When detecting deletions in complex human genomes,split-read approaches using short reads generated with next-generation sequencing still face the challenge that either false discovery rate is high,or sensitivity is l...When detecting deletions in complex human genomes,split-read approaches using short reads generated with next-generation sequencing still face the challenge that either false discovery rate is high,or sensitivity is low.To address the problem,an integrated strategy is proposed.It organically combines the fundamental theories of the three mainstream methods(read-pair approaches,split-read technologies and read-depth analysis) with modern machine learning algorithms,using the recipe of feature extraction as a bridge.Compared with the state-of-art split-read methods for deletion detection in both low and high sequence coverage,the machine-learning-aided strategy shows great ability in intelligently balancing sensitivity and false discovery rate and getting a both more sensitive and more precise call set at single-base-pair resolution.Thus,users do not need to rely on former experience to make an unnecessary trade-off beforehand and adjust parameters over and over again any more.It should be noted that modern machine learning models can play an important role in the field of structural variation prediction.展开更多
The New Curriculum Standards (NCS) for Middle Schools require students to complete certain amount of English reading as extra-curricular practice at Junior and Senior high levels respectively. Such standards encourage...The New Curriculum Standards (NCS) for Middle Schools require students to complete certain amount of English reading as extra-curricular practice at Junior and Senior high levels respectively. Such standards encourage independent and collaborative learning but may pose a challenge to the test-oriented English teaching in China. Pressed by the graduation and enrollment rates, do teachers still care for organizing extra-curricular reading If yes, are the methods they use effective and scientific To answer these questions, our research team conducted a two-year research in Beijing middle schools. The results show that extra-curricular reading has been implemented differently at different levels to different degrees. Some teachers have worked out feasible, successful methods in accord with international practices, some schools are just starting their experiments, and some have not made plans yet. By analyzing the favored organizing and monitoring methods, and evaluating their benefits and limits, this paper hopes to offer some implications to the researchers with the same endeavor.展开更多
基金Project(61472026)supported by the National Natural Science Foundation of ChinaProject(2014J410081)supported by Guangzhou Scientific Research Program,China
文摘When detecting deletions in complex human genomes,split-read approaches using short reads generated with next-generation sequencing still face the challenge that either false discovery rate is high,or sensitivity is low.To address the problem,an integrated strategy is proposed.It organically combines the fundamental theories of the three mainstream methods(read-pair approaches,split-read technologies and read-depth analysis) with modern machine learning algorithms,using the recipe of feature extraction as a bridge.Compared with the state-of-art split-read methods for deletion detection in both low and high sequence coverage,the machine-learning-aided strategy shows great ability in intelligently balancing sensitivity and false discovery rate and getting a both more sensitive and more precise call set at single-base-pair resolution.Thus,users do not need to rely on former experience to make an unnecessary trade-off beforehand and adjust parameters over and over again any more.It should be noted that modern machine learning models can play an important role in the field of structural variation prediction.
文摘The New Curriculum Standards (NCS) for Middle Schools require students to complete certain amount of English reading as extra-curricular practice at Junior and Senior high levels respectively. Such standards encourage independent and collaborative learning but may pose a challenge to the test-oriented English teaching in China. Pressed by the graduation and enrollment rates, do teachers still care for organizing extra-curricular reading If yes, are the methods they use effective and scientific To answer these questions, our research team conducted a two-year research in Beijing middle schools. The results show that extra-curricular reading has been implemented differently at different levels to different degrees. Some teachers have worked out feasible, successful methods in accord with international practices, some schools are just starting their experiments, and some have not made plans yet. By analyzing the favored organizing and monitoring methods, and evaluating their benefits and limits, this paper hopes to offer some implications to the researchers with the same endeavor.
