是“熊猫”还是“猫熊”

第5期“看谁想得妙”中，第17条妙想这样写道：“这是一只猫熊，猫熊是从来不冬眠的”。这里的“猫熊”应该是“熊猫”吧？

Age Differences in Behavioral Responses of Male Giant Pandas to Chemosensory Stimulation

Chemical communication plays an important role in survival and reproductive success in mammalian species. In the present study, we examined the ontogenetic pattern of behavioral responses of male giant pandas （Ailuropoda melanoleuca ） to urine odors of conspecific individuals. Our data showed that exposure to the urine of adult females induced a significant increase in sniffing and environmental sniffing/licking behaviors, but a decrease in biting behavior, in males. Males of different ages displayed specific behaviors to female urine odors. Adult males spent more time licking than juvenile and sub-adult males. Further, sub-adult and adult males displayed high levels of environmental sniffing/licking, which was absent in the juvenile males. Juvenile males displayed scent rubbing behavior significantly more frequently than sub-adult and adult males, and also spent more time showing biting behavior than sub-adult males. Finally, juvenile and sub-aduh males showed no difference in response to female and male urine odors. Together, these data suggest that chemosensory cues from conspecific urines induce age-specific responses in male giant pandas.

PHYLOGENETIC RELATIONSHIPS AMONG GIANT PANDA AND RELATED SPECIES BASED ON RESTRICTION SITE VARIATIONS IN rDNA SPACERS

In this study, non radioactive Digoxigenin labeled ribosomal DNA(rDNA) probes were used for Southern blotting analysis to study the molecular phylogeny of the giant panda and related species. Restriction maps in the regions of rDNA spacers were compared between giant panda( Ailuropoda melanoleuca ), lesser panda( Ailurus fulgens ), Asiatic black bear( Selenarctos thibetanus ), sun bear( Helarctos malayanus ), raccoon( Procyon lotor ) and lynx( Felis lynx ). Phylogenetic trees for these species were constructed using maximum likelihood and parsimony method. The results show that in respect to rDNA RFLPs, the giant panda is more closely related to bear than to lesser panda; while the lesser panda is slightly related to the raccoon.

Serological survey on canine coronavirus antibodies in giant pandas by virus neutralization test

In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection.

Efficiency of Artificial Insemination in Giant Pandas at the Wolong Breeding Center

The goal of the giant panda ( Ailuropoda melanoleuca ) breeding program is to develop a self sustaining,genetically diverse population.Due to the common problems about sexual incompatibility and a limited number of captive born males that breed naturally,artificial insemination (AI) has become a critical genetic management tool.It is common practice,however,to combine natural mating and AI using semen from non breeding males.From 1998 to 2000 at the Wolong breeding facility,12 of 18 (66.7%) females produced 20 cubs following combined natural mating and AI.The objective of this study was to determine the efficiency of AI without natural breeding.In 1998 and 2000,seven females were anesthetized for transcervical AI on two consecutive days.Ejaculates from six males were collected by electroejaculation,diluted in an egg yolk diluent containing 0% or 4% glycerol and used either fresh or following cold storage at 4℃ (for 24 or 48 h) or cryopreservation using the pellet freezing method.Mean (±SEM) ejaculate traits in six male sperm donors were:ejaculate volume,3.3±0.5 ml;sperm concentration,1,429.8±235.4×10 6/ml;sperm motility,81.7±2.1%;progression (0～5,5=best),3.1±0.1;and normal sperm,79.3±9.2%.For AI (n = 14) in seven females,mean inseminate traits were:spermic volume inseminated,2.4±0.3 ml;sperm motility,73.5±2.9%;progression,2.5±0.1;and total motile sperm inseminated/AI,684.2±118.2×10 6.Four of seven (57.1%) females became pregnant and produced five cubs of which four survived.Mean gestation and litter size was 131.5±9.7 days and 1.3±0.3 cubs/litter,respectively.These results indicate that the efficiency of AI is sufficient for recovering valuable genes from non breeding individuals to enhance genetic diversity in the ex situ population of giant pandas.

A Remote Sensing-based Analysis on the Impact of Wenchuan Earthquake on the Core Value of World Nature Heritage Sichuan Giant Panda Sanctuary

In this study,we used remotely sensed data,GIS modeling,and statistical methods to evaluate the damage caused by the Wenchuan Earthquake (May 12,2008) to the giant panda (Ailuropoda melanoleuca) habitat in the World Nature Heritage Sichuan Giant Panda Sanctuary (WHSGPS) in China.A landscape ecological analysis found increases of landscape heterogeneity,complexity,and fragmentation in the giant panda habitat after the earthquake.A terrain analysis found that slope and elevation are directly associated with the distribution of the damaged areas.As slope and elevation increase,the size of the damaged area keeps increase until to a peak,and then starts to drop.The total area of the damaged vegetation in our study area is 114.26 km 2,accounting for 3.78% of the study area;30.46% of that 114.26 km 2 is located in the Core Zone of WHSGPS.There are 18.57km 2 of the damaged vegetation located in the identified suitable giant panda habitat,accounting for 1.75% of the total area of suitable giant panda habitats in the study area.Based on these findings,we conclude that the Wenchuan Earthquake does not have significant impact on the WHSGPS as a whole.

Evaluating Conservation Effectiveness of Nature Reserves Established for Surrogate Species:Case of a Giant Panda Nature Reserve in Qinling Mountains,China

Many nature reserves are established to protect the habitat needs of particular endangered species of interest but their effectiveness for protecting other species is questionable.In this study,this effectiveness was evaluated in a nature reserve network located in the Qinling Mountains,Shaanxi Province,China.The network of reserves was established mainly for the conservation of the giant panda,a species considered as a surrogate for the conservation of many other endangered species in the region.The habitat suitability of nine protected species,including the giant panda,was modeled by using Maximum Entropy(MAXENT)and their spatial congruence was analyzed.Habitat suitability of these species was also overlapped with nature reserve boundaries and their management zones(i.e.,core,buffer and experimental zones).Results show that in general the habitat of the giant panda constitutes a reasonable surrogate of the habitat of other protected species,and giant panda reserves protect a relatively high proportion of the habitat of other protected species.Therefore,giant panda habitat conservation also allows the conservation of the habitat of other protected species in the region.However,a large area of suitable habitat was excluded from the nature reserve network.In addition,four species exhibited a low proportion of highly suitable habitat inside the core zones of nature reserves.It suggests that a high proportion of suitable habitat of protected species not targeted for conservation is located in the experimental and buffer zones,thus,is being affected by human activities.To increase their conservation effectiveness,nature reserves and their management zones need to be re-examined in order to include suitable habitat of more endangered species.The procedures described in this study can be easily implemented for the conservation of many endangered species not only in China but in many other parts of the world.

Impacts of the Wenchuan Earthquake on the Giant Panda Nature Reserves in China

The Wenchuan Earthquake that occurred in May of 2008 caused damages to large areas of Sichuan,Gansu,and Shaanxi provinces in China.Reports from local governments and related management agencies show that the giant panda nature reserves in the earthquake-hit areas were heavily damaged.Our estimates in this paper of the impacts of the earthquake on the giant panda in the earthquake-hit areas were made based on the interpretation of remote sensing images and information collected by field survey.A rapid assessment method was designed to estimate the damages of the earthquake on giant panda habitats.By using visual interpretation methods,we decoded the remote sensing images of the disaster area in the 49 giant panda nature reserves.Research results showed that the Wenchuan Earthquake and the succeeding secondary geological disasters caused great damages to the giant panda nature reserves and disturbed the normal life of the giant pandas there (e.g.,landscape fragmentation increased significantly).Undoubtedly,the life of the giant pandas there was affected.However,although the earthquake caused certain impacts on the giant pandas,it did not really threat their survival.Even so,we still strongly advocate for protection of the giant pandas,and have prioritized a couple of measures to be taken to restore the giant panda nature reserves in the earthquake-hit areas.

An approach on the survivorship of giant panda in wild

The endangered giant panda (Ailuropoda melanoleuca) is endemic to th e mountains of Sichuan, Gansu and Shaanxi Provinces in China. The species had nu mbered over 2000 animals in early 1970s, but declined to no more than 1,000 anim als fragmented into perhaps 32 subpopulations in late 1990s as the result of num erous detrimental forces such as habitat shrinking, poaching and bamboo flowerin g. The mass flowering and followed die-off of bamboo played key roles in the dec lination of panda population in the past three decades. It trigged the starvatio n and following high mortality of giant pandas in 1970s over Min Mountains and i n 1980s across Qionglai Mountains in Sichuan Province. The situation of survivor ship is made worse by the fact that the panda habitat is fragmented into many sm all 'islands', each containing only a few pandas. Such small, isolated panda pop ulations are rendered even more vulnerable to extinction form threats such as ha bitat degradation, natural disaster, disease, and the deleterious effects of inb reeding. So restoring the panda habitat and reintroduction pandas to their histo rical habitat might be an important issue for protecting the giant panda in wild .

SEASONAL CHANGES OF PROGESTE RONE AND TESTOSTERONE CONTENTS IN HAIR OF GIANT PANDAS

Seasonal changes of progesterone content (PSC) in hair of three female giant pandas and testosterone content (TSC) in hair of two male giant pandas were tested. It was found that the two hormones could be detected by radioimmunoassay in all the hair samples scissored from the female and male giant pandas respectively. During March to early June, PSC in two non-pregnent giant pandas (±SD = 13.40 ± 10.06 and10.60±8.88 ng / g hair respectively) were higher than those in non-breeding season (3.07 ± 1.07 and 3.20 ±1.15 ng / g, P< 0.01). PSC in a 18--year--old female giant panda remained at low levels (2.72 ± 1.49 ng / g) during March to December. In a twin--beargiant panda, PSC (6.77± 3.66 ng / g) appeared higher than that.in non--pregnant giantpandas in non--breeding season. Around February tO the end of May, TSC in two male giant pandas (1.89± 1.71 and 1.82 ± 1.04 ng / g respectively) were also higher than that in non-breeding season (0.98 ± 0.57 and 0.75 ± 0.39 ng / g, P< 0.01). The findings from the study implied that giant panda's hair is possible to be used as a specimen tO carryout steroids research in the endangered species.

Panda Habitat Added to World Heritage List

“Bamboo has blossomed. Held in her mother’s arms, Mimi is counting the number of stars in the sky. How beautiful the stars are and where is my breakfast tomorrow...

The Embodiment of American Elements In Oriental Story of Kung Fu Panda

This thesis aims at the study of the American elements in oriental story Kung Fu Panda which was made by DreamWorks of Hollywood. Firstly, the thesis shows what the basic classic American characteristics are and the definition of American Dream, as well as some basic study on Hollywood＇s operation of movies. Then, it analyzes the above traits in Kung Fu Panda, the embodiment of American elements in Kung Fu Panda and the recipe for Hollywood＇s manufacturing success. At last, based on the analyses which have been pointed out, this thesis tries to arrive at the conclusion that although with an oriental-story setting, Kung Fu Panda is still American-styled and at the same time, this thesis gives some suggestive strategies that can help Chinese movie industry step out of the awkward situation.

On the Chinese Elements in Hollywood Movies from Kung fu Panda

With the development of globalization, movie, as the important media for broadcasting and exchanging cultures, promotes the international cultural communications among countries. Kung fu Panda, a blockbuster by Dream works, sets off a new upsurge of the Chinese style. It not only gives a chance for the audiences in western countries to enjoy the traditional Chinese culture, but also for the Chinese to enjoy this visual feast. This article will illustrate the implicit and explicit Chinese elements in Kung fu Panda, in hope of finding out the extensive use of Chinese elements in today＇s Hollywood movies. According to the collection of media data concluded, the film production team undertook detailed investigation and studies in the traditional Chinese culture so that today＇s Hollywood movies can successfully interpret Chinese elements. Thus, the international cultural communications are found hiding behind the widely used of Chinese elements. With the analysis of this article, Chinese reader can know further about how important the diffusion of Chinese traditional culture is. Thereby, it can remind Chinese people of the responsibility to carry on and broadcast the splendid traditional Chinese culture.

A Graph-theoretic Analysis of Stressors Confronting Giant Panda Habitat across Minshan Mountain Region,China

岷山地区位于四川盆地西北部,是我国生物多样性保护的优先地区,还是保护大熊猫的关键区域。本文以社会-经济-自然复合生态系统为基础,运用图论分析法研究了岷山地区影响大熊猫生境的人类活动方式,以及这些影响因子相互的关系。研究表明:影响大熊猫生境的人类活动的66对组合中,47.0%的因子间存在直接的联系,其中89.2%的影响具有增大效应。这说明人类活动对大熊猫生境的影响具有协同增大效应,并占主导地位。旅游景点开发、公路建设、采矿、高压电线、农业开发、放牧、薪柴采集这些影响因子存在强连通性,它们会将其他人类活动对大熊猫生境的影响放大,加剧对大熊猫生境的不利影响,表明它们是影响岷山地区大熊猫生境的主导人类活动。要想有效地保护岷山地区的大熊猫生境,就必须对这些主导人类活动进行有效的控制。本研究还表明图论分析法有助于明确影响熊猫生境的主导和关键因子,它能为制定有效的大熊猫保护策略提供科学依据。

Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu, China

A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1T grew optimally at pH 7.0-8.0, at 28-30 ℃ and in the presence of NaCI concentrations from 0.0% to 0.5%. 16S rRNA gene sequence analysis indicated that strain 25-1m belongs to the genus Chryseobacterium within the family Flavo- bacteriaceae and is related most closely to C. camis G81m （96.4% similarity）, C. lathyri RBA2-6T （95.8% similarity）, and C. zeae JM1085T （95.8% similarity）. Its genomic DNA G＋C molar composition was 36.2%. The major cellular fatty acids were iso-C15：0 （44.0%）, iso-C17：0 3OH （19.8%） and C16：1 ω7c/16：1 ω6c （12.7%）. The only isoprenoid quinone was menaquinone 6 （MK-6）. The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA-DNA relatedness between strain 25-1m and C. lathyri RBA2-6T was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1T is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1T （ccmoc AB2015133T=DSM 100396T）.

Periodical rocking long period gratings in PANDA fibers for high temperature and refractive index sensing

We report periodical rocking long period gratings(PR-LPGs) in PANDA fibers fabricated with CO_2 laser. The PR-LPGs achieve very high coupling efficiency of 19 dB with 12 periods and a 3.5° twist angle in just one scanning cycle, which is much more effective than the conventional CO_2 laser fabrication technique. This type of LPGs exhibits polarization-selective resonance dips which demonstrate different sensitivities to environmental parameters. The high temperature and external refractive index sensitivities are measured simultaneously, so it can be used as a wavelength-selective polarization filter and sensor.

Panda X-III： Searching for neutrinoless double beta decay with high pressure ^(136)Xe gas time projection chambers

Searching for the neutrinoless double beta decay(NLDBD)is now regarded as the topmost promising technique to explore the nature of neutrinos after the discovery of neutrino masses in oscillation experiments.Panda X-III(particle and astrophysical xenon experiment III)will search for the NLDBD of136Xe at the China Jin Ping Underground Laboratory(CJPL).In the first phase of the experiment,a high pressure gas Time Projection Chamber(TPC)will contain 200 kg,90%136Xe enriched gas operated at10 bar.Fine pitch micro-pattern gas detector(Microbulk Micromegas)will be used at both ends of the TPC for the charge readout with a cathode in the middle.Charge signals can be used to reconstruct the electron tracks of the NLDBD events and provide good energy and spatial resolution.The detector will be immersed in a large water tank to ensure～5 m of water shielding in all directions.The second phase,a ton-scale experiment,will consist of five TPCs in the same water tank,with improved energy resolution and better control over backgrounds.

Microsatellite variability reveals high genetic diversity and low genetic differentiation in a critical giant panda population

Understanding present patterns of genetic diversity is critical in order to design effective conservation and manage- ment strategies for endangered species. Tangjiahe Nature Reserve （NR） is one of the most important national reserves for giant pandas Ailuropoda melanoleuca in China. Previous studies have shown that giant pandas in Tangjiahe NR may be threatened by population decline and fragmentation. Here we used 10 microsatellite DNA markers to assess the genetic variability in the Tang- jiahe population. The results indicate a low level of genetic differentiation between the Hongshihe and Motianling subpopulations in the reserve. Assignment tests using the Bayesian clustering method in STRUCTURE identified one genetic cluster from 42 in- dividuals of the two subpopulations. All individuals from the same subpopulation were assigned to one cluster. This indicates high gene flow between subpopulations. F statistic analyses revealed a low Fzs-value of 0.024 in the total population and implies a randomly mating population in Tangjiahe NR. Additionally, our data show a high level of genetic diversity for the Tangjiahe population. Mean allele number （A）, Allelic richness （AR） and mean expected heterozygosity （HE） for the Tangjiahe population was 5.9, 5.173 and 0.703, respectively. This wild giant panda population can be restored through concerted effort

Chromosome-scale genomes provide new insights into subspecies divergence and evolutionary characteristics of the giant panda

Extant giant pandas are divided into Sichuan and Qinling subspecies.The giant panda has many speciesspecific characteristics,including comparatively small organs for body size,small genitalia of male individuals,and low reproduction.Here,we report the most contiguous,high-quality chromosomelevel genomes of two extant giant panda subspecies to date,with the first genome assembly of the Qinling subspecies.Compared with the previously assembled giant panda genomes based on short reads,our two assembled genomes increased contiguity over 200-fold at the contig level.Additional sequencing of 25 individuals dated the divergence of the Sichuan and Qinling subspecies into two distinct clusters from 10,000 to 12,000 years ago.Comparative genomic analyses identified the loss of regulatory elements in the dachshund family transcription factor 2(DACH2)gene and specific changes in the synaptotagmin 6(SYT6)gene,which may be responsible for the reduced fertility of the giant panda.Positive selection analysis between the two subspecies indicated that the reproduction-associated IQ motif containing D(IQCD)gene may at least partly explain the different reproduction rates of the two subspecies.Furthermore,several genes in the Hippo pathway exhibited signs of rapid evolution with giant panda-specific variants and divergent regulatory elements,which may contribute to the reduced inner organ sizes of the giant panda.

Balancing selection and recombination drive genetic variation at MHC class I genes in the giant panda

Major histocompatibility complex （MHC） is a family of highly polymorphic genes activating adaptive immunity in vertebrates. However, the underlying mecha- nism of MHC evolution is still not fully understood. In this study, we investigated genetic variation of three classical MHC class I genes in the giant panda （Ailuropoda mela- noleuca） and tested for selection effect and recombination event across exonic and intronic sequences to understand maintenance mechanism of polymorphism at Aime-MHC class I genes. In total, we isolated 21 MHC class I haplotypes （exon 2-intron 2-exon 3） from 46 captive giant pandas, of which eight were for Aime-C, seven for Aime-I and six for Aime-L; however, we only identified six unique sequences from these haplotypes. The subsequent maximum-likeli- hood and Chi-square analyses both detected evidence of recombination acting on the 21 haplotypes. These results indicate that the giant panda still retains a relatively high adaptive variation at Aime-MHC-I genes, and that the intronic segments have been homogenized along evolu- tionary time by recombination and subsequent genetic drift.We calculated nucleotide substitution rates of the antigen- binding regions （exons 2 and 3） and the noncoding intron 2, and found two pieces of evidence supporting the presence of balancing selection in the giant panda： an excess of nonsynonymous over synonymous substitutions at the antigen-binding sites, and an obviously higher synonymous substitutions in the exons than nucleotide substitutions in the intron. Thus, this study reveals that balancing selection and recombination together shape the diversity pattern at Aime- MHC-I loci of the giant panda.