AIM： To examine how High-mobility group box I （HMGB1） regulates hepatocyte apoptosis and, furthermore, to determine whether glycyrrhizin （GL）, a known HMGB1 inhibitor, prevents HMGBl-induced hepatocyte apoptosis.
Plants synthesize the osmoprotectant glycine betaine (GB) via choline→betaine aldehyde→glycine be- taine[1]. Two enzymes are involved in the pathway choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BA...Plants synthesize the osmoprotectant glycine betaine (GB) via choline→betaine aldehyde→glycine be- taine[1]. Two enzymes are involved in the pathway choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH). A full length CMO cDNA (1,643bp) was cloned from Amaranthus tricolor. The open reading frame encoded a 442-amino acid polypeptide, which showed 69% identity with CMOs in Spina- cia oleracea L. and Beta vulgaris L. DNA gel blot analysis indicated the presence of one copy of CMO gene in the A. tricolor genome. The expressions of CMO and BADH proteins in A.tricolor leaves significantly increased under salinization, drought and heat stress (42℃), as determined by immunoblot analysis, but did not respond to cold stress (4℃), or exogenous ABA application. The increase of GB content in leaves was parallel to CMO and BADH contents.展开更多
Bagasse fly ash was converted into an inexpensive adsorbent andutilized for the removal of β- naphthalenesulfonic acid in dilutesolution. The effect of pH, temperature, adsorbent concentration, andco-existed acids on...Bagasse fly ash was converted into an inexpensive adsorbent andutilized for the removal of β- naphthalenesulfonic acid in dilutesolution. The effect of pH, temperature, adsorbent concentration, andco-existed acids on the removal of β-naphthalenesulfonic acid wasexamined. The adsorption data have been correlated with both Langmuirand Freundlich adsorption models. Thermodynamic parameters obtainedindicate the feasibility of the process, and kinetic studies providedthe necessary mechanistic information of the removal process.展开更多
A six-week growth trial was conducted to compare the effects of different feeding strate- gies of dietary immunostimulants on the growth and immunity of white shrimp Litopenaeus vannamei （4.70 ±0.20g）. Shrimps ...A six-week growth trial was conducted to compare the effects of different feeding strate- gies of dietary immunostimulants on the growth and immunity of white shrimp Litopenaeus vannamei （4.70 ±0.20g）. Shrimps were fed with diet containing glycyrrhizin continuously, containing β -glucan continuously, discontinuously （seven days with diet containing β -gluseven days with diet without -glucan; two days with diet containing β-glucan following five days with diet without -glucan）,展开更多
Sugar beet tops including leaves and crown harvested and chopped into 3-6 cm, mixed with sugar cane pulp and sugar cane molasses （about 5% of the total mass） to provide a mixture contained 30-35 percent dry mater. A...Sugar beet tops including leaves and crown harvested and chopped into 3-6 cm, mixed with sugar cane pulp and sugar cane molasses （about 5% of the total mass） to provide a mixture contained 30-35 percent dry mater. After two months of ensiling, the silage was evaluated and used in the ration of lactating buffaloes. A Latin square experiment in a changeover design, with three periods of time, each with 20 days for adaptation and 10 days for recording data, was conducted in which 15 multiparous lactating buffloes were used in three similar groups, each with five animals. The prepared silage was included in the diet of lactating buffaloes where a control diet （A） was compared with treatment diets contained 25% （B） and 50% （C） silage （DM basis） respectively. Milk yield was recorded, sampled and analysed for fat, protein, solid not-fat and total solids-content. The maximum daily rough milk yield per animal was obtained on diet C while minimum on diet A （P 〈 0.05）. Similar trends were observed for 4% fat corrected milk, and 3.5% fat corrected milk that were significantly different between the treatments （P 〈 0.05）. The percentage of crude fat and daily fat yield per animal were significantly （P 〈 0.05） higher in treatment C comparing to A and B. An increasingly trend of milk and fat yield was observed when the animals received silage contained diets but statistically it was significant only for the diets contained 50% silage. In addition, the fat percentage was significantly （P 〈 0.05） increased when the buffaloes received diet containing 50% silage. The highest （P 〈 0.05） amount of protein and total solid yield obtained, when the animals received high （50%） silage diet.展开更多
Objective： To investigate the neuropro- tective effects of glycyrrhizin （Gly） as well as its effect on expression of high-mobility group box 1 （HMGB1） in rats after traumatic brain injury （TBI）. Methods： Male...Objective： To investigate the neuropro- tective effects of glycyrrhizin （Gly） as well as its effect on expression of high-mobility group box 1 （HMGB1） in rats after traumatic brain injury （TBI）. Methods： Male Sprague-Dawley rats were randomly divided into three groups： sham group, TBI group, and TBI＋Gly group （n=36 per group）. Rat TBI model was made by using the modified Feeney＇s method. In TBI＋Gly group, Gly was administered intravenously at a dosage of l0 mg/kg 30 min after TBI. At 24 h after TBI, motor function and brain water content were evaluated. Meanwhile, HMGB 1/HMGB 1 receptors including toll-like receptor 4 （TLR4） and receptor for advanced glycation end products （RAGE）/nuclear fac- tor-κ B（NF- κ B） signaling pathway and inflammatory cytokines in the injured brain tissues were detected using quantitative real-time polymerase chain reaction, western blot, electrophoretic mobility shift assay and enzyme-linked immunosorbent assay. Furthermore, HMGB l, RAGE and TLR4 immunohistochemistry and apoptosis were analyzed. Results： Beam walking performance impairment and brain edema were significantly reduced in TBI＋Gly group compared with TBI group; meanwhile, the over-expressions of HMGB 1PHMGB 1 receptors （TLR4 and RAGE）/NF- κB DNA-binding activity and inflammatory cytokines were inhibited. The percentages of HMGB 1, RAGE and TLR4- positive cells and apoptotic cells were respectively 58.37%±5.06%, 54.15%±4.65%, 65.50%± 4.83%, 52.02%±4.63% in TBI group and 39.99%±4.99%, 34.87%±5.02%, 43.33%±4.54%, 37.84%±5.16% in TBI＋Gly group （all P〈0.01 compared with TBI group）. Conclusion： Gly can reduce secondary brain injury and improve outcomes in rat following TBI by down-regula- tion of HMGB 1/HMGB 1 receptors （TLR4 and RAGE）/NF- κB - mediated inflammatory responses in the injured rat brain.展开更多
基金Supported by Samsung Biomedical Research Institute grant,No.SBRI C-A8-219-1
文摘AIM： To examine how High-mobility group box I （HMGB1） regulates hepatocyte apoptosis and, furthermore, to determine whether glycyrrhizin （GL）, a known HMGB1 inhibitor, prevents HMGBl-induced hepatocyte apoptosis.
文摘Plants synthesize the osmoprotectant glycine betaine (GB) via choline→betaine aldehyde→glycine be- taine[1]. Two enzymes are involved in the pathway choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH). A full length CMO cDNA (1,643bp) was cloned from Amaranthus tricolor. The open reading frame encoded a 442-amino acid polypeptide, which showed 69% identity with CMOs in Spina- cia oleracea L. and Beta vulgaris L. DNA gel blot analysis indicated the presence of one copy of CMO gene in the A. tricolor genome. The expressions of CMO and BADH proteins in A.tricolor leaves significantly increased under salinization, drought and heat stress (42℃), as determined by immunoblot analysis, but did not respond to cold stress (4℃), or exogenous ABA application. The increase of GB content in leaves was parallel to CMO and BADH contents.
基金Supported by the Natural Science Foundation of Jilin Province (No. 990337).
文摘Bagasse fly ash was converted into an inexpensive adsorbent andutilized for the removal of β- naphthalenesulfonic acid in dilutesolution. The effect of pH, temperature, adsorbent concentration, andco-existed acids on the removal of β-naphthalenesulfonic acid wasexamined. The adsorption data have been correlated with both Langmuirand Freundlich adsorption models. Thermodynamic parameters obtainedindicate the feasibility of the process, and kinetic studies providedthe necessary mechanistic information of the removal process.
文摘A six-week growth trial was conducted to compare the effects of different feeding strate- gies of dietary immunostimulants on the growth and immunity of white shrimp Litopenaeus vannamei （4.70 ±0.20g）. Shrimps were fed with diet containing glycyrrhizin continuously, containing β -glucan continuously, discontinuously （seven days with diet containing β -gluseven days with diet without -glucan; two days with diet containing β-glucan following five days with diet without -glucan）,
文摘Sugar beet tops including leaves and crown harvested and chopped into 3-6 cm, mixed with sugar cane pulp and sugar cane molasses （about 5% of the total mass） to provide a mixture contained 30-35 percent dry mater. After two months of ensiling, the silage was evaluated and used in the ration of lactating buffaloes. A Latin square experiment in a changeover design, with three periods of time, each with 20 days for adaptation and 10 days for recording data, was conducted in which 15 multiparous lactating buffloes were used in three similar groups, each with five animals. The prepared silage was included in the diet of lactating buffaloes where a control diet （A） was compared with treatment diets contained 25% （B） and 50% （C） silage （DM basis） respectively. Milk yield was recorded, sampled and analysed for fat, protein, solid not-fat and total solids-content. The maximum daily rough milk yield per animal was obtained on diet C while minimum on diet A （P 〈 0.05）. Similar trends were observed for 4% fat corrected milk, and 3.5% fat corrected milk that were significantly different between the treatments （P 〈 0.05）. The percentage of crude fat and daily fat yield per animal were significantly （P 〈 0.05） higher in treatment C comparing to A and B. An increasingly trend of milk and fat yield was observed when the animals received silage contained diets but statistically it was significant only for the diets contained 50% silage. In addition, the fat percentage was significantly （P 〈 0.05） increased when the buffaloes received diet containing 50% silage. The highest （P 〈 0.05） amount of protein and total solid yield obtained, when the animals received high （50%） silage diet.
文摘Objective： To investigate the neuropro- tective effects of glycyrrhizin （Gly） as well as its effect on expression of high-mobility group box 1 （HMGB1） in rats after traumatic brain injury （TBI）. Methods： Male Sprague-Dawley rats were randomly divided into three groups： sham group, TBI group, and TBI＋Gly group （n=36 per group）. Rat TBI model was made by using the modified Feeney＇s method. In TBI＋Gly group, Gly was administered intravenously at a dosage of l0 mg/kg 30 min after TBI. At 24 h after TBI, motor function and brain water content were evaluated. Meanwhile, HMGB 1/HMGB 1 receptors including toll-like receptor 4 （TLR4） and receptor for advanced glycation end products （RAGE）/nuclear fac- tor-κ B（NF- κ B） signaling pathway and inflammatory cytokines in the injured brain tissues were detected using quantitative real-time polymerase chain reaction, western blot, electrophoretic mobility shift assay and enzyme-linked immunosorbent assay. Furthermore, HMGB l, RAGE and TLR4 immunohistochemistry and apoptosis were analyzed. Results： Beam walking performance impairment and brain edema were significantly reduced in TBI＋Gly group compared with TBI group; meanwhile, the over-expressions of HMGB 1PHMGB 1 receptors （TLR4 and RAGE）/NF- κB DNA-binding activity and inflammatory cytokines were inhibited. The percentages of HMGB 1, RAGE and TLR4- positive cells and apoptotic cells were respectively 58.37%±5.06%, 54.15%±4.65%, 65.50%± 4.83%, 52.02%±4.63% in TBI group and 39.99%±4.99%, 34.87%±5.02%, 43.33%±4.54%, 37.84%±5.16% in TBI＋Gly group （all P〈0.01 compared with TBI group）. Conclusion： Gly can reduce secondary brain injury and improve outcomes in rat following TBI by down-regula- tion of HMGB 1/HMGB 1 receptors （TLR4 and RAGE）/NF- κB - mediated inflammatory responses in the injured rat brain.
