Seventeen Sinorhizobium meliloti strains from seven provinces in China were used to screen highly effective strains for alfalfa cultivar in a greenhouse study and their symbiotic relationship and competitive ability w...Seventeen Sinorhizobium meliloti strains from seven provinces in China were used to screen highly effective strains for alfalfa cultivar in a greenhouse study and their symbiotic relationship and competitive ability were studied in the field. CCBAU30138 was the most effective strain, as evidenced by increase in dry weights. A field experiment showed that the inoculation of alfalfa with CCBAU30138 resulted in increases of 11.9% and 19.6% of dry matter production and crude protein production, respectively, in forage of monocultured plants. The total dry matter yields of alfalfa and tall fescue in binary culture were increased by 16.3% by inoculation of alfalfa with this strain. These results showed that S. rneliloti strain CCBAU30138 was an effective inoculant both in the greenhouse and in the field. The analysis of randomly amplified polymorphic DNA (RAPD) by polymerase chain reaction (PCR) from nodule extracts showed that the strain CCBAU30138 had high competitiveness in the field. It occupied 47.5% of nodules in alfalfa monoculture and 44.4% of nodules in alfalfa-tall fescue binary culture after 20 weeks of growth. In conclusion, a simple system to select highly effective and competitive symbiotic strains specific to alfalfa was established. Using this system, a s.train suitable for the alfalfa cultivar ‘Vector' grown in Wuqiao County of Hebei Province was obtained.展开更多
In this paper, a formulation for shakedown analysis of elastic-plastic offshore structures under cyclic wave loading is presented. In this formulation, a fast numerical solution method is used, suitable for the Finite...In this paper, a formulation for shakedown analysis of elastic-plastic offshore structures under cyclic wave loading is presented. In this formulation, a fast numerical solution method is used, suitable for the Finite Element Method (FEM) analysis of large offshore structures on which shear effects in addition to bending and axial effects are taken into account. The Morison equation is adopted for converting the velocity and acceleration terms into resultant forces and it is extended to consider arbitrary orientations of the structural members. The theoretical methods of the shakedown analysis are discussed in detail and the formulation is applied to an offshore structure to verify the concept employed and its analytical capabilities.展开更多
The aplastic anemia mice model was established in this study.BALB/c mice shall be treated with whole body irradiation with 60 Co-γradiation(5.5Gy,1.1Gy/min×5 min).then within 4 h,DBA/2 mice were injected lymphoc...The aplastic anemia mice model was established in this study.BALB/c mice shall be treated with whole body irradiation with 60 Co-γradiation(5.5Gy,1.1Gy/min×5 min).then within 4 h,DBA/2 mice were injected lymphocyte suspension 1×106 cells/mouse through caudal vein.Grouping of testing animals:Normal control mice were healthy C57BL/6 mice without AA modeling.AA control group mice were exposed to radiation and cell transfusion and had no treatment with either CSA or XDD.CSA group mice received daily lavage with 0.027g/kg(0.1ml/10g)of CSA whereas XDD group mice received daily lavage with 19.5g/kg of XDD.The experimental result indicated CSA and XDD lavage mice had significantly higher platelet count andΔΨm than AA mice(P<0.05).Levels of Cyt C,PS and Ca2+were significantly lower in CSA and XDD groups compare with AA group.More specifically,compared to CSA group,XDD group also had lower level ofΔΨm and higher level of Cyt C and Ca2+(both P<0.05).Both CSA and XDD treatment reduced Bak and Bax levels significantly compared to AA mice.However,XDD treatment still showed higher expressions than CSA(P<0.05).CSA and XDD treatment increased the levels of Pro-apoptotic protein expressions(caspase-8,caspase-3,caspase-9).XDD showed less potent effect than CSA in increasing these protein expressions(all P<0.05).Therefore,we hypothesized XDD was possible to increase platelet number and prevent its apoptosis in immune-induced AA via the mitochondrial pathway.展开更多
Polymyositis is an illness characterized by inflammation of the muscle(s) with uncertain etiological causes. Most of the physicians think that viral infection, disturbances of immune functions and/or pathological vasc...Polymyositis is an illness characterized by inflammation of the muscle(s) with uncertain etiological causes. Most of the physicians think that viral infection, disturbances of immune functions and/or pathological vascular changes might be responsible for the condition.展开更多
The interacting patterns of the luteolin and guanine have been investigated by using the density functional theory B3LYP method with 6-31+G* basis set. Eighteen stable structures for the luteolin-guanine complexes h...The interacting patterns of the luteolin and guanine have been investigated by using the density functional theory B3LYP method with 6-31+G* basis set. Eighteen stable structures for the luteolin-guanine complexes have been found respectively. The results indicate that the complexes are mainly stabilized by the hydrogen bonding interactions. Meanwhile, both the number and strength of hydrogen bond play important roles in determining the stability of the complexes which can form two or more hydrogen bonds. Theories of atoms in molecules and natural bond orbital have also been utilized to investigate the hydrogen bonds involved in all the systems. The interaction energies of all the complexes which were corrected by basis set superposition error are 6.04-56.94 kJ/mol. The calculation results indicate that there are strong hydrogen bonding interactions in the luteolin-guanine complexes. We compared the interaction between luteolin and four bases of DNA, and found luteolin-thymine was the strongest and luteolin-adenine was the weakest. The interaction between luteolin and DNA bases are all stronger than luteolin-water.展开更多
Eight flavonoid derivatives: rutin, quercetin-3-glucoside, quercetin, luteolin-7-glucoside, isorhmnetin-3-sulphate, kaempferol-3,7-diglucoside,'luteolin and kaempferol have been extracted and characterized from Nipa...Eight flavonoid derivatives: rutin, quercetin-3-glucoside, quercetin, luteolin-7-glucoside, isorhmnetin-3-sulphate, kaempferol-3,7-diglucoside,'luteolin and kaempferol have been extracted and characterized from Nipa palm. Mild extraction technique involving the use of HPLC-DAD-MS was used. The structures of the flavonoids were determined on the basis of mass spectroscopy. Separation of the crude extract by paper chromatography (PC) on forestall as solvent system gave one major yellowish brown spot which had Rf value of 3.9. The Rf value and maximum absorption from UV spectroscopy were the same as those of quercetin standard. The most prominent compound was quercetin followed by three others: kaempferol-3,7-diglucoside, luteolin-7-glucoside, and isorhmnetin-3 -sulphate.展开更多
基金Project supported by the National "Eleventh Five Years Plan" Key Project on Science and Technology (No. 2006BAD02A15)the National Natural Science Foundation of China (No. 30671222).
文摘Seventeen Sinorhizobium meliloti strains from seven provinces in China were used to screen highly effective strains for alfalfa cultivar in a greenhouse study and their symbiotic relationship and competitive ability were studied in the field. CCBAU30138 was the most effective strain, as evidenced by increase in dry weights. A field experiment showed that the inoculation of alfalfa with CCBAU30138 resulted in increases of 11.9% and 19.6% of dry matter production and crude protein production, respectively, in forage of monocultured plants. The total dry matter yields of alfalfa and tall fescue in binary culture were increased by 16.3% by inoculation of alfalfa with this strain. These results showed that S. rneliloti strain CCBAU30138 was an effective inoculant both in the greenhouse and in the field. The analysis of randomly amplified polymorphic DNA (RAPD) by polymerase chain reaction (PCR) from nodule extracts showed that the strain CCBAU30138 had high competitiveness in the field. It occupied 47.5% of nodules in alfalfa monoculture and 44.4% of nodules in alfalfa-tall fescue binary culture after 20 weeks of growth. In conclusion, a simple system to select highly effective and competitive symbiotic strains specific to alfalfa was established. Using this system, a s.train suitable for the alfalfa cultivar ‘Vector' grown in Wuqiao County of Hebei Province was obtained.
文摘In this paper, a formulation for shakedown analysis of elastic-plastic offshore structures under cyclic wave loading is presented. In this formulation, a fast numerical solution method is used, suitable for the Finite Element Method (FEM) analysis of large offshore structures on which shear effects in addition to bending and axial effects are taken into account. The Morison equation is adopted for converting the velocity and acceleration terms into resultant forces and it is extended to consider arbitrary orientations of the structural members. The theoretical methods of the shakedown analysis are discussed in detail and the formulation is applied to an offshore structure to verify the concept employed and its analytical capabilities.
文摘The aplastic anemia mice model was established in this study.BALB/c mice shall be treated with whole body irradiation with 60 Co-γradiation(5.5Gy,1.1Gy/min×5 min).then within 4 h,DBA/2 mice were injected lymphocyte suspension 1×106 cells/mouse through caudal vein.Grouping of testing animals:Normal control mice were healthy C57BL/6 mice without AA modeling.AA control group mice were exposed to radiation and cell transfusion and had no treatment with either CSA or XDD.CSA group mice received daily lavage with 0.027g/kg(0.1ml/10g)of CSA whereas XDD group mice received daily lavage with 19.5g/kg of XDD.The experimental result indicated CSA and XDD lavage mice had significantly higher platelet count andΔΨm than AA mice(P<0.05).Levels of Cyt C,PS and Ca2+were significantly lower in CSA and XDD groups compare with AA group.More specifically,compared to CSA group,XDD group also had lower level ofΔΨm and higher level of Cyt C and Ca2+(both P<0.05).Both CSA and XDD treatment reduced Bak and Bax levels significantly compared to AA mice.However,XDD treatment still showed higher expressions than CSA(P<0.05).CSA and XDD treatment increased the levels of Pro-apoptotic protein expressions(caspase-8,caspase-3,caspase-9).XDD showed less potent effect than CSA in increasing these protein expressions(all P<0.05).Therefore,we hypothesized XDD was possible to increase platelet number and prevent its apoptosis in immune-induced AA via the mitochondrial pathway.
文摘Polymyositis is an illness characterized by inflammation of the muscle(s) with uncertain etiological causes. Most of the physicians think that viral infection, disturbances of immune functions and/or pathological vascular changes might be responsible for the condition.
文摘The interacting patterns of the luteolin and guanine have been investigated by using the density functional theory B3LYP method with 6-31+G* basis set. Eighteen stable structures for the luteolin-guanine complexes have been found respectively. The results indicate that the complexes are mainly stabilized by the hydrogen bonding interactions. Meanwhile, both the number and strength of hydrogen bond play important roles in determining the stability of the complexes which can form two or more hydrogen bonds. Theories of atoms in molecules and natural bond orbital have also been utilized to investigate the hydrogen bonds involved in all the systems. The interaction energies of all the complexes which were corrected by basis set superposition error are 6.04-56.94 kJ/mol. The calculation results indicate that there are strong hydrogen bonding interactions in the luteolin-guanine complexes. We compared the interaction between luteolin and four bases of DNA, and found luteolin-thymine was the strongest and luteolin-adenine was the weakest. The interaction between luteolin and DNA bases are all stronger than luteolin-water.
文摘Eight flavonoid derivatives: rutin, quercetin-3-glucoside, quercetin, luteolin-7-glucoside, isorhmnetin-3-sulphate, kaempferol-3,7-diglucoside,'luteolin and kaempferol have been extracted and characterized from Nipa palm. Mild extraction technique involving the use of HPLC-DAD-MS was used. The structures of the flavonoids were determined on the basis of mass spectroscopy. Separation of the crude extract by paper chromatography (PC) on forestall as solvent system gave one major yellowish brown spot which had Rf value of 3.9. The Rf value and maximum absorption from UV spectroscopy were the same as those of quercetin standard. The most prominent compound was quercetin followed by three others: kaempferol-3,7-diglucoside, luteolin-7-glucoside, and isorhmnetin-3 -sulphate.