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管窥《伤寒论》第六条“温病” 被引量:1
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作者 马坤 庞晓晨 +1 位作者 卢艳 张静莎 《中医学报》 CAS 2021年第4期691-694,共4页
《伤寒论》第六条论述的"温病"与"伤寒"所感邪气相同,均为寒邪。二者发病形式不同,后者感而即发,前者感而不发,至春才病发诸证。温病的发病因春升之阳触动伏寒,邪正交争于外,故而"发热",少阴乏源于内,出... 《伤寒论》第六条论述的"温病"与"伤寒"所感邪气相同,均为寒邪。二者发病形式不同,后者感而即发,前者感而不发,至春才病发诸证。温病的发病因春升之阳触动伏寒,邪正交争于外,故而"发热",少阴乏源于内,出现"虚故饮水自救"之象,故"渴";太阳病极轻,为区别中风、伤寒之恶寒较重,故言"不恶寒"。临证诊断温病应从发病时间、发病的气候特点、症状特点着眼。对于温病的治疗,应通过症状判断太阳之实与少阴之虚孰轻孰重,再定发汗解表与温阳补肾孰先孰后,或如何相兼而行,方可效如桴鼓,不至"变证"迭起。 展开更多
关键词 “温病” 伤寒 《伤寒论》第六条 张仲景
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Study on Pathogenicity Difference of Plasmodiophora brassicae Under Different Temperature and pH Conditions 被引量:1
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作者 裴卫华 李向东 +8 位作者 杨佩文 曹继芬 毕云青 杨子林 芮文 马桂明 林兴华 周丽凤 杨明英 《Agricultural Science & Technology》 CAS 2015年第1期112-115,共4页
[Objective] This study was conducted to investigate the pathogenicity of Plasmodiophora brassicae on cabbage grown under different temperature and soil pH conditions. [Method] The pathogenicity of P. brassicae were te... [Objective] This study was conducted to investigate the pathogenicity of Plasmodiophora brassicae on cabbage grown under different temperature and soil pH conditions. [Method] The pathogenicity of P. brassicae were tested at seven different temperatures and at six different soil pH values with the resting spore concentration of lx108 (spores/g) in the soil. The plant survival rate and incidence rate of clubroot were investigated after 90 d. [Result] The incidence rate of clubroot on cabbage among the different temperature sets varied in a descending order as follows: 30 ℃〉25 ℃〉20 ℃〉35 ℃〉15 ℃〉10 ℃〉5 ℃ at soil pH value of 6, indicating that the pathogenicity of P. brassicae was weak at 5 and 10 ~(3. The incidence rate increased with soil temperature increasing from 15 to 30 ℃, but decreased at 35 ℃. The incidence rates of clubroot were 80.36%, 100%, 65%, 10.77%, 3.23% and 0% at soil pH 4, 5, 6, 7, 8 and 9 at 25 ℃, respectively. The growth of cabbage was inhibited and the survival rate was reduced at pH 4.The incidence rates of clubroot were low at pH value of 7 and 8, and was 0% at pH 9. The Chinese cabbage grew better at pH value of 5 and 6, but had high incidence rates of clubroot. [Conclusion] The results revealed that the incidence rate of clubroot on cabbage was closely related to the temperature and soil pH. 展开更多
关键词 Plasmodiophora brassicae TEMPERATURE pH value PATHOGENICITY
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伤寒与温病的辨析要点 被引量:1
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作者 孙维旭 《中医杂志》 CSCD 北大核心 2022年第21期2033-2036,共4页
伤寒与温病在临床表现上具有一定相似性,尤其是表现为“发热、恶寒、无汗”的伤寒与温病容易造成混淆。通过文献分析并结合临床实践,提出在辨析伤寒与温病时应注意四个要点:第一,应重视伴随症状,如“发热、恶寒、无汗”伴咽喉肿痛、咯... 伤寒与温病在临床表现上具有一定相似性,尤其是表现为“发热、恶寒、无汗”的伤寒与温病容易造成混淆。通过文献分析并结合临床实践,提出在辨析伤寒与温病时应注意四个要点:第一,应重视伴随症状,如“发热、恶寒、无汗”伴咽喉肿痛、咯吐黄痰、胸闷胸痛等呼吸道症状突出者,可考虑为太阴温病,首选清热解毒等治法;如“发热、恶寒、无汗”伴皮肤、骨节疼痛等症状,可考虑为伤寒,首选辛温解表等治法;第二,应充分借助血常规检查、胸部CT等现代医学手段,如有肺炎表现可首先采用太阴温病的清热解毒等治法;第三,应依据疗效调整治疗方案,尤其治疗后效果不佳者需及时调整治疗;第四,伤寒与温病共存的情况治疗则需二者兼顾。 展开更多
关键词 伤寒 吴瑭 条辨
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CLINICAL AND ELECTROPHYSIOLOGICAL OBSERVATIONS ON DIABETIC PERIPHERAL NEUROPATHYTREATED BY WARM NEEDLING AND MOXIBUSTION 被引量:4
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作者 孙远征 徐莺莺 吴迪 《World Journal of Acupuncture-Moxibustion》 2008年第2期1-6,共6页
Objective To observe clinical therapeutic effects of warm needling and moxibustion on diabetic peripheral neuropathy (DPN) and their influence on nerve conduction velocity. Methods Fifty two cases were randomly divi... Objective To observe clinical therapeutic effects of warm needling and moxibustion on diabetic peripheral neuropathy (DPN) and their influence on nerve conduction velocity. Methods Fifty two cases were randomly divided into a treatment group (n =26) and a control group (n =26). In addition to basic treatment for lowering blood sugar in both groups, Pǐshū (BL 20), Shènshū (BL 23), Huántiào (GB 30), Zùsānlǐ (ST 36), Yánglíngquán (GB 34), Sānyīnjiāo (SP 6), Tàixī (KI 3), Qǔchí (LI 11), Wàiguān (TE 5) and Hégǔ (LI 4) were selected for warm needling and moxibustion in the treatment group. Methycobal was intramuscularly injected in the control group. Clinical symptoms and conduction velocities of the tibial nerve and common peroneal nerve were compared before and after treatment. Results Warm needling and moxibustion could alleviate such clinical symptoms as numbness of limbs, pain and hypoesthesia, and obviously improve the conduction velocities of both tibial and common peroneal nerves. Conclusion Warm needling and moxibustion exhibit good therapeutic effects on diabetic peripheral neuropathy. 展开更多
关键词 Diabetic Peripheral Neuropathy Warm Needling and Moxibustion Nerve Conduction Velocity
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Construction of Cucumber Powdery Mildew Early Warning System in Solar Greenhouse Based on Internet of Things 被引量:1
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作者 吕雄杰 王晓蓉 贾宝红 《Agricultural Science & Technology》 CAS 2016年第12期2873-2876,2884,共5页
ln order to explore the design and construction of cucumber powdery mildew warning system in solar greenhouse, internet of things technology was used to conduct the real-time dynamic monitoring of the incidence of cuc... ln order to explore the design and construction of cucumber powdery mildew warning system in solar greenhouse, internet of things technology was used to conduct the real-time dynamic monitoring of the incidence of cucumber powdery mildew and cucumber growth environment in solar greenhouse. The growth environ-ment included temperature and humidity of air and soil. Logistic regression model was used to construct cucumber powdery mildew warning model. The results showed that humidity characteristic variable (maximum air humidity) and temperature characteristic variable (maximum air temperature) had significant effects on the inci-dence probability of cucumber powdery mildew in solar greenhouse. And it was fea-sible to construct cucumber powdery mildew warning system in solar greenhouse with internet of things. 展开更多
关键词 Solar Greenhouse CUCUMBER Powdery Mildew lnternet of Things Warning Model
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Expression and clinical implication of p27 and p57 in cutaneous squamous cell carcinoma and Bowen's disease 被引量:3
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作者 Hong Liang Jiawen Li Dingdan Tian 《The Chinese-German Journal of Clinical Oncology》 CAS 2008年第3期176-178,共3页
Objective: To investigate the expression and clinical significance of cyclin dependent kinase inhibitors p27 and p57 in cutaneous squamous cell carcinoma and Bowen's disease. Methods: The expression of p27 and p57 ... Objective: To investigate the expression and clinical significance of cyclin dependent kinase inhibitors p27 and p57 in cutaneous squamous cell carcinoma and Bowen's disease. Methods: The expression of p27 and p57 were evaluated in 10 normal skin tissues, 25 Bowen's disease (BD) and 30 cutaneous squamous cell carcinoma (SCC) with immunohistochemistrical staining. Results: The levels of p27 protein expression in SCC were dramatically lower than those in normal skin and BD (P 〈 0.057. The levels of p57 protein expression in SCC were dramatically lower than those in normal skin and BD (P 〈 0.057. There was a posilk, e correlation between p27 and p57 (r = 0.469, P 〈 0.057. Conclusion: The decrease of p27 and skp2 may be used for considering the biological behavior of human cutaneous squamous cell carcinoma and Bowen's disease. 展开更多
关键词 P27 P57 cutaneous squamous cell Bowen's disease
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Establishment of Reverse-transcription Loopmediated Isothermal Amplification Method for Detection of Wheat Streak Mosaic Virus 被引量:4
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作者 徐颖 《Agricultural Science & Technology》 CAS 2014年第11期1857-1859,1941,共4页
A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method was established for the detection of wheat streak mosaic virus (WSMV). Ac-cording to the conservative regions of the genes that encod... A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method was established for the detection of wheat streak mosaic virus (WSMV). Ac-cording to the conservative regions of the genes that encode the coat protein of WSMV, 2 pairs of primers were designed. Final y, the 1st pair of primers was select-ed through the specificity test. The sensitivity test showed the sensitivity of RT-LAMP method was 10 times higher than that of RT-PCR. In addition, the amplifica-tion of target gene could be judged visual y from the presence of fluorescence (cal-cein) in the final reaction system. The RT-LAMP method, established in this study, was rapid, easy, specific and sensitive. Moreover, it did not require sophisticated equip-ment. The RT-LAMP was suitable for the rapid detection of WSMV. 展开更多
关键词 Wheat streak mosaic virus (WSMV) Reverse-transcription loop-mediated isothermal amplification (RT-LAMP) Detection method
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The Application of Reverse Transcription-loop-mediated Isothermal Amplification for the Rapid Detection of Maize Chlorotic Dwarf Virus
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作者 徐颖 张峰 +1 位作者 于莹 邱志君 《Agricultural Science & Technology》 CAS 2017年第12期2450-2453,共4页
Maize chlorotic dwarf virus (MCDV) is a quarantine pest as approved by Chinese government. A rapid, sensitive and specific MCDV detection method using reverse transcription-loop-mediated isothermal amplification (R... Maize chlorotic dwarf virus (MCDV) is a quarantine pest as approved by Chinese government. A rapid, sensitive and specific MCDV detection method using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) was estab- lished in this study. Based on the sequence of MCDV coat protein coding gene, specific primers were designed and similar sensitivities were observed between RT- LAMP and RT-PCR, except that RT-LAMP was quicker, and the reaction could be finished within 1 h. In addition, the presence or absence of the fluorescent display in daylight allows naked easy detection of the amplification of MCDV genomic RNA using calcein. The RT-LAMP assay was applied successfully to detect MCDV in maize seeds, and the result by the addition of calcein was consistent with the result detected by the real time turbidimeter. 展开更多
关键词 Maize chlorotic dwarf virus (MCDV) Reverse transcription loop-mediatedisothermal amplification (RT-LAMP) Rapid detection
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A Resistance Bioassay for Rhizoctonia Root and Crown Rot and Damping-off Caused by the Anastomosis Groups AG 2-2111B and AG 4 in Sugar Beet 被引量:4
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作者 Carlos Berdugo Christian Hillnhiitter Richard Sikora Erich-Christian Oerke 《Journal of Agricultural Science and Technology(A)》 2012年第3期294-302,共9页
In order to determine the level of resistance of sugar beet varieties against Rhizoctonia solani AG 2-21IIB and AG 4, a methodology was implemented under greenhouse conditions that contemplated the most important crit... In order to determine the level of resistance of sugar beet varieties against Rhizoctonia solani AG 2-21IIB and AG 4, a methodology was implemented under greenhouse conditions that contemplated the most important criteria regarding to plant-pathogen interaction. The effect of plant growth stage on the development of the disease was evaluated. Seven sugar beet varieties were tested for resistance to R. solani AG 2-2IIIB and AG 4. To detect differences in leaf temperature between/L solani inoculated plants and non-infected plants, an infrared (IR) camera was tested. High incidence of R. solani AG 2-2IIIB and AG 4 in sugar beet plants was evident when the fungal inoculum was applied to two and four weeks old plants. At four weeks after sowing, it was the optimum time to inoculate sugar beet plants in order to generate R. solani infection, since at this time all plants were infected. Significant differences were detected regarding disease incidence between sugar beet varieties inoculated with different anastomosis groups. Leaf temperature was significant different between inoculated and non-inoculated plants, demonstrated that this technique could be a new tool for breeders to screen for resistance of new varieties. 展开更多
关键词 Beta vulgaris inoculation IR-thermal imaging greenhouse test soil-borne pathogen tolerant varieties.
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A REVIEW ON TRADITIONAL CHINESE MEDICINE IN PREVENTION AND TREATMENT OF MULTIPLE SCLEROSIS 被引量:1
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作者 孙怡 刘晓艳 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 1999年第1期65-73,共9页
Multiple sclerosis(MS),a diseaseaffecting the central nervous system,ischaracterized by patches of demyelinationand sclerosis of gliosis desseminatedthroughout the white matter of brain andspinal cord.In 1968,Charcot ... Multiple sclerosis(MS),a diseaseaffecting the central nervous system,ischaracterized by patches of demyelinationand sclerosis of gliosis desseminatedthroughout the white matter of brain andspinal cord.In 1968,Charcot firstdescribed its clinical and pathologicalcharacteristics.Its incidence is very high,especially in Europe and America.Thereis no epidemic data of this 展开更多
关键词 ANIMALS Diagnosis Differential Drugs Chinese Herbal Humans Medicine Chinese Traditional Multiple Sclerosis
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Smartphone-Imaged HIV-1 Reverse-Transcription Loop-Mediated Isothermal Ampliflcation(RT-LAMP) on a Chip from Whole Blood 被引量:9
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作者 Gregory L.Damhorst Carlos Duarte-Guevara +3 位作者 Weili Chen Tanmay Ghonge Brian T.Cunningham Rashid Bashir 《Engineering》 SCIE EI 2015年第3期324-335,共12页
Viral load measurements are an essential tool for the long-term clinical care of human immunodeficiency virus (HIV)-positive individuals. The gold standards in viral load instrumentation, however, are still too limi... Viral load measurements are an essential tool for the long-term clinical care of human immunodeficiency virus (HIV)-positive individuals. The gold standards in viral load instrumentation, however, are still too limited by their size, cost, and sophisticated operation for these measurements to be ubiquitous in remote settings with poor healthcare infrastructure, including parts of the world that are disproportionately affected by HIV infection. The challenge of developing a point-of-care platform capable of making viral load more accessible has been frequently approached but no solution has yet emerged that meets the practical requirements of low cost, portability, and ease-of-use. In this paper, we perform reverse-transcription loop-mediated isothermal amplification (RT-LAMP) on minimally processed HIV-spiked whole blood samples with a microfluidic and silicon microchip platform, and perform fluorescence measurements with a consumer smartphone. Our integrated assay shows amplification from as few as three viruses in a - 60 nL RT- LAMP droplet, corresponding to a whole blood concentration of 670 viruses per μL of whole blood. The technology contains greater power in a digital RT-LAMP approach that could be scaled up for the determination of viral load from a finger prick of blood in the clinical care of HIV-positive individuals. We demonstrate that all aspects of this viral load approach, from a drop of blood to imaging the RT-LAMP reaction, are compatible with lab-on-a-chip components and mobile instrumentation. 展开更多
关键词 human immunodeficiency virus (HIV) viral load loop-mediated isothermal amplification SMARTPHONE POINT-OF-CARE
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Prevention of core cell damage in isolated islets of Langerhans by low temperature preconditioning 被引量:1
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作者 Yun-FuCui MingMa +3 位作者 Gui-YuWang De-EnHan BrigitteVollmar MichaelD.Menger 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第4期545-550,共6页
AIM: To study the core cell damage in isolated islets of Langerhans and its prevention by low temperature preconditioning (26 ℃).METHODS: Islets were cultured at 37 ℃ for 7-14 d after isolation, and then at 26 ℃ fo... AIM: To study the core cell damage in isolated islets of Langerhans and its prevention by low temperature preconditioning (26 ℃).METHODS: Islets were cultured at 37 ℃ for 7-14 d after isolation, and then at 26 ℃ for 2, 4 and 7 d before additional culture at 37 ℃ for another 7 d. Core cell damage in the isolated islets was monitored by video-microscopy and analyzed quantitatively by use of a computer-assisted image analysis system. The analysis included daily measurement of the diameter and the area of the isolated islets and the area of the core cell damage that developed in those islets over time during culture. Histology and TdT-mediated dUTP-biotin nick end labeling (TUNEL) assay were used to characterize the cell damage and to monitor islet function.RESULTS: Microscopic analysis showed that during the 7 to 14 d of culture at 37 ℃, core cell damage occurred in the larger islets with diameters >200 μm, which included both necrotic and apoptotic cell death. Low temperature (26 ℃) culture could prevent core cell damage of isolated islets. The 7-d culture procedure at 26 ℃ could inhibit most of the core cell (excluding diameters>300 μm) damages when the islets were re-warmed at 37 ℃.CONCLUSION: Our results indicate that core cell damage within isolated islets of Langerhans correlates with the size of islets. Low temperature (26 ℃) culture can prevent core cell damage in isolated islets, and successfully precondition these islets for incubation at 37 ℃. These novel findings may help to understand the pathophysiology of early loss of islet tissue after transplantation, and may provide a new strategy to improve graft function in the clinical setting of islet transplantation. 展开更多
关键词 Islets of Langerhans Low temperature preconditioning Core cell damage
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Assessment of the psychological distress difficulties in patients with cancer using the national comprehensive cancer network rapid screening measure
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作者 Hamid Saeedi Saedi Mona Koochak Pour +1 位作者 Emad Sabahi Soodabeh Shahidsales 《The Chinese-German Journal of Clinical Oncology》 CAS 2012年第8期472-477,共6页
Objective Clinical guidelines like National Comprehensive Cancer Network Disease recommend routine psychological distress screening as a common problem among patients with cancer. The purpose of this study was to ass... Objective Clinical guidelines like National Comprehensive Cancer Network Disease recommend routine psychological distress screening as a common problem among patients with cancer. The purpose of this study was to assess the prevalence of clinically significant emotional distress related to demographic and clinical association by standard distress thermometer (DT) within the patients lived in different regions of Gilan state, Iran. 展开更多
关键词 DISTRESS SCREENING distress thermometer malignant diseases
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Development of a Loop-Mediated Isothermal Amplification Assay for Porcine Circovirus Type 2 被引量:3
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作者 Ye-bing Liu Lei Zhang +2 位作者 Qin-hong Xue Yi-bao Ning Zhi-gang Zhang 《Virologica Sinica》 SCIE CAS CSCD 2011年第3期214-220,共7页
In this study, the loop-mediated isothermal amplification (LAMP) method was used to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). According to the PCV2 sequences published in GenBan... In this study, the loop-mediated isothermal amplification (LAMP) method was used to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). According to the PCV2 sequences published in GenBank, multiple LAMP primers were designed targeting conserved sequences of PCV2. Using the DNA extracted from PCV2 isolates HUN-09 and SD-09 as the template, LAMP reactions in a PCV2 LAMP system was performed, the amplification products were detected by adding SYBR Green I and could be observed directly by the naked eye. The results showed highly-efficient and specific amplification in 30 min at 63°C with a LAMP real-time turbidimeter. Furthermore, PCV2 DNA templates, with a detection limit of 5.5×10-5 ng of nucleic acid, indicated that this assay was highly sensitive. The results obtained with the naked eye after SYBR Green I staining were consistent with those detected by the real-time turbidimeter, showing the potential simplicity of interpretation of the assay results. The LAMP assay appeared to have greater accuracy than PCR and virus isolation for the analysis of 18 clinical samples. In addition it offers higher specificity and sensitivity, shorter reaction times and simpler procedures than the currently available methods of PCV2 detection. It is therefore a promising tool for the effective and efficient detection of PCV2. 展开更多
关键词 Porcine circovirus type 2 (PCV2) Loop-mediated isothermal amplification (LAMP) Virus detection
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A meta-analysis of Wendan decoction in the treatment of diabetic gastroparesis
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作者 Shuai Chen Shuqin Zheng 《TMR Clinical Research》 2019年第2期82-90,共9页
Objective To evaluate the clinical efficacy and safety of Wendan decoction in the treatment of diabetic gastroparesis (DGP). Methods PubMed, MEDLINE, The Cochrane Library, Embase, China Biology Medicine (CBM), Chinese... Objective To evaluate the clinical efficacy and safety of Wendan decoction in the treatment of diabetic gastroparesis (DGP). Methods PubMed, MEDLINE, The Cochrane Library, Embase, China Biology Medicine (CBM), Chinese National Knowledge Infrastructure (CNKI), China Science and Technology Journal Database (VIP) and Wanfang databases were searched and eligible randomized controlled clinical trials (RCTs) were included. The quality of the RCTs was evaluated by the Cochrane risk of bias assessment tool, and data analysis were performed with Review Manager 5.3. Results A total of 7 studies were included, including 533 patients. Meta analysis results showed that the effective rate of Wendan decoction in the treatment of diabetic gastroparesis was better than that of the control group [RR=1.30, 95%CI (1.20, 1.40), P<0.001], and the improvement of fasting blood glucose (FBG) was better than that of western medicine [MD =-0.43, 95%CI (-0.91,-0.01), P=0.05]. The recurrence rate in the TCM treatment group was lower than that in the western medicine group[RR = 0.29, 95%CI (0.13, 0.62), P=0.002]. Conclusion Compared with western medicine, Wendan decoction has better curative effect on DGP and can reduce its recurrence rate. However, due to the low quality of the included literatures and the possibility of bias, large-scale and high-quality RCTs are still needed for further confirmation. 展开更多
关键词 Wendan decoction Diabetic gastroparesis Effective rate SYMPTOMS Meta-anaysis
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Molecular mapping for seedling cold tolerance QTLs in rice
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作者 ZhanQingcai SaidoKoji KatoAkira 《Hunan Agricultural Science & Technology Newsletter》 2003年第1期8-15,共8页
The quantitative trait loci (QTLs) for cold tolerance relative characters were identified with microsatellite markers.Ten QTLs located on chromosome 1, 3, 4, 5, 6, 8, 9, 11(two)and 12 were detected for seedling height... The quantitative trait loci (QTLs) for cold tolerance relative characters were identified with microsatellite markers.Ten QTLs located on chromosome 1, 3, 4, 5, 6, 8, 9, 11(two)and 12 were detected for seedling height at different low temperature. Only 2 of these were detected at the same locus at four environments, 1 was significant at three environments, 6 were significant at two environments and 1 was significant at one environment. Seven QTLs located on chromosome 1(two), 2(two),5,6,8 were found for low temperature chlorosis resistance and five QTLs located on chromosome 3, 4, 7, 8, 11 resistant to chilling injury. The amount of variation explained by individual QTL ranged from 4.85%to 49.34%. There was no linkage relationship among the three characters, which indicates seedling cold tolerance is a complex character and is controlled by different QTLs. 展开更多
关键词 RICE seedling cold tolerance microsatellite marker QTLS
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Construction and Genetic Analysis of Murine Hepatitis Virus Strain A59 Nsp16 Temperature Sensitive Mutant and the Revertant Virus
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作者 Guo-hui Chang Bao-jun Luo +5 位作者 Pin Lu Lei Lin Xiao-yan Wu Jing Li Yi Hu Qing-yu Zhu 《Virologica Sinica》 SCIE CAS CSCD 2011年第1期19-29,共11页
Coronaviruses (CoVs) are generally associated with respiratory and enteric infections and have long been recognized as important pathogens of livestock and companion animals. Mouse hepatitis virus (MHV) is a widely st... Coronaviruses (CoVs) are generally associated with respiratory and enteric infections and have long been recognized as important pathogens of livestock and companion animals. Mouse hepatitis virus (MHV) is a widely studied model system for Coronavirus replication and pathogenesis. In this study,we created a MHV-A59 temperature sensitive (ts) mutant Wu"-ts18(cd) using the recombinant vaccinia reverse genetics system. Virus replication assay in 17C1-1 cells showed the plaque phenotype and replication characterization of constructed Wu"-ts18(cd) were indistinguishable from the reported ts mutant Wu"-ts18. Then we cultured the ts mutant Wu"-ts18(cd) at non-permissive temperature 39.5°C,which "forced" the ts recombinant virus to use second-site mutation to revert from a ts to a non-ts phenotype. Sequence analysis showed most of the revertants had the same single amino acid mutation at Nsp16 position 43. The single amino acid mutation at Nsp16 position 76 or position 130 could also revert the ts mutant Wu"-ts18 (cd) to non-ts phenotype,an additional independent mutation in Nsp13 position 115 played an important role on plaque size. The results provided us with genetic information on the functional determinants of Nsp16. This allowed us to build up a more reasonable model of CoVs replication-transcription complex. 展开更多
关键词 Genetic analysis MHV-A59 Temperature-sensitive mutant Revertant Nonstructural proteins (Nsp)
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Effects of Fungicide Treatments and Storage Temperatures on Fruit Quality of Two Mango (Mangifera indica) Cultivars
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作者 Albert Addo-Quaye George Nkansah +2 位作者 Elvis Asare-Bediako Bertina Nyame Joel Asiedu 《Journal of Agricultural Science and Technology(A)》 2013年第7期511-520,共10页
Harvested fruits of mango (Mangifera indica) suffer from both physiological and pathological deterioration in quality during storage which consequently affects their market value. A study was conducted to investigat... Harvested fruits of mango (Mangifera indica) suffer from both physiological and pathological deterioration in quality during storage which consequently affects their market value. A study was conducted to investigate the effects of fungicide treatments and storage temperatures on fruit quality of two mango cultivars, Keirt and Palmer. Mango fruits were treated with 0.04% or 0.08% v/v Triadimefon at either 53 ℃ (hot) or at room temperature and then either stored under ambient conditions (27-29 ℃) or in a refrigerator at a temperature of 11-12 ℃. Fruits stored under ambient conditions developed external colour faster than those stored at a lower temperature. Brix values for fruits treated with Triadimefon decreased with time. Fruits stored at lower temperatures gave least Brix values as compared with those stored under ambient conditions. Percentage titratable acid (%TA) of fruits stored at low temperatures increased over time. Fruits stored at lower temperatures recorded lower pH compared with fruits stored under ambient conditions. Palmer fruits recorded higher %Brix to %TA ratio, indicating a higher sweetness level. The firmness of Palmer fruits kept at lower temperatures increased with time when compared with Keitt fruit. Fruits treated with hot water at 53 ℃ and/or stored at low temperatures (11-12 ℃) had lower levels of anthracnose infection than other treatments. Keitt fruits were more susceptible to anthracnose infection than Palmer fruits. Mango fruits should therefore be treated with hot water at 53 ℃ and then stored at low temperatures (11-12 ℃) in order to slow down the rate of ripening and to reduce anthracnose infection. 展开更多
关键词 TRIADIMEFON MANGO storage temperature fruit quality anthracnose.
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Detection of Potato Spindle Tuber Viroid, and Its Impact on Growth, Production of Tomatoes in Greenhouse
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作者 Haniyeh Mohamed Algamodi Jaber Abdullah Khalil +1 位作者 Taher Ahmed Abouhliqh Khaled Ojdj 《Journal of Agricultural Science and Technology(B)》 2012年第8期940-949,共10页
The viability of most tomato varieties cultivated in Libya have been tested to infect with potato spindle tuber viroid/potatoes (PSTVd) and its impact on growth and production of some of these varieties, which were ... The viability of most tomato varieties cultivated in Libya have been tested to infect with potato spindle tuber viroid/potatoes (PSTVd) and its impact on growth and production of some of these varieties, which were mechanically inoculated with Libyan isolate of viroid PSTVd as follows: Vlkato, Sankarh, Lebda, Jasmine, Kenza and Hana. The percent of incidence were 95.95%, 90%, 90.80%, 80% and 20%, respectively. The following varieties have been contagious mechanically with viroid of PSTVd: Vlkato, zahra, Toria, Lebda, Hoda, Farwa, Alkaraz, Naziha, Rim Star and Kartika. The percent of incidence were 95.95%, 85%, 85.80%, 80%, 70.40%, 0.0%, 0.0%, respectively. The varied symptoms of wrinkle, twist, warp, swell the veins of the leaves, dark brown spots formation and a large yellow spots turned into white patches. Also the effect of the Egyptian isolate viroid PSTVd in the growth and production of varieties Jasmine, Lebda, Soberhalim, and treasure No. 185 had been studied, as the average rates of decline in the production of the fruits tomatoes/tomato 43.4% and 17% length of plants, and in the fresh weight and dry root of the sum of 35% and 37% respictively. 展开更多
关键词 Potato spindle tuber viroid the infection of tomato plants nucleic acid PCR.
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Reverse Transcription-loop-mediated Isothermal Amplification for Detection of Maize Chlorotic Mottle Virus 被引量:4
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作者 Ying XU Yafei XU +2 位作者 Yongfeng LIU Zhijun QIU Wei ZHENG 《Agricultural Science & Technology》 CAS 2017年第1期123-126,共4页
Maize chlorotic mottle virus (MCMV) is a quarantine pest as approved by Chinese government: A rapid, sensitive and specific MCMV detection method using reverse transcription-loop-mediated isothermal amplification ... Maize chlorotic mottle virus (MCMV) is a quarantine pest as approved by Chinese government: A rapid, sensitive and specific MCMV detection method using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) was established in this study. Based on the sequence of MCMV coat protein coding gene, 3 sets of primers were designed and specificity test showed that the second set of primers was specific to MCMV, Similar sensitivities were observed on RT-LAMP and RT-PCR, except that RT-LAMP was quicker, and the reaction could be finished within 1 h. In addition, the presence or absence of the fluorescence under daylight allows naked easy detection of the amplification of MCMV genomic RNA using calcein. The RT-LAMP assay was applied successfully to detect MCMV in maize seeds, and the result by the addition of calcein was consistent with the result detected by the real time turbidimeter. The method is rapid, specific, sensitive without the need for complicated equipment, and is suitable for rapid field detection of MCMV. 展开更多
关键词 Maize chlorotic mottle virus (MCMV) Reverse transcription loop-mediated isothermal amplification (RT-LAMP) DETECTION
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