凭“活体实验”获得博士学位的医师

池田苗夫曾是日本关东军第七三一部队(细菌部队)的一名军医,并在这支部队服役多年。战后,他利用在七三一部队进行人体实验的资料发表学术论文并获得了新大学的医学博士学位。文章介绍了池田苗夫参与七三一部队进行人体实验的活动的一些基本情况并呼吁日本医学界进行反省。

Impact of an active educational video game on children's motivation,science knowledge, and physical activity

Background： Active educational video games （AVGs） appear to have a positive effect on elementary school students＇ motivation leading to enhanced learning outcomes. The purpose of this study was to identify the effectiveness of an AVG on elementary school students＇ science knowledge learning, physical activity （PA） level, and interest-based motivation. Methods： In this randomized controlled study, 53 elementary school students were assigned to an experimental condition or a comparison condition. The experimental condition provided an AVG learning environment, whereas the comparison condition was based on sedentary educational video games. Results： The results of repeated measures analysis of variance （ANOVA） on the knowledge test showed that students in both groups performed better on the post-test than they did on the pre-test （p 〈 0.001, η2 = 0.486）, and their post-test scores did not differ significantly. The experimental condition provided a more active environment since the students＇ average heart rates （HRs） were in the Target-Heart-Rate-Zone （HR = 134 bpm）, which was significantly higher than the average HR （103 bpm） from the comparison condition （t = 7.212, p 〈 0.001）. Students in the experimental condition perceived a higher level of situational interest than their counterparts in the comparison group （p 〈 0.01, and η2 = 0.301）. Conclusion： These results suggest that AVGs benefit children more in terms of PA and motivation than traditional video games by providing an enjoyable learning experience and sufficient PA.

In Silico Screening of Potential Drug with Antileishmanial Activty and Validation of their Activity by in Vitro and in Vivo Studies

The research on discovery and development of new treatments for cutaneous leishmaniasis has been declared as priority. Using bioinformatics approaches, this study aimed to identify antileishmanial activity in drugs that are currently used as anti-inflammatory and wound healing by such anti-Leishmania activity was validated by in vitro and in vivo assays. In silico analysis identified 153 compounds from which 87 were selected by data mining of DrugBank database, 22 and 44 were detected by PASS （http：//pass.cribi.unipd.it） and BLAST （http：//blast.ncbi.nlm.nih. gov/） alignment, respectively. The majority of identified drugs are used as skin protector, anti-acne, anti-ulcerative （wound healer） or anti-inflammatory and few of them had specific antileishmanial activity. The efficacy as antileishmanial was validated in vitro in 12/23 tested compounds and in all seven compounds that were evaluated in in vivo assays. Notably, this is the first report of antileishmanial activity for adapalene. In conclusion, bioinformatics tools not only can help to reduce time and cost of the drug discovery process but also may increase the chance that candidates identified in silico which have a validated antileishmanial activity by combining different biological properties.

Respiratory syncytial virus infection in immunocompetent and immunocompromised murine

The purpose of this study is to distinguish respiratory syncytial virus （RSV） infection and immunology between immunocompetent and immunocompromised murine and to explore immune mechanism of RSV infection. At various time points after RSV infection of BALB/c mice and nude mice, pulmonary viral titers were assayed, RSV antigen was tested by direct immunofluorescent assay and immunohistochemistry. Pulmonary mRNA expressions of Toll like receptor （TLR）2 and TLR4 were assayed by RT-PCR. CD4^＋ ceils and CD8 ^＋ ceils in peripheral blood were examined by flow cytometry and plasma total IgE was assayed by ELISA. Leukocytes in bronchoalveolar lavage fluid （BALF） and pulmonary histology were identified to reflect airway inflammation. It was found that RSV titers of both mice peaked on the 3rd day post infection with a much higher level of viral titer in nude mice than in BALB/c mice and a longer viral duration in nude mice （over 9 days post infection） than in BALB/c mice （6 days post infection）. RSV infection induced higher viral antigen expression in nude mice （0.267 ±0.045） than in BALB/c mice （0. 168 ±0.031）. RSV infection enhanced pulmonary TLR4 expression of BALB/c mice （51.96% ±11.34%） and nude mice （48.96% ± 12.35%） compared with each control （34.04% ±10.06% and 32.37% ±9.87% respectively）. CD4^＋ peripheral blood ceils increased in RSV infected BALB/c mice （66.51% ±2.09% ） compared with the control BALB/c mice （51.63% ±5.90%）, and CD4^＋ ceils and CD8^＋ ceils were deficient in nude mice. RSV infection increased plasma total IgE in both mice, and BALB/c mice had a larger amount of IgE on the 7th day post infection （9.02 ng/ml ±2.90 ng/ml） and on the 14th day post infection （12.76 ng/ml ±4.15 ng/ml） than corresponding nude mice （3.72 ng/ml ±1.06 ng/ml and 7.62 ng/ml ±3.08 ng/ml respectively on the 7th and 14th day post infection）. RSV infected nude mice had more severe airway inflammation than infected BALB/c mice. It is concluded that BALB/c mice and nude mice presented similar RSV infectious characteristics. However, infection of nude mice showed higher viral titer with longer duration and more severe airway inflammation, lower level of plasm total IgE and CD4^＋ peripheral blood cells, but the similar pulmonary TLR4 expression with BALB/c mice.

An experimental research of fluid piston oscillation in square moon pool under the wave-flow combined conditions

The article studies the oscillation features of the water in the square moon pool under the circumstances of wave-flow combined conditions. Comparing with the results of experiments of the square moon pool, a series of studies reveal that the water piston oscillation phenomenon is quite similar to that of the circular one. Two types of oscillation are exhibited under different incoming wave periods, i.e. self-excited oscillation and forced oscillation. The difference between the circular and square moon pool is that the attack angle makes greater influences on the square one. "Beating phenomenon" is also found in the square moon pool which is not mentioned of the circular one.

Activation of Landslides in the South of Ukraine under the Action of Natural Seismic Impacts （Experimental and Analytical Studies）

Activation of seismic activity in the Vrancha area, in the Black Sea region resulted in considerable activation of landslides and in increasing of landslide hazard in earthquake-prone areas of Ukraine. Totally about 23,000 landslides were identified in the territory of Ukraine. Experimental and analytical studies of slumps in the Central Livadia landslide system were carried out with the aid of the ZSUV monitoring system. Experimental data were obtained concerning impact of natural seismic factors on the Central Livadia landslide system and on the Palace itself. The South-East wing of the Livadia Palace continuously vibrates relative to a certain midposition. The increase of the amplitude of the faqade deviation from the midposition may be caused by activation of slopes as a result of additional subsidence and ground water rise due to local earthquakes in the Black Sea and because of some other factors.

A versatile Pt-Ce6 nanoplatform as catalase nanozyme and NIR-Ⅱ photothermal agent for enhanced PDT/PTT tumor therapy

The hypoxic nature of solid tumors has severely negative effects on oxygen-based photodynamic therapy.In this study,we used porous Pt nanoparticles as a catalase(CAT)nanozyme,the second near-infrared(NIR-Ⅱ)region photothermal transition agents(PTAs),and carriers of photosensitizer chlorin e6(Ce6)to synthesize a composite nanosystem Pt-Ce6.In this system,Pt-Ce6 can continuously and stably decompose H2O2 into oxygen,thereby alleviating tumor hypoxia and improving the effect of photodynamic therapy(PDT).With 650 nm illumination,the reactive oxygen species(ROS)produced by Ce6 will decrease the mitochondrial membrane potential(MMP,ΔΨm)to release cytochrome c(Cyt-c)from the mitochondria into the cytoplasm,eventually leading to mitochondrial-mediated cellular apoptosis during the PDT process.In addition,Pt-Ce6 has good photothermal stability and high photothermal conversion efficiency(52.62%)in the NIR-II region.In U14 tumor-bearing mice,Pt-Ce6 completely suppressed tumor growth and recurrence under laser irradiation.Thus the nanocomposite shows excellent PDT/photothermal therapy(PTT)synergistic performance in vitro and in vivo.

Electrical conductivity of hydrous silicate melts and aqueous fluids: Measurement and applications

The combination of magnetotelluric survey and laboratory measurements of electrical conductivity is a powerful approach for exploring the conditions of Earth's deep interior. Electrical conductivity of hydrous silicate melts and aqueous fluids is sensitive to composition, temperature, and pressure, making it useful for understanding partial melting and fluid activity at great depths. This study presents a review on the experimental studies of electrical conductivity of silicate melts and aqueous fluids, and introduces some important applications of experimental results. For silicate melts, electrical conductivity increases with increasing temperature but decreases with pressure. With a similar Na^+ concentration, along the calc-alkaline series electrical conductivity generally increases from basaltic to rhyolitic melt, accompanied by a decreasing activation enthalpy. Electrical conductivity of silicate melts is strongly enhanced with the incorporation of water due to promoted cation mobility. For aqueous fluids, research is focused on dilute electrolyte solutions. Electrical conductivity typically first increases and then decreases with increasing temperature, and increases with pressure before approaching a plateau value. The dissociation constant of electrolyte can be derived from conductivity data. To develop generally applicable quantitative models of electrical conductivity of melt/fluid addressing the dependences on temperature, pressure, and composition, it requires more electrical conductivity measurements of representative systems to be implemented in an extensive P-T range using up-to-date methods.