Acyl-coenzyme A dehydrogenases （ACAD） are a family of nuclear-coded, mitochondrial flavoenzymes that catalyze the alpha, and beta-dehydrogenation of fatty acids. The eighth member of this family, ACAD8 catalyzes the...Acyl-coenzyme A dehydrogenases （ACAD） are a family of nuclear-coded, mitochondrial flavoenzymes that catalyze the alpha, and beta-dehydrogenation of fatty acids. The eighth member of this family, ACAD8 catalyzes the valine catabolism. In this study, the bovine ACAD8 full-length mRNA and genomic DNA sequence were obtained and its gene structure was determined through alignment of the genomic DNA sequence to the mRNA sequence. The mRNA sequence consisted of a 1,251 bp open reading frame （ORF） flanked by a 37 bp 5＇-untranslated region （UTR） and a 444 bp 3＇-UTR; and its full-length genomic DNA sequence was 13,814 bp in length and included 11 exons and 10 introns. One A-G single nucleotide polymorphism （SNP） was revealed at nucleotide 13,408 （GenBank accession No. DQ435445） in the bovine ACAD8 gene by sequencing the polymerase chain reaction （PCR） products of 6 randomly selected individuals from the sample population. Different genotypes were determined by restriction fragment length polymorphism （RFLP）. The association analysis of this SNP in bovine ACAD8 with production traits in 178 unrelated steers from 5 breeds showed that it had a significant effect on the daily gain and the beef tenderness （P〈0.05）. Cattle with the G allele grew more rapidly and the beef they produced was more tender than those with the A allele. Thus, this SNP of the bovine ACAD8 gene can be used as an indicator to improve the growth rate and the beef tenderness.展开更多
[Objective] The study was to explore the major factors affecting diary cattle brucellosis risk assessment,as well as their strong-to-weak sequence,so as to provide theoretical basis for assessing diary cattle brucello...[Objective] The study was to explore the major factors affecting diary cattle brucellosis risk assessment,as well as their strong-to-weak sequence,so as to provide theoretical basis for assessing diary cattle brucellosis risk level in different regions.[Method] From 4 dimensions of feeding and importing,breeding,housing and polyculture situation,an evaluation index system was set up,and diary cattle brucellosis risk survey was conducted in 3 typical regions.Finally,systematic multilevel grey relation entropy method was applied to perform data analysis.[Result] The strong-to-weak sequence of Level 1 impact factor of diary cattle brucellosis was as follows：feeding and importinghousingpolyculture situationbreeding;the sequence of Level 2 impact factor was U32〉U12〉U11〉U31〉U21〉U42〉U43〉U23〉U22〉U41;the risk level sequence of 3 typical regions was Province A（County A1,A2,A3）Province B（County B1,B2,B3）Province C（County C1,C2,C3）.[Conclusion] According to the weight of Level 1 index strata,administrative departments at all levels and dairy cattle farmers should lay emphasis on the aspects of feeding,importing and housing;viewed from the perspective of Level 2 index strata,dairy cattle farmers should value the siting of cattle field,the brucellosis surveillance before importing and milking modes most.According to the diary cattle brucellosis risk level of 3 typical regions,if administrative departments at all levels strengthen peoples＇ awareness of their personal health and increase investment in this area,with new healthy cultured atmosphere built,the risk level of diary cattle brucellosis will surly decline.展开更多
A high affinity polyclonal antibody-based enzyme linked immunosorbent assay （ELISA） was developed for the quantification of zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution facto...A high affinity polyclonal antibody-based enzyme linked immunosorbent assay （ELISA） was developed for the quantification of zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution factors producing insignificant matrix interference were selected as 1：5 in pretreatment. In the improved ELISA, the linear response range was between 0.02 and 1 μg/ml, and the detection limit was 0.02 μg/ml for the assay. The overall recoveries and the coefficients of variation （CVs） were in the range of 82%-127% and 3.5%-8.8%, respectively. Thirty-six bovine urine samples spiked with zeranol （ranging from 0.2 to 10 μg/ml） were detected by the ELISA and liquid chromatography （LC） method, and good correlations were obtained between the two methods （R^2=0.9643）. We conclude that this improved ELISA is suitable tool for a mass zeranol screening and can be an altemative for the conventional LC method for zeranol in bovine urine.展开更多
We read with great interest the recent review by Rodrigo on the celiac disease （CD）. The author analyzed all aspects of CD. Contrary to common belief, this disorder is a systemic disease, rather than a pure digestiv...We read with great interest the recent review by Rodrigo on the celiac disease （CD）. The author analyzed all aspects of CD. Contrary to common belief, this disorder is a systemic disease, rather than a pure digestive alteration. In fact, CD is associated with several diseases： skin manifestations, endocrine disorders, iron-deficiency anemia, osteoporosis, hypertransaminasemia, neurologic disorders and cancer. In the description of skin manifestations, the author reported the association between CD and dermatitis herpetiformis （DH）. This condition affects about 15%-25% of patients with CD. Antibodies for CD have been detected in patients with DH. As CD and DH may be vastly different, they both share a unique intestinal sensitivity to gluten. The rash of DH is thought to be an external marker of the underlying intestinal sensitivity that is likely to be the result of molecular mimicry between the auto-antigen tissue transglutaminase resident in the gut and the skin derived epidermal transglutaminase[3]. However, our study group has reported all CD-associated skin manifestations described in the literature, and in particular psoriasis.展开更多
Based on the new metric theory of gravitation suggested by the author of this article, it gives a possible theoretical interpretation on the famous experiment done by D.R. Long in 1976, i.e. the distance-dependent eff...Based on the new metric theory of gravitation suggested by the author of this article, it gives a possible theoretical interpretation on the famous experiment done by D.R. Long in 1976, i.e. the distance-dependent effect of the gravitational constant in Newton's theory of gravitation.展开更多
The study used a naturally occurring crosslinking reagent-genipin to chemically modify acellular bovine pericardium, prepare cardiac valve tissue engineering scaffold material,and evaluated genipin crosslinked acellul...The study used a naturally occurring crosslinking reagent-genipin to chemically modify acellular bovine pericardium, prepare cardiac valve tissue engineering scaffold material,and evaluated genipin crosslinked acellular matrix of bovine pericardium by investigating the physical and chemical properties of the tissues, such as the surface properties, crosslinking characteristics, mechanical properties, resistance to enzymatic capacity in vitro, and hemolysis tests. The results showed that acellular bovine pericardium matrix erosslinked with genipin was strong hydrophilicity, high crosslinking index, and stable structure, which can maintain good mechanical properties. As a kind of scaffold material for valve tissue engineering, it has wide application prospect.展开更多
After entering to the Europe Union, Slovak Republic has implemented the EU legislation into the legislation of the Slovak Republic. The previous legislation of this country was effective in practice. So we were intere...After entering to the Europe Union, Slovak Republic has implemented the EU legislation into the legislation of the Slovak Republic. The previous legislation of this country was effective in practice. So we were interested in, whether actual legislation is effective in practice too as previous or it will be necessary to make some changes in the legislation. This led us to carry out the assessment of welfare by farmed animal, specifically by calves. Our assessment was based on the Bristol Welfare Assurance Programme protocol developed at the University of Bristol. Very good results were achieved by assessment, and it shows about good level of legislation in the area of protection of animals, it has conditions and requirements, which will not require big changes and the breeder can ensure without bigger problems so to ensure to animals their five freedoms originally established by the Brambell Committee in 1965： （1） Freedom from hunger and thirst; （2） Freedom from discomfort; （3） Freedom fi＇om pain, injury or disease; （4） Freedom to express normal behaviour; （5） Freedom from fear and distress.展开更多
The rapid pace of development of GIS （geographical information system） has assisted in identification of conservation priority sites by delineating species distribution using models on habitat suitability. Gaur, Bos...The rapid pace of development of GIS （geographical information system） has assisted in identification of conservation priority sites by delineating species distribution using models on habitat suitability. Gaur, Bos gaurus, is categorized as ＂Vulnerable＂ in the IUCN Red List of Threatened Species, 2009. The study has used ENFA （ecological niche factor analysis） to understand the distribution status of Gaur in TATR （Tadoba-Andhari Tiger Reserve）, Central India. TATR was sampled using stratified random sampling strategy. A total of 21 continuous variables were used, categorised under 4 environmental descriptors categories viz. habitat, anthropogenic, topographic and hydrological variables. All the variables were tested for the correlation and one of the variable among strongly correlated （r 〉 0.7） variables was discarded to avoid redundancy. A total of 14 variables were retained. The model resulted in marginality of 0.56 and specialization of 2.608. Presence of Gaur showed the positive association with canopy density classes （〈 30% ＆ 40-60%） and open forest. However, it was negatively associated with elevation, non-forest, riparian forest, scrub and teak forest. The study has delineated the areas where appropriate habitat conditions exist to sustain Gaur populations vital for planning strategies for conservation of this megaherbivore species in tropical forests.展开更多
The objectives of the present study were to determine associations between these polymorphisms of PON1 gene and growth and carcass traits. For this purpose, genotyping was performed on males of 18 Angus, 23 Jinnan cat...The objectives of the present study were to determine associations between these polymorphisms of PON1 gene and growth and carcass traits. For this purpose, genotyping was performed on males of 18 Angus, 23 Jinnan cattle, 20 Limousin, 28 Luxi cattle, 26 Qinchuan cattle, 29 Simmental, 29 Charolais. Traits of interest were analyzed using the RFLP-PCR and GLM procedure of SAS 9.1 and least square means of the genotypes were compared by the Tukey＇ s test in the association studies. Association of PONI/ EcoRV genotypes with body weight, average daily gain, rib eye area and tenderness were significant （P〈0.05）, and AG genotype was significant difference with others in average daily gain and tenderness （P〈0.05）, with AA genotype may have higher rib eye area （p〈0.05）. The association analysis of the PONI/Alu I polymorphism showed significant associations between genotypes and growth： body weight and carcass traits net meat weight and tenderness （P〈0.05）. AA genotype was considered more favorable than others in all growth and production traits. There were significant differences among breeds for all associated interests traits and difference between beef breeds and native breeds are obviously in all aspects. And these results may be useful for the breeding selection.展开更多
基金This work was supported by Chinese National Programs for High Technology Research and Development (No. 2002AA242011)the 10th Five Years Key Problems for Science and Technology Development of China (No. 2002BA518A14).
文摘Acyl-coenzyme A dehydrogenases （ACAD） are a family of nuclear-coded, mitochondrial flavoenzymes that catalyze the alpha, and beta-dehydrogenation of fatty acids. The eighth member of this family, ACAD8 catalyzes the valine catabolism. In this study, the bovine ACAD8 full-length mRNA and genomic DNA sequence were obtained and its gene structure was determined through alignment of the genomic DNA sequence to the mRNA sequence. The mRNA sequence consisted of a 1,251 bp open reading frame （ORF） flanked by a 37 bp 5＇-untranslated region （UTR） and a 444 bp 3＇-UTR; and its full-length genomic DNA sequence was 13,814 bp in length and included 11 exons and 10 introns. One A-G single nucleotide polymorphism （SNP） was revealed at nucleotide 13,408 （GenBank accession No. DQ435445） in the bovine ACAD8 gene by sequencing the polymerase chain reaction （PCR） products of 6 randomly selected individuals from the sample population. Different genotypes were determined by restriction fragment length polymorphism （RFLP）. The association analysis of this SNP in bovine ACAD8 with production traits in 178 unrelated steers from 5 breeds showed that it had a significant effect on the daily gain and the beef tenderness （P〈0.05）. Cattle with the G allele grew more rapidly and the beef they produced was more tender than those with the A allele. Thus, this SNP of the bovine ACAD8 gene can be used as an indicator to improve the growth rate and the beef tenderness.
基金Supported by Special Research Fund for Public Sector(Agriculture)(200903055)~~
文摘[Objective] The study was to explore the major factors affecting diary cattle brucellosis risk assessment,as well as their strong-to-weak sequence,so as to provide theoretical basis for assessing diary cattle brucellosis risk level in different regions.[Method] From 4 dimensions of feeding and importing,breeding,housing and polyculture situation,an evaluation index system was set up,and diary cattle brucellosis risk survey was conducted in 3 typical regions.Finally,systematic multilevel grey relation entropy method was applied to perform data analysis.[Result] The strong-to-weak sequence of Level 1 impact factor of diary cattle brucellosis was as follows：feeding and importinghousingpolyculture situationbreeding;the sequence of Level 2 impact factor was U32〉U12〉U11〉U31〉U21〉U42〉U43〉U23〉U22〉U41;the risk level sequence of 3 typical regions was Province A（County A1,A2,A3）Province B（County B1,B2,B3）Province C（County C1,C2,C3）.[Conclusion] According to the weight of Level 1 index strata,administrative departments at all levels and dairy cattle farmers should lay emphasis on the aspects of feeding,importing and housing;viewed from the perspective of Level 2 index strata,dairy cattle farmers should value the siting of cattle field,the brucellosis surveillance before importing and milking modes most.According to the diary cattle brucellosis risk level of 3 typical regions,if administrative departments at all levels strengthen peoples＇ awareness of their personal health and increase investment in this area,with new healthy cultured atmosphere built,the risk level of diary cattle brucellosis will surly decline.
基金Project supported by the National Natural Science Foundation of China (No. 30471155) the Agriculture Key Technologies R & D Program of Shanghai (No. (2003) 9-4), China
文摘A high affinity polyclonal antibody-based enzyme linked immunosorbent assay （ELISA） was developed for the quantification of zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution factors producing insignificant matrix interference were selected as 1：5 in pretreatment. In the improved ELISA, the linear response range was between 0.02 and 1 μg/ml, and the detection limit was 0.02 μg/ml for the assay. The overall recoveries and the coefficients of variation （CVs） were in the range of 82%-127% and 3.5%-8.8%, respectively. Thirty-six bovine urine samples spiked with zeranol （ranging from 0.2 to 10 μg/ml） were detected by the ELISA and liquid chromatography （LC） method, and good correlations were obtained between the two methods （R^2=0.9643）. We conclude that this improved ELISA is suitable tool for a mass zeranol screening and can be an altemative for the conventional LC method for zeranol in bovine urine.
文摘We read with great interest the recent review by Rodrigo on the celiac disease （CD）. The author analyzed all aspects of CD. Contrary to common belief, this disorder is a systemic disease, rather than a pure digestive alteration. In fact, CD is associated with several diseases： skin manifestations, endocrine disorders, iron-deficiency anemia, osteoporosis, hypertransaminasemia, neurologic disorders and cancer. In the description of skin manifestations, the author reported the association between CD and dermatitis herpetiformis （DH）. This condition affects about 15%-25% of patients with CD. Antibodies for CD have been detected in patients with DH. As CD and DH may be vastly different, they both share a unique intestinal sensitivity to gluten. The rash of DH is thought to be an external marker of the underlying intestinal sensitivity that is likely to be the result of molecular mimicry between the auto-antigen tissue transglutaminase resident in the gut and the skin derived epidermal transglutaminase[3]. However, our study group has reported all CD-associated skin manifestations described in the literature, and in particular psoriasis.
文摘Based on the new metric theory of gravitation suggested by the author of this article, it gives a possible theoretical interpretation on the famous experiment done by D.R. Long in 1976, i.e. the distance-dependent effect of the gravitational constant in Newton's theory of gravitation.
基金National High-tech Research and Development Program(836 Prgoram) of Chinagrant number:2007AA02Z444+1 种基金National BasicProgram of China (973 Program)grant number:2009CB930000
文摘The study used a naturally occurring crosslinking reagent-genipin to chemically modify acellular bovine pericardium, prepare cardiac valve tissue engineering scaffold material,and evaluated genipin crosslinked acellular matrix of bovine pericardium by investigating the physical and chemical properties of the tissues, such as the surface properties, crosslinking characteristics, mechanical properties, resistance to enzymatic capacity in vitro, and hemolysis tests. The results showed that acellular bovine pericardium matrix erosslinked with genipin was strong hydrophilicity, high crosslinking index, and stable structure, which can maintain good mechanical properties. As a kind of scaffold material for valve tissue engineering, it has wide application prospect.
文摘After entering to the Europe Union, Slovak Republic has implemented the EU legislation into the legislation of the Slovak Republic. The previous legislation of this country was effective in practice. So we were interested in, whether actual legislation is effective in practice too as previous or it will be necessary to make some changes in the legislation. This led us to carry out the assessment of welfare by farmed animal, specifically by calves. Our assessment was based on the Bristol Welfare Assurance Programme protocol developed at the University of Bristol. Very good results were achieved by assessment, and it shows about good level of legislation in the area of protection of animals, it has conditions and requirements, which will not require big changes and the breeder can ensure without bigger problems so to ensure to animals their five freedoms originally established by the Brambell Committee in 1965： （1） Freedom from hunger and thirst; （2） Freedom from discomfort; （3） Freedom fi＇om pain, injury or disease; （4） Freedom to express normal behaviour; （5） Freedom from fear and distress.
文摘The rapid pace of development of GIS （geographical information system） has assisted in identification of conservation priority sites by delineating species distribution using models on habitat suitability. Gaur, Bos gaurus, is categorized as ＂Vulnerable＂ in the IUCN Red List of Threatened Species, 2009. The study has used ENFA （ecological niche factor analysis） to understand the distribution status of Gaur in TATR （Tadoba-Andhari Tiger Reserve）, Central India. TATR was sampled using stratified random sampling strategy. A total of 21 continuous variables were used, categorised under 4 environmental descriptors categories viz. habitat, anthropogenic, topographic and hydrological variables. All the variables were tested for the correlation and one of the variable among strongly correlated （r 〉 0.7） variables was discarded to avoid redundancy. A total of 14 variables were retained. The model resulted in marginality of 0.56 and specialization of 2.608. Presence of Gaur showed the positive association with canopy density classes （〈 30% ＆ 40-60%） and open forest. However, it was negatively associated with elevation, non-forest, riparian forest, scrub and teak forest. The study has delineated the areas where appropriate habitat conditions exist to sustain Gaur populations vital for planning strategies for conservation of this megaherbivore species in tropical forests.
文摘The objectives of the present study were to determine associations between these polymorphisms of PON1 gene and growth and carcass traits. For this purpose, genotyping was performed on males of 18 Angus, 23 Jinnan cattle, 20 Limousin, 28 Luxi cattle, 26 Qinchuan cattle, 29 Simmental, 29 Charolais. Traits of interest were analyzed using the RFLP-PCR and GLM procedure of SAS 9.1 and least square means of the genotypes were compared by the Tukey＇ s test in the association studies. Association of PONI/ EcoRV genotypes with body weight, average daily gain, rib eye area and tenderness were significant （P〈0.05）, and AG genotype was significant difference with others in average daily gain and tenderness （P〈0.05）, with AA genotype may have higher rib eye area （p〈0.05）. The association analysis of the PONI/Alu I polymorphism showed significant associations between genotypes and growth： body weight and carcass traits net meat weight and tenderness （P〈0.05）. AA genotype was considered more favorable than others in all growth and production traits. There were significant differences among breeds for all associated interests traits and difference between beef breeds and native breeds are obviously in all aspects. And these results may be useful for the breeding selection.
