晓苏小说的生命伦理叙事——以“油菜坡”系列小说为例

晓苏“油菜坡”系列小说表现出多样的生命叙事形态,他擅长表现生命个体的自在生命状态、自为生存方式和自由生命体验。作家塑造了独特的生命群体:寻求理解和爱的光棍儿群体、求爱而不得的压抑中年女性和激情尚存的农村老年人。通过探究,晓苏热衷关注自由生命伦理叙事的原因在于他对人的生命主体与生命意识的尊重、对理想生命形态的追求与浪漫诗意的生命关怀、对自我生命苦难的抗争与对他人痛苦生命的悲悯。

“油菜联合收割机研制与开发”项目通过鉴定验收

2007年11月4日，江苏省科技厅、农机局在镇江市组织对江苏沃得农业机械有限公司、江苏大学、镇江市农业机械技术推广站和江苏省农业机械技术推广站联合承担的“油菜联合收割机研制与开发”项目进行验收，同时对新研制的“4LYB1—2．0型履带式油菜联合收割机”进行了科技成果鉴定。

Different Foliar Fertilizer Treatments Affect the Yield and Quality of Brassica napus L.for Both Vegetable and Oil Dual-Use Rape

Field experiments were carried out to study the effect of different foliar fertilizer treatments on the agronomic traits,yield,and quality of Brassica napus L.for both vegetable and oil dual-use rape.The seedlings of‘Youtai 929’,‘Jinyoutai 1’,and‘Zhongshuang 11’were sprayed with potassium dihydrogen phosphate,naphthylacetic acid,and selenium fertilizer,and the agronomic traits,yield,and quality were determined.The results showed that the naphthylacetic acid treatment had the highest indexes before winter,followed by the selenium fertilizer treatment.At the harvesting stage,the plant height,number of siliquae,yield per plant,and number of effective branches on the main stem of the three varieties were the highest in the naphthylacetic acid treatment,followed by those in the potassium dihydrogen phosphate treatment.The fresh shoot quality was the best in the selenium fertilizer treatment and the second in the naphthylacetic acid treatment.‘Jinyoutai 1’applied with naphthylacetic acid had the highest economic benefit of 4971.78 CNY/667m^(2),which was 642.32 CNY/667m^(2) higher than that of the control.The findings suggested that naphthylacetic acid was suitable to be used as the foliar fertilizer for the seedlings of B.napus varieties for both vegetable and oilseed,and the additional application of selenium fertilizer demonstrated better effect.

Development of a KASP Marker on Chromosome A05 for Seed Oil Content of Brassica napus

In order to identify the molecular markers that can be widely used in the breeding of Brassica napus L.varieties with high seed oil content under different genetic backgrounds,we developed a Kompetitive Allele Specific PCR(KASP)marker for seed oil content on the basis of the results from available studies.The verification in the F_(2) population showed that the marker was closely linked to the quantitative trait locus(QTL)for oil content on chromosome A05.The findings helped to breed the‘Fengyou’varieties with high seed oil content in the middle reaches of the Yangtze River.

Effect of Foliar Fertilizer Spraying at Seedling Stage on the Growth Physiological Characteristics and Yield of Brassica napus L.

In order to select a suitable foliar fertilizer for Brassica napus L.at the seedling stage,using‘Fengyou 958’as the material,different foliar fertilizers including BR,Se,Si,BR+Si,BR+Se,Se+Si and BR+Se+Si were sprayed at the seedling stage to study their effect on the physiological characteristics,growth and yield of Brassica napus L..The results showed that the growth,chlorophyll content,soluble sugar content,soluble protein content,and yield of different treatments at the budding stage improved compared with the control.The effect of the Si+Se treatment was the best,followed by the Se and Si treatments.The chlorophyll content of the flower decreased continuously during the whole flowering period,and the chlorophyll content of the mature silique peel was higher than that of the seed.The content of soluble sugar in flowers was the highest in the early flowering stage,and the content of soluble sugar in leaves at the flowering stage was higher than that at the budding stage.The soluble sugar content in the harvested silique peel decreased gradually with the increase of time,and that in the silique peel and seed was similar at 35 d after pollination.The soluble protein content in the silique peel and seed decreased gradually at the mature stage,and the soluble protein content in the silique peel was higher than that in the seed at the same stage.Si+Se foliar fertilizer spraying at the seedling stage can promote the growth and yield of Brassica napus L.and can be applied in Brassica napus L.production.

Cloning of BjNAC102 Promoter and Construction of Expression Vector in Brassica juncea

Transcription factor NAC102 plays an important role in the abiotic stress responses of plants.In this study,the promoter sequence of 3000 bp located in the upstream of the BjNAC102 gene was cloned from Brassica juncea‘Sichuan Yellow Seed’by using the homologous cloning method.The expression vector of the GUS gene driven by the BjNAC102 promoter was constructed by seamless cloning technology.The results showed that the sequence of the promoter of the BjNAC102 gene contained many cis-acting elements involved in light responsiveness,gibberellinresponsive element,and auxin-responsive element.It was speculated that BjNAC102 played an important role in the abiotic stress response in Brassica juncea.The expression vector of the promoter of the BjNAC102 gene was constructed,which layed a foundation for further studies of the expression pattern of the BjNAC102 gene in Brassica juncea.

Growing Development and Physiological Characteristics of Seeds Harvested Different Days after Pollination and Their 7-day Seedlings of Two Yellow-Seeded Cultivars of Brassica napus L.

This study aimed to understand the growing development and physiological characteristics of seeds harvested different days after pollination and the seedlings emerged from the seeds of yellow-seeded Brassica napus L..Two yellow-seeded cultivars‘Hongyuan 558’and‘Qinyou 806’were selected in this study,and 50 seeds were collected 30,35,and 40 d after pollination,respectively.The seeds were weighed and placed on filter paper for hydroponic experiments.The seed germination rate was determined 3-5 d later.The physiological indicators including superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)activities and malondialdehyde(MDA)content were measured for the seeds harvested different days after pollination and the 7-day-seedlings emerged from the seeds.The results showed that the seed dry weights and germination rates of both‘Qinyou 806’and‘Hongyuan 558’increased as the days after pollination increased,especially 30-35 d after pollination.The germination rate and dry and fresh weights of the seeds showed no significant differences between the two cultivars,and almost all the seeds germinated 40 d after pollination.As the days after pollination increased,the SOD activities in the seeds of both cultivars first increased and then decreased,reaching their peaks 35 d after pollination,which may be related to encountering adverse stress.The POD activity in the seeds of‘Qinyou 806’kept declining,and that of‘Hongyuan 558’first increased and then decreased,which may be related to the antioxidant mechanism and adaptive regulation.The CAT activities in the seeds of both cultivars first decreased and then increased.The MDA content in the seeds of‘Qinyou 806’first decreased and then increased,while that of‘Hongyuan 558’kept reducing and was the highest in the seeds 30 d after pollination,which indicated‘Hongyuan 558’was mildly affected by the environment and had strong resistance.The activities of SOD and POD in the seedlings of two cultivars that were emerged from the seeds 35-40 d after pollination became weakened,which may be related to their exposure to stress.The CAT activity in the seedlings of‘Hongyuan 558’was generally higher than that of‘Qinyou 806’,indicating that‘Hongyuan 558’had stronger stress resistance.The MDA content in the seedlings emerged from the seeds 35-40 d after pollination showed a decreasing trend,indicating that the seedlings emerged from the seeds harvested at this stage had mild cell membrane damage and enhanced stress resistance.In conclusion,‘Hongyuan 558’had strong environmental adaptability and stress resistance.

Chloroplast Composition and Structural Differences in a Chlorophyll-reduced Mutant of Oilseed Rape Seedlings

对黄化突变体Cr352 9和野生型油菜 (BrassicanapusL .) 352 9叶绿体的超微结构和组成进行了比较。与野生型相比 ,突变体Cr352 9叶片具有较少的类囊体、较少的垛叠膜区和较少的叶绿素含量。突变体的Chla/Chlb比值较高 ,是野生型的 2倍。电泳结果表明 ,突变体类囊体膜中LHCⅡ和其三聚体LHCⅡ 的含量减少。SDS_PAGE分析显示 ,LHCⅡ的脱辅基蛋白在突变体类囊体膜中明显减少。免疫印迹进一步表明 ,所有LHCⅡ组分的含量仅为野生油菜的类囊体膜的 1 / 3。突变体Cr352 9的天线系统比野生型 352 9的小。

Transfer of Superior Traits from Brassica juncea into Brassica napus

Both Brassica juncea（mustard）and B.napus（rapeseed）are allotetraploid species of Brassica.Although these two species have their unique characters,they possess a common A genome and a few homeologous chromosomes between the genomes B and C,which provide the foundation for transfer of superior traits from B.juncea into worldwide cultivated B.napus.The unique characters of B.juncea were summarized,breeding procedure for introgression of superior traits from B.juncea into B.napus by the interspecific crossing proposed,and some recently developed rapeseed germplasm lines with novel traits such as yellow seed and super oil content,restorer of fertility for pol CMS,extreme early maturity,high oleic acid content and short-staure plant briefly introduced in this paper.

Phytogenetic Studies on Intergeneric Hybridization Between Brassica napus and Matthiola incana

The intergeneric hybridization between Brassica napus L. cv. oro and Matthiola incana (L.) R. Br. was carried out to study the phytogenetic peculiarities of the hybrid plants. In order to improve the oil quality of rape seed, ovaries of B. napus pollinated with pollen of M. incana, were cut off and inoculated onto MS media supplemented with various plant hormones at the 7th day after pollination. Two mature embryos were obtained from 750 pollinated ovaries cultured in vitro. The percentage of seed set was 0.26%. The mature embryos were transferred onto the MS media supplemented with 2.0 mg/L 6-BA + 0.1 mg/L NAA, and a compound bud was produced later. The compound bud was then cut into a number of single buds, which were transferred onto fresh media. Twenty-two plantlets in vitro were developed from the above single buds. The hybrid plants (F-1) were basically intermediate between the two parents in many,characters with a few showing hybrid vigor. The fertility of hybrid plants (F-1) was poor. Cytological studies revealed that the hybrid plants (F-1) were mixoploid. in nature. The chromosome number of many somatic cells was 2n = 26, which was the sum of the chromosome number of the two parents. The chromosome number of other somatic cells was 2n = 38, similar to that of B. napus. The hybrid offspring (F-2) from the selfed hybrid plants (F-1) showed polymorphism. Among the hybrid offspring (F-2) some were nearly matroclinous and fertile, similar to B. napus. some others were intermediate between the two parents and less fertile, and a few were poorly developed and nearly infertile. From the hybrid offspring a few plants with improved seed-oil quality were obtained.

Influential Factors of Restorer of New Cytoplasmic Male Sterile(NER) on Anther Culture

Some influential factors of anther culture were studied preliminarily by conducting anther culture of the restorers of new cytoplasmic male sterile （NER）. Several results were obtain from this experiment and they were listed as follow：① MS cultrure medium with such hormones as 2,4-D 2 mg/L,6-BA 0.5 mg/L, NAA 0.5 mg/L was the best suitable for callus induction of NER. ②The difference of induction rate was significantly different between different plant age groups. From the 110th day to 141th day,the induction rate was increased with the increase of age and the difference of induction rate reached 0.01 significant difference level. The induction rate reached the highest value in the 141th day then it declined gradually. ③The combined use of 2, 4-D and 6-BA with proper increase of 2,4-D was good for inducing callus. ④The green plantlet induction rate of NER was increased when the concentration of 6-BA increased from 2 mg/L to 4 mg/L. Adding ZT from 0.5 mg/L to 2 mg/L. 6-BA would led 2.47% increase of green plantlet olantlet induction rate.

Analysis of Yield and Disease Resistance Traits of New Winter Rapeseed Varieties over the Past Twenty Years in China

Based on the results of the national regional trail for winter rapeseed in four groups,including the upper reaches,middle reaches and lower reaches of Yangtze River and Huang-Huai region in the past twenty years,new varieties of winter rapeseed showed upward trend in average yield,the yield level in Huang-huai group was higher than other groups.The changes of average effective pods per plant were not significant in any group,but the number of grain per pod and 1 000-grain weight showed increase trend.However,the increment of grains per pod in three Yangtze River groups was higher than that in Huang-Huai River group,while the increment of 1 000-grain weight was just opposite.The incidence rate of Sclerotinia sclerotiorum and viral disease in new varieties of winter rapeseed obviously decreased,and the incidence rate of S.sclerotiorum decreased lower than 5%.Genetic improvement for winter rapeseed should be focus on the number of grain per pod and 1 000-grain weight in the future,and yield level of new varieties in all four groups is expected to increase.

Effects of Planting Density on Yield and Mechanical Harvesting Loss Rate of Brassica napus L.

[Objective] This study aimed to explore agronomical measures to reduce the mechanical harvesting loss of rapeseed. [Method] Two rapeseed cultivars, Ningza 19 and Ningza 21, with certain pod-cracking resistance, were employed in field experiments. The two-factor split plot design and randomized complete block design were adopted. The rapeseed seeds were directly sowed with four different seeding rates （1.50, 2.25, 3.00 and 3.75 kg/hm2）. A total of four treatments were designed （112 500, 225 000, 337 500 and 450 000 plants/hm2）. After ripe, the rapeseed was harvested with harvester. Then the yield and harvesting loss rate were determined. [Result] When the planting density ranged from 112 500 to 450 000 plants/hm2, the mechanical harvesting loss rate was decreased with the increase of planting density （Ningza 19, 7.54%-4.01%; Ningza 21, 7.19%-3.81%）. The total loss rates were all below 5% for the high plant densities, 337 500 and 450 000 plants/hm2. High planting density had certain regulating effects on plant type of rapeseed, including reducing plant height, reducing biomass per plant, reducing branch pod numbers per plant, weakening crossing and tangling among stems and improving ripening uniformity of pods. All the changes above were all conducive to reducing mechanical harvesting loss. In addition, the test results showed after the pods grew to maturity, especially when pods were yellow and the moisture content in grains was reduced to 11%, the mechanical harvesting loss only accounted for about 1% of the total field loss. In addition, the shattering loss, caused by mild col- lision, represented more than 90%, and the cleaning loss, occurred during the threshing and cleaning process, represented 4%-8% of the total field loss. The un- harvesting loss accounted for approximately 1% of the total loss. The shattering loss is closely related to cultivar characteristics, planting density, production level and other agronomic factors. The cleaning loss is determined by properties of harvesting machines. The unharvesting loss depends on mechanical properties ad skills of workers or farmers who drive harvesting machines. [Conclusion] In order to reduce mechanical harvesting loss, the rapeseed production should be improved from the perspectives of agricultural machinery and agronomic measures.

Distribution of Rapeseed Clubroot Disease in Hubei Province and Evaluation of Yield Loss

[Objective] The aim of this study was to reveal the distribution of rapeseed clubfoot disease in Hubei Province and to assess the yield loss caused by the pathogen, Plasmodiophora brassicae. [Method] Field surveys were conducted in Shayang, Dangyang, Zhijiang, Yidu and Changyang of Hubei Province during 2009- 2011. Clubfoot disease of rapeseed samples from the surveyed locations were con- firmed by PCR detection and plants infection experiment. The factors of yield and plot yields influenced by clubroot disease were determined in plot experiment. [Re- salt] Rapeseed clubroots were confirmed in Zhijiang and Dangyang and not found in Shayang and Yidu. Clubroot was found in cruciferous vegetables but not in rapeseed d in Changyang. Infection of P. brassicae significantly decreased of the first effective branch numbers of rapeseed, effective pod number per plant, seed number per pod and 1 000-grain weight. Yield of infected rapeseed decreased by 56.4% over non-infected control. [Conclusion] Rapeseed clubroot disease mainly distributed in Zhijiang and Dangyang of Hubei Province. The disease had an economic impact on rapeseed production.

Biomechanical Characteristics of Rape Stalks

[Objective] To study the correlation between the biomechanical properties of rape stalks and rape stem lodging. [Method] Through axial compression tests to the stalks of 4 different rape varieties, the change rules of maximum stem bearing ca- pacity, maximum compressive strength, elastic modulus and moment of inertia along plant height were analyzed, as well as the effect of different varieties and water contents on the biomechanical property indices of rape stalks. [Result] The maximum loads of rape stalks presented liner decrease trend along with the increase of stem height, and all reached the maximums below the height of 50 cm. The maximum stem compressive strength and elastic modulus of the 4 varieties were increased with ascending height, but in a slow rate with small change, thus the modulus of e- lasticity could be considered as unchanged. The maximum bearing capacity, maxi- mum compressive strength and elastic modulus of dry rape stalks were higher than wet stalks, indicating that the water contents of rape stalks had significant effect on their mechanical properties. According to the actual lodging situations in filed, stalks of variety No. 1 owned the worst biomechanical properties and lodging degree, while the biomechanical properties of No. 6 and F5 were better than No. 1 and No. 9, and they also had stronger lodging-resistance. [Conclusion] The study provides parameters and bases for the design of mechanized production and mechanical deep processing of crops, and can better reveal the physical natures of organisms. The methods used in this study can also be used to screen excellent crop stalks.

Effects of ABA on Photosynthetic Characteristics of Pods and Yield of Brassica napus

[Objective] The aim was to evaluate the effects of different concentrations of ABA on photosynthetic characteristics of pods and yield of Brassica napus and analyze the relationships between ABA concentration and yield components, to provide theoretical basis for cultivation of high-yield rapeseed. [Method] At the end of flowering stage, the rape plants were separately sprayed with 1, 2, 100 and 200μmol/L of ABA; photosynthetic parameters, water use efficiency （WUE）, yield and yield components of the rape plants were investigated 0, 5, 10 and 15 d later, respectively. [Result] ABA treatment with different concentrations significantly changed pod photosynthetic parameters and yield components of rapeseed. Low concentra- tions of ABA could improve pod＇s net photosynthetic rate and yield components while high concentrations had the contrary effect. [Conclusion] Whether ABA func- tioned in inhibiting or promoting role might depend on the interaction between endogenous hormone levels and exogenous ABA concentration. Improvement of WUE would be the main reason for yield increasing.

Screening of Drought-tolerant Brassica napus L.Varieties and Analysis on Their Physiologic and Biochemical Variations under Drought Stress

[Objective] The aim of this study was to screen drought-tolerant Brassica napus L. germplasm resources by analyzing their physiological and biochemical changes under drought stress. [Method] Forty varieties of B. napus varieties were cultured under PEG-6000 osmotic stress and extreme drought stress in pots, re- spectively. Then, the contents of chlorophyll, carotenoid, proline, malondialdehyde （MDA）, soluble sugar, soluble protein, and the activities of superoxide dismutase （SOD）, catalase（CAT）, peroxidase（POD） were measured under drought stress. [Result] Sever- al drought-tolerant varieties of B. napus were screened out： YAU200908, Xiangyou No.15, YAU200903, YAU200907, YAU200906 and YAU200904. Physiological and biochemical analysis showed that, the contents of chlorophyll and carotenoid de- creased with drought stress increasing; the contents of proline, soluble sugar, solu- ble protein, MDA and the activities of SOD,CAT, POD raised with drought stress in- creasing. [Conclusion] In the rapeseed varieties with stronger drought tolerance, the decrease in carotenoid content and the increase in proline content, soluble sugar content, MDA content, SOD activity, CAT activity were more obvious, so all these physiological and biochemical indices can be used to evaluate the drought tolerance of rapeseed.

Effect of Different Nitrogen Application Amount on the Net Increment of Fresh Dry Weight in Hybrid Rape

[ Objective] The aim of the research was to provide reference for reasonable application of nitrogen fertilizer for high yield.cultivation of hybrid rape cuhivar Youyan 9 and Youyan 10. [ Method] The net increment changes of individual plant fresh weight and dry matter weight of Youyan 9 and Youyan 10 with different nitrogen application treatments were studied. [ Result] The differences among average fresh weight increments of individual plant and average dry matter weight increment of individual plant with different treatments reached 0. 01 extremely significant level. Fresh weight increment and dry matter weight net increment of individual plant declined gradually with the increase of nitrogen application. In growtheourse ,fresh weight net increment of individual plant increased firstly then decreased and the maximum was in beginning flowering stage, besides that dry matter net increment increased gradually and the maximum was in mature period. The correlations among fresh net increment, dry matter weight net increment and yield net increment were positive or extremely positive. [ Conclusion] Under experimental condition, when nitrogen application was 225 kg/hm^2, hybrid rape Yanyou 9 and Yanyou 10 with low erucic,low glucosinolate could obtain high yield.

Effects of Brassinosteroid on Salinity Tolerance of Cotton

[Objective] In order to study the effects of brassinosteroid on salinity toler- ance of cotton. [Method] Three application modes （leaf application, root application and leaf/root application） of brassinosteroid for cotton under NaCI stress were de- signed to understand the effects of brassinosteroid on Na＋ and CI- accumulation, osmotic adjustment substance （proline） and biomass accumulation of cotton under NaCI stress. [Result] NaCI inhibited the growth of cotton seedlings. Compared with the control group, the biomass of cotton seedlings was decreased, Na＋ and CI- contents, MDA content and proline content were increased in cotton under NaCI stress. Under the NaCI stress, three application modes of brassinosteroid significant- ly improved the biomass, chlorophyll content in leaves, root activity and proline content; while Na~ and CI- content, MDA content were decreased. The enhancement of root activity and root physiological functions were more marked in root application and leaf/root application treatments than in leaf/root application treatment. [Conclu- sion] Three application modes of brassinosteroid all could alleviate the NaCI injuries on cotton, but root application and root/leaf application modes of brassinosteroid for cotton under NaCI stress are superior to the leaf application.

Cloning of Brassica napus EIN3 Gene and Its Expression Induced by Sclerotinia sclerotiorum

[Objective] The study was to investigate roles of Brassica napus EINB in （ BnEIN3 ） resistance to Sclerotinia sclerotiorum. [ Methods] Genomic PCR and RT-PCR were carded out to isolate genomic DNA and cDNA sequences of BnEIN3 from oilseed rape, based on the highly conserved region of EIN3 gene from Arabidopsis thaliana and the homologous sequences of oilseed rape ESTs. Expression levels of BnEIN3 were detected in three varieties of oilseed rape inoculated with S. sclerotiorum by real-time quantitative PCR.[Results] A 1 947 bp DNA fragment was obtained from oilseed rape. The fragment shared 82% identity to A. thaliana EIIV3, encoded 614 amino acids containing an EIN3 domain, and was named as BnEIN3. Real-time PCR results showed that expression patterns of BnEIN3 were drastically different in different varieties. In highly resistant oilseed rape variety D083, BnEIN3 expression level was significantly increased 72 h after S. sclerotiorum inoculation whereas in middle resistant and susceptible varieties Zhongshuang 9 and 84039, BnEIN3 expression was suppressed. [ Conclusion ] BnEIIV3 may play an important role in oilseed rape resistance to S. sclerotiorum.