Objective To investigate the regulatory mechanisms of acetylated p53 in the expression of microtubule-associated protein-2(MAP2) in neuronal differentiation of P19 cells induced by all-trans retinoic acid(RA).Methods ...Objective To investigate the regulatory mechanisms of acetylated p53 in the expression of microtubule-associated protein-2(MAP2) in neuronal differentiation of P19 cells induced by all-trans retinoic acid(RA).Methods Neuronal differentiation of P19 cells was initiated with 4-day RA treatment.Immunofluorescence,real-time reverse transcription-polymerase chain reaction(RT-PCR) assay,and map2 promoter driven luciferase assay were performed to detect the expression and relative promoter activity of MAP2 in those RA-treated cells.Real-time PCR-based chromatin immunoprecipitation assay(ChIP) was carried out to reveal the specific recruitment of acetylated p53 onto its binding sites on map2 promoter.Results The expression of MAP2 was markedly increased in RA-induced P19 cells.The map2 mRNA increased 34-fold after 4 days of RA treatment and 730-fold 2 days after the treatment,compared with the cells without RA treatment(control).p53 was recruited to the promoter of map2 gene in acetylated form and thereby enhanced its promoter activity.p300/CBP associated factor(PCAF) was found induced in RA-treated cells and enriched in the nucleus,which might contribute to the acetylation of p53 in the regulation of map2 gene.Conclusions Acetylated p53 may participate in regulating the expression of map2 in RA-induced differentiation of P19 cells.PCAF is possibly involved in this process by mediating the acetylation of p53.展开更多
Taking B2C E-commerce enterprises as the center, it can be divided into a set of logistics activities upstream supply chain logistics producer to B2C e-commerce enterprises, and the B2C e-commerce enterprise logistics...Taking B2C E-commerce enterprises as the center, it can be divided into a set of logistics activities upstream supply chain logistics producer to B2C e-commerce enterprises, and the B2C e-commerce enterprise logistics to client consumer, and the paper focuses on the study of self-supporting logistics for the E-commerce enterprise. According to the development status of current B2C electronic commerce enterprise in our country and logistics, the paper study B2C electric business enterprise how to correctly choose the logistics mode and how to promote the business logistics operation level.展开更多
OBJECTIVE: To investigate the mechanism of Ping-wei capsules(PWC) in improving gastrointestinal m otility in rats with functional dyspepsia(FD).METHODS: We established an FD model by stimu-lating semi-starvation rats ...OBJECTIVE: To investigate the mechanism of Ping-wei capsules(PWC) in improving gastrointestinal m otility in rats with functional dyspepsia(FD).METHODS: We established an FD model by stimu-lating semi-starvation rats via tail damping, provo-cation, and forced exercise fatigue. The FD model group was further divided into five groups accord-ing to the treatment received: normal saline, dom-peridone, low-dose PWC, mid-dose PWC, or highdose PWC. The effect of PWC on FD was evaluated by measuring gastrointestinal motility. Changes in leptin and cholecystokinin(CCK) were detected through enzyme-linked immunosorbent assay, re-verse transcription-polymerase chain reaction, and immunohistochemistry.RESULTS: PWC significantly increased gastrointesti-nal m otility in FD rats. Furthermore, PWC signifi-cantly increased CCK m RNA and protein concentra-tions in the duodenum and antrum, decreased leptin protein concentrations in the duodenum, an-trum, and hypothalamus, and decreased CCK pro-tein concentration in the hypothalamus.CONCLUSION: PWC improves gastrointestinal mo-tor function in FD rats by decreasing the leptin con-centration in serum and the brain-gut axis, and by increasing the CCK concentration in gastrointesti-nal tissue. Our findings help to elucidate the mech-anism of FD and provide further insight into the pharmacokinetics of PWC.展开更多
The prevalence of mobile devices has spurred human mobility to be applied in mobile networking and communications by using network science, in which the temporal evolution of a network topology is of great importance ...The prevalence of mobile devices has spurred human mobility to be applied in mobile networking and communications by using network science, in which the temporal evolution of a network topology is of great importance for protocol design and performance analysis. This paper focuses on link generation in a temporal evolution network. Based on observations revealing the strong correlation between the connection patterns of different time periods, a link generation potential based on historical connections is proposed in this paper, aiming to provide a method for making topological predictions with less randomness. Using MIT Reality dataset, an evaluation of the accuracy of the proposed method was conducted. The experimental results demonstrate the proposal's adequacy in terms of its accuracy.展开更多
基金Supported by National Natural Science Foundation of China (30871382,30721063)National Basic Research Program of China (973 Program) (2005CB522405)Special Funds of State Key Laboratories (2060204)
文摘Objective To investigate the regulatory mechanisms of acetylated p53 in the expression of microtubule-associated protein-2(MAP2) in neuronal differentiation of P19 cells induced by all-trans retinoic acid(RA).Methods Neuronal differentiation of P19 cells was initiated with 4-day RA treatment.Immunofluorescence,real-time reverse transcription-polymerase chain reaction(RT-PCR) assay,and map2 promoter driven luciferase assay were performed to detect the expression and relative promoter activity of MAP2 in those RA-treated cells.Real-time PCR-based chromatin immunoprecipitation assay(ChIP) was carried out to reveal the specific recruitment of acetylated p53 onto its binding sites on map2 promoter.Results The expression of MAP2 was markedly increased in RA-induced P19 cells.The map2 mRNA increased 34-fold after 4 days of RA treatment and 730-fold 2 days after the treatment,compared with the cells without RA treatment(control).p53 was recruited to the promoter of map2 gene in acetylated form and thereby enhanced its promoter activity.p300/CBP associated factor(PCAF) was found induced in RA-treated cells and enriched in the nucleus,which might contribute to the acetylation of p53 in the regulation of map2 gene.Conclusions Acetylated p53 may participate in regulating the expression of map2 in RA-induced differentiation of P19 cells.PCAF is possibly involved in this process by mediating the acetylation of p53.
文摘Taking B2C E-commerce enterprises as the center, it can be divided into a set of logistics activities upstream supply chain logistics producer to B2C e-commerce enterprises, and the B2C e-commerce enterprise logistics to client consumer, and the paper focuses on the study of self-supporting logistics for the E-commerce enterprise. According to the development status of current B2C electronic commerce enterprise in our country and logistics, the paper study B2C electric business enterprise how to correctly choose the logistics mode and how to promote the business logistics operation level.
基金Supported by the Natural Science Foundation of China(Based on Brain-Gut Axis to Study Peptidomics of Liver Stag-nation and Spleen Deficiency with Functional Dyspepsia and Its Intervention of Shugan Jianpi Method,No.8136-0540)the Administration of Gansu Traditional Chinese Medicine and Chinese Herbs(A Study about Intervening Ef-fects of a Chinese Herbal Preparation w ith Resveratrol on In-sulin Resistance of Type 2 Diabetes Mellitus,No.GZK-2015-23)
文摘OBJECTIVE: To investigate the mechanism of Ping-wei capsules(PWC) in improving gastrointestinal m otility in rats with functional dyspepsia(FD).METHODS: We established an FD model by stimu-lating semi-starvation rats via tail damping, provo-cation, and forced exercise fatigue. The FD model group was further divided into five groups accord-ing to the treatment received: normal saline, dom-peridone, low-dose PWC, mid-dose PWC, or highdose PWC. The effect of PWC on FD was evaluated by measuring gastrointestinal motility. Changes in leptin and cholecystokinin(CCK) were detected through enzyme-linked immunosorbent assay, re-verse transcription-polymerase chain reaction, and immunohistochemistry.RESULTS: PWC significantly increased gastrointesti-nal m otility in FD rats. Furthermore, PWC signifi-cantly increased CCK m RNA and protein concentra-tions in the duodenum and antrum, decreased leptin protein concentrations in the duodenum, an-trum, and hypothalamus, and decreased CCK pro-tein concentration in the hypothalamus.CONCLUSION: PWC improves gastrointestinal mo-tor function in FD rats by decreasing the leptin con-centration in serum and the brain-gut axis, and by increasing the CCK concentration in gastrointesti-nal tissue. Our findings help to elucidate the mech-anism of FD and provide further insight into the pharmacokinetics of PWC.
基金supported by the National Natural Science Foundation of China(Grant No.61300183)the National Science Fund for Distinguished Young Scholars in China(Grant No.61425012)
文摘The prevalence of mobile devices has spurred human mobility to be applied in mobile networking and communications by using network science, in which the temporal evolution of a network topology is of great importance for protocol design and performance analysis. This paper focuses on link generation in a temporal evolution network. Based on observations revealing the strong correlation between the connection patterns of different time periods, a link generation potential based on historical connections is proposed in this paper, aiming to provide a method for making topological predictions with less randomness. Using MIT Reality dataset, an evaluation of the accuracy of the proposed method was conducted. The experimental results demonstrate the proposal's adequacy in terms of its accuracy.
