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小学英语“生长型”单元复习课实践思考
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作者 吴臻一 《文理导航》 2024年第27期91-93,共3页
上好单元复习课,培养学生的英语学习兴趣、夯实语言知识、发展思维品质、提升学习质量都有着举足轻重的作用。本文分析了目前小学英语单元复习课存在的问题并结合课例分析如何让小学英语单元复习课更高效,促进学生“再生长”。
关键词 小学英语 单元复习课 “生长型”
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浅谈初中物理“生长型”课堂的有效构建
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作者 高千军 《学周刊》 2024年第27期52-54,共3页
物理学科具有较强的实用性,可以指导学生解决生活问题,提高学生的创造力。传统的物理教学方式存在弊端,限制学生的思维发展,违背了学生的成长规律。生长型课堂的出现极大地提高了物理教学质量,促进学生健康成长。初中物理教师要以学生... 物理学科具有较强的实用性,可以指导学生解决生活问题,提高学生的创造力。传统的物理教学方式存在弊端,限制学生的思维发展,违背了学生的成长规律。生长型课堂的出现极大地提高了物理教学质量,促进学生健康成长。初中物理教师要以学生为中心,打造学生喜闻乐见的物理学习环境,立足核心素养,践行生长课堂,助力师生共同成长。 展开更多
关键词 初中物理 “生长型”课堂 构建策略
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“生长型”大学校园规划探讨 被引量:8
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作者 张小松 周安伟 《规划师》 2004年第2期40-42,共3页
“生长型”校园规划应以旧校区的功能整合为基础,以新校区的空间拓展为关键,统一交通,共享资源,构筑校园特有的文化氛围,适应高校体制改革。
关键词 “生长型”校园 校园规划 高校 体制改革
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UBD理论视域下的“生长型”数学习题教学设计 被引量:3
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作者 宋予林 程永军 《数学教学通讯》 2023年第18期6-8,共3页
基于南京市雨花台中学建构“生长型”课堂的实践探索,结合UBD理论及多年的一线课堂教学实践,形成了“理解为先”“让学引思”的“生长型”数学习题教学设计模式,注重教学目标、教学内容生长的同时,更加注重教学评价对学生数学学习的理解... 基于南京市雨花台中学建构“生长型”课堂的实践探索,结合UBD理论及多年的一线课堂教学实践,形成了“理解为先”“让学引思”的“生长型”数学习题教学设计模式,注重教学目标、教学内容生长的同时,更加注重教学评价对学生数学学习的理解,以提高学生的解题能力,落实数学核心素养. 展开更多
关键词 理解为先 “生长型”课堂 让学引思 教学设计
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践行新课标理念,打造“生长型”课堂——新课标下的初中物理课堂模式探究
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作者 库力夏提·阿哈乃 《中文科技期刊数据库(全文版)教育科学》 2023年第5期165-167,共3页
在如今的教育体现下,更关注学生的能力和思维发展,也更关注于学生将知识转化为能力的过程。在此背景下,初中物理开展教学要注重“生长型”课堂的构建,关注学生的思维发育和能力发展,践行新课程标准理念。教师要围绕学生为主体,思考新课... 在如今的教育体现下,更关注学生的能力和思维发展,也更关注于学生将知识转化为能力的过程。在此背景下,初中物理开展教学要注重“生长型”课堂的构建,关注学生的思维发育和能力发展,践行新课程标准理念。教师要围绕学生为主体,思考新课程标准下的教学目标,注重对学生物理综合素养的培养。下文就新课程标准下,打造“生长型”的初中物理课堂教学模式进行分析与研究。 展开更多
关键词 新课标 初中物理 “生长型”课堂
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初中物理“生长型”课堂的构建方法
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作者 冯杰 《甘肃教育》 2024年第24期105-108,共4页
初中物理是一门理论性和实践性都较强的学科,其教学能够指导学生运用理论知识解决实际问题。然而,传统物理课堂往往以教师为中心,部分教师仍过于注重理论知识的传授和习题训练,强调学生的考试成绩,导致教学内容脱离生活实际,学生动手操... 初中物理是一门理论性和实践性都较强的学科,其教学能够指导学生运用理论知识解决实际问题。然而,传统物理课堂往往以教师为中心,部分教师仍过于注重理论知识的传授和习题训练,强调学生的考试成绩,导致教学内容脱离生活实际,学生动手操作能力差,不利于培养其兴趣、能力以及综合素质。为改变这一现状,文章将“生长型”课堂融入初中物理教学中,从传统“以教为中心”转变为“以学为中心”,有效提升学生的学习能力。具体从情境化教学、差异化教学、探究式教学和反思内化等四个方面,分析初中物理“生长型”课堂的构建方法。 展开更多
关键词 初中物理 “生长型”课堂 构建 方法
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快速原型制造技术——金属模具制造的新工艺 被引量:2
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作者 蔡悦华 廖德刚 刘文清 《常德师范学院学报(自然科学版)》 2000年第2期52-53,共2页
叙述了全新的“生长型”制造技术 (MIM)———快速原型制造技术的原理及其特点 ,介绍了几种常用的快速原型工艺 ,并给出了选择性激光烧结SLS、分层实体制造LOM和熔融堆积成形FDM等快速原型制造技术在金属模具制造中的应用途径 ,指出了... 叙述了全新的“生长型”制造技术 (MIM)———快速原型制造技术的原理及其特点 ,介绍了几种常用的快速原型工艺 ,并给出了选择性激光烧结SLS、分层实体制造LOM和熔融堆积成形FDM等快速原型制造技术在金属模具制造中的应用途径 ,指出了快速原型制造技术在金属模具制造中存在的局限性 ,并对其发展前景作出展望。 展开更多
关键词 金属模具制造 快速原制造技术 “生长型”制造技术 快速原工艺 熔融堆积成形 立体光刻 分层实体制造
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适合教师的个性化专业发展方式 被引量:11
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作者 史静寰 王振权 《教育理论与实践》 CSSCI 北大核心 2013年第4期35-39,共5页
个性化专业发展基于为每一个教师提供适合的专业发展方式及理念,立足于个性化的教师教育方式,思考和实践从"需求导向"出发,以适合教师自身专业发展的方式,引导和满足教师多样化专业发展的要求,探索"轻负担、高质量、可持... 个性化专业发展基于为每一个教师提供适合的专业发展方式及理念,立足于个性化的教师教育方式,思考和实践从"需求导向"出发,以适合教师自身专业发展的方式,引导和满足教师多样化专业发展的要求,探索"轻负担、高质量、可持续"的"生长型"专业发展新路,通过个性化的专业引领,帮助每一位教师自主建构并实现从"实践者-行动研究者-专业领导者"的角色转型。 展开更多
关键词 教师 个性化专业发展 “生长型”专业发展
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历史街区的链接式保护及生长型发展 被引量:2
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作者 赵亮 娄淑娟 +1 位作者 李怡谦 赵健 《城市问题》 CSSCI 北大核心 2008年第8期23-28,共6页
以历史街区的保护与发展模式为研究对象,阐述了历史街区与现代城市割裂的现状,通过对国内各种模式的理论研究与实践经验的分析,引发了对历史街区保护与发展模式的新探讨,进而提出了"链接式"保护模式及"生长型"发展... 以历史街区的保护与发展模式为研究对象,阐述了历史街区与现代城市割裂的现状,通过对国内各种模式的理论研究与实践经验的分析,引发了对历史街区保护与发展模式的新探讨,进而提出了"链接式"保护模式及"生长型"发展模式的思路,并结合济南老城区"芙蓉街—曲水亭街街区"的实例,分别从传统街巷联系、历史与现代的衔接、空间布局、建筑及文脉处理等方面探讨了该模式的应用。 展开更多
关键词 历史街区 “链接式”保护模式 “生长型”发展模式 芙蓉街-曲水亭街街区
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Comparison of stand structure and growth between artificial and natural forests of Pinus sylvestiris var. mongolica on sandy land 被引量:22
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作者 朱教君 范志平 +2 位作者 曾德慧 姜凤岐 MATSUZAKI Takeshi 《Journal of Forestry Research》 SCIE CAS CSCD 2003年第2期103-111,共9页
Mongolian pine (Pinus sylvestiris Linnaeus var. mongolica Litvinov) as a valuable conifer tree species has been broadly introduced to the sandy land areas in 揟hree North?regions (North, northwest and northeast of Chi... Mongolian pine (Pinus sylvestiris Linnaeus var. mongolica Litvinov) as a valuable conifer tree species has been broadly introduced to the sandy land areas in 揟hree North?regions (North, northwest and northeast of China), but many problems occurred in the earliest Mongolian pine plantations in Zhanggutai, Zhangwu County, Liaoning Province (ZZL). In order to clarify the reason, comprehensive investigations were carried out on differences in structure characteristics, growth processes and ecological factors between artificial stands (the first plantation established in ZZL in 1950s) and natural stands (the origin forests of the tree species in Honghuaerji, Inner Mongolia) on sandy land. The results showed that variation of diameter-class distributions in artificial stands and natural stands could be described by Weibull and Normal distribution models, respectively. Chapman-Richards growth model was employed to reconstruct the growth process of Mongolian pine based on the data from field investigation and stem analysis. The ages of maximum of relative growth rate and average growth rate of DBH, height, and volume of planted trees were 11, 22 years, 8, 15 years and 35, 59 years earlier than those of natural stand trees, respectively. In respect of the incremental acceleration of volume, the artificial and natural stands reached their maximum values at 14 years and 33 years respectively. The quantitative maturity ages of artificial stands and natural stands were 43 years and 102 years respectively. It was concluded that the life span of the Mongolian pine trees in natural stands was about 60 years longer than those in artificial stands. The differences mentioned above between artificial and natural Mongolian pine forests on sandy land were partially attributed to the drastic variations of ecological conditions such as latitude, temperature, precipitation, evaporation and height above sea level. Human beings' disturbances and higher density in plantation forest may be ascribed as additional reasons. Those results may be potentially useful for the management and afforestation of Mongolian pine plantations on sandy land in arid and semi-arid areas. 展开更多
关键词 Pinus sylvestiris var. mongolica Mongolian pine Sandy land COMPARISON Growth model
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SAR Data Assimilation for Crop Biomass Simulation Based on Crop Growth Model 被引量:3
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作者 谭正 刘湘南 +1 位作者 张晓倩 吴伶 《Agricultural Science & Technology》 CAS 2012年第5期1127-1132,共6页
Biomass from SAR data was assimilated into crop growth model to describe relationship between crop biomass and crop growth time to improve estimation accuracy of biomass. In addition, inverse model was established in ... Biomass from SAR data was assimilated into crop growth model to describe relationship between crop biomass and crop growth time to improve estimation accuracy of biomass. In addition, inverse model was established in order to estimate biomass according to relationship between biomass and backscattering coefficients from SAR data. Based on cost function, parameters of growth model were optimized as per conjugate gradient method, minimizing the differences between estimated biomass and inversion values from SAR data. The results indicated that the simulated biomass using the revised growth model with SAR data was consistent with the measured one in time distribution and even higher in accuracy than that without SAR data. Hence, the key parameters of crop growth model could be revised by real-time growth information from SAR data and accuracy of the simulated biomass could be improved accordingly. 展开更多
关键词 Data assimilation BIOMASS SAR Crop growth model
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Comparative Analysis on Growth and Morphology in Yangtze River ♀,Zhujiang River ♂ and Their F_1 Hybrids of Juvenile Grass Carp 被引量:6
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作者 傅建军 王荣泉 +3 位作者 刘峰 宣云峰 朱树人 李家乐 《Agricultural Science & Technology》 CAS 2009年第5期119-122,共4页
[ Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR (Yangtze River stock ♀×Yangtze River... [ Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR (Yangtze River stock ♀×Yangtze River stock ♂ ), ZR (Zhujiang River stock ♀ ×Zhujiang River stock♂ ) and hybrids F1 ( Yangtze River stock ♀ × Zhujiang River stock ♂ ) were established, and their growth or morphological data were also measured. [ Result] The orders of body weight, standard length and absolute growth rate were F1 〉 ZR 〉 YR. No significant difference was found in body weight or standard length of 50 day-old generations ( P〉0. 05), while there was an extremely significantly difference in those of 170 day-old generations ( P 〈0.01 ). However, the absolute growth rate of F1 was 20.00% and 50.00% higher than that of ZR and YR respectively, and no significant difference was found between F1 and ZR ( P 〉 0.05), but significant difference between F1 and YR ( P 〈 0.05). F1 showed a significant hybrid vigor with rate of 20.09%. There was no significant difference among three inbred generations in standard length/total length ( P 〉 0.05), while significant difference in head length/total length, body height/standard length and body width/standard length ( P 〈 0.05), which indicated that F1 had the characteristics of shorter head, higher and wider body. [ Conclusion]F1 has advantages in growth performance and morphological characteristics. 展开更多
关键词 Grass carp Hybrid Growth performance Morphological parameter
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Porcine Breeding Management in a Large-scale Piggery with Microbial Fermentation Bed 被引量:1
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作者 刘波 李兆龙 +6 位作者 唐建阳 黄勤楼 郑回勇 蓝江林 史怀 翁伯琦 余文权 《Agricultural Science & Technology》 CAS 2015年第1期160-166,171,共8页
[Objective] The behavior of eating, drinking, defecating and peeing of 1 500 pigs in a large-scale microbial fermentation bed-equipped piggery was observed. We hoped to find some simple indicators that could reflect t... [Objective] The behavior of eating, drinking, defecating and peeing of 1 500 pigs in a large-scale microbial fermentation bed-equipped piggery was observed. We hoped to find some simple indicators that could reflect the health status of swinery and to provide experience for the swinery performance management in large-scale microbial fermentation bed-equipped piggery. [Method] The body weight (BW), daily BW gain, feed intake and other indicators of different-day-old pigs were recorded in details. Based on the recorded data, the models between BW, BW gain, average daily feed intake and feed/gain ratio and growth days (d) were established. In addition, the incidences of pox-like macula (dermatitis), diarrhea (gastrointestinal disease), cough (respiratory disease), stiff pig (malnutrition), conjunctivitis (eye disease) and foot inflection (trauma) among fattening pigs were also investigated. [Result] The BW range, average BW, daily BW gain, breeding days, daily feed intake range, average daily feed intake, staged feed intake, accumulated feed intake, feed/gain ratio and accumulated feed/gain ratio of different-day-old pigs were studied, respectively. Four dynamic models were established for the growth of pigs: (1) the BW (y)-age (x) mod- el: y=0.758 9x-19.883 (3=0.993 7); (2) the BW gain (y)-age (x) model: y=1.039 5x05051 (F=0.885 4); (3) the average daily feed intake (y)-age (x) model: y=0.023 5x-0.334 3 (F=0.991 7); (4) the feed/gain ratio (y)-age (x) model: y=0.022x+0.427 8 (P=0.988 5). Based on these models, the corresponding theoretical growth value of pigs at different growth stage could be predicted. The main diseases occurred among the swinery in the large-scale microbial fermentation bed piggery included pox-like macula (dermatitis), diarrhea (gastrointestinal disease), cough (respiratory disease), stiff pig (mal- nutrition), conjunctivitis (eye disease) and foot inflection (trauma). The deadly infec- tious diseases had been not found among the pigs. [Conclusion] When the actual BW, BW gain, average daily feed intake and feed/gain ratio were all lower than the theoretical values predicted by the models, the management should be enhanced. The average daily feed intake of 60 to 65-day-old pigs was lower than the theoretic value, indicating that the pigs could not adapt nicely to the fermentation bed at the very early stage. When the pigs grew up to 70 to 75 d old, the average daily feed intake was higher than the theoretical value, indicating that the pigs had adapted to the fermentation bed. In particularly, average daily feed intake of 75-day-old pigs was higher than the theoretical value by 21%. It was suggested the fermentation bed was conducive to the growth of pigs. Considering the occurrence of diseases among pigs, the overall incidence was relatively low. The incidence of each disease was all lower than 10% with little difficulty in treating. If the management of mattress was strength- ened, such as paying attention to feeding and keeping water clean, many diseases could heal by themselves. 展开更多
关键词 Disease investigation Large-scale breeding Microbial fermentation bed Porcine growth model
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Comparative study on three dynamic modulus of elasticity and static modulus of elasticity for Lodgepole pine lumber 被引量:5
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作者 梁善庆 傅峰 《Journal of Forestry Research》 SCIE CAS CSCD 2007年第4期309-312,共4页
The dynamic and static modulus of elasticity (MOE) between bluestained and non-bluestained lumber of Lodgepole pine were tested and analyzed by using three methods of Non-destructive testing (NDT), Portable Ultras... The dynamic and static modulus of elasticity (MOE) between bluestained and non-bluestained lumber of Lodgepole pine were tested and analyzed by using three methods of Non-destructive testing (NDT), Portable Ultrasonic Non-destructive Digital Indicating Testing (Pundit), Metriguard and Fast Fourier Transform (FFT) and the normal bending method. Results showed that the dynamic and static MOE of bluestained wood were higher than those of non-bluestained wood. The significant differences in dynamic MOE and static MOE were found between bulestained and non-bluestained wood, of which, the difference in each of three dynamic MOE (Ep. the ultrasonic wave modulus of elasticity, Ems, the stress wave modulus of elasticity and El, the longitudinal wave modulus of elasticity) between bulestained and non-bluestained wood arrived at the 0.01 significance level, whereas that in the static MOE at the 0.05 significance level. The differences in MOE between bulestained and non-bluestained wood were induced by the variation between sapwood and heartwood and the different densities of bulestained and non-bluestained wood. The correlation between dynamic MOE and static MOE was statistically significant at the 0.01 significance level. Although the dynamic MOE values of Ep, Em, Er were significantly different, there exists a close relationship between them (arriving at the 0.01 correlation level). Comparative analysis among the three techniques indicated that the accurateness of FFT was higher than that of Pundit and Metriguard. Effect of tree knots on MOE was also investigated. Result showed that the dynamic and static MOE gradually decreased with the increase of knot number, indicating that knot number had significant effect on MOE value. 展开更多
关键词 Lodgepole pine Non-destructive testing Dynamic modulus of elasticity Static modulus of elasticity
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Studies on stand dynamic growth model for larch in Jilin in China 被引量:1
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作者 翁国庆 陈雪峰 《Journal of Forestry Research》 SCIE CAS CSCD 2004年第4期323-326,共4页
The stand growth and yield dynamic models for Larch in Jilin Province were developed based on the forest growth theories with the forest continuous inventory data. The results indicated that the developed models had h... The stand growth and yield dynamic models for Larch in Jilin Province were developed based on the forest growth theories with the forest continuous inventory data. The results indicated that the developed models had high precision, and they could be used for the updating data of inventory of planning and designing and optimal decision of forest management. 展开更多
关键词 Stand Dynamics Growth Prediction Model
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A Simplified Model with Soil Water Limitation on Spring Wheat Growth 被引量:2
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作者 郑海雷 米谷俊颜 黄子琛 《Acta Botanica Sinica》 CSCD 2000年第5期512-517,共6页
The authors constructed a simplified model of spring wheat (Triticum aestivum L.) carbon assimilation and dry matter accumulation (DMA) process which consisted of two independent variables, day length (L) and total... The authors constructed a simplified model of spring wheat (Triticum aestivum L.) carbon assimilation and dry matter accumulation (DMA) process which consisted of two independent variables, day length (L) and total daily radiation (TDR). Leaf water potential (Ψ) was incorporated into the simplified growth model based on the assumption that both light use efficiency (α) and CO 2 conductance of assimilation (g c) were depressed by water limitation. Finally,Ψ was estimated from a regression equation in which the independent variables were relative soil water content in the upper 80 cm (θ R,80 ), ambient temperature (T a), vapor pressure deficit (VPD), the cumulative leaf water potential below thresholds of -1.5 MPa (Ψ c,1.5 ). Some applications in research program of field experiment of atmosphere_land surface processes in Heihe River region were tested. The simulated data agreed well with the data observed at Linze oasis in 1989 for various levels of water supply and at Zhangye oasis in 1992 in the field. The analysis and simulation using the model demonstrated that the simplified growth model could describe very well the DMA process of spring wheat with and without water limitation in the region of HEIFE (Heihe field experiment). 展开更多
关键词 spring wheat GROWTH simplified model water limitation
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Microstructure and crystal growth direction of Al-Cu alloy 被引量:1
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作者 陈体军 李向威 +1 位作者 郭海洋 郝远 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2015年第5期1399-1409,共11页
The microstructures and crystal growth directions of permanent mould casting(PMC) and directionally solidified(DS) Al-Cu alloys with different contents of Cu were investigated. Simultaneously, the effects of pouri... The microstructures and crystal growth directions of permanent mould casting(PMC) and directionally solidified(DS) Al-Cu alloys with different contents of Cu were investigated. Simultaneously, the effects of pouring temperature on the microstructure and crystal growth direction of permanent mould casting pure Al were also discussed. The results indicate that the α(Al) crystals in the pure Al do not always keep common columnar grains, but change from the columnar grains to columnar dendrites with developed arms as the pouring temperature rises. The growth direction also varies with the change of pouring temperature. Cu element has similar effects on the microstructures of the PMC and DS casting Al-Cu alloys and the α(Al) crystals gradually change from columnar crystals in turn to columnar dendrites and developed equiaxed dendrites as the Cu content increases. The crystal growth direction in the PMC alloys gradually approaches (110) orientation with increasing Cu content. But the resulting crystals with growth direction of (110) do not belong to feathery grains. There are also no feathery grains to form in all of the DS Al-Cu alloys. 展开更多
关键词 Al-Cu alloy directional solidification crystal growth direction permanent mould casting MICROSTRUCTURE
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Simulation Study on the Alkalized-salinized Grassland Ecosystem in the Songnen Plain 被引量:1
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作者 尚宗波 高琼 李建东 《Acta Botanica Sinica》 CSCD 2001年第6期624-630,共7页
Using Object-oriented design and a new programming language JAVA, a physically-based model was built to simulate the hydrological, alkalization/de-alkalization and salinization/desalinization processes in soil. Furthe... Using Object-oriented design and a new programming language JAVA, a physically-based model was built to simulate the hydrological, alkalization/de-alkalization and salinization/desalinization processes in soil. Furthermore, a process-based model was built to evaluate the dynamics of four herbaceous ecosystems (including dynamics of above-ground biomass, below-ground biomass, and litter biomass), each dominated by Aneurolepidium chinense (Trin.) Kitag., Chloris virgata Sw., Puccinellia tenuiflora (Turcz.) Scribn. et Merr. and Suaeda glauca Bunge. This model is a daily-time step model, suitable for simulating hydrological, alkalization/de-alkalization and salinization/desalinization processes of heterogeneous soil, and growth dynamics of different grassland communities. With climatic data and experimental data of Changling Experimental Site in Jilin Province, the soil moisture content (in 1991, 1996, 1997 and 1998), soil salt concentration, exchangeable cation percentage and pH in soil and growth dynamics of these four sorts of grassland communities (in 1991) were simulated and the results were verified to be in accord with observed data. 展开更多
关键词 alkalinized-salinized grassland hydrological process alkalization/de-alkalization processes salinization/desalinization processes growth dynamics ecological model
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Biological Characteristics of Biosurfactantproducing Petroleum-degrader Bacterium Bacillus BS-8
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作者 常慧萍 邢文会 +3 位作者 夏铁骑 付瑞敏 陶令霞 张红 《Agricultural Science & Technology》 CAS 2016年第1期1-3,18,共4页
The growth characteristics of petroleum-degrader BS-8(Bacillus sp.) and the factors influencing its biosurfactant production were tested; the biosurfactant releasing mode of BS-8 was speculated by measuring OD600, s... The growth characteristics of petroleum-degrader BS-8(Bacillus sp.) and the factors influencing its biosurfactant production were tested; the biosurfactant releasing mode of BS-8 was speculated by measuring OD600, surface tension and oil displacement of fermentation broth; and the effects of carbon source, nitrogen source, temperature, p H, and Na Cl concentration on biosurfactant production by BS-8 were observed in this study. The results showed that the biosurfactant releasing mode of BS-8 was growth-correlated, the surface tension of BS-8 fermentation broth declined with the total bacteria increasing, and the oil displacement was positive correlated with biosurfactant content in fermentation broth; and the optimal culture conditions for effective biosurfactant production included: glucose as carbon source,yeast extract as nitrogen source, Na Cl concentration of 2%, p H of 7.0 and temperature at 30 ℃. 展开更多
关键词 BIOSURFACTANT Petroleum-degrader BS-8 Growth-correlated Biological characteristic
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Cell Growth and Ajmalicine Accumulation in a Full Habituated Catharanthus roseus Cell Line C_ (20)hi 被引量:2
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作者 郑珍贵 周煜 +1 位作者 刘涤 胡之璧 《Acta Botanica Sinica》 CSCD 2002年第10期1146-1150,共5页
A full habituated cell line C_ 20hi was screened from 2,4_D dependent line (C_ 20D) of Catharanthus roseus (L.) G. Don. The investigation involved the cell growth, ajmalicine production and enzyme activity related t... A full habituated cell line C_ 20hi was screened from 2,4_D dependent line (C_ 20D) of Catharanthus roseus (L.) G. Don. The investigation involved the cell growth, ajmalicine production and enzyme activity related to indole alkaloid biosynthesis in both cell lines. These results indicated that C_ 20hi cells grew faster than C_ 20D cells, and average ajmalicine content in C_ 20hi cells was 18.4 times more than that in C_ 20D when cultured in the production medium. In the growth medium, average ajmalicine content in C_ 20hi cells was 31.9 times more than that in C_ 20D cells, while the cell growth has no obvious difference. The comparison of enzyme activities in C_ 20hi and C_ 20D cells indicated that tryptophan decarboxylase (TDC), strictosidine synthase (SSS) and geraniol_10_dehydrogenase (G10H) activities have no close relation to ajmalicine accumulation, although the activities of these enzymes were higher when cells were cultured in the production medium than in the growth medium. The C_ 20hi cells are relatively stable in five years of culture. 展开更多
关键词 full habituated cells AJMALICINE cell culture Catharanthus roseus
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