HDL-c and ApoA1 (all P < 0.001)and significantly lower serum levels of TG(men P < 0.01,women P < 0.001),Lp(a)(allP<0.005),ApoB(men P<0.005,women P<0.001)and ApoB/A1(men P<0.01,women P<0.001)than their Han nationality controls.The threshold points of ApoA1(< 1.2 g/L)and ApoB(> 1.2 g/L)are higher in Hans than in Kazaks for ApoA1(20.7 vs 9.8%)and ApoB(18.6 vs 14.3%);the serum levels of TG,HDL-c,Lp(a),ApoA1 and ApoB/A1 are all closely correlated with nationality(P < 0.001,P = 0.001,and P < 0.05,respectively).Conclusion The results suggest that the nationality differences exist in serum levels of lipids,lipoproteins,and apolipoproteins,and Kazaks have a superior serum lipid pedigree to Hans.This differences may come from genetic differences,which affect the serum levels of lipids,lipoproteins,and apolipoproteins by controlling lipid metabolism pattems.Future study will be needed to dissect to the role of genetic factors on serum lipids.展开更多
文摘A high-performance liquid chromatography (HPLC) method has been developed for the first time to simultaneously quantify the four active ingredients, namely aloha, baicalein, aloe-emodin and wogonin, in Dangguilonghui tablet. The marker compotmds were separated on the Diamonsil C18 column at a flow rate of 1.0 mL/min with a mobile phase of methanol-acetonitrile-0.3% aqueous phosphoric acid (220: 4: 200, v/v/v) and while the detection wavelength was set at 225 nm. The assay was linear over the range of 1.450 - 29.00 ug/mL (r = 0.9992) for aloin, 0.4050 - 8.100ug/mL (r = 0.9994) for baicalein, 0.1100 - 2.200 ug/mL (r = 0.9997) for aloe-emodin, 0.2160 - 4.320 ug/mL (r = 0.9991) for wogonin, respectively. The average sample recoveries at three concentration levels were 100.7% (RSD = 0.88%), 101.0% (RSD = 0.89%), 100.0% (RSD = 1.3%) and 100.1% (RSD = 1.1%) for these constituents, respectively. This method is suitable for the quality control of Dangguilonghui tablet.
基金Supported by Grants from the National Natural Science Foun dation of China,No.30860259the Youth Scientific Re search Foundation of Qinghai University,No.2008-QY-09
文摘AIM:To investigate the associations between interleukin(IL)-1B and IL-1RN polymorphisms and gastric cancers among the Tibet,Hui and Han ethnicities.METHODS:Genomic DNA was extracted from peripheral blood of 210,205,and 202 healthy volunteers and from 155,158,and 197 gastric cancer patients from the Tibet,Hui,and Han populations,respectively.Polymorphisms in IL-1B and IL-1RN were analyzed by denaturing high-performance liquid chromatography.RESULTS:Carriers of the IL-1B-31 CC genotype had an increased risk of intestinal type gastric cancer [odds ratio(OR) = 2.17,P = 0.037] in the Tibet ethnicity.Carriers of the IL-1B 2/L genotype had an increased risk of both intestinal and diffuse types of gastric cancer(OR = 2.08,2.31,P = 0.007,0.016,respectively) in the Hui ethnicity.In the Han population,carriers of the IL-1B-31 CC,IL-1B-511CT,TT genotypes had increased risk of intestinal type gastric cancer(OR = 2.51,2.74,5.66,P = 0.005,0.002,0.000,respectively).CONCLUSION:IL-1B and IL-RN genotypes may differentially contribute to gastric cancer among the Tibet,Hui,and Han ethnicities in the Qinghai area of China.
文摘0.2000g mogroside sample is digested by 7.0ml nitric acid and 3.0ml hydrogen peroxide, and generated by coprecipitation enrichment hydride - atomic fluorescence spectrometry determines trace selenium in it. Under optimum experiment conditions, a standard curve was produced using selenium standard solution, the linear equation is Ise=2890.6c+0.0012,compared wish the linear relaticnship is not by coprecipitation direct determination of standard series Ise=82.56c+0.0018 Selenium detection sensitivity was increased by 35.01 times,the correlation coefficient of obtained linear regression equation is 0.9996, the detection limit is 0.0012~tg/L and the relative standard deviation is 0.76% (n=5). Containing 0.1808pg.g-1 Se Luo Han Shen samples. Based on mogroside sample, add a certain of standard solution to do recovery experiment, the obtained recovery rate is in 95.8%-103.6% and the result is satisfactory.
文摘The present paper reviews new findings in redoxproperties of the active constituent of Chinese herbalmedicine(CHM),a kind of CHM or a compoundprescription as an antioxidant.Firstly,we have studiedtheir antioxidant and prooxidant actions with electronspin resonance(ESR).The results show that the activecomponents from over 10 kinds of CHM are able toscavenge the oxygen free radicals but propyl gallate
文摘Objective To reveal the distribution characteristics of serum lipids,lipoproteins,and apolipoproteins levels in different nationalities.Methods Quantitative levels of those traits mentioned above were determined and body height(H),weight(W)and body mass index(BMI=W/H2)were assessed in 773 Kazaks(men 360 and women 413)and 911 Han nationality(men 466 and women 445)from the Xinjiang Autonomous Region of China.Results Kazaks men and women,respectively,had significantly higher serum levels of HDL-c and ApoA1 (all P < 0.001)and significantly lower serum levels of TG(men P < 0.01,women P < 0.001),Lp(a)(allP<0.005),ApoB(men P<0.005,women P<0.001)and ApoB/A1(men P<0.01,women P<0.001)than their Han nationality controls.The threshold points of ApoA1(< 1.2 g/L)and ApoB(> 1.2 g/L)are higher in Hans than in Kazaks for ApoA1(20.7 vs 9.8%)and ApoB(18.6 vs 14.3%);the serum levels of TG,HDL-c,Lp(a),ApoA1 and ApoB/A1 are all closely correlated with nationality(P < 0.001,P = 0.001,and P < 0.05,respectively).Conclusion The results suggest that the nationality differences exist in serum levels of lipids,lipoproteins,and apolipoproteins,and Kazaks have a superior serum lipid pedigree to Hans.This differences may come from genetic differences,which affect the serum levels of lipids,lipoproteins,and apolipoproteins by controlling lipid metabolism pattems.Future study will be needed to dissect to the role of genetic factors on serum lipids.