[ Objective] The aim was to study the protein polymorphism in the blood of Tibetan Mastiff, and provide some theoretical basis for resource protection and reasonable development and utilization of Tibetan Mastiff vari...[ Objective] The aim was to study the protein polymorphism in the blood of Tibetan Mastiff, and provide some theoretical basis for resource protection and reasonable development and utilization of Tibetan Mastiff varieties. [ Method] A total of 103 blood samples were taken from four populations of Hequ Tibetan Mastiff, Qinhai Tibetan Mastiff, Tibetan Spaniel and native dogs of Qinghai. Seven blood protein Iocus（Tf, Po, Sα2, Hb, AIb, Pr and Amy）were investigated by using vertical polyacrylamide gel electrophoresis with discontinuous buffer system. Then the genetic variation during different populations was analyzed. [ Result] Genetic variations were observed in Tf, Sα2 and Po in four populations, others were not polymorphic. There were three alleles at the locus of Tf and Po, two alleles at the loci of Sα2. Effective number of alleles and Nei＇s average expected heterozygosity were 1. 532 4 and 0.230 3 relatively, all higher in Tibetan Mastiff than other populations. [ Conclusion] Protein locus in blood of Tibetan Mastiff existed in genetic variation.展开更多
[Objective] This study aimed to investigate the effects of cryopreservation on the quality and ultrastructure of Tibetan Mastiff sperm. [Method] The effects of cryopreservation on the quality of Tibetan Mastiff sperm ...[Objective] This study aimed to investigate the effects of cryopreservation on the quality and ultrastructure of Tibetan Mastiff sperm. [Method] The effects of cryopreservation on the quality of Tibetan Mastiff sperm were evaluated via motility, membrane integrity rate and acrosome intact rate. On that basis, the effects of cryopreservation on ultrastructure of sperm were observed under SEM and TEM. [Result] In Experiment 1, EG gave the best results not only in post-thaw motility rate （36.3%）, but also in low membrane integrity rate （38.0%） and acrosome intact rate （42.0% ）, but there was no significant difference between EG group and Glycerol group （P0.05）. In Experiment 2, the 5 cm freezing height obtained the best freezing-thawing results, but there was no significant difference between 2 and 5 cm height （P 0.05）, besides in acrosome intact rate. In Experiment 3, SDS and Vc added separately or together into extenders could improve freezing-thawing results, but there was not obvious difference between SDS group and Vc group （P0.05）, besides the lower motility of Vc group （P0.05）. Addition of SDS and Vc obtained the best results in post-thaw motility rate （44.1%）, and also in membrane integrity rate （48.0%） and acrosome intact rate （48.2%）. The ultrastructure of frozen-thawed sperm was also evaluated under SEM and TEM, results showed that cryopreservation caused various degrees of damage to Tibetan Mastiff sperm, more serious damages were observed in the acrosome such as swelling, vesiculation and even disappearance. [Conclusion] This study confirms that EG, horizontal height of 0.25 ml straw above LN 2 surface and additives SDS and Vc together can improve freezing effect. However, cryopreservation has certain damage to ultrastructure of sperm.展开更多
This paper gave the standards to evaluate Hequ Tibetan Mastiff on body type, appearance, body measures and temperament. A nucleus group of breeding consisted of high-grade individual according this standards, and it w...This paper gave the standards to evaluate Hequ Tibetan Mastiff on body type, appearance, body measures and temperament. A nucleus group of breeding consisted of high-grade individual according this standards, and it was for enhancing the proceeding of selecting and breeding of Hequ Tibetan Mastiff. By this way, after working on three generations it had been gained that effective population size of the nucleus group of breeding was 96, increasing numbers of inbreeding coefficient was 0.005, male and female body height were 72.1 cm and 70.2 cm, their cannon circumference were 16.3 cm and 15.2 cm, respectively.展开更多
基金Supported by Foundation of Gansu Technology Committee (GKC-97-27-5)Youth Foundation of Tianshui Normal University (X4-25)~~
文摘[ Objective] The aim was to study the protein polymorphism in the blood of Tibetan Mastiff, and provide some theoretical basis for resource protection and reasonable development and utilization of Tibetan Mastiff varieties. [ Method] A total of 103 blood samples were taken from four populations of Hequ Tibetan Mastiff, Qinhai Tibetan Mastiff, Tibetan Spaniel and native dogs of Qinghai. Seven blood protein Iocus（Tf, Po, Sα2, Hb, AIb, Pr and Amy）were investigated by using vertical polyacrylamide gel electrophoresis with discontinuous buffer system. Then the genetic variation during different populations was analyzed. [ Result] Genetic variations were observed in Tf, Sα2 and Po in four populations, others were not polymorphic. There were three alleles at the locus of Tf and Po, two alleles at the loci of Sα2. Effective number of alleles and Nei＇s average expected heterozygosity were 1. 532 4 and 0.230 3 relatively, all higher in Tibetan Mastiff than other populations. [ Conclusion] Protein locus in blood of Tibetan Mastiff existed in genetic variation.
基金Natural Science Foundation of Jiangsu Province (BK2008589)Qing Lan Project of Jiangsu ProvinceCooperation Project of Agricultural Production, Study and Research in Wuxi City~~
文摘[Objective] This study aimed to investigate the effects of cryopreservation on the quality and ultrastructure of Tibetan Mastiff sperm. [Method] The effects of cryopreservation on the quality of Tibetan Mastiff sperm were evaluated via motility, membrane integrity rate and acrosome intact rate. On that basis, the effects of cryopreservation on ultrastructure of sperm were observed under SEM and TEM. [Result] In Experiment 1, EG gave the best results not only in post-thaw motility rate （36.3%）, but also in low membrane integrity rate （38.0%） and acrosome intact rate （42.0% ）, but there was no significant difference between EG group and Glycerol group （P0.05）. In Experiment 2, the 5 cm freezing height obtained the best freezing-thawing results, but there was no significant difference between 2 and 5 cm height （P 0.05）, besides in acrosome intact rate. In Experiment 3, SDS and Vc added separately or together into extenders could improve freezing-thawing results, but there was not obvious difference between SDS group and Vc group （P0.05）, besides the lower motility of Vc group （P0.05）. Addition of SDS and Vc obtained the best results in post-thaw motility rate （44.1%）, and also in membrane integrity rate （48.0%） and acrosome intact rate （48.2%）. The ultrastructure of frozen-thawed sperm was also evaluated under SEM and TEM, results showed that cryopreservation caused various degrees of damage to Tibetan Mastiff sperm, more serious damages were observed in the acrosome such as swelling, vesiculation and even disappearance. [Conclusion] This study confirms that EG, horizontal height of 0.25 ml straw above LN 2 surface and additives SDS and Vc together can improve freezing effect. However, cryopreservation has certain damage to ultrastructure of sperm.
文摘This paper gave the standards to evaluate Hequ Tibetan Mastiff on body type, appearance, body measures and temperament. A nucleus group of breeding consisted of high-grade individual according this standards, and it was for enhancing the proceeding of selecting and breeding of Hequ Tibetan Mastiff. By this way, after working on three generations it had been gained that effective population size of the nucleus group of breeding was 96, increasing numbers of inbreeding coefficient was 0.005, male and female body height were 72.1 cm and 70.2 cm, their cannon circumference were 16.3 cm and 15.2 cm, respectively.
